scholarly journals Influencing Stock Production of Mojave Sage and Cape Daisy with the Application of Plant Growth Regulators

2021 ◽  
pp. 1-7
Author(s):  
Sean J. Markovic ◽  
James E. Klett
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Growth Index ◽  
Dry Weight ◽  
The Other ◽  
Plant Production ◽  
Stock Plant ◽  
Fresh Weight ◽  
Vegetative Cuttings

This study aimed to assess the effects of plant growth regulators (PGRs) on stock plant production of mojave sage (Salvia pachyphylla) and ‘Avalanche’ cape daisy (Osteospermum hybrid) that received foliar sprays of the following three PGRs: 200 and 400 ppm ethephon; 250 and 500 ppm benzyladenine; and 50 and 100 ppm gibberellic acid 4 and 7 (GA4+7) plus benzyladenine. Vegetative growth [height and width growth index (GI)], the number of vegetative cuttings, and fresh weight (FW) and dry weight (DW) of the harvested vegetative cuttings data were collected. A propagation study was conducted concurrently to determine the effects of the PGR treatments on rooting vegetative cuttings. GA4+7 plus benzyladenine (50 and 100 ppm) increased the production of both mojave sage and ‘Avalanche’ cape daisy cuttings by ≥18% more than the other treatments. The GI, FW, and DW results showed similar trends across experiments 1 and 2 for each perennial. In the propagation study, the rooting percentage did not differ after 4 weeks, indicating that the use of GA4+7 plus benzyladenine in production protocols could benefit producers of both perennials.

scholarly journals Increasing Stock Production of Two Herbaceous Perennials with the Application of Plant Growth Regulators

2020 ◽  
Vol 30 (3) ◽  
pp. 421-427
Author(s):  
Sean J. Markovic ◽  
James E. Klett
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Growth Index ◽  
Dry Weight ◽  
Stock Plant ◽  
Fresh Weight ◽  
Herbaceous Perennials ◽  
Significant Difference ◽  
Vegetative Cuttings

The objective of these experiments was to evaluate the reaction of ‘Snow Angel’ coral bells (Heuchera sanguinea) and Orange Carpet hummingbird trumpet (Epilobium canum ssp. garrettii ‘PWWG01S’) to repeated foliar applications of three plant growth regulators at two application rates. The plant growth regulators applied during a stock plant study and followed by a propagation study were 200 and 400 ppm ethephon, 250 and 500 ppm benzyladenine, and 50 and 100 ppm gibberellic acid 4 and 7 (GA4+7) + benzyladenine. The stock plant study was conducted to assess the efficacy of plant growth regulators, vegetative growth (height and width growth index), the number of vegetative cuttings, as well as the fresh weight (FW) and dry weight (DW) of the harvested vegetative cuttings. The propagation study was conducted to determine the effects of the plant growth regulator treatments on the rooting of the vegetative cuttings. The stock plant study showed that GA4+7 + benzyladenine (50 and 100 ppm) significantly increased production of ‘Snow Angel’ coral bells cuttings compared with all other treatments. However, no significant differences in FW or DW were observed with ‘Snow Angel’ coral bells between treatments. In the propagation study, no significant difference in rooting percentage was observed after 4 weeks. The Orange Carpet hummingbird trumpet stock plant study resulted in a greater number of vegetative cuttings with GA4+7 + benzyladenine (50 and 100 ppm) and benzyladenine (250 ppm) treatments. Fresh weight of vegetative cuttings harvested from plants treated with GA4+7 + benzyladenine (50 or 100 ppm) were the lowest. The only treatment that showed increased vegetative cutting production with no effect on FW was benzyladenine (250 ppm) on Orange Carpet hummingbird trumpet.

scholarly journals Plant Growth Regulator Impacts on Vegetative Cutting Production of Moroccan Pincushion (Pterocephalus depressus) Plants

