scholarly journals Antibiotic resistance and molecular characterization of enteroaggregative Escherichia coli isolated from patients with diarrhea in the Eastern Province of Saudi Arabia

Heliyon ◽  
2020 ◽  
Vol 6 (4) ◽  
pp. e03721 ◽  
Author(s):  
Nasreldin Elhadi ◽  
Reem Aljindan ◽  
Khaldoon Alsamman ◽  
Amer Alomar ◽  
Mohammed Aljeldah
Keyword(s):  
Escherichia Coli ◽  
Saudi Arabia ◽  
Antibiotic Resistance ◽  
Molecular Characterization ◽  
Eastern Province

scholarly journals Antibiotic resistance and molecular characterization of bacteremia Escherichia coli isolates from newborns in the United States

PLoS ONE ◽  
2019 ◽  
Vol 14 (7) ◽  
pp. e0219352 ◽  
Author(s):  
Bryan K. Cole ◽  
Marko Ilikj ◽  
Cindy B. McCloskey ◽  
Susana Chavez-Bueno
Keyword(s):  
United States ◽  
Escherichia Coli ◽  
Antibiotic Resistance ◽  
Molecular Characterization ◽  
The United States

scholarly journals Antibiotic resistance of Escherichia coli isolated from poultry workers, patients and chicken in the eastern province of Saudi Arabia

1999 ◽  
Vol 4 (4) ◽  
pp. 278-283 ◽  
Author(s):  
Mastour S. Al-Ghamdi ◽  
Fikry El-Morsy ◽  
Zaki H. Al-Mustafa ◽  
Mustafa Al-Ramadhan ◽  
Mohammad Hanif
Keyword(s):  
Escherichia Coli ◽  
Saudi Arabia ◽  
Antibiotic Resistance ◽  
Eastern Province ◽  
Poultry Workers

Prevalence, antibiotic resistance patterns and molecular characterization of Escherichia coli from Austrian sandpits

2014 ◽  
Vol 194 ◽  
pp. 24-30 ◽  
Author(s):  
Alexandra Badura ◽  
Josefa Luxner ◽  
Gebhard Feierl ◽  
Franz F. Reinthaler ◽  
Gernot Zarfel ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antibiotic Resistance ◽  
Molecular Characterization ◽  
Resistance Patterns ◽  
Antibiotic Resistance Patterns

scholarly journals Serological and Molecular Characterization of Mycobacterium avium Subsp. paratuberculosis (MAP) from Sheep, Goats, Cattle and Camels in the Eastern Province, Saudi Arabia

Animals ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 323
Author(s):  
Ibrahim Elsohaby ◽  
Mahmoud Fayez ◽  
Mohamed Alkafafy ◽  
Mohamed Refaat ◽  
Theeb Al-Marri ◽  
...  
Keyword(s):  
Saudi Arabia ◽  
Molecular Characterization ◽  
Mycobacterium Avium ◽  
Mycobacterium Avium Subsp ◽  
Type I ◽  
Serum Samples ◽  
Eastern Province ◽  
Fecal Samples ◽  
Mycobacterium Avium Subsp Paratuberculosis

The objectives of the present study were to characterize Mycobacterium avium subsp. paratuberculosis (MAP) infection using serological and molecular tools and investigate the distribution and molecular characterization of MAP strains (cattle (C) and sheep (S) types) in sheep, goat, cattle, and camel herds in Eastern Province, Saudi Arabia. Serum and fecal samples were collected from all animals aged >2 years old in 31 herds (sheep = 8, goats = 6, cattle = 8 and camels = 9) from January to December 2019. Serum samples were tested by ELISA for the detection of MAP antibodies. Fecal samples were tested by PCR for the detection of MAP IS900 gene and the identification of MAP strains. MAP antibodies were detected in 19 (61.3%) herds. At the animal level, antibodies against MAP were detected in 43 (19.5%) sheep, 21 (17.1%) goats, 13 (19.7%) cattle and 22 (9.1%) camels. The IS900 gene of MAP was detected in 23 (74.2%) herds and was directly amplified from fecal samples of 59 (26.8%) sheep, 34 (27.6%) goats, 20 (30.3%) cattle and 36 (15.0%) camels. The S-type was the most prevalent MAP type identified in 15 herds, and all were identified as type-I, while the C-type was identified in only 8 herds. The IS900 sequences revealed genetic differences among the MAP isolates recovered from sheep, goats, cattle and camels. Results from the present study show that MAP was prevalent and confirm the distribution of different MAP strains in sheep, goat, cattle and camel herds in Eastern Province, Saudi Arabia.

