Mitochondrial function and oxidative stress markers in higher-frequency episodic migraine

Abstract Introduction Increasing evidence points towards the role of mitochondrial functioning, energy metabolism, and oxidative stress in migraine. However not all previous research has been conclusive and some mitochondrial function / oxidative stress markers have not yet been examined. Methods To this end, alpha-lipoic acid (ALA), total thiols, total plasma antioxidant capacity (TAC), lipid peroxide (PerOx), oxidised LDL (oxLDL), HbA1c and lactate were determined in the serum of 32 higher frequency episodic migraineurs (5-14 migraine days/ months, 19 with aura, 28 females) in this cross-sectional study. Results The majority of patients had abnormally low ALA and lactate levels (87.5% and 78.1%, respectively). 46.9% of the patients had abnormally high PerOx values, while for thiols and TAC over one third of patients had abnormally low values (31.2% and 37.5%, respectively). 21.9% of patients had abnormally low HbA1c and none had an HbA1c level above 5.6%. oxLDL was normal in all but one patient. Discussion This study provides further evidence for a role of oxidative stress and altered metabolism in migraine pathophysiology, which might represent a suitable therapeutic target. ALA, being too low in almost 90% of patients, might represent a potential biomarker for migraine. Further research is needed to replicate these results, in particular a comparison with a control group. This study is part of the trial registration: ClinicalTrials.gov: NCT03132233, registered on 27.04.2017, https://clinicaltrials.gov/ct2/show/NCT03132233

Download Full-text

Mitochondrial function and oxidative stress markers in higher-frequency episodic migraine

Scientific Reports ◽

10.1038/s41598-021-84102-2 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Elena C. Gross ◽

Niveditha Putananickal ◽

Anna-Lena Orsini ◽

Deborah R. Vogt ◽

Peter S. Sandor ◽

...

Keyword(s):

Oxidative Stress ◽

Mitochondrial Function ◽

Lipid Peroxide ◽

Control Group ◽

Oxidative Stress Markers ◽

Stress Markers ◽

Potential Biomarker ◽

Plasma Antioxidant Capacity ◽

And Oxidative Stress

AbstractIncreasing evidence points towards the role of mitochondrial functioning, energy metabolism, and oxidative stress in migraine. However not all previous research has been conclusive and some mitochondrial function/oxidative stress markers have not yet been examined. To this end, alpha-lipoic acid (ALA), total thiols, total plasma antioxidant capacity (TAC), lipid peroxide (PerOx), oxidised LDL (oxLDL), HbA1c and lactate were determined in the serum of 32 higher frequency episodic migraineurs (5–14 migraine days/ months, 19 with aura, 28 females) in this cross-sectional study. The majority of patients had abnormally low ALA and lactate levels (87.5% and 78.1%, respectively). 46.9% of the patients had abnormally high PerOx values, while for thiols and TAC over one third of patients had abnormally low values (31.2% and 37.5%, respectively). 21.9% of patients had abnormally low HbA1c and none had an HbA1c level above 5.6%. oxLDL was normal in all but one patient. This study provides further evidence for a role of oxidative stress and altered metabolism in migraine pathophysiology, which might represent a suitable therapeutic target. ALA, being too low in almost 90% of patients, might represent a potential biomarker for migraine. Further research is needed to replicate these results, in particular a comparison with a control group.This study is part of the trial registration: ClinicalTrials.gov: NCT03132233, registered on 27.04.2017, https://clinicaltrials.gov/ct2/show/NCT03132233.

Download Full-text

Evaluation of Salivary Antioxidants and Oxidative Stress Markers in Type 2 Diabetes Mellitus: A Retrospective Cohort Study

Endocrine Metabolic & Immune Disorders - Drug Targets ◽

10.2174/1871530319666191016103222 ◽

2020 ◽

Vol 20 (4) ◽

pp. 584-590 ◽

Cited By ~ 1

Author(s):

Shima Fathi ◽

Shiva Borzouei ◽

Mohammad Taghi Goodarzi ◽

Jalal Poorolajal ◽

Fatemeh Ahmadi-Motamayel

Keyword(s):

Oxidative Stress ◽

Diabetes Mellitus ◽

Control Group ◽

Oxidative Stress Markers ◽

Stress Markers ◽

Type 2 Dm ◽

Patients With Diabetes ◽

The Difference ◽

And Oxidative Stress

Background: Diabetes Mellitus (DM) is a progressive metabolic disorder. Objective: The aim of this study was to investigate the relationship between antioxidant and oxidative stress markers in the saliva of patients with type 2 DM and a healthy control group. Methods: In this study, 20 patients with diabetes and 20 healthy individuals were evaluated. Salivary antioxidants markers consisted of total antioxidant capacity (TAC), uric acid (UA), peroxidase and catalase. Oxidative stress markers included total oxidant status (TOS), malondealdehyde (MDA) and total thiol (SH). Sialochemical analysis was performed with spectrophotometric assay. All the statistical analyses were conducted using STATA software. Results: TAC decreased significantly in patients with diabetes. Although salivary UA and peroxidase were lower in patients with diabetes compared to the control group, the difference was not significant. Salivary catalase in patients with diabetes was significantly lower than that in the control group. MDA and TOS exhibited significantly higher levels in type 2 DM. SH levels were slightly higher in DM. Conclusions: According to the results of the present study, there were some changes in the salivary levels of some antioxidants and oxidative stress markers in patients with type 2 DM and could be measured as an indicator of serum changes..

