PERBANDINGAN UJI TETRAZOLIUM DAN RADICLE EMERGENCE DALAM MENDUGA VIABILITAS BENIH KOPI ARABIKA (Coffea arabica L.) / The Comparison of Tetrazolium and Radicle Emergence Test to Estimate the Viability of  Arabica Coffee (Coffea arabica L.) Seeds

Development of seed testing methods needs to be conducted in order to obtain fast, precise and applicable result in the field. The objective of this study was to compare the two rapid test methods of Arabica coffee seed viability, the tetrazolium and radicle emergence tests. The experiment was conducted at Quality Seed Testing Laboratory, Balai Besar Perbenihan dan Proteksi Tanaman Perkebunan Surabaya from beginning of January until March 2018. Three lots of Arabica coffee (Coffea arabica var. Sigarar Utang) (Lot A = harvest in 1st week of August, 2017; Lot B = 3rd week of August, 2017; and Lot C = 1st week of September, 2017) were obtained from Indonesian Coffee and Cocoa Research Institute, Jember. The experiments used completely randomized design with four replications. The result showed that the viability test using TZ was faster (4 days) than using RE (14 days). Regression equation models for estimating seed viability are less precise, while the estimation method of paired sample t-test was sufficient to predict the actual value of the seed viability. Redicle emergence is longer than tetrazolium which is for 14 days. The regression equation model of the radicle emergence is sufficient to predict the seed physiological quality parameter.Keywords: viability, tetrazolium, radicle emergence, coffee seed AbstrakPengembangan metodologi uji benih untuk memperoleh hasil yang cepat, tepat dan aplikatif di lapangan. Tujuan penelitian adalah membandingkan dua metode pengujian viabilitias benih kopi Arabika, yaitu uji tetrazolium dan radicle emergence (kemunculan radikula). Penelitian dilaksanakan di Laboratorium Pengujian Mutu Benih, Balai Besar Perbenihan dan Proteksi Tanaman Perkebunan Surabaya, mulai bulan Januari sampai Maret 2018. Tiga lot benih kopi Arabika varietas Sigarar Utang (Lot A : panen minggu I bulan Agustus 2017, Lot B : minggu III bulan Agustus 2017, Lot C : minggu I bulan September 2017) diperoleh dari Pusat Penelitian Kopi dan Kakao Indonesia. Rancangan yang digunakan adalah rancangan acak lengkap satu faktor (lot benih) dengan empat kali ulangan. Hasil penelitian menunjukkan bahwa pengujian viabilitas kopi dengan metode TZ lebih cepat (4 hari) dibandingkan dengan metode kemunculan radikula (14 hari). Penggunaan model regresi untuk menduga viabilitas biji kurang tepat, sementara penggunaan t-test berpasangan sangat tepat untuk menduga nilai yang sebenarnya dari viabilitas biji. Oleh karena itu, metode pendugaan mutu benih kopi Arabika berdasarkan kemunculan radikula lebih tepat untuk digunakan di lapangan.Kata kunci: viabilitas, tetrazolium, kemunculan radikula, benih kopi

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PENGARUH MEDIA TANAM TERHADAP PERKECAMBAHAN BENIH KOPI ARABIKA (Coffea arabica L)

Jurnal AGROSAINS dan TEKNOLOGI ◽

10.24853/jat.4.2.64-69 ◽

2020 ◽

Vol 4 (2) ◽

pp. 64

Author(s):

Yana Taryana ◽

Lia Sugiarti

Keyword(s):

Seed Germination ◽

Coffea Arabica ◽

Block Design ◽

Mixed Media ◽

Arabica Coffee ◽

Randomized Block Design ◽

The Media ◽

Coffea Arabica L ◽

Coffee Seed ◽

Experimental Garden

A study to determine the effect of various types of mixed planting media on Arabica coffee seed germination was carried out in April to May 2019, located in the Experimental Garden of the Faculty of Agriculture, University of Winaya Mukti, Tanjungsari, Sumedang. This study used a randomized block design (RBD) consisting of 4 treatments and 5 replications. The planting media used in this study were mixed media consisting of A = soil; B = mixture of soil and manure (1: 1); C = mixture of soil and charcoal (1: 1); D = mixture of soil, manure and husk charcoal (1: 1: 1), The results of the study showed the planting media in the form of soil, a mixture of soil with manure or charcoal husk, and the mixture of the three did not affect the germination of seeds, but the use of in the form of a mixture of soil with manure and husk charcoal can increase the germination index compared to the media in the form of soil without mixture.

