scholarly journals GhYGL1, a pentatricopeptide repeat protein, is required for chloroplast development in cotton

2019 ◽  
Author(s):  
Peng He ◽  
Shuyin Wu ◽  
Yanli Jiang ◽  
Lihua Zhang ◽  
Meiju Tang ◽  
...  
Keyword(s):  
Amino Acid ◽  
Gene Families ◽  
Pentatricopeptide Repeat ◽  
Amino Acid Sequence Homology ◽  
Plastid Development ◽  
Amino Acid Repeats ◽  
Leaf Variegation ◽  
Leaf Phenotype ◽  
High Amino Acid ◽  
Ppr Genes

Abstract Background The pentatricopeptide repeat (PPR) gene family, which contains multiple 35-amino acid repeats, constitutes one of the largest gene families in plants. Pentatricopeptide repeat genes function in organelles to target specific transcripts and are involved in plant development and growth. However, the function of PPR genes in cotton is still unknown. Results In this study, we characterized a PPR gene (GhYGL1), producing a yellow green leaf phenotype, that is required for cotton plastid development. The GhYGL1 gene has a DYW domain in C-terminal and is highly express in leaves, localized to the chloroplast fractions. GhYGL1 share high amino acid-sequence homology with AtECB2. In ecb2-mutated Arabidopsis, overexpression of GhYGL1 rescued the seedling lethal phenotype and restored the editing of accD and ndhF transcripts. Silencing of GhYGL1 led to the reduction of chlorophyll and phenotypically yellow-green leaves in cotton. Compared with wild type, GhYGL1-silenced cotton showed significant deformations of thylakoid structures. Furthermore, the expression levels of plastid-encoded polymerase- (PEP) and nuclear-encoded polymerase- (NEP) dependent genes were significantly decreased in GhYGL1-silenced cotton. Conclusions Our data indicate that GhYGL1 not only controls the editing of accD and ndhF genes, but also controls the expression of NEP- and PEP-dependent genes to regulate the development of thylakoids, and therefore regulates leaf variegation in cotton.

scholarly journals GhYGL1d, a pentatricopeptide repeat protein, is required for chloroplast development in cotton

2019 ◽  
Author(s):  
Peng He ◽  
Shuyin Wu ◽  
Yanli Jiang ◽  
Lihua Zhang ◽  
Meiju Tang ◽  
...  
Keyword(s):  
Amino Acid ◽  
Gene Families ◽  
Wild Type ◽  
Pentatricopeptide Repeat ◽  
Amino Acid Sequence Homology ◽  
Plastid Development ◽  
Amino Acid Repeats ◽  
Leaf Variegation ◽  
High Amino Acid ◽  
Ppr Proteins

Abstract Background: The pentatricopeptide repeat (PPR) gene family, which contains multiple 35-amino acid repeats, constitutes one of the largest gene families in plants. PPR proteins function in organelles to target specific transcripts and are involved in plant development and growth. However, the function of PPR proteins in cotton is still unknown. Results: In this study, we characterized a PPR gene YELLOW-GREEN LEAF (GhYGL1d) that is required for cotton plastid development. The GhYGL1d gene has a DYW domain in C-terminal and is highly express in leaves, localized to the chloroplast fractions. GhYGL1d share high amino acid-sequence homology with AtECB2. In atecb2 mutant, overexpression of GhYGL1d rescued the seedling lethal phenotype and restored the editing of accD and ndhF transcripts. Silencing of GhYGL1d led to the reduction of chlorophyll and phenotypically yellow-green leaves in cotton. Compared with wild type, GhYGL1d-silenced cotton showed significant deformations of thylakoid structures. Furthermore, the transcription levels of plastid-encoded polymerase (PEP) and nuclear-encoded polymerase (NEP) dependent genes were decreased in GhYGL1d-silenced cotton. Conclusions: Our data indicate that GhYGL1d not only contributes to the editing of accD and ndhF genes, but also affects the expression of NEP- and PEP-dependent genes to regulate the development of thylakoids, and therefore regulates leaf variegation in cotton.

scholarly journals GhYGL1d, a pentatricopeptide repeat protein, is required for chloroplast development in cotton