Download Full-text

ROLE OF VITAMIN D AND OXIDATIVE STRESS MARKERS IN THE DEVELOPMENT AND PROGRESSION OF TYPE 2 DIABETES MELLITUS

International Journal of Biology Pharmacy and Allied Sciences ◽

10.31032/ijbpas/2020/9.4.4978 ◽

2020 ◽

Vol 9 (4) ◽

Keyword(s):

Oxidative Stress ◽

Diabetes Mellitus ◽

Type 2 Diabetes ◽

Vitamin D ◽

Type 2 Diabetes Mellitus ◽

Oxidative Stress Markers ◽

Stress Markers ◽

And Oxidative Stress

Download Full-text

Correlation between the Activity of Aldehyde Dehydrogenase and Oxidative Stress Markers in the Saliva of Diabetic Patients

Protein and Peptide Letters ◽

10.2174/0929866526666191002115121 ◽

2019 ◽

Vol 27 (1) ◽

pp. 67-73

Author(s):

Hina Younus ◽

Sumbul Ahmad ◽

Md. Fazle Alam

Keyword(s):

Oxidative Stress ◽

Aldehyde Dehydrogenase ◽

Early Stage ◽

Control Group ◽

Diabetic Patients ◽

Oxidative Stress Markers ◽

Stress Markers ◽

Patients With Diabetes ◽

Markers Of Oxidative Stress ◽

And Oxidative Stress

Background: Reactive aldehydes are involved in diseases associated with oxidative stress, including diabetes. Human salivary aldehyde dehydrogenase (hsALDH) presumably protects us from many toxic ingredient/contaminant aldehydes present in food. Objective: This study aimed to probe the activity of hsALDH in patients with diabetes and than to correlate it with various oxidative stress markers in the saliva. Methods: The saliva samples were collected from total 161 diabetic patients from Rajiv Gandhi Centre for Diabetes, Jawaharlal Nehru Medical College (JNMC), AMU, Aligarh, (India). HsALDH activity and markers of oxidative stress [8-hydroxydeoxyguanosine (8-OHDG), malondialdehyde (MDA) and advanced glycation end products (AGEs)] were measured in the saliva samples. Results: Patients with early stage of diabetes had higher activity of hsALDH when compared with the control group. As the history of diabetes increases, the activity of the enzyme decreases and also higher oxidative stress markers (8-OHDG, MDA and AGEs) are detected in the saliva samples. Negative significant correlation between hsALDH activity and oxidative stress markers were observed (p <0.0001). Conclusion: The activity of hsALDH increases in early stages of diabetes most probably to counter the increased oxidative stress associated with diabetes. However, in later stages of diabetes, the activity of the enzyme decreases, possibly due to its inactivation resulting from glycation.

Download Full-text

“2-(4-Fluorobenzamido)-4-methylthiazole-5-carboxylic acid” a novel thiazole compound, ameliorates insulin sensitivity and hyperlipidaemia in streptozotocin-induced diabetic rats: Plausible role of inflammatory and oxidative stress markers

Biomedicine & Pharmacotherapy ◽

10.1016/j.biopha.2017.09.014 ◽

2017 ◽

Vol 95 ◽

pp. 1232-1241 ◽

Cited By ~ 1

Author(s):

Yam Nath Paudel ◽

Md. Rahmat Ali ◽

Mohammad Adil ◽

Sandhya Bawa ◽

Manju Sharma

Keyword(s):

Oxidative Stress ◽

Insulin Sensitivity ◽

Carboxylic Acid ◽

Diabetic Rats ◽

Oxidative Stress Markers ◽

Stress Markers ◽

And Oxidative Stress

Download Full-text

ANTIHYPERGLYCEMIC AND ANTIOXIDATIVE EFFECTS OF LYGODIUM MICROPHYLLUM IN ALLOXAN INDUCED DIABETIC RATS

International Journal of Pharmacy and Pharmaceutical Sciences ◽

10.22159/ijpps.2018v10i12.29232 ◽

2018 ◽

Vol 10 (12) ◽

pp. 75

Author(s):

D. G. Syahidah Nadiah Binti Abdull Majid ◽

Mohammad Iqbal

Keyword(s):

