scholarly journals Evaluation of cellular immune response in Golden Hamsters experimentally infected with Leishmania donovani comparing with cellular immune response against chicken Red Blood Cells.

2012 ◽  
Vol 9 (1) ◽  
pp. 23-30
Author(s):  
Baghdad Science Journal
Keyword(s):  
Immune Response ◽  
Red Blood Cells ◽  
Skin Test ◽  
Blood Cells ◽  
Cellular Immune Response ◽  
Leishmania Donovani ◽  
Cellular Immune ◽  
And Control ◽  
Delayed Type Of Hypersensitivity

The Evaluation of the immune response in Golden Hamsters experimentally infected with Leishmania donovani was determined in this study, particularly, the cellular immune response. Follow up has maintained to determine the Delayed Type of Hypersensitivity using skin test both in infected and control lab animals. Chicken red blood cells were used as a parameter to evaluate the immune system; they are dull and have the ability of immunization. Two concentrations of chicken R.B.C were examined to determine which gives the higher titration in Hamsters and those were 1.5 X 109 cell/ml and 3 X 109 cell/ml , the second concentration gave the maximum titration where then used in this work. After sensitization with Chicken R.B.C for both infected and control groups, delayed type of hypersensitivity has been used against Leishmania donovani antigen and 4 days of follow up were adopted and they were (14, 30, 60, 90) day after infection. Results showed that skin test against both antigens ( L.donovani and chicken R.B.C) was significantly higher than normal at the first day of follow up ( day 14) then gradual decreasing were noticed till the last day of follow up (90). This can indicate that the infection with L.donovani activated the immune response at the beginning of infection, then leads to cellular immune suppression against both L.donovani antigen and chicken R.B.C., so that this immunosuppression is not specific.

scholarly journals Evaluation of Proteus vulgarisfimbriae antigen by delayed type hypersensitivity (DTH)-skin test in rabbits

2011 ◽  
Vol 35 (1) ◽  
pp. 100-106
Author(s):  
Ekram Abbas Abood Al-Samarrae
Keyword(s):  
Immune Response ◽  
Skin Test ◽  
Cellular Immune Response ◽  
Control Site ◽  
Delayed Type Hypersensitivity ◽  
Proteus Vulgaris ◽  
Significant Difference ◽  
Cellular Immune ◽  
And Control

The aim of this study is to evaluate the use of fimbriae antigens for immunization of rabbits against Proteus vulgaris bacteria results showed a higher significant difference (P<0.05) in erythema diameters in the immunized groups in compared with the control. There was no significant difference between both immunized groups 200 μg/ml and 100 μg/ml after 24 hours. Also, a higher significant differences (P<0.05) in the diameter of induration is recorded in both immunized groups compared to control site, a higher significant differences (P<0.05) in the immunized group (100 μg/ml) at the concentration 200 μg/ml compared with 100 and 50 μg/ml after 24 hours, as follow as after 48 hours except significant differences between 200 and 100 μg/ml concentration (P<0.01) and increase induration after 72 hours between both immunized groups; within groups and control site. Conclusion that the fimbrial antigen have the ability to elicit cellular immune response by delayed type hypersensitivity (DTH).

Assessment of different diagnostic methods for scabies with follow-up of cellular immune response

2015 ◽  
Vol 0 (0) ◽  
pp. 0
Author(s):  
Salwa El-Din ◽  
AmiraM Matar ◽  
NashaatE Nassef ◽  
NadiaS El-Nahas
Keyword(s):  
Immune Response ◽  
Cellular Immune Response ◽  
Diagnostic Methods ◽  
Cellular Immune

Cellular immune response is not associated with incident cancer or total mortality: a prospective follow-up

2006 ◽  
Vol 15 (6) ◽  
pp. 548-550 ◽  
Author(s):  
Mark J. Roth ◽  
You-Lin Qiao ◽  
Christian C. Abnet ◽  
You-Hui Zhang ◽  
Sanford M. Dawsey ◽  
...  
Keyword(s):  
Immune Response ◽  
Cellular Immune Response ◽  
Total Mortality ◽  
Incident Cancer ◽  
Cellular Immune

Cellular immune response of Drosophila melanogaster against Asobara tabida

Parasitology ◽  
1981 ◽  
Vol 83 (2) ◽  
pp. 319-324 ◽  
Author(s):  
A. J. Nappi
Keyword(s):  
Immune Response ◽  
Blood Cell ◽  
Blood Cells ◽  
Cellular Immune Response ◽  
Cell Contact ◽  
Hormonal Changes ◽  
Capsule Formation ◽  
Asobara Tabida ◽  
Cellular Immune ◽  
Crystal Cells

SUMMARYThe successful cellular immune response of larvae of D. melanogaster against A. tabida is characterized by an increase in the number of blood cells, a premature transformation of spherical plasmatocytes to flattened, disc-shaped lamellocytes, and the lysis of crystal cells. The plasmatocytes and transformed lamellocytes rapidly aggregate around the eggs of the parasite to form a cellular capsule, while the lysing crystal cells release substances causing the melanization of the capsule. The early appearance of large numbers of transformed blood cells in the haemolymph prior to their involvement in capsule formation suggests that transformation is independent of cell contact with the parasite and may be due instead to biochemical stimuli emitted from the parasite and/or by hormonal changes. Quantitative blood cell studies of hosts exhibiting no encapsulation and melanization response indicate that successfully developing parasites are recognized by the immune system but are able sufficiently to block the blood cell transformation and thus prevent capsule formation.

