scholarly journals Enhancement of protease production by the optimization of Bacillus subtilis culture medium

2013 ◽  
Author(s):  
Cheong, J.Y. ◽  
Mustafa, M. ◽  
Abd. Aziz, N.A. ◽  
Go, R. ◽  
Ahmad Adli, A.
Keyword(s):  
Bacillus Subtilis ◽  
Culture Medium ◽  
Protease Production

scholarly journals The influence of Bacillus subtilis on tin-coated copper in an aqueous environment

RSC Advances ◽  
2018 ◽  
Vol 8 (9) ◽  
pp. 4671-4679
Author(s):  
Ruilin Xiong ◽  
Kui Xiao ◽  
Pan Yi ◽  
Yuting Hu ◽  
Chaofang Dong ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Culture Medium ◽  
Corrosion Behaviour ◽  
Aqueous Environment

The effect of Bacillus subtilis (BS) on the corrosion behaviour of tin-coated copper was investigated by exposing the sample to a culture medium inoculated with BS.

Protease production by Bacillus subtilis in oxygen-controlled, glucose fed-batch fermentations

1988 ◽  
Vol 28 (4-5) ◽  
pp. 404-408 ◽  
Author(s):  
Manoj M. Kole ◽  
Indira Draper ◽  
Donald F. Gerson
Keyword(s):  
Bacillus Subtilis ◽  
Protease Production ◽  
Fed Batch

Production and characterisation of thermostable alkaline protease from Bacillus subtilis isolated from LA hot spring, Terengganu

2021 ◽  
Vol 16 (7) ◽  
pp. 84-91
Author(s):  
Maslinda Alias ◽  
Hakim Che Harun Mohammad ◽  
Ashraf Razali Nurul ◽  
Jasnizat Saidin ◽  
Nazaitulshila Rasit ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Alkaline Protease ◽  
Protease Activity ◽  
Hot Spring ◽  
Skim Milk ◽  
Industrial Applications ◽  
Protease Production ◽  
Significant Activity ◽  
Original Activity ◽  
Optimum Ph

This research aims to produce thermostable alkaline protease from Bacillus subtilis isolated from La Hot Spring, Terengganu, Malaysia. The study was also conducted to determine the optimum conditions for protease production and stability by considering several parameters including pH, temperature and salt concentration. All seven bacteria were screened on skim milk agar overnight at 37 °C. Three strains with the highest proteolytic activity were identified in protease specific medium. The thermostable alkaline protease had an optimum temperature of 60 °C which achieved 85.73, 82.90 and 83.05 U/mL of protease activity for the three strains respectively. Furthermore, the strains exhibited significant activity of more than 90% from their original activity. Meanwhile, the optimum pH for protease production was pH 9 with the protease activity of 76.76, 79.71 and 88.39 U/mL for TB4, TB6 and TB9 strains, respectively. Proteases were found stable at pH 9 where the loss did not exceed 30% of its original activity. Collectively, all of the data emphasised that proteases from B. subtilis were alkaline thermostable proteases in accordance with a recent report. The finding highlights the viability of the proteases for biotechnological and industrial applications.

scholarly journals Optimization of Medium Ingredients for Keratinolytic Protease Production by Bacillus licheniformis MZK-03 using Statistical Experimental Designs

1970 ◽  
Vol 24 (1) ◽  
pp. 52-56 ◽  
Author(s):  
Mohammad Moniruzzaman ◽  
Alamgir Rahman ◽  
M Mozammel Hoq
Keyword(s):  
Trace Elements ◽  
Bacillus Licheniformis ◽  
Shake Flask ◽  
Culture Medium ◽  
Protease Production ◽  
Shake Flask Culture ◽  
Flask Culture ◽  
Statistical Approaches ◽  
Central Composite ◽  
Keratinolytic Protease

A culture medium was optimized for the production of keratinolytic protease by a newly isolated strain of Bacillus licheniformis MZK-03 in shake-flask culture. Based on the results of preliminary experiments, feather mill, molasses and trace elements were found to be major variables in keratinolytic protease production. The concentrations of these ingredients were optimized by using two statistical approaches, namely Box-Wilson method and central composite design. The optimized culture medium, finally determined by using the statistical approaches, composed of 0.95% feather mill, 0.12% molasses and 1.44% trace elements. The keratinolytic protease production was increased by approximately 2-fold when the strain was grown in the optimized medium (95.2 U/ml) compared to the un-optimized medium (56.05 U/ml). Keywords: Keratinolytic protease, Optimization, Bacillus licheniformis MZK-03, Statistical designsDOI: http://dx.doi.org/10.3329/bjm.v24i1.1238 Bangladesh J Microbiol, Volume 24, Number 1, June 2007, pp 52-56

scholarly journals Response surface optimization of medium composition for protease production by Bacillus subtilis using cassava waste

