Effect of plant growth regulators on callus induction and Rutin production of Ricinus communis L. plant

Ricinus communis L. is an important medical plant hence it contains many active compounds. The aim of this research is to study the effect of plant growth regulators on callus induction and Rutin concentration. A combination of Benzyle adenine (BA) and Indol Acetic acid (IAA) at (0.0,1.0,2.0) mg/L was added to the media, the highest fresh weight of the induced callus from stem explant was (4.97) gr . at (1.0,1.0) mg/L BA and IAA consenquently the same combination gave the highest dry weight of callus (0.42) gr. while the combination at (2.0,1.0) mg/L BA and IAA gave the highest fresh weight of induced callus from Leaves explant (5.28) gr., then (2.0,1.0) mg/L BA and IAA gave the highest dry weight for callus induced from leaves at (0.55)gr.Spectrophotometer used to estimate rutin quantity and results showed that the present of rutin at (126.31) ppm in callus induced from stem at (2.0,2.0) mg/L BA and IAA, the highest value of this compound (121.05) ppm on callus induction from the leaves in the same combination as compared with Rutin quantity in intact plant that reached (94.73) ppm in leaves and (68.42) ppm in stem.

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Effect of plant growth regulators on callus induction and Rutin production of Ricinus communis L. plant

Baghdad Science Journal ◽

10.21123/bsj.2017.14.3.0461 ◽

2017 ◽

Vol 14 (3) ◽

Keyword(s):

Plant Growth ◽

Plant Growth Regulators ◽

Growth Regulators ◽

Callus Induction ◽

Ricinus Communis ◽

Ricinus Communis L

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Callus Induction of Gendarussa (Justicia gendarussa) by Various Concentration of 2,4-D, IBA, and BAP

Biosaintifika Journal of Biology & Biology Education ◽

10.15294/biosaintifika.v9i3.11347 ◽

2017 ◽

Vol 9 (3) ◽

pp. 402 ◽

Cited By ~ 1

Author(s):

Dwi Kusuma Wahyuni ◽

Putri Andriani ◽

Arif Nur Muhammad Ansori ◽

Edy Setiti Wida Utami

Keyword(s):

Plant Growth ◽

Plant Growth Regulators ◽

Growth Regulators ◽

Callus Induction ◽

Callus Formation ◽

Leaf Explants ◽

Culture Method ◽

Dry Weight ◽

Fresh Weight ◽

Two Factors

Justicia gendarussa Burm.f., a medicinal plant, is Acanthaceae that has many functions. Furthermore, the compounds in gendarussa must be produced in high quantity and quality by applying callus culture method. Accordingly, it is important to study the effects of plant growth regulators (2,4-D, IBA, and BAP) on callus induction of gendarussa leaves. This research design utilized a factorial design with two factors (2,4-D and IBA: 0.5, 1, 1.5 mg/L and BAP: 0.5, 1, 1.5, 2 mg/L). The experiment consisted of 24 treatments, each of which was repeated 3 times. Observation was carried out in 6 weeks. Data on the time of callus formation, percentage of explants formed callus, and callus morphology were analyzed descriptively,while data on fresh and dry weight were analyzed by Two-Way ANOVA (α = 0.5). Interestingly, the results showed that various concentration of plant growth regulators (2,4-D, IBA, and BAP) affected callus induction from leaf explants of gendarussa. We concluded that the most optimal treatment combination of concentration of plant growth regulators in inducing callus from leaf explants of gendarussa is 1.5 mg/L 2,4-D and 2 mg/L BAP with a relatively long period of callus formation at the earliest, i.e. on day 5, 2.247 g of fresh weight, 0.108 gof dry weight, white callus translucent, and friable. Moreover, the optimum treatment will be used to produce secondary metabolite and seed synthetic by cell suspension culture.