Molecular Characterization of Antibiotic Resistance Gene Pattern of Staphylococcus aureus and Escherichia coli in Mastitis Affected Dairy Cows

2020 ◽  
Author(s):  
T. Ramasamy ◽  
S. Keerthana ◽  
M.R. Srinivasan ◽  
D. Chandrasekar ◽  
K. Porteen ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibiotic Resistance ◽  
Antimicrobial Resistance ◽  
Molecular Characterization ◽  
Antibiotic Sensitivity ◽  
Growth Media ◽  
E Coli ◽  
Sensitivity Pattern

Background: Antimicrobial resistance is one of the latest challenges facing the scientific community. Raising the drug resistance is caused mainly by indiscriminate usage of antibiotics in human and animal subjects and the spread of antibiotic resistance between the two has an emerging global threat. Hence, current study aimed to study the antimicrobial resistance pattern and molecular detection of antibiotic resistance genes in Staphylococcus aureus and Escherichia coli isolated from mastitis affected cows. Methods: Milk samples from mastitis affected cows were subjected to antibiotic sensitivity test and screened for presence of Staphylococcus aureus and Escherichia coli using differential growth media. Molecular characterization of Staphylococcus aureus and Escherichia coli was done with the help of PCR by amplification of ‘nuc’ and ‘uspA’ gene respectively. MICs of Penicillin and Tetracycline were determined using microdilution method. Result: Antibiotic sensitivity pattern for Penicillin G, Ampicillin, Amoxycillin, Cefotaxime, Ceftriaxone, Azithromycin, Ciprofloxacin, Gentamicin, Oxytetracycline, Tetracycline and Vancomycin were 74.19%, 100%, 93.50%, 61.29%, 29%, 35.48%, 9.70%, 9.70%, 70.96% and 70.96% respectively. More than 87.90% of the S. aureus and 50% of the E. coli isolated were resistant to â-lactam antibiotics while 75% of the E.coli and 65.70% of the S. aureus isolated were resistant to Tetracycline antibiotics. The MICs of Penicillin for S.aureus and E.coli are 26.88 µg/ml and 13.54 µg/ml respectively and the MICs of Tetracycline for S. aureus and E. coli are 243.75 µg/ml and 960.93 µg/ml respectively which is 8-9 folds higher than the standard MICs. From the present study, it can be inferred that bovine mastitis cases are highly resistant to antimicrobial drugs. Results further indicate that Staphylococcus aureus and Escherichia coli are both resistant to Penicillin and Tetracycline with very high MIC.

scholarly journals Molecular Characterization of a 21.4 Kilobase Antibiotic Resistance Plasmid from an α-Hemolytic Escherichia coli O108:H- Human Clinical Isolate

PLoS ONE ◽  
2012 ◽  
Vol 7 (4) ◽  
pp. e34718
Author(s):  
Fay E. Dawes ◽  
Dieter M. Bulach ◽  
Alexander Kuzevski ◽  
Karl A. Bettelheim ◽  
Carola Venturini ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antibiotic Resistance ◽  
Molecular Characterization ◽  
Clinical Isolate ◽  
Resistance Plasmid

scholarly journals Molecular characterization of Escherichia coli O157:H7 recovered from meat and meat products relevant to human health in Riyadh, Saudi Arabia

2015 ◽  
Vol 22 (6) ◽  
pp. 725-729 ◽  
Author(s):  
Ashgan M. Hessain ◽  
Abdullah A. Al-Arfaj ◽  
Adel M. Zakri ◽  
Jakeen K. El-Jakee ◽  
Onizan G. Al-Zogibi ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Saudi Arabia ◽  
Human Health ◽  
Molecular Characterization ◽  
Meat Products ◽  
Escherichia Coli O157
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