2011 ◽  
Vol 17 (2) ◽  
pp. 215-222 ◽  
Author(s):  
P. Rathakrishnan ◽  
P. Nagarajan ◽  
Rajesh Kannan
Keyword(s):  
Bacillus Subtilis ◽  
Response Surface ◽  
Waste Product ◽  
Maximum Growth ◽  
Positive Influence ◽  
Maximum Growth Rate ◽  
Medium Composition ◽  
Protease Production ◽  
Response Surface Optimization ◽  
Cassava Waste

Optimization of the growth condition for maximum growth rate and protease production was carried out using Bacillus subtilis. The optimization of protease production using agro industrial waste product such as cassava waste as substrate was performed with statistical methodology based on experimental designs. The screening of twelve nutrients for their influence on protease production was achieved using a Plackett-Burman design. MgSO4.7H2O, casein and glucose were selected based on their positive influence on protease production. The selected components were optimized using Response Surface Methodology (RSM). The optimum conditions are (% w/w): MgsO4.7H2O- 0.14, casein- 1.4 and glucose- 2.64. These conditions were validated experimentally which revealed an enhanced protease yield of 202.048 U/gds.

Alkaline Protease Production Using Response Surface Methodology, Characterization and Industrial Exploitation of Alkaline Protease of Bacillus subtilis sp.

2017 ◽  
Vol 147 (5) ◽  
pp. 1204-1213 ◽  
Author(s):  
Fouzia Hussain ◽  
Shagufta Kamal ◽  
Saima Rehman ◽  
Muhammad Azeem ◽  
Ismat Bibi ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Bacillus Subtilis ◽  
Response Surface ◽  
Alkaline Protease ◽  
Protease Production

scholarly journals Improvement of α-Amylase Production by Modulation of Ribosomal Component Protein S12 in Bacillus subtilis 168

2006 ◽  
Vol 72 (1) ◽  
pp. 71-77 ◽  
Author(s):  
Kazuhiko Kurosawa ◽  
Takeshi Hosaka ◽  
Norimasa Tamehiro ◽  
Takashi Inaoka ◽  
Kozo Ochi
Keyword(s):  
Bacillus Subtilis ◽  
Antibiotic Production ◽  
Point Mutations ◽  
Protease Production ◽  
Gene Encoding ◽  
Translational Activity ◽  
Amylase Production ◽  
Ribosomal Protein S12

ABSTRACT The capacity of ribosomal modification to improve antibiotic production by Streptomyces spp. has already been demonstrated. Here we show that introduction of mutations that produce streptomycin resistance (str) also enhances α-amylase (and protease) production by a strain of Bacillus subtilis as estimated by measuring the enzyme activity. The str mutations are point mutations within rpsL, the gene encoding the ribosomal protein S12. In vivo as well as in vitro poly(U)-directed cell-free translation systems showed that among the various rpsL mutations K56R (which corresponds to position 42 in E. coli) was particularly effective at enhancing α-amylase production. Cells harboring the K56R mutant ribosome exhibited enhanced translational activity during the stationary phase of cell growth. In addition, the K56R mutant ribosome exhibited increased 70S complex stability in the presence of low Mg2+ concentrations. We therefore conclude that the observed increase in protein synthesis activity by the K56R mutant ribosome reflects increased stability of the 70S complex and is responsible for the increase in α-amylase production seen in the affected strain.

scholarly journals Production of Enzymes from Agroindustrial Wastes by Biosurfactant-Producing Strains of Bacillus subtilis

2013 ◽  
Vol 2013 ◽  
pp. 1-9 ◽  
Author(s):  
Francisco Fábio Cavalcante Barros ◽  
Ana Paula Resende Simiqueli ◽  
Cristiano José de Andrade ◽  
Gláucia Maria Pastore
Keyword(s):  
Bacillus Subtilis ◽  
Culture Medium ◽  
Synthetic Medium ◽  
Lipase Production ◽  
Industrial Processes ◽  
Technical Literature ◽  
Wide Range ◽  
Cassava Wastewater ◽  
Agroindustrial Wastes ◽  
Better Than

Bacteria in the genus Bacillus are the source of several enzymes of current industrial interest. Hydrolases, such as amylases, proteases, and lipases, are the main enzymes consumed worldwide and have applications in a wide range of products and industrial processes. Fermentation processes by Bacillus subtilis using cassava wastewater as a substrate are reported in the technical literature; however, the same combination of microorganisms and this culture medium is limited or nonexistent. In this paper, the amylase, protease, and lipase production of ten Bacillus subtilis strains previously identified as biosurfactant producers in cassava wastewater was evaluated. The LB1a and LB5a strains were selected for analysis using a synthetic medium and cassava wastewater and were identified as good enzyme producers, especially of amylases and proteases. In addition, the enzymatic activity results indicate that cassava wastewater was better than the synthetic medium for the induction of these enzymes.

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