Telomere Length in Patients with Transfusion-Dependent Thalassemia

Abstract Background: Thalassemia is a hereditary hemolytic anemia with a severity ranging from mild, non transfusion-dependent to severe chronic anemia requiring lifelong transfusion. Transfusional iron overload is a major complication in patients with transfusion-dependent thalassemia (TDT). Telomeres are sequences of nucleotides forming the end caps of chromosomes that act as a DNA repair system. Iron overload in thalassemia can cause increased oxidative stress which leads to cellular damage and senescence. This may result in telomere length shortening. The degree of telomere length shortening may reflect the severity of thalassemia. Methods: This research aimed to study the telomere length in patients with TDT in comparison to non-thalassemic individuals and to identify the clinical and laboratory parameters that are associated with telomere length. We conducted a cross-sectional study in patients with TDT aged ³18 years. Telomere length was measured by real-time quantitative PCR. Results: Sixty-five patients with TDT were enrolled onto the study. There were 37 female patients (54.4%). The median age was 27 (18-57) years, and mean pre-transfusion hemoglobin (Hb) was 7.1 (± 1.07) g/dL. The mean telomeric terminal restriction fragment length (TRFL) of patients with TDT and the controls was 6.11 (± 0.61) kb and 6.79 (± 0.84) kb, respectively (p <0.0001). There was a significant correlation between TRFL and age (p =0.0002), and Hb (p=0.044). There was no correlation of telomere length with other factors. Conclusions: Our study showed that TDT patients had shorter telomere length compared with controls. Telomere shortening in TDT was an aging-dependent process and associated with lower hemoglobin level.

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Telomere Length in Patients with Transfusion-Dependent Thalassemia

10.21203/rs.2.17677/v2 ◽

2020 ◽

Author(s):

Nithita Nanthatanti ◽

Adisak Tantiworawit ◽

Pokpong Piriyakhuntorn ◽

Thanawat Rattanathammethee ◽

Sasinee Hantrakool ◽

...

Keyword(s):

Iron Overload ◽

Telomere Length ◽

Major Complication ◽

Cross Sectional Study ◽

Cellular Damage ◽

Repair System ◽

Cross Sectional ◽

Chronic Anemia ◽

Real Time Quantitative Pcr ◽

End Caps

Abstract Background: Thalassemia is a hereditary hemolytic anemia with a severity ranging from mild, non-transfusion dependent to severe chronic anemia requiring lifelong transfusion. Transfusional iron overload is a major complication in patients with transfusion-dependent thalassemia (TDT). Telomeres are sequences of nucleotides forming the end caps of chromosomes that act as a DNA repair system. Iron overload in thalassemia can cause increased oxidative stress which leads to cellular damage and senescence. This may result in telomere length shortening. The degree of telomere length shortening may reflect the severity of thalassemia. Methods: This research aimed to study the telomere length in patients with TDT in comparison to non-thalassemic individuals and to identify the clinical and laboratory parameters that are associated with telomere length. We conducted a cross-sectional study in patients with TDT aged ³18 years. Telomere length was measured by real-time quantitative PCR. Results: Sixty-five patients with TDT were enrolled onto the study. There were 37 female patients (54.4%). The median age was 27 (18-57) years, and mean pre-transfusion hemoglobin (Hb) was 7.1 (± 1.07) g/dL. The mean telomere terminal restriction fragment (TRF) length of patients with TDT and the controls was 6.11 (± 0.61) kb and 6.79 (± 0.84) kb, respectively (p <0.0001). There was a significant correlation between telomere TRF length and age (p =0.0002), and Hb (p=0.044). There was no correlation of telomere length with other factors. Conclusions: Our study showed that TDT patients had shorter telomere length compared with controls. Telomere shortening in TDT was an aging-dependent process and associated with lower hemoglobin level.

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Telomere Length in Patients with Transfusion-Dependent Thalassemia

10.21203/rs.2.17677/v3 ◽

2020 ◽

Author(s):

Nithita Nanthatanti ◽

Adisak Tantiworawit ◽

Pokpong Piriyakhuntorn ◽

Thanawat Rattanathammethee ◽

Sasinee Hantrakool ◽

...