2021 ◽  
Vol 39 (2) ◽  
pp. 62-67
Author(s):  
Sean J. Markovic ◽  
James E. Klett
Keyword(s):  
Plant Growth ◽  
Gibberellic Acid ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Growth Regulator ◽  
Plant Growth Regulator ◽  
Growth Index ◽  
Dry Weight ◽  
Stock Plant ◽  
Cutting Production

Abstract Moroccan pincushion (Pterocephalus depressus) is a drought-tolerant perennial that is being used in landscapes throughout arid areas of the western United States. This paper describes two experiments researching vegetative cutting production from stock plants. Moroccan pincushion stock plants received foliar applications of gibberellic acid (GA3), benzyladenine, ethephon, or auxin [indole-3-butyric acid (IBA)] plant growth regulators (PGR). Plant growth regulators were applied singularly and in combination with GA3 to determine efficacy on stock plant growth. A propagation study was conducted simultaneously to determine effects of these different PGR treatments applied to stock plants on the rooting of moroccan pincushion cuttings. The stock plant study showed GA3 + benzyladenine application increased cutting production over other PGR treatments. Fresh weight of moroccan pincushion cuttings did not differ among treatments. While cuttings did not differ in dry weight in experiment 1, statistical differences were observed in experiment 2. However, these differences in dry weight did not affect the quality of the cuttings. Cuttings from stock plants treated with GA3 + IBA treatment had the highest numerical growth index [(height + width + width)/3]. Cuttings from stock plants treated with GA3 alone or in combination with another PGR were all greater in average growth index and statistically differed from those without GA3 being applied. PGR treatments did not affect rooting percentages of the cuttings with nontreated stock plant cuttings successfully rooting at an average rate of 95%. However, GA3 + IBA was the only treatment where cuttings had 100% rooting for both experiments, indicating potential rooting benefits. Index words: Plant growth regulator, propagation, Pterocephalus depressus, vegetative cuttings. Species used in this study: Moroccan pincushion [Pterocephalus depressus Archibald]. Chemicals used in this study: gibberellic acid (GA3), benzyladenine, ethephon, indole-3-butyric acid (IBA).

scholarly journals Susceptibility in the Growth of Viola tricolor `Blue' to Plant Growth Regulators and Fish Emulsion

HortScience ◽  
1995 ◽  
Vol 30 (4) ◽  
pp. 852D-852
Author(s):  
Sabrina L. Shaw ◽  
William F. Hayslett ◽  
Eddie B. Williams
Keyword(s):  
Plant Growth ◽  
Plant Height ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Dry Weight ◽  
Fresh Weight ◽  
Visual Appearance ◽  
Top Dry Weight ◽  
Viola Tricolor

A one-time application of fish emulsion 2 days before the application of plant growth regulators (PGR) showed an overriding effect on the growth of pansies. Blue/blotch shades of `Medallion' pansies were placed on a constant feed program of 100 ppm Peat Lite 20N–10P–20K, with half of the pansies receiving an additional one-time supplement of fish emulsion. PGRs and rates included B-Nine, 0.5% (used as the control); uniconazole, 2 and 4 ppm; and paclobutrazol, 16 and 25 ppm. Parameters taken included plant height, top fresh weight, top dry weight, days to anthesis, and visual appearance. Significant differences were noted in the plants receiving the supplement for plant dry weight, plant height, and visual appearance. Plants receiving fish emulsion grew taller and denser than those on constant feed alone despite the effects of the PGRs.

scholarly journals THE ROLL OF SOME PLANT GROWTH REGULATORS ON SHOOTS MULTIPLICATION OF STEVIA PLANTS IN VITRO

2017 ◽  
Vol 48 (5) ◽  
Author(s):  
Al-Obaidy & Khierallah
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Culture Media ◽  
Stevia Rebaudiana ◽  
Shoot Multiplication ◽  
Dry Weight ◽  
Fresh Weight ◽  
Number Of Shoots

