Response surface optimization of medium composition for protease production by Bacillus subtilis using cassava waste

Optimization of the growth condition for maximum growth rate and protease production was carried out using Bacillus subtilis. The optimization of protease production using agro industrial waste product such as cassava waste as substrate was performed with statistical methodology based on experimental designs. The screening of twelve nutrients for their influence on protease production was achieved using a Plackett-Burman design. MgSO4.7H2O, casein and glucose were selected based on their positive influence on protease production. The selected components were optimized using Response Surface Methodology (RSM). The optimum conditions are (% w/w): MgsO4.7H2O- 0.14, casein- 1.4 and glucose- 2.64. These conditions were validated experimentally which revealed an enhanced protease yield of 202.048 U/gds.

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Response surface optimization of medium composition for xylitol production by Debaryomyces hansenii var hansenii using corncob hemicellulose hydrolysate

Chemical Industry and Chemical Engineering Quarterly ◽

10.2298/ciceq120315072r ◽

2013 ◽

Vol 19 (3) ◽

pp. 377-384 ◽

Cited By ~ 2

Author(s):

S. Ramesh ◽

R. Muthuvelayudham ◽

Rajesh Kannan ◽

T. Viruthagiri

Keyword(s):

Response Surface ◽

Yeast Extract ◽

Debaryomyces Hansenii ◽

Positive Influence ◽

Medium Composition ◽

Xylitol Production ◽

Hemicellulose Hydrolysate ◽

Response Surface Optimization ◽

Burman Design ◽

Plackett Burman Design

Optimization of the culture medium for xylitol production using Debrayomyces hansenii var hansenii was carried out. The optimization of xylitol production using corncob hemicelluloses hydrolysate as substrate was performed with statistical methodology based on experimental designs. The screening of nine nutrients for their influence of xylitol production to achieved using a Plackett-Burman design. MgSO4.7H2O, KH2PO4, (NH4)2SO4, yeast extract were selected for based on their positive influence on xylitol production. The selected components were optimized using Response Surface Methodology (RSM). The optimum conditions are: MgSO4.7H2O - 1.02 g/l, (NH4)2SO4 - 3.94 g/l, KH2PO4- 2.74 g/l and yeast extract - 3.45 g/l. These conditions are validated experimentally which revealed an enhanced xylitol yield of 0.76 g/g.

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Response surface optimization of medium composition for alkaline protease production by Bacillus clausii

Biochemical Engineering Journal ◽

10.1016/j.bej.2007.08.016 ◽

2008 ◽

Vol 39 (1) ◽

pp. 37-42 ◽

Cited By ~ 53

Author(s):

Seyedeh Faranak Ghaemi Oskouie ◽

Fatemeh Tabandeh ◽

Bagher Yakhchali ◽

Fereshteh Eftekhar

Keyword(s):

Response Surface ◽

Alkaline Protease ◽

Medium Composition ◽

Protease Production ◽

Bacillus Clausii ◽

Response Surface Optimization ◽

Surface Optimization

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Optimizing of Microalgae Scenedesmus sp. Biomass Production in Wet Market Wastewater Using Response Surface Methodology

Sustainability ◽

10.3390/su13042216 ◽

2021 ◽

Vol 13 (4) ◽

pp. 2216

Author(s):

Najeeha Mohd Apandi ◽

Mimi Suliza Muhamad ◽

Radin Maya Saphira Radin Mohamed ◽

Norshuhaila Mohamed Sunar ◽

Adel Al-Gheethi ◽

...

Keyword(s):

Response Surface Methodology ◽

Response Surface ◽

Biomass Yield ◽

Biomass Productivity ◽

Maximum Growth ◽

Maximum Growth Rate ◽

Aeration Rate ◽

Composition Analysis ◽

Wet Market ◽

Central Composite

The present study aimed to optimize the production of Scenedesmus sp. biomass during the phycoremediation process. The biomass productivity was optimized using face centred central composite design (FCCCD) in response surface methodology (RSM) as a function of two independent variables that included wet market wastewater concentrations (A) with a range of 10% to 75% and aeration rate (B) with a range of 0.02 to 4.0 L/min. The results revealed that the highest biomass productivity (73 mg/L/d) and maximum growth rate (1.19 day−1) was achieved with the 64.26% of (A) and 3.08 L/min of (B). The GC-MS composition analysis of the biomass yield extract revealed that the major compounds are hexadecane (25%), glaucine (16.2%), and phytol (8.33%). The presence of these compounds suggests that WMW has the potential to be used as a production medium for Scenedesmus sp. Biomass, which has several applications in the pharmaceutical and chemical industry.

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