2019 ◽  
Author(s):  
Peng He ◽  
Shuyin Wu ◽  
Yanli Jiang ◽  
Lihua Zhang ◽  
Meiju Tang ◽  
...  
Keyword(s):  
Amino Acid ◽  
Gene Families ◽  
Wild Type ◽  
Pentatricopeptide Repeat ◽  
Amino Acid Sequence Homology ◽  
Plastid Development ◽  
Amino Acid Repeats ◽  
Leaf Variegation ◽  
High Amino Acid ◽  
Ppr Proteins

Abstract Background: The pentatricopeptide repeat (PPR) gene family, which contains multiple 35-amino acid repeats, constitutes one of the largest gene families in plants. PPR proteins function in organelles to target specific transcripts and are involved in plant development and growth. However, the function of PPR proteins in cotton is still unknown. Results: In this study, we characterized a PPR gene YELLOW-GREEN LEAF (GhYGL1d) that is required for cotton plastid development. The GhYGL1d gene has a DYW domain in C-terminal and is highly express in leaves, localized to the chloroplast fractions. GhYGL1d share high amino acid-sequence homology with AtECB2. In atecb2 mutant, overexpression of GhYGL1d rescued the seedling lethal phenotype and restored the editing of accD and ndhF transcripts. Silencing of GhYGL1d led to the reduction of chlorophyll and phenotypically yellow-green leaves in cotton. Compared with wild type, GhYGL1d-silenced cotton showed significant deformations of thylakoid structures. Furthermore, the transcription levels of plastid-encoded polymerase (PEP) and nuclear-encoded polymerase (NEP) dependent genes were decreased in GhYGL1d-silenced cotton. Conclusions: Our data indicate that GhYGL1d not only contributes to the editing of accD and ndhF genes, but also affects the expression of NEP- and PEP-dependent genes to regulate the development of thylakoids, and therefore regulates leaf variegation in cotton.

scholarly journals GhYGL1d, a pentatricopeptide repeat protein, is required for chloroplast development in cotton

2019 ◽  
Author(s):  
Peng He ◽  
Shuyin Wu ◽  
Yanli Jiang ◽  
Lihua Zhang ◽  
Meiju Tang ◽  
...  
Keyword(s):  
Amino Acid ◽  
Gene Families ◽  
Wild Type ◽  
Pentatricopeptide Repeat ◽  
Amino Acid Sequence Homology ◽  
Plastid Development ◽  
Amino Acid Repeats ◽  
Leaf Variegation ◽  
High Amino Acid ◽  
Ppr Proteins

Abstract Background: The pentatricopeptide repeat (PPR) gene family, which contains multiple 35-amino acid repeats, constitutes one of the largest gene families in plants. PPR proteins function in organelles to target specific transcripts and are involved in plant development and growth. However, the function of PPR proteins in cotton is still unknown. Results: In this study, we characterized a PPR gene YELLOW-GREEN LEAF (GhYGL1d) that is required for cotton plastid development. The GhYGL1d gene has a DYW domain in C-terminal and is highly express in leaves, localized to the chloroplast fractions. GhYGL1d share high amino acid-sequence homology with AtECB2. In atecb2 mutant, overexpression of GhYGL1d rescued the seedling lethal phenotype and restored the editing of accD and ndhF transcripts. Silencing of GhYGL1d led to the reduction of chlorophyll and phenotypically yellow-green leaves in cotton. Compared with wild type, GhYGL1d-silenced cotton showed significant deformations of thylakoid structures. Furthermore, the transcription levels of plastid-encoded polymerase (PEP) and nuclear-encoded polymerase (NEP) dependent genes were decreased in GhYGL1d-silenced cotton. Conclusions: Our data indicate that GhYGL1d not only contributes to the editing of accD and ndhF genes, but also affects the expression of NEP- and PEP-dependent genes to regulate the development of thylakoids, and therefore regulates leaf variegation in cotton.

scholarly journals The Nature and Arrangement of Pentatricopeptide Domains and the Linker Sequences Between Them

2020 ◽  
Vol 14 ◽  
pp. 117793222090643 ◽  
Author(s):  
Sailen Barik
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Protein Function ◽  
Bioinformatic Analysis ◽  
Acid Number ◽  
Tetratricopeptide Repeat ◽  
Pentatricopeptide Repeat ◽  
Alpha Helices ◽  
Common Amino Acid ◽  
Amino Acid Repeats