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Cryopreservation of Coffea arabica L. Zygotic Embryos by Vitrification

Notulae Botanicae Horti Agrobotanici Cluj-Napoca ◽

10.15835/nbha44210553 ◽

2016 ◽

Vol 44 (2) ◽

pp. 445-451 ◽

Cited By ~ 2

Author(s):

Rodrigo Therezan de FREITAS ◽

Renato PAIVA ◽

Thais Silva SALES ◽

Diogo Pedrosa CorrÃªa da SILVA ◽

Michele ValquÃria dos REIS ◽

...

Keyword(s):

Coffea Arabica ◽

Seed Storage ◽

Anatomical Study ◽

Embryo Cryopreservation ◽

Zygotic Embryos ◽

Two Temperatures ◽

Vitrification Solution ◽

Cryoprotectant Solution ◽

Coffea Arabica L ◽

Coffee Seed

As a consequence of the difficulty in conventional coffee seed storage, biotechnological alternatives such as cryopreservation have been investigated. The objective of this study was to develop a protocol for the cryopreservation of Coffea arabica L. (cv. â€˜CatuaÃ Vermelhoâ€™ - IAC 144) zygotic embryos by vitrification. For the cryopreservation study, the embryos were immersed in Plant Vitrification Solution 2 at different times (0, 10, 25, 50, 100, and 250 min) and two temperatures (0 and 25 Â°C). Subsequently, the best thawing time was determined in a water bath (1, 3, 5 minutes or directly in Recovery Solution). An anatomical study was conducted on non-stored and stored embryos, with or without the use of Plant Vitrification Solution 2. The immersion in cryoprotectant solution for 100 min at 0 Â°C allows embryo cryopreservation. Embryos can be directly thawed in Recovery Solution after storage in liquid nitrogen. It was observed that Plant Vitrification Solution 2 reduced internal water content in the cells, allowing subsequent embryo growth resumption.

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The effect of auxin 2,4-D and cytokinin 2-ip on direct somatic embryogenesis formation of Coffea arabica L. leaf explant

Pelita Perkebunan (a Coffee and Cocoa Research Journal) ◽

10.22302/iccri.jur.pelitaperkebunan.v27i2.146 ◽

2011 ◽

Vol 27 (2) ◽

Author(s):

Rina Arimarsetiowati

Keyword(s):

Tissue Culture ◽

Somatic Embryogenesis ◽

Coffea Arabica ◽

Callus Formation ◽

Culture Technique ◽

Plant Production ◽

Direct Somatic Embryogenesis ◽

Arabica Coffee ◽

Randomized Design ◽

Coffea Arabica L

One of the propagation technique for coffee plant production is tissue culture. Tissue culture technique for Coffea arabica L. faces some problems, mainly in the planlet formation regenerated from explants. The objective of this experiment was to examine the effect 2,4-D and 2-ip combination on the formation of direct somatic embryogenesis of Coffea arabica L. in leaves explant. Auxin (2,4-D) and cytokinin (2-ip) concentrations of, respectively, 1; 5 µM and 5; 10; 15; 20 were used as treatments. This research was conducted using completely randomized design with 10 replications. Observation to induce somatic embryos was done by quantitatively on number of callus from explant and number of embryogenic callus. Beside that, observation by qualitative descriptive was also done on deve lopment of embryogenesis. The results showed that Arabica coffee leaves explant of AS 2K clones could be induced in all medium combination except 5µM 2,4-D and 20µM 2-ip combination. Arabica coffee leaves explant of S 795, Sigararutang and AS 1 varieties could be induced in all medium combination. The highest frequency of callus formation was found in AS 2K, Sigararutang and AS 1 varieties on medium containing 1µM 2,4-D in combination with 10µM 2-ip, whereas for the S 795 variety on medium containing 5µM 2,4-D in combination with 10µM 2-ip. The highest frequency of embriogenic callus in all Arabica coffee variety could be reached on medium containing 5µM 2,4-D in combination with 15µM 2-ip. Key words : Coffea arabica L., somatic embryogenesis, 2,4-D, 2-ip, tissue culture, leaves, callus embryogenic.