This research was conducted to study the effect of some plant growth regulators on in vitro shoots multiplication of stevia (Stevia rebaudiana Bertoni). The experiments included tests of various combinations of KIN with IBA or IAA in the shoot multiplication. Results indicated that KIN at 1.0 mg. L-1 plus 0.3 mg. L-1 of IBA produced the highest number of shoots (3.5 shoots) while KIN at 1.5 mg. L-1 plus IBA at 1.0 mg. L-1 produced the lowest shoot length (1.14 cm).  Hormone free medium produced the highest rate of the leaves number reached 28.56 leaves. KIN and IBA interaction increased fresh and dry weight significantly.   Treatment contained 2.0 mg -1 KIN plus 0.3 mg. L-1 IBA produced the highest fresh weight (1.739 g) while 0.5 mg. L-1 KIN and 0.3 mg. L-1 IBA produced the highest dry weight (0.822 g). As for the effect of interaction between the IAA and KIN it was significant in the number of shoots formed. Interaction between 1.0 mg. L-1 KIN with 0.1 mg. L-1IAA produced the highest number of shoots (3.8 shoots). Shoots length reached 8.10 cm in the media with 0.3 mg. L-1 IAA only. The highest fresh weight (1.267 g) was achieved with the interaction between 1.0 mg. L-1 KIN and 0.3 mg. L-1 IAA while 0.5 mg. L-1IAA without KIN produced the highest dry weight reached 0.138 g.  Shoots multiplication was improved by incorporation of the cytokinin TDZ in culture media. Shoots number, fresh and dry weights were increased significantly by adding 0.05 mg. L-1 of TDZ at present of 0.3 mg. L-1 of IBA giving 6.6 shoots, 0.974 g and 0.144 g respectively while shoots length decreased significantly as media without TDZ produced the highest shoots length reached 9.32 cm. The above results can adopt for the successful in vitro shoot multiplication of Stevia plants. 

scholarly journals Callus Induction of Gendarussa (Justicia gendarussa) by Various Concentration of 2,4-D, IBA, and BAP

2017 ◽  
Vol 9 (3) ◽  
pp. 402 ◽  
Author(s):  
Dwi Kusuma Wahyuni ◽  
Putri Andriani ◽  
Arif Nur Muhammad Ansori ◽  
Edy Setiti Wida Utami
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Callus Induction ◽  
Callus Formation ◽  
Leaf Explants ◽  
Culture Method ◽  
Dry Weight ◽  
Fresh Weight ◽  
Two Factors

<p class="IsiAbstrakIndo"><em>Justicia gendarussa </em>Burm.f., a medicinal plant, is Acanthaceae that has many functions. Furthermore, the compounds in gendarussa must be produced in high quantity and quality by applying callus culture method. Accordingly, it is important to study the effects of plant growth regulators (2,4-D, IBA, and BAP) on callus induction of gendarussa leaves. This research design utilized a factorial design with two factors (2,4-D and IBA: 0.5, 1, 1.5 mg/L and BAP: 0.5, 1, 1.5, 2 mg/L). The experiment consisted of 24 treatments, each of which was repeated 3 times. Observation was carried out in 6 weeks. Data on the time of callus formation, percentage of explants formed callus, and callus morphology were analyzed descriptively,while data on fresh and dry weight were analyzed by Two-Way ANOVA (<span>α</span> = 0.5). Interestingly, the results showed that various concentration of plant growth regulators (2,4-D, IBA, and BAP) affected callus induction from leaf explants of gendarussa. We concluded that the most optimal treatment combination of concentration of plant growth regulators in inducing callus from leaf explants of gendarussa is 1.5 mg/L 2,4-D and 2 mg/L BAP with a relatively long period of callus formation at the earliest, i.e. on day 5, 2.247 g of fresh weight, 0.108 gof dry weight, white callus translucent, and friable. <span lang="EN-GB">Moreover, t</span>he optimum treatment will be used to produce secondary metabolite and seed s<span lang="EN-GB">y</span>nthetic by cell suspension culture.</p>

scholarly journals Effects of plant growth regulators on the growth and lipid accumulation in the solid endosperm of Ta Xanh coconut (Cocos nucifera L.)

2016 ◽  
Vol 19 (3) ◽  
pp. 43-51
Author(s):  
Bup Kim Nguyen ◽  
Tien Thi Thuy Le ◽  
Viet Trang Bui
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Lipid Accumulation ◽  
Cocos Nucifera ◽  
Dry Weight ◽  
Fresh Weight ◽  
Ripening Stages ◽  
Cocos Nucifera L ◽  
Positive Effect

The growth of Ta Xanh coconut fruit is divided into four stages: slow growth, rapid growth, mature and ripening. In the mature and ripening stages, lipid accumulation increased at the strongest level in the endosperm. This study was conducted with the purpose of studying the effects of plant growth regulators on the growth and lipid accumulation in copra (solid endosperm) from the mature to ripening stage in Ta Xanh coconut fruit. Fresh weight, dry weight and lipid content in solid endosperm after treatments with 1 mg/L NAA, 10 mg/L BA and 20 mg/L GA3 individually or in combination with ethrel were determined. Results showed that the treatment of 20 mg/L GA3 on the 8-month-old fruits increased the copra fresh weight and dry weight. Treatments of 1 mg/L NAA, 20 mg/l GA3 or 200-250 mg/L ethrel on the 10-month-old fruits increased the ratio of lipid in the copra of each fruit. Treatment of 200 mg/L ethrel had a positive effect on increasing of the fresh weight, dry weight and lipid concent in the solid endosperm of the 10-month-old Ta Xanh coconut after 1 or 2 weeks of combinated treatment of 1 mg/L NAA, 10 mg/L BA and 20 mg/L GA3.

scholarly journals 031 EFFECT OF BLANCHING, IBA CONCENTRATION, AND PLANT GROWTH REGULATORS ON GROWTH AND OVERWINTER SURVIVAL OF CUTTING-PROPAGATED ORNAMENTALS

HortScience ◽  
1994 ◽  
Vol 29 (5) ◽  
pp. 432b-432
Author(s):  
Anna Perkins Nina Bassuk
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Foliar Spray ◽  
Acer Rubrum ◽  
The Other ◽  
Spray Application ◽  
Stock Plant ◽  
Overwinter Survival ◽  
Silver Thiosulfate

Budbreak inhibition and poor overwinter survival (OS) limit successful cutting propagation of Acer rubrum October Glory, A. rubrum Red Sunset, Hamamelis vernalis, H. virginiana and Stewartia pseudocamellia. Localized blanching (banding) of the cutting on the stock plant; a range of 3 IBA concentrations, and foliar spray application of: 1% silver thiosulfate(STS), STS followed ten days later by Gibberellin, GA4/7:250ppm(STS GA),50ppm thidiazuron (TDZ) and TDZ followed by GA4/7 (TDZ GA)were tested for increasing growth and overwinter survival.. Carbohydrates were analyzed in cuttings which did and didn't grow. A. rubrum October Glory*, and Hamamelis spp all had increased OS for cuttings which grew. A. rubrum Red Sunset demonstrated a similar trend. Hamamelis spp. had significant increase in carbohydrates for cuttings which grew. A. rubrum October Glory' exhibited the same trend. S. pseudocamellia did not have increased OS with growth. and showed no increases in carbohydrates with growth, but the cuttings that didn't grow had at least 93 % more carbohydrates than the other species analyzed. All species had higher OS when stored in the 3° C cooler, than in the fluctuating cold frame. Banding increased growth of A. rubrum October Glory, and H. virginiana. IBA concentration affected growth of all species. STS increased growth of H. virginiana and S. pseudocamellia. GA4/7 increased growth of all cuttings except A. rubrum October Glory.

scholarly journals Effect of plant growth regulators on callus induction and Rutin production of Ricinus communis L. plant

2017 ◽  
Vol 14 (3) ◽  
pp. 461-468
Author(s):  
Baghdad Science Journal
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Callus Induction ◽  
Ricinus Communis ◽  
Dry Weight ◽  
Fresh Weight ◽  
Ricinus Communis L ◽  
Medical Plant ◽  
The Media

Ricinus communis L. is an important medical plant hence it contains many active compounds. The aim of this research is to study the effect of plant growth regulators on callus induction and Rutin concentration. A combination of Benzyle adenine (BA) and Indol Acetic acid (IAA) at (0.0,1.0,2.0) mg/L was added to the media, the highest fresh weight of the induced callus from stem explant was (4.97) gr . at (1.0,1.0) mg/L BA and IAA consenquently the same combination gave the highest dry weight of callus (0.42) gr. while the combination at (2.0,1.0) mg/L BA and IAA gave the highest fresh weight of induced callus from Leaves explant (5.28) gr., then (2.0,1.0) mg/L BA and IAA gave the highest dry weight for callus induced from leaves at (0.55)gr.Spectrophotometer used to estimate rutin quantity and results showed that the present of rutin at (126.31) ppm in callus induced from stem at (2.0,2.0) mg/L BA and IAA, the highest value of this compound (121.05) ppm on callus induction from the leaves in the same combination as compared with Rutin quantity in intact plant that reached (94.73) ppm in leaves and (68.42) ppm in stem.

scholarly journals Application of Plant Extracts in Micropropagation and Cryopreservation of Bleeding Heart: An Ornamental-Medicinal Plant Species

Agriculture ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 542
Author(s):  
Dariusz Kulus ◽  
Natalia Miler
Keyword(s):  
Tissue Culture ◽  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Plant Extracts ◽  
The Other ◽  
White Gold ◽  
Natural Extracts ◽  
Other Hand ◽  
The Impact

Lamprocapnos spectabilis (L.) Fukuhara (bleeding heart) is valued both in the horticultural and pharmaceutical markets. Despite its great popularity, information on the in vitro tissue culture technology in this species is limited. There is also little knowledge on the application of plant extracts in the tissue culture systems of plants other than orchids. The aim of this study is to compare the utility of traditional plant growth regulators (PGRs) and natural extracts—obtained from the coconut shreds, as well as oat, rice, and sesame seeds—in the micropropagation and cryopreservation of L. spectabilis ‘Gold Heart’ and ‘White Gold’. The biochemical analysis of extracts composition is also included. In the first experiment related to micropropagation via axillary buds activation, the single-node explants were cultured for a 10-week-long propagation cycle in the modified Murashige and Skoog medium fortified either with 1.11 µM benzyladenine (BA) and 1.23 µM indole-3-butritic acid (IBA) or with 10% (v/v) plant extracts. A PGRs- and extract-free control was also considered. In the cryopreservation experiment, the same 10% (v/v) extracts were added into the medium during a seven-day preculture in the encapsulation-vitrification cryopreservation protocol. It was found that the impact of natural additives was cultivar- and trait-specific. In the first experiment, the addition of coconut extract favoured the proliferation of shoots and propagation ratio in bleeding heart ‘Gold Heart’. Rice extract, on the other hand, promoted callus formation in ‘White Gold’ cultivar and was more effective in increasing the propagation ratio in this cultivar than the conventional plant growth regulators (4.1 and 2.6, respectively). Sesame extract suppressed the development of the explants in both cultivars analysed, probably due to the high content of polyphenols. As for the second experiment, the addition of plant extracts into the preculture medium did not increase the survival level of the cryopreserved shoot tips (sesame and oat extracts even decreased this parameter). On the other hand, coconut extract, abundant in simple sugars and endogenous cytokinins, stimulated a more intensive proliferation and growth of shoots after rewarming of samples. Analysing the synergistic effect of conventional plant growth regulators and natural extracts should be considered in future studies related to L. spectabilis.

Sign in / Sign up

Export Citation Format

Share Document