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Susceptibility in the Growth of Viola tricolor `Blue' to Plant Growth Regulators and Fish Emulsion

HortScience ◽

10.21273/hortsci.30.4.852d ◽

1995 ◽

Vol 30 (4) ◽

pp. 852D-852

Author(s):

Sabrina L. Shaw ◽

William F. Hayslett ◽

Eddie B. Williams

Keyword(s):

Plant Growth ◽

Plant Height ◽

Plant Growth Regulators ◽

Growth Regulators ◽

Dry Weight ◽

Fresh Weight ◽

Visual Appearance ◽

Top Dry Weight ◽

Viola Tricolor

A one-time application of fish emulsion 2 days before the application of plant growth regulators (PGR) showed an overriding effect on the growth of pansies. Blue/blotch shades of `Medallion' pansies were placed on a constant feed program of 100 ppm Peat Lite 20N–10P–20K, with half of the pansies receiving an additional one-time supplement of fish emulsion. PGRs and rates included B-Nine, 0.5% (used as the control); uniconazole, 2 and 4 ppm; and paclobutrazol, 16 and 25 ppm. Parameters taken included plant height, top fresh weight, top dry weight, days to anthesis, and visual appearance. Significant differences were noted in the plants receiving the supplement for plant dry weight, plant height, and visual appearance. Plants receiving fish emulsion grew taller and denser than those on constant feed alone despite the effects of the PGRs.

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THE ROLL OF SOME PLANT GROWTH REGULATORS ON SHOOTS MULTIPLICATION OF STEVIA PLANTS IN VITRO

IRAQI JOURNAL OF AGRICULTURAL SCIENCES ◽

10.36103/ijas.v48i5.322 ◽

2017 ◽

Vol 48 (5) ◽

Author(s):

Al-Obaidy & Khierallah

Keyword(s):

Plant Growth ◽

Plant Growth Regulators ◽

Growth Regulators ◽

Culture Media ◽

Stevia Rebaudiana ◽

Shoot Multiplication ◽

Dry Weight ◽

Fresh Weight ◽

Number Of Shoots

This research was conducted to study the effect of some plant growth regulators on in vitro shoots multiplication of stevia (Stevia rebaudiana Bertoni). The experiments included tests of various combinations of KIN with IBA or IAA in the shoot multiplication. Results indicated that KIN at 1.0 mg. L-1 plus 0.3 mg. L-1 of IBA produced the highest number of shoots (3.5 shoots) while KIN at 1.5 mg. L-1 plus IBA at 1.0 mg. L-1 produced the lowest shoot length (1.14 cm). Hormone free medium produced the highest rate of the leaves number reached 28.56 leaves. KIN and IBA interaction increased fresh and dry weight significantly. Treatment contained 2.0 mg -1 KIN plus 0.3 mg. L-1 IBA produced the highest fresh weight (1.739 g) while 0.5 mg. L-1 KIN and 0.3 mg. L-1 IBA produced the highest dry weight (0.822 g). As for the effect of interaction between the IAA and KIN it was significant in the number of shoots formed. Interaction between 1.0 mg. L-1 KIN with 0.1 mg. L-1IAA produced the highest number of shoots (3.8 shoots). Shoots length reached 8.10 cm in the media with 0.3 mg. L-1 IAA only. The highest fresh weight (1.267 g) was achieved with the interaction between 1.0 mg. L-1 KIN and 0.3 mg. L-1 IAA while 0.5 mg. L-1IAA without KIN produced the highest dry weight reached 0.138 g. Shoots multiplication was improved by incorporation of the cytokinin TDZ in culture media. Shoots number, fresh and dry weights were increased significantly by adding 0.05 mg. L-1 of TDZ at present of 0.3 mg. L-1 of IBA giving 6.6 shoots, 0.974 g and 0.144 g respectively while shoots length decreased significantly as media without TDZ produced the highest shoots length reached 9.32 cm. The above results can adopt for the successful in vitro shoot multiplication of Stevia plants.

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Influencing Stock Production of Mojave Sage and Cape Daisy with the Application of Plant Growth Regulators

HortTechnology ◽

10.21273/horttech04736-20 ◽

2021 ◽

pp. 1-7

Author(s):

Sean J. Markovic ◽

James E. Klett

Keyword(s):

Plant Growth ◽

Plant Growth Regulators ◽

Growth Regulators ◽

Growth Index ◽

Dry Weight ◽

The Other ◽

Plant Production ◽

Stock Plant ◽

Fresh Weight ◽

Vegetative Cuttings

This study aimed to assess the effects of plant growth regulators (PGRs) on stock plant production of mojave sage (Salvia pachyphylla) and ‘Avalanche’ cape daisy (Osteospermum hybrid) that received foliar sprays of the following three PGRs: 200 and 400 ppm ethephon; 250 and 500 ppm benzyladenine; and 50 and 100 ppm gibberellic acid 4 and 7 (GA4+7) plus benzyladenine. Vegetative growth [height and width growth index (GI)], the number of vegetative cuttings, and fresh weight (FW) and dry weight (DW) of the harvested vegetative cuttings data were collected. A propagation study was conducted concurrently to determine the effects of the PGR treatments on rooting vegetative cuttings. GA4+7 plus benzyladenine (50 and 100 ppm) increased the production of both mojave sage and ‘Avalanche’ cape daisy cuttings by ≥18% more than the other treatments. The GI, FW, and DW results showed similar trends across experiments 1 and 2 for each perennial. In the propagation study, the rooting percentage did not differ after 4 weeks, indicating that the use of GA4+7 plus benzyladenine in production protocols could benefit producers of both perennials.