Oxidative Stress ◽

Body Weight ◽

Blood Glucose ◽

Fasting Blood Glucose ◽

Diabetic Rats ◽

Control Group ◽

Oxidative Stress Markers ◽

Stress Markers ◽

Antioxidative Effects ◽

And Oxidative Stress

Objective: The antihyperglycemic and antioxidative effects of L. microphyllum were evaluated by using in vivo methods in normal and alloxan induced diabetic rats.Methods: Diabetes was induced in Sprague Dawley rats by injecting alloxan through intravenous (i. v) at a dose of 100 mg/kg of body weight. Aqueous extract of L. microphyllum at different doses (400, 200 and 100 mg/kg of body weight) was administered orally (orogastric intubation) for 14 d. Blood glucose and oxidative stress markers were measured. Hematoxylin and eosin staining method were used to examine the pancreatic tissues.Results: At the 14 d interval, fasting blood glucose showed a reduction in serum glucose levels in animals pretreated with L. microphyllum compared with alloxan alone treated group. Oxidative stress was noticed in rat’s pancreatic tissue as evidenced by a significant decrease in glutathione level, glutathione reductase, glutathione-S-transferase, and catalase activities. Malondialdehyde showed a significant increase compared to the normal saline-treated control group. Serum biochemistry and oxidative stress markers were consistent with the pancreatic histopathological studies. Treatment of diabetic rats with L. microphyllum at a dose level of 100, 200 and 400 mg/kg body weight leaves extract for 14 d significantly prevented these alterations and attenuated alloxan-induced oxidative stress (P<0.05).Conclusion: The results of the present study indicated that the antihyperglycemic potential of L. microphyllum might be ascribable to its antioxidant and free radical scavenging properties. Thus, it is concluded that L. microphyllum may be helpful in the prevention of diabetic complications associated with oxidative stress.

Download Full-text

The role of nickel (Ni) and drought in serpentine adaptation: contrasting effects of Ni on osmoprotectants and oxidative stress markers in the serpentine endemic, Cleome heratensis, and the related non-serpentinophyte, Cleome foliolosa

Plant and Soil ◽

10.1007/s11104-017-3250-9 ◽

2017 ◽

Vol 417 (1-2) ◽

pp. 183-195 ◽

Cited By ~ 5

Author(s):

Behrooz Salehi Eskandari ◽

Seyed Majid Ghaderian ◽

Henk Schat

Keyword(s):

Oxidative Stress ◽

Oxidative Stress Markers ◽

Stress Markers ◽

Cleome Heratensis ◽

And Oxidative Stress

Download Full-text

Rosuvastatin Affects Tracheal Responsiveness, Bronchoalveolar Lavage Inflammatory Cells, and Oxidative Stress Markers in Hyperlipidemic and Asthmatic Rats

Iranian Journal of Allergy Asthma and Immunology ◽

10.18502/ijaai.v18i6.2175 ◽

2020 ◽

Author(s):

Saeideh Saadat ◽

Amin Mokhtari-Zaer ◽

Mousa-Al-Reza Hadjzadeh ◽

Mohammad Hossein Boskabady

Keyword(s):

Oxidative Stress ◽

Superoxide Dismutase ◽

Bronchoalveolar Lavage ◽

Control Group ◽

Oxidative Stress Markers ◽

Total Thiol ◽

Stress Markers ◽

Thiol Content ◽

Wbc Count ◽

And Oxidative Stress

Statins provide greater protection than predicted from just cholesterol-lowering effects, which is possibly mediated by other pleiotropic actions. This study aimed to examine the possible interaction effect of asthma on lipid profiles and evaluate the effect of rosuvastatin treatment on asthma. The animals were assigned into (1) control, (2) asthmatic, (3) hyperlipidemic, (4) asthmatic-hyperlipidemic, (5) rosuvastatin (40 mg/kg/day intraperitoneally, for 3 weeks)-treated asthmatic, (6) rosuvastatin-treated hyperlipidemic and (7) rosuvastatin-treated asthmatic-hyperlipidemic groups. Tracheal responsiveness to methacholine and ovalbumin, total and differential WBC (white blood cell) counts, and oxidative stress markers in bronchoalveolar lavage fluid (BALF) were evaluated. In the asthmatic and asthmatic-hyperlipidemic groups, tracheal responsiveness to ovalbumin, total WBC count, numbers of eosinophils, neutrophils, and monocytes were higher than the control group (p<0.001). A left-ward shift in the concentration-response curves to methacholine, an increase in nitrite and malondialdehyde concentrations, and a decrease in total thiol content, superoxide dismutase and catalase activities were also observed in the asthmatic and asthmatic-hyperlipidemic groups compared to control group (p<0.01 to p<0.001). Beyond lipid-lowering effect in the treated hyperlipidemic and asthmatic-hyperlipidemic groups, rosuvastatin treatment decreased tracheal responsiveness to methacholine, reduced total WBC count, the numbers of eosinophils, neutrophils, and monocytes, as well as decreased malondialdehyde concentration, and increased total thiol content, superoxide dismutase and catalase activities in treated asthmatic and asthmatic-hyperlipidemic groups (p<0.05 to p<0.001). The improving effect of rosuvastatin on asthmatic and asthmatic-hyperlipidemic animals was shown due to pleiotropic mechanisms including the effect on airway hyperresponsiveness, lung inflammation, and oxidative stress.

Download Full-text