scholarly journals Antibiotic resistant Vibrio harveyi isolated from shrimp pond and anti-Vibrio activity of Combretum quadrangulare Kurz. and Mimosa pudica extracts

2021 ◽  
Author(s):  
Tinrat, S. ◽  
Singhapol, C.
Keyword(s):  
Vibrio Harveyi ◽  
Mimosa Pudica ◽  
Antibiotic Resistant ◽  
Shrimp Pond

scholarly journals Biological and Genomic Characterization of a Novel Jumbo Bacteriophage, vB_VhaM_pir03 with Broad Host Lytic Activity against Vibrio harveyi

Pathogens ◽  
2020 ◽  
Vol 9 (12) ◽  
pp. 1051
Author(s):  
Gerald N. Misol ◽  
Constantina Kokkari ◽  
Pantelis Katharios
Keyword(s):  
Phage Therapy ◽  
Vibrio Harveyi ◽  
Burst Size ◽  
Disease Outbreaks ◽  
Artemia Salina ◽  
Open Reading Frames ◽  
Lytic Activity ◽  
Economic Losses ◽  
Phylogenomic Analysis ◽  
Antibiotic Resistant

Vibrio harveyi is a Gram-negative marine bacterium that causes major disease outbreaks and economic losses in aquaculture. Phage therapy has been considered as a potential alternative to antibiotics however, candidate bacteriophages require comprehensive characterization for a safe and practical phage therapy. In this work, a lytic novel jumbo bacteriophage, vB_VhaM_pir03 belonging to the Myoviridae family was isolated and characterized against V. harveyi type strain DSM19623. It had broad host lytic activity against 31 antibiotic-resistant strains of V. harveyi, V. alginolyticus, V. campbellii and V. owensii. Adsorption time of vB_VhaM_pir03 was determined at 6 min while the latent-phase was at 40 min and burst-size at 75 pfu/mL. vB_VhaM_pir03 was able to lyse several host strains at multiplicity-of-infections (MOI) 0.1 to 10. The genome of vB_VhaM_pir03 consists of 286,284 base pairs with 334 predicted open reading frames (ORFs). No virulence, antibiotic resistance, integrase encoding genes and transducing potential were detected. Phylogenetic and phylogenomic analysis showed that vB_VhaM_pir03 is a novel bacteriophage displaying the highest similarity to another jumbo phage, vB_BONAISHI infecting Vibrio coralliilyticus. Experimental phage therapy trial using brine shrimp, Artemia salina infected with V. harveyi demonstrated that vB_VhaM_pir03 was able to significantly reduce mortality 24 h post infection when administered at MOI 0.1 which suggests that it can be an excellent candidate for phage therapy.

scholarly journals Skreening Potensi Antibakteri pada Beberapa Spesies Rumput Laut terhadap Bakteri Patogen pada Udang Windu

2012 ◽  
Vol 9 (2) ◽  
pp. 62 ◽  
Author(s):  
Munifatul - Izzati
Keyword(s):  
Antibacterial Activity ◽  
Pathogenic Bacteria ◽  
Active Component ◽  
Vibrio Harveyi ◽  
Shrimp Pond ◽  
Tiger Shrimp

Antibacterial activity of several local seaweeds has been screened. Most of these seaweeds species showantibacterial properties against pathogenic bacteria from species of Vibrio and Pseudomonas. The activity ofseaweeds extracts were determined by type of solvent used in extraction. Respond of each pathogenic bacteria werealso different to each type of extract. Most seaweeds show stronger activity against Pseudomonas rather than Vibrio.Caulerpa and Padina were more active against Pseudomonas compared to Sargassum and Gelidium. Compared toother seaweeds, Sargassum and Gelidium were more active against Vibrio. It is predicted that Sargassum is the themost suitable to be used in polyculture with tiger shrimp (Peneaus monodon), as this is the most active against themost dangerous pathogenic bacteria, Vibrio harveyi. Beside that, the active component of Sargassum is solved inwater, therefore will be easier to diffuse into shrimp pond.

