scholarly journals Competitive endogenous RNA network reveals that miR-1343 play key regulatory roles in longissimus dorsi muscle between Saba and Yorkshire pigs

2019 ◽  
Author(s):  
Wei Hu ◽  
Qiang Chen ◽  
Xiaoyi Wang ◽  
Xinxing Dong ◽  
Mingli Li ◽  
...  
Keyword(s):  
Regulatory Mechanism ◽  
Longissimus Dorsi ◽  
Pork Quality ◽  
Regulation Mechanism ◽  
Pig Breeds ◽  
Longissimus Dorsi Muscle ◽  
Dorsi Muscle ◽  
Muscle Formation ◽  
Yorkshire Pigs ◽  
Quality Formation

Abstract Background Pork quality is an important economic trait in pig industry, and increasing data shows that Chinese indigenous and Western commercial pig breeds have significant differences in pork quality. However, the molecular regulatory mechanism of pork quality formation is still unclear. The aim of this study was to explore the transcriptional regulation mechanism of pork quality formation of Chinese Saba pig (SS) and Western Yorkshire pig (YY) breeds. Results A total of 31 DEmiRNAs, 902 DEmRNAs, 47 DElncRNAs and 198 DEcircRNAs were identified in longissimus dorsi muscles between SSs and YYs. On the basis of differentially expressed RNAs, two ceRNA networks including lncRNA-miRNA-mRNA and circRNA-miRNA-mRNA were established, and two ceRNA networks showed that miR-1343 had the most links in two ceRNA networks, which demonstrated that miR-1343 might play key regulatory roles in longissimus dorsi muscle formation between SS and YY breeds. Conclusions The present study identified miR-1343 as key regulatory factor in pig longissimus dorsi muscle formation by ceRNA networks, which provides novel insights into molecular regulatory mechanism of pork quality formation between fat-type and lean-type pig breeds.

scholarly journals Expression Changes of Caveolin Family Genes in Longissimus Dorsi Muscle and Back Subcutaneous Fat of Two Pig Breeds

2012 ◽  
Vol 11 (1) ◽  
pp. 78-82 ◽  
Author(s):  
Yiren Gu ◽  
Yan Liang ◽  
Xiaohui Chen ◽  
Xuemei Yang ◽  
Xuan Tao ◽  
...  
Keyword(s):  
Subcutaneous Fat ◽  
Longissimus Dorsi ◽  
Pig Breeds ◽  
Longissimus Dorsi Muscle ◽  
Dorsi Muscle

scholarly journals PORK QUALITY ATTRIBUTES: THEIR ESTIMATION AND THEIR RELATIONSHIPS WITH CARCASS COMPOSITION IN COMMERCIAL PIGS

1981 ◽  
Vol 61 (2) ◽  
pp. 289-298 ◽  
Author(s):  
A. H. MARTIN ◽  
H. T. FREDEEN ◽  
P. J. L’HIRONDELLE ◽  
A. C. MURRAY ◽  
G. M. WEISS
Keyword(s):  
Regional Differences ◽  
Quality Attributes ◽  
Carcass Composition ◽  
Muscle Quality ◽  
Longissimus Dorsi ◽  
Pork Quality ◽  
Regression Equations ◽  
Longissimus Dorsi Muscle ◽  
Dorsi Muscle ◽  
Wide Range

A survey of certain muscle parameters was conducted on 3114 swine carcasses at three major Canadian commercial packing plants and from a wide range in carcass fatness (backfat measurements) and carcass weights. Sex of carcass had no significant effect on pork quality measurements. There were large regional differences in the incidence of pale, soft, exudative (PSE) and dark, firm, dry (DFD) musculature and these may have resulted from differences in method of stunning (CO2 vs. electrical) or from differences in preslaughter stress and other management conditions associated with plant of origin. Muscle quality attributes were not associated with grade (ie., backfat and weight) and multiple regression equations based on several measures of carcass composition explained less than 11% of the variance observed in any of the quality attributes. The frequency of moderate to severely watery longissimus dorsi muscle (PSE score < 2.0) ranged from 2.5 to 9.7% depending on plant of origin, with plant differences in the frequency of DFD musculature (scores > 3.5) ranging from 21.5 to 34.9%. Muscle temperature and pH taken 45 min postmortem, the measures conventionally employed for early detection of potentially PSE pork, were of negligible utility for this purpose.

scholarly journals Transcriptome analysis of differential gene expression in the longissimus dorsi muscle from Debao and landrace pigs based on RNA-sequencing

2019 ◽  
Vol 39 (12) ◽  
Author(s):  
Shang-Qiao Song ◽  
Wei-wei Ma ◽  
Su-Xian Zeng ◽  
Chao-Long Zhang ◽  
Jin Yan ◽  
...  
Keyword(s):  
Reference Genome ◽  
Longissimus Dorsi ◽  
Pork Quality ◽  
Muscular Tissue ◽  
Longissimus Dorsi Muscle ◽  
Qrt Pcr ◽  
Dorsi Muscle ◽  
Pig Genome ◽  
Key Genes ◽  
The Difference

Abstract RNA-seq analysis was used to identify differentially expressed genes (DEGs) at the genetic level in the longissimus dorsi muscle from two pigs to investigate the genetic mechanisms underlying the difference in meat quality between Debao pigs and Landrace pigs. Then, these DEGs underwent functional annotation, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment, and protein–protein interaction (PPI) analyses. Finally, the expression levels of specific DEGs were assessed using qRT-PCR. The reference genome showed gene dosage detection of all samples which showed that the total reference genome comprised 22342 coding genes, including 14743 known and 190 unknown genes. For detection of the Debao pig genome, we obtained 14168 genes, including 13994 known and 174 unknown genes. For detection of the Landrace pig genome, we obtained 14404 genes, including 14223 known and 181 unknown genes. GO analysis and KEGG signaling pathway analysis show that DEGs are significantly related to metabolic regulation, amino acid metabolism, muscular tissue, muscle structure development etc. We identified key genes in these processes, such as FOS, EGR2, and IL6, by PPI network analysis. qRT-PCR confirmed the differential expression of six selected DEGs in both pig breeds. In conclusion, the present study revealed key genes and related signaling pathways that influence the difference in pork quality between these breeds and could provide a theoretical basis for improving pork quality in future genetic thremmatology.

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