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Teratogenic Effects of Arabica Coffee (Coffea arabica L) on Rats During the Organogenesis Period

Indonesian Journal of Pharmaceutical and Clinical Research ◽

10.32734/idjpcr.v3i2.4311 ◽

2021 ◽

Vol 3 (2) ◽

pp. 25-31

Author(s):

Reza Destri Anggi ◽

Yuandani ◽

Aminah Dalimunthe

Keyword(s):

Weight Loss ◽

Body Length ◽

Coffea Arabica ◽

Teratogenic Effect ◽

Teratogenic Effects ◽

Arabica Coffee ◽

Coffea Arabica L

The purpose of the research was to evaluate the teratogenic effect caused by theprovision of Arabica coffee solution with variations of 0.36 ml, 0.72 ml and 1.08 ml withpositive control of caffeine at a dose of 300 mg / kg bw to rats during organogenesis. Themeasurement parameters in this study are the appearance of reproduction, externalmalformations and scalal malformations. The results in this study found that theadministration of Arabica coffee solution with a volume of 1.08 ml and caffeine 300 mg /kg bb caused an abnormality in the reproductive appearance of weight loss and body length,whereas for extrenal malformation and scalal malforation no abnormalities were found ineach administration of the solution Arabica coffee and caffeine.

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Formulation and evaluation of body splash from Java preanger Arabica coffee (Coffea arabica L.) oil

Journal of Physics Conference Series ◽

10.1088/1742-6596/1402/5/055092 ◽

2019 ◽

Vol 1402 ◽

pp. 055092

Author(s):

R Handayani ◽

N Auliasari ◽

T K Oktaviany ◽

S Hindun ◽

F F Sriarumtias

Keyword(s):

Coffea Arabica ◽

Arabica Coffee ◽

Coffea Arabica L

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Ethylene-induced fruit ripening in arabica coffee (Coffea arabica L.)

Australian Journal of Experimental Agriculture ◽

10.1071/ea9920401 ◽

1992 ◽

Vol 32 (3) ◽

pp. 401 ◽

Cited By ~ 6

Author(s):

EC Winston ◽

M Hoult ◽

CJ Howitt ◽

RK Shepherd

Keyword(s):

Coffea Arabica ◽

Leaf Abscission ◽

Maturity Stage ◽

Early Application ◽

Arabica Coffee ◽

Fruit Abscission ◽

Time Of Application ◽

Berry Weight ◽

Berry Ripening ◽

Coffea Arabica L

The effects of ethephon on arabica coffee (Coffea arabica L.) were tested in experiments conducted over 2 years in North Queensland. Rates tested were 0, 125, 250, 500, 750, 1000, 1500, and 2000 mgkg, applied when 15-20% (early treatment) or 45-50% (late treatment) of fruit were coloured. Measurements were made on the degree of leaf and fruit abscission, berry ripening, and berry weight. Ethephon did not cause significant fruit abscission, but rates >500 mg/kg caused unacceptable leaf abscission. Rates of 250 mg/kg were marginally acceptable in terms of leaf abscission. Excessive defoliation led to dieback, which resulted in a reduced crop the following season. Ethephon concentrated berry ripening, significantly increased the number of red (mature) berries, and decreased the number of green (immature) berries. Rates of >500 mg/kg were effective in accelerating berry ripening, while 125 mg/kg had a limited effect. Maturity stage of the bean at time of application helped to determine the ethephon response; early application of ethephon had a more pronounced effect on berry ripening than late application. Ethephon applied early at rates of 1500 and 2000 mg/kg decreased fresh berry weight compared with all other treatments but parchment weight was unaffected. Selection of a suitable rate of ethephon from these data is difficult because berry ripening is offset by leaf drop. Variability of response was also observed.

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