The tricopeptide (amino acid number in the 30s) repeats constitute some of the most common amino acid repeats in proteins of diverse organisms. The most important representatives of this class are the 34-residue and 35-residue repeats, eponymously known as tetratricopeptide repeat (TPR) and pentatricopeptide repeat (PPR), respectively. The unit motif of both consists of a pair of alpha helices. As members of the large, all-helical repeat classes, TPR and PPR share structural similarities, but also play specific roles in protein function. In this study, a comprehensive bioinformatic analysis of the PPR units and the linkers that connect them was conducted. The results suggested the existence of PPR repeats of various formats, as well as smaller, PPR-unrelated repeats. Besides their length, these repeats differed in amino acid arrangements and location of key amino acids. These findings provide a broader and unified perspective of the pentatricopeptide family while raising provocative questions about the assembly and evolution of these domains.

scholarly journals Amino Acid Repeats Cause Extraordinary Coding Sequence Variation in the Social Amoeba Dictyostelium discoideum

PLoS ONE ◽  
2012 ◽  
Vol 7 (9) ◽  
pp. e46150 ◽  
Author(s):  
Clea Scala ◽  
Xiangjun Tian ◽  
Natasha J. Mehdiabadi ◽  
Margaret H. Smith ◽  
Gerda Saxer ◽  
...  
Keyword(s):  
Amino Acid ◽  
Dictyostelium Discoideum ◽  
Sequence Variation ◽  
Social Amoeba ◽  
Coding Sequence ◽  
Amino Acid Repeats ◽  
The Social

scholarly journals A PPR Protein ACM1 Is Involved in Chloroplast Gene Expression and Early Plastid Development in Arabidopsis

2021 ◽  
Vol 22 (5) ◽  
pp. 2512
Author(s):  
Xinwei Wang ◽  
Yaqi An ◽  
Ye Li ◽  
Jianwei Xiao
Keyword(s):  
Fluorescent Protein ◽  
Chloroplast Development ◽  
Nuclear Gene ◽  
Pentatricopeptide Repeat ◽  
Plastid Development ◽  
Chloroplast Gene Expression ◽  
Knock Out ◽  
Rnai Line ◽  
Ppr Protein ◽  
P Type

Chloroplasts cannot develop normally without the coordinated action of various proteins and signaling connections between the nucleus and the chloroplast genome. Many questions regarding these processes remain unanswered. Here, we report a novel P-type pentatricopeptide repeat (PPR) factor, named Albino Cotyledon Mutant1 (ACM1), which is encoded by a nuclear gene and involved in chloroplast development. Knock-down of ACM1 transgenic plants displayed albino cotyledons but normal true leaves, while knock-out of the ACM1 gene in seedlings was lethal. Fluorescent protein analysis showed that ACM1 was specifically localized within chloroplasts. PEP-dependent plastid transcript levels and splicing efficiency of several group II introns were seriously affected in cotyledons in the RNAi line. Furthermore, denaturing gel electrophoresis and Western blot experiments showed that the accumulation of chloroplast ribosomes was probably damaged. Collectively, our results indicate ACM1 is indispensable in early chloroplast development in Arabidopsis cotyledons.

Biochemical characterization of a glucoamylase from Saccharomycopsis fibuligera R64

Biologia ◽  
2011 ◽  
Vol 66 (1) ◽  
Author(s):  
Dessy Natalia ◽  
Keni Vidilaseris ◽  
Pasjan Satrimafitrah ◽  
Wangsa Ismaya ◽  
Purkan ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Amino Acid ◽  
Amino Acid Sequence Identity ◽  
Biochemical Characterization ◽  
Saccharomycopsis Fibuligera ◽  
Sequence Identity ◽  
Ic50 Value ◽  
High Amino Acid ◽  
Ph Range

AbstractGlucoamylase from the yeast Saccharomycopsis fibuligera R64 (GLL1) has successfully been purified and characterized. The molecular mass of the enzyme was 56,583 Da as determined by mass spectrometry. The purified enzyme demonstrated optimum activity in the pH range of 5.6–6.4 and at 50°C. The activity of the enzyme was inhibited by acarbose with the IC50 value of 5 μM. GLL1 shares high amino acid sequence identity with GLU1 and GLA1, which are Saccharomycopsis fibuligera glucoamylases from the strains HUT7212 and KZ, respectively. The properties of GLL1, however, resemble that of GLU1. The elucidation of the primary structure of GLL1 contributes to the explanation of this finding.

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