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Effects of plant growth regulators on the growth and lipid accumulation in the solid endosperm of Ta Xanh coconut (Cocos nucifera L.)

Science and Technology Development Journal ◽

10.32508/stdj.v19i3.467 ◽

2016 ◽

Vol 19 (3) ◽

pp. 43-51

Author(s):

Bup Kim Nguyen ◽

Tien Thi Thuy Le ◽

Viet Trang Bui

Keyword(s):

Plant Growth ◽

Plant Growth Regulators ◽

Growth Regulators ◽

Lipid Accumulation ◽

Cocos Nucifera ◽

Dry Weight ◽

Fresh Weight ◽

Ripening Stages ◽

Cocos Nucifera L ◽

Positive Effect

The growth of Ta Xanh coconut fruit is divided into four stages: slow growth, rapid growth, mature and ripening. In the mature and ripening stages, lipid accumulation increased at the strongest level in the endosperm. This study was conducted with the purpose of studying the effects of plant growth regulators on the growth and lipid accumulation in copra (solid endosperm) from the mature to ripening stage in Ta Xanh coconut fruit. Fresh weight, dry weight and lipid content in solid endosperm after treatments with 1 mg/L NAA, 10 mg/L BA and 20 mg/L GA3 individually or in combination with ethrel were determined. Results showed that the treatment of 20 mg/L GA3 on the 8-month-old fruits increased the copra fresh weight and dry weight. Treatments of 1 mg/L NAA, 20 mg/l GA3 or 200-250 mg/L ethrel on the 10-month-old fruits increased the ratio of lipid in the copra of each fruit. Treatment of 200 mg/L ethrel had a positive effect on increasing of the fresh weight, dry weight and lipid concent in the solid endosperm of the 10-month-old Ta Xanh coconut after 1 or 2 weeks of combinated treatment of 1 mg/L NAA, 10 mg/L BA and 20 mg/L GA3.

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Sterilization protocols and the effect of plant growth regulators on callus induction and secondary metabolites production in in vitro cultures Melia azedarach L.

AMB Express ◽

10.1186/s13568-022-01343-8 ◽

2022 ◽

Vol 12 (1) ◽

Author(s):

Fatemeh Ahmadpoor ◽

Nasser Zare ◽

Rasool Asghari ◽

Parisa Sheikhzadeh

Keyword(s):

Secondary Metabolites ◽

Plant Growth ◽

Plant Growth Regulators ◽

Growth Regulators ◽

Callus Induction ◽

Callus Cultures ◽

Melia Azedarach ◽

Ms Medium ◽

Fresh Weight ◽

Metabolites Production

AbstractMelia azedarach L. is a valuable source of antioxidants and secondary metabolites. This study is a first extensive report about the effect of different serialization protocols and plant growth regulators (PGRs) on explant disinfection efficiency, callus induction and secondary metabolites production and accumulation in callus cultures of M. azedarach L. In this regard, the effect of plant growth regulators on callus induction and secondary metabolites production were examined. In addition, different sterilization agents were evaluated for disinfection of chinaberry leaf explants. The results showed that the lowest percentage of explant contamination and browning with the highest percentage of callus induction and callus growth obtained with explants pretreated with benomyl (2 g/L) for 2 h and sterilized with 7% H2O2 for 10 min and NaOCl 2% (without pH adjustment) for 12 min. Although adjusting the pH of NaOCl to pH = 7 and 10 significantly reduced the microbial contamination and increased the percentage of contamination-free cultures of M. azedarach L., adversely influenced the explant viability and callus induction and growth. The highest percentage of callus induction obtained on the MS medium containing 3 mg/L NAA/2,4-D and 1 or 3 mg/L Kin/BAP, and the highest callus yield (1804.833 mg/explant) belonged to the MS medium supplemented with 5 mg/L 2,4-D and 5 mg/L Kin. The callus cultures grown on the MS medium supplemented with 3 mg/L NAA and 1 mg/L Kin produced the highest amount of Quercetin (2.06 mg/g fresh weight), Rutin (5.56 mg/g fresh weight) and Kaempferol (1.84 mg/g fresh weight).