Keyword(s):

Iron Overload ◽

Telomere Length ◽

Major Complication ◽

Single Copy ◽

Cross Sectional Study ◽

Hemoglobin Level ◽

Cellular Damage ◽

Repair System ◽

Leukocyte Telomere Length ◽

Cross Sectional

Abstract Background: Thalassemia is a hereditary hemolytic anemia with a severity ranging from mild, non-transfusion dependent to severe chronic anemia requiring lifelong transfusion. Transfusional iron overload is a major complication in patients with transfusion-dependent thalassemia (TDT). Telomeres are sequences of nucleotides forming the end caps of chromosomes that act as a DNA repair system. Iron overload in thalassemia can cause increased oxidative stress which leads to cellular damage and senescence. This may result in telomere length shortening. The degree of telomere length shortening may reflect the severity of thalassemia. Methods: This research aimed to study the leukocyte telomere length in patients with TDT in comparison to non-thalassemic individuals and to identify the clinical and laboratory parameters that are associated with telomere length. We conducted a cross-sectional study in patients with TDT aged ≥18 years. Leukocyte telomere length was measured by real-time quantitative PCR. Results: Sixty-five patients with TDT were enrolled onto the study. There were 37 female patients (54.4%). The median age was 27 (18-57) years, and mean pre-transfusion hemoglobin level was 7.1±1.07 g/dL. The mean telomere to single copy gene (T/S) ratios of patients with TDT and the controls were 0.72±0.18 and 0.99±0.25, respectively (p <0.0001). There was a significant correlation between the T/S ratio and age (p = 0.0002), and hemoglobin level (p = 0.044). There was no correlation between telomere length and other factors. Conclusions: Our study showed that TDT patients had shorter leukocyte telomere length compared with controls. Leukocyte telomere shortening in TDT was an aging-dependent process and associated with lower hemoglobin level.

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Leukocyte Telomere Length in Patients with Transfusion-Dependent Thalassemia

10.21203/rs.2.17677/v4 ◽

2020 ◽

Author(s):

Nithita Nanthatanti ◽

Adisak Tantiworawit ◽

Pokpong Piriyakhuntorn ◽

Thanawat Rattanathammethee ◽

Sasinee Hantrakool ◽

...

Keyword(s):

Iron Overload ◽

Telomere Length ◽

Major Complication ◽

Single Copy ◽

Cross Sectional Study ◽

Hemoglobin Level ◽

Cellular Damage ◽

Repair System ◽

Leukocyte Telomere Length ◽

Cross Sectional

Abstract Background: Thalassemia is a hereditary hemolytic anemia with a severity ranging from mild, non-transfusion dependent to severe chronic anemia requiring lifelong transfusion. Transfusional iron overload is a major complication in patients with transfusion-dependent thalassemia (TDT). Telomeres are sequences of nucleotides forming the end caps of chromosomes that act as a DNA repair system. Iron overload in thalassemia can cause increased oxidative stress which leads to cellular damage and senescence. This may result in telomere length shortening. The degree of telomere length shortening may reflect the severity of thalassemia. Methods: This research aimed to study the leukocyte telomere length in patients with TDT in comparison to non-thalassemic individuals and to identify the clinical and laboratory parameters that are associated with telomere length. We conducted a cross-sectional study in patients with TDT aged ³18 years. Leukocyte telomere length was measured by real-time quantitative PCR. Results: Sixty-five patients with TDT were enrolled onto the study. There were 37 female patients (54.4%). The median age was 27 (18-57) years, and mean pre-transfusion hemoglobin level was 7.1 (± 1.07) g/dL. The mean telomere to single copy gene (T/S) ratios of patients with TDT and the controls were 0.72±0.18 and 0.99±0.25, respectively (p <0.0001). There was a significant correlation between the T/S ratio and age (p = 0.0002), and hemoglobin level (p = 0.044). There was no correlation between telomere length and other factors. Conclusions: Our study showed that TDT patients had shorter leukocyte telomere length compared with controls. Leukocyte telomere shortening in TDT was an aging-dependent process and associated with lower hemoglobin level.

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Leukocyte telomere length is associated with iron overload in male adults with hereditary hemochromatosis

Bioscience Reports ◽

10.1042/bsr20201916 ◽

2020 ◽

Vol 40 (10) ◽

Author(s):

Maximilino Martín ◽

Andrea Millan ◽

Florencia Ferraro ◽

Walter F. Tetzlaff ◽

Ezequiel Lozano Chiappe ◽

...