Leishmania donovani: Evolution and Architecture of the Splenic Cellular Immune Response Related to Control of Infection

2001 ◽  
Vol 99 (1) ◽  
pp. 17-25 ◽  
Author(s):  
Peter C. Melby ◽  
Adriana Tabares ◽  
Blanca I. Restrepo ◽  
Astrid E. Cardona ◽  
H.Stan McGuff ◽  
...  
Keyword(s):  
Immune Response ◽  
Cellular Immune Response ◽  
Leishmania Donovani ◽  
Cellular Immune

scholarly journals Reduced schedule of human anti-rabies immunization with Fuenzalida & Palacios vaccine: additional data

1993 ◽  
Vol 35 (3) ◽  
pp. 281-284 ◽  
Author(s):  
S.R. Favoretto ◽  
M.L. Carrieri ◽  
M.S. Tino ◽  
A. Assis ◽  
C.R. Zanetti ◽  
...  
Keyword(s):  
Immune Response ◽  
Skin Test ◽  
Cellular Immune Response ◽  
Nervous Tissue ◽  
Additional Data ◽  
Cell Mediated Immunity ◽  
Newborn Mice ◽  
Serum Neutralization ◽  
Cellular Immune ◽  
Antibody Levels

It was reevaluated a reduced schedule for anti-rabies post-exposure immunization with newborn mice nervous tissue vaccine (Fuenzalida 8c Palacios) in a group of 30 non exposed volunteers. The vaccine was administered by intramuscular injections on days zero, 2, 4, 16 and 27, in the deltoid area. Antibody levels were determinated by a simplified serum neutralization microtest on days zero, 16 and 37. On days 16 and 37 the antibody levels of the whole group was >0.5 IU/ml and >1.0 IU/ml, respectively. The cell mediated immunity was precociously detected (on day 4) by the delayed type hipersensitivity skin test. Our results show that this reduced schedule elicited an early and effective humoral and cellular immune response. However it is necessary other studies with larger groups of vaccinees in order to obtain definitive conclusion.

scholarly journals Induction of Cellular Immune Response by DNA Vaccine CoexpressingE. acervulina3-1E Gene and Mature CHIl-15 Gene

2012 ◽  
Vol 2012 ◽  
pp. 1-7
Author(s):  
Dexing Ma ◽  
Chunli Ma ◽  
Mingyang Gao ◽  
Guangxing Li ◽  
Ze Niu ◽  
...  
Keyword(s):  
Immune Response ◽  
Dna Vaccine ◽  
Cellular Immune Response ◽  
Lymphocyte Proliferation ◽  
Vaccine Development ◽  
Flow Cytometric Analysis ◽  
Potential Candidate ◽  
Spleen Lymphocyte ◽  
Cellular Immune ◽  
And Control

We previously reported that the chimeric DNA vaccine pcDNA-3-1E-linker-mChIL-15, fused through linkingEimeria acervulina3-1E encoding gene and mature chicken IL-15 (mChIL-15) gene with four flexible amino acid SPGS, could significantly offer protection against homologous challenge. In the present study, the induction of cellular immune response induced by the chimeric DNA vaccine pcDNA-3-1E-linker-mChIL-15 was investigated. Spleen lymphocyte subpopulations were characterized by flow cytometric analysis. The spleen lymphocyte proliferation assays were measured by 3-[4,5-dimethylthiazol-2-y1]-2,5-diphenyltetrazolium bromide (MTT) method. The mRNA profiles of ChIL-2 and ChIFN-γ in spleen were characterized by means of real-time PCR. Chickens immunized with pcDNA-3-1E-linker-mChIL-15 exhibited significant upregulated level of ChIL-2 and ChIFN-γ transcripts in spleen following two immunizations compared with chickens in other groups (P<0.01). In comparison with pcDNA3.1-immunized and control groups, lymphocyte proliferation, percentage of CD8α+cell, and levels of ChIL-2 and ChIFN-γ transcripts in the group immunized with pcDNA-3-1E-linker-mChIL-15 were significantly increased on day 6 following challenge (P<0.05,P<0.01, andP<0.01, resp.). Our data suggested that the fusion antigen 3-1E-linker-mChIL-15 could be a potential candidate forE. acervulinavaccine development.

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