Mass mortality of Penaeus monodon larvae due to antibiotic-resistant Vibrio harveyi infection

Aquaculture ◽  
1994 ◽  
Vol 128 (3-4) ◽  
pp. 203-209 ◽  
Author(s):  
I. Karunasagar ◽  
R. Pai ◽  
G.R. Malathi ◽  
Indrani Karunasagar
Keyword(s):  
Vibrio Harveyi ◽  
Penaeus Monodon ◽  
Mass Mortality ◽  
Antibiotic Resistant

scholarly journals Transferable chloramphenicol resistance determinant in luminous Vibrio harveyi from penaeid shrimp Penaeus monodon larvae

2016 ◽  
Vol 4 (3) ◽  
pp. 428
Author(s):  
Thangapalam Jawahar Abraham
Keyword(s):  
Escherichia Coli ◽  
Vibrio Harveyi ◽  
Penaeus Monodon ◽  
Penaeid Shrimp ◽  
Resistance Determinant ◽  
Antibiotic Resistant ◽  
Chloramphenicol Resistance ◽  
Resistance Determinants

Antibiotic-resistant luminous Vibrio harveyi strains isolated from Penaeus monodon larvae were screened for the possession of transferable resistance determinants. All the strains were resistant to chloramphenicol and the determinant coding for chloramphenicol resistance was transferred to Escherichia coli at frequencies of 9.50x10-4 to 4.20x10-4. The results probably suggest the excessive use of chloramphenicol in shrimp hatcheries to combat luminous vibriosis.

THE BACTERIAL MULTIPLE ANTIBIOTIC RESISTANT (MAR) PHENOTYPE LEADS TO INCREASED TOLERANCE TO TEA TREE OIL

Pathology ◽  
2001 ◽  
Vol 33 (2) ◽  
pp. 211-215 ◽  
Author(s):  
John E. Gustafson ◽  
Sean D. Cox ◽  
Yoon C. Liew ◽  
S. Grant Wyllie ◽  
John R. Warmington
Keyword(s):  
Tea Tree Oil ◽  
Antibiotic Resistant ◽  
Tea Tree

Effects of abiotic and biotic factors on Vibrio harveyi ATCC 14126T survival dynamics in seawater microcosms

2019 ◽  
Vol 83 (2) ◽  
pp. 109-118 ◽  
Author(s):  
M Orruño ◽  
C Parada ◽  
E Ogayar ◽  
VR Kaberdin ◽  
I Arana
Keyword(s):  
Vibrio Harveyi ◽  
Biotic Factors ◽  
Abiotic And Biotic Factors

Vibrio harveyi virulence gene expression in vitro and in vivo during infection in black tiger shrimp Penaeus monodon

2020 ◽  
Vol 139 ◽  
pp. 153-160
Author(s):  
S Peeralil ◽  
TC Joseph ◽  
V Murugadas ◽  
PG Akhilnath ◽  
VN Sreejith ◽  
...  
Keyword(s):  
Gene Expression ◽  
Virulence Genes ◽  
Vibrio Harveyi ◽  
Penaeus Monodon ◽  
Challenge Test ◽  
Virulence Gene ◽  
Economic Losses ◽  
Virulence Gene Expression

Luminescent Vibrio harveyi is common in sea and estuarine waters. It produces several virulence factors and negatively affects larval penaeid shrimp in hatcheries, resulting in severe economic losses to shrimp aquaculture. Although V. harveyi is an important pathogen of shrimp, its pathogenicity mechanisms have yet to be completely elucidated. In the present study, isolates of V. harveyi were isolated and characterized from diseased Penaeus monodon postlarvae from hatcheries in Kerala, India, from September to December 2016. All 23 tested isolates were positive for lipase, phospholipase, caseinase, gelatinase and chitinase activity, and 3 of the isolates (MFB32, MFB71 and MFB68) showed potential for significant biofilm formation. Based on the presence of virulence genes, the isolates of V. harveyi were grouped into 6 genotypes, predominated by vhpA+ flaB+ ser+ vhh1- luxR+ vopD- vcrD+ vscN-. One isolate from each genotype was randomly selected for in vivo virulence experiments, and the LD50 ranged from 1.7 ± 0.5 × 103 to 4.1 ± 0.1 × 105 CFU ml-1. The expression of genes during the infection in postlarvae was high in 2 of the isolates (MFB12 and MFB32), consistent with the result of the challenge test. However, in MFB19, even though all genes tested were present, their expression level was very low and likely contributed to its lack of virulence. Because of the significant variation in gene expression, the presence of virulence genes alone cannot be used as a marker for pathogenicity of V. harveyi.

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