Keyword(s):

Iron Overload ◽

Telomere Length ◽

Linear Trend ◽

Iron Status ◽

Hereditary Hemochromatosis ◽

Cellular Aging ◽

Cellular Damage ◽

Cross Sectional ◽

Ferritin Concentration ◽

Telomere Attrition

Abstract Background: Hereditary hemochromatosis (HH) is a primary iron overload (IO) condition. Absolute telomere length (ATL) is a marker of cellular aging and DNA damage associated with chronic diseases and mortality. Aim: To evaluate the relationship between ATL and IO in patients with HH. Methods: Cross-sectional study including 25 patients with HH: 8 with IO and 17 without IO (ferritin < 300 ng/ml) and 25 healthy controls. Inclusion criteria were: age > 18 years, male sex and HH diagnosis. Patients with diabetes or other endocrine and autoimmune diseases were excluded. ATL was measured by real-time PCR. Results: HH patients with IO were older (P<0.001) and showed higher ferritin concentration (P<0.001). Patients with HH, disregarding the iron status, showed higher glucose and body mass index (BMI) than controls (both P<0.01). ATL was shorter in patients with IO than controls [with IO: 8 (6–14), without IO: 13 (9–20), and controls: 19 (15–25) kilobase pairs, P<0.01]; with a linear trend within groups (P for trend <0.01). Differences in ATL remained statistically significant after adjusting by age, BMI and glucose (P<0.05). Discussion: Patients with IO featured shorter ATL while patients without IO showed only mild alterations vs. controls. Screening for IO is encouraged to prevent iron-associated cellular damage and early telomere attrition.

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MANAGEMENT OF BLADDER STONE WITH HOLMIUM-YAG LASER AT KARDINAH HOSPITAL TEGAL

Indonesian Journal of Urology ◽

10.32421/juri.v27i2.473 ◽

2020 ◽

Vol 27 (1) ◽

pp. 1-4

Author(s):

Fatan Abshari ◽

Zulfikar Ali

Keyword(s):

General Hospital ◽

Laser Energy ◽

Major Complication ◽

Mucosal Injury ◽

Cross Sectional Study ◽

Stone Size ◽

Cross Sectional ◽

Yag Laser ◽

Bladder Stones ◽

Large Size

Objective: Transurethral lithotripsy using Holmium-YAG laser has been reported to be beneficial in breaking up bladder stones with large size (>4cm in diameter) with lower risk of mucosal injury and hematuria. The aim of this study is to evaluate the utilization of Holmium-YAG laser for the management of bladder stones at Kardinah General Hospital, Tegal. Material & Methods: This is a cross-sectional study conducted from January 2017 to March 2017. Patient’s demography, which included age, sex, length of surgery, stone size, and laser’s energy count were recorded. Results: We included 120 patients in this study. Mean of patients age in this study was 51.93 years old with age range were 41-85 years old. Most of the patients were male (109 vs 11) with a mean size of stone 25.09 ± 3.04 mm. Length of surgery ranges from 15 to 75 minutes and mean energy of the laser 28.99 ± 19.34 kJ. There was 100% stone’s clearance following surgery with no major complication occurred. Conclusion: Holmium-YAG laser is effective in managing bladder stones at Kardinah General Hospital particularly for large size stones. Length of surgery and energy of laser used depend on the stone size in which bigger stone size is associated with longer surgery time and bigger laser energy needed.

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Cardiac iron overload evaluation in thalassaemic patients using T2* magnetic resonance imaging following chelation therapy: a multicentre cross-sectional study

Hematology Transfusion and Cell Therapy ◽

10.1016/j.htct.2021.01.014 ◽

2021 ◽

Author(s):

Eduardo Cerello Chapchap ◽

Murilo Marques Almeida Silva ◽

Reijane Alves de Assis ◽

Lucila Nassif Kerbauy ◽

Michelli da Silva Diniz ◽

...

Keyword(s):

Magnetic Resonance Imaging ◽

Magnetic Resonance ◽

Iron Overload ◽

Chelation Therapy ◽

Cross Sectional Study ◽

Sectional Study ◽

Resonance Imaging ◽

Cross Sectional ◽

Cardiac Iron ◽

Cardiac Iron Overload

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Molecular Detection of Soil-Transmitted Helminths and Enteric Protozoa Infection in Children and Its Association with Household Water and Sanitation in Manhiça District, Southern Mozambique

Pathogens ◽

10.3390/pathogens10070838 ◽

2021 ◽

Vol 10 (7) ◽

pp. 838

Author(s):

Berta Grau-Pujol ◽

Inocencia Cuamba ◽

Chenjerai Jairoce ◽

Anelsio Cossa ◽

Juliana Da Silva ◽

...