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Increasing Stock Production of Two Herbaceous Perennials with the Application of Plant Growth Regulators

HortTechnology ◽

10.21273/horttech04492-19 ◽

2020 ◽

Vol 30 (3) ◽

pp. 421-427

Author(s):

Sean J. Markovic ◽

James E. Klett

Keyword(s):

Plant Growth ◽

Plant Growth Regulators ◽

Growth Regulators ◽

Growth Index ◽

Dry Weight ◽

Stock Plant ◽

Fresh Weight ◽

Herbaceous Perennials ◽

Significant Difference ◽

Vegetative Cuttings

The objective of these experiments was to evaluate the reaction of ‘Snow Angel’ coral bells (Heuchera sanguinea) and Orange Carpet hummingbird trumpet (Epilobium canum ssp. garrettii ‘PWWG01S’) to repeated foliar applications of three plant growth regulators at two application rates. The plant growth regulators applied during a stock plant study and followed by a propagation study were 200 and 400 ppm ethephon, 250 and 500 ppm benzyladenine, and 50 and 100 ppm gibberellic acid 4 and 7 (GA4+7) + benzyladenine. The stock plant study was conducted to assess the efficacy of plant growth regulators, vegetative growth (height and width growth index), the number of vegetative cuttings, as well as the fresh weight (FW) and dry weight (DW) of the harvested vegetative cuttings. The propagation study was conducted to determine the effects of the plant growth regulator treatments on the rooting of the vegetative cuttings. The stock plant study showed that GA4+7 + benzyladenine (50 and 100 ppm) significantly increased production of ‘Snow Angel’ coral bells cuttings compared with all other treatments. However, no significant differences in FW or DW were observed with ‘Snow Angel’ coral bells between treatments. In the propagation study, no significant difference in rooting percentage was observed after 4 weeks. The Orange Carpet hummingbird trumpet stock plant study resulted in a greater number of vegetative cuttings with GA4+7 + benzyladenine (50 and 100 ppm) and benzyladenine (250 ppm) treatments. Fresh weight of vegetative cuttings harvested from plants treated with GA4+7 + benzyladenine (50 or 100 ppm) were the lowest. The only treatment that showed increased vegetative cutting production with no effect on FW was benzyladenine (250 ppm) on Orange Carpet hummingbird trumpet.

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The Effects of Plant Growth Regulators On The Oil Content And Fatty Acid Composition of Ricinus Communis L.: An In Vitro Study

10.21203/rs.3.rs-724779/v1 ◽

2021 ◽

Author(s):

Zeynep Ergun

Keyword(s):

Fatty Acid Composition ◽

Fatty Acid ◽

Plant Growth ◽

Plant Growth Regulators ◽

Acid Composition ◽

Growth Regulators ◽

Ricinus Communis ◽

Fatty Acid Profiles ◽

The Media

Abstract This study aimed to investigate the effects of plant growth regulators in callus cell culture on the oil content and fatty acid composition of the castor bean (Ricinus communis L.). Ricinus communis L. are valued for its oil and the performance of oil is closely related to its fatty acid composition. Thus, producing oil in vitro with favored fatty acid profiles is a promising research area and may also offer industrial opportunities. In line with this, the total amount of fat and the fatty acid composition of the samples, which were endosperm and calli obtained by treatment of various doses of plant growth regulators were determined. Results showed that the type and amount of the plant growth regulator used in the media affect the fatty acid composition. In detail, the biggest change was shown by Indole-3-Acetic Acid (IAA), in general, using the plant growth regulators at 5 mgL-1, instead of 20 mgL-1, was found to have induced larger differentiations. The effect of a natural plant growth regulator (IAA) on fatty acid profiles was larger than the synthetic ones (NAA, 1-Naphthaleneacetic acid, and 2,4 D, 2,4-Dichlorophenoxyacetic acid). The media containing 5 mgL-1 of NAA, 20 mgL-1 of NAA, 20 mgL-1 of 2,4 D, or 5 mgL-1 of 2,4 D gave similar results.

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