scholarly journals The fabrication of the complex bio-fertilizer for wheat cultivation based on collection bacteria of the PGPR group

2020 ◽  
Vol 21 (11) ◽  
Author(s):  
RAKHILYA AIPOVA ◽  
AIZHAN ABDYKADYROVA ◽  
DMITRY SILAYEV ◽  
ERKIN TAZABEKOVA ◽  
IRINA OSHERGINA ◽  
...  

Abstract. Aipova R, Abdykadyrova A, Silayev D, Tazabekova E, Oshergina I, Ten E, Kurmanbaye A. 2020. The fabrication of the complex bio-fertilizer for wheat cultivation based on collection bacteria of the PGPR group. Biodiversitas 21: 5021-5028. The development of new types of biological products based on microbial complexes from local bacterial strains is a great theoretical and practical interest for agriculture. It can provide an opportunity for better preservation of the natural properties of the wheat products under extreme conditions. The aim of this study was to obtain and test a biological product to increase wheat productivity in northern Kazakhstan. Our data indicate the potential of Plant Growth-Promoting Rhizobacteria (PGPR) group bacteria for the development of biofertilizers and biopesticides. For instance, the bacteria B. mojavensis showed effectiveness in the experiments with the wheat (Astana-2 type). We observed an increase in wheat yield by 15% under conditions of artificial infection of crops with snow mold (by 2.5fold compared with the control). The results demonstrated that the B. mojavensis Lhv 97 strain can be used as an ingredient of biological products due to its activity against plant diseases caused by phytopathogenic fungi.

2020 ◽  
Author(s):  
Wei-Liang Kong ◽  
Pu-Sheng Li ◽  
Xiao-Qin Wu ◽  
Tian-Yu Wu ◽  
Xiao-Rui Sun

Abstract Plant growth-promoting rhizobacteria can potentially be used as an alternative strategy to control plant disease. In this study, strain ST-TJ4 isolated from the rhizosphere soil of a healthy poplar was found to have strong antifungal activity against 11 phytopathogenic fungi in agriculture and forestry. Strain ST-TJ4 was identified as Pseudomonas chlororaphis subsp. aurantiaca based on 16S rDNA sequences. The bacterium can produce siderophores, cellulase, and protease, and has genes involved in the synthesis of phenazine, 1-phenazinecarboxylic acid, pyrrolnitrin, and hydrogen cyanide. Moreover, the volatile compounds released by strain ST-TJ4 can inhibit the mycelial growth of plant pathogenic fungi more than diffusible substances can. Based on volatile compound profiles of strain ST-TJ4 obtained from headspace collection and GC-MS/MS analysis, 1-undecene was identified. In summary, the results suggested that P. chlororaphis subsp. aurantiaca ST-TJ4 can be used as a biocontrol agent for various plant diseases caused by phytopathogenic fungi.


2020 ◽  
Vol 8 (4) ◽  
pp. 590 ◽  
Author(s):  
Wei-Liang Kong ◽  
Pu-Sheng Li ◽  
Xiao-Qin Wu ◽  
Tian-Yu Wu ◽  
Xiao-Rui Sun

Plant growth-promoting rhizobacteria (PGPR) can potentially be used as an alternative strategy to control plant diseases. In this study, strain ST–TJ4 isolated from the rhizosphere soil of a healthy poplar was found to have a strong antifungal activity against 11 phytopathogenic fungi in agriculture and forestry. Strain ST–TJ4 was identified as Pseudomonas sp. based on 16S rRNA-encoding gene sequences. The bacterium can produce siderophores, cellulase, and protease, and has genes involved in the synthesis of phenazine, 1–phenazinecarboxylic acid, pyrrolnitrin, and hydrogen cyanide. Additionally, the volatile compounds released by strain ST–TJ4 can inhibit the mycelial growth of plant pathogenic fungi more than diffusible substances can. Based on volatile compound profiles of strain ST–TJ4 obtained from headspace collection and GC–MS/MS analysis, 1-undecene was identified. In summary, the results suggested that Pseudomonas sp. ST–TJ4 can be used as a biocontrol agent for various plant diseases caused by phytopathogenic fungi.


2020 ◽  
Vol 2 (4) ◽  
pp. 36-41
Author(s):  
R. Aipova ◽  
A. B. Abdykadyrova ◽  
A. A. Kurmanbayev

The review presents data on the creation of complex microbial preparations and their application in agricultural practice. According to economists, the turnover in the field of organic agriculture is worth 85-90 billion US dollars a year. Developers of biological products pay great attention to the creation of complex biofertilizers, which contribute to a stable 20-25% increase in yield, with a significant reduction of plant damage by root rot. Among the considered