Keyword(s):

Control Program ◽

Cross Sectional Study ◽

P Value ◽

Cross Sectional ◽

Water And Sanitation ◽

Real Time Quantitative Pcr ◽

Protozoan Infection ◽

Household Water ◽

Protozoan Infections ◽

Enteric Protozoa

Intestinal parasite infections can have detrimental health consequences in children. In Mozambique, soil-transmitted helminth (STH) infections are controlled through mass drug administration since 2011, but no specific control program exists for enteric protozoa. This study evaluates STH and protozoan infections in children attending healthcare in Manhiça district, Southern Mozambique, and its association with water and sanitation conditions. We conducted a cross-sectional study in children between 2 and 10 years old in two health centers (n = 405). A stool sample and metadata were collected from each child. Samples were analyzed by multi-parallel real-time quantitative PCR (qPCR). We fitted logistic regression-adjusted models to assess the association between STH or protozoan infection with household water and sanitation use. Nineteen percent were infected with at least one STH and 77.5% with at least one enteric protozoon. qPCR detected 18.8% of participants with intestinal polyparasitism. Protected or unprotected water well use showed a higher risk for at least one protozoan infection in children (OR: 2.59, CI: 1.01–6.65, p-value = 0.010; OR: 5.21, CI: 1.56–17.46, p-value = 0.010, respectively) compared to household piped water. A high proportion of children had enteric protozoan infections. Well consumable water displayed high risk for that.

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Viral Profiling Identifies Multiple Subtypes of Kaposi’s Sarcoma

mBio ◽

10.1128/mbio.01633-14 ◽

2014 ◽

Vol 5 (5) ◽

Cited By ~ 38

Author(s):

Mina C. Hosseinipour ◽

Kristen M. Sweet ◽

Jie Xiong ◽

Dan Namarika ◽

Albert Mwafongo ◽

...

Keyword(s):

Kaposi's Sarcoma ◽

Kaposi’S Sarcoma ◽

Complete Information ◽

The United States ◽

Cross Sectional Study ◽

Cross Sectional ◽

Reading Frame ◽

Real Time Quantitative Pcr ◽

Treatment Naïve ◽

First Time

ABSTRACTKaposi’s sarcoma (KS), caused by KS-associated herpesvirus (KSHV), is the most common cancer among HIV-infected patients in Malawi and in the United States today. In Malawi, KSHV is endemic. We conducted a cross-sectional study of patients with HIV infection and KS with no history of chemo- or antiretroviral therapy (ART). Seventy patients were enrolled. Eighty-one percent had T1 (advanced) KS. Median CD4 and HIV RNA levels were 181 cells/mm3and 138,641 copies/ml, respectively. We had complete information and suitable plasma and biopsy samples for 66 patients. For 59/66 (89%) patients, a detectable KSHV load was found in plasma (median, 2,291 copies/ml; interquartile range [IQR], 741 to 5,623). We utilized a novel KSHV real-time quantitative PCR (qPCR) array with multiple primers per open reading frame to examine KSHV transcription. Seventeen samples exhibited only minimal levels of KSHV mRNAs, presumably due to the limited number of infected cells. For all other biopsy samples, the viral latency locus (LANA, vCyc, vFLIP, kaposin, and microRNAs [miRNAs]) was transcribed abundantly, as was K15 mRNA. We could identify two subtypes of treatment-naive KS: lesions that transcribed viral RNAs across the length of the viral genome and lesions that displayed only limited transcription restricted to the latency locus. This finding demonstrates for the first time the existence of multiple subtypes of KS lesions in HIV- and KS-treatment naive patients.IMPORTANCEKS is the leading cancer in people infected with HIV worldwide and is causally linked to KSHV infection. Using viral transcription profiling, we have demonstrated the existence of multiple subtypes of KS lesions for the first time in HIV- and KS-treatment-naive patients. A substantial number of lesions transcribe mRNAs which encode the viral kinases and hence could be targeted by the antiviral drugs ganciclovir or AZT in addition to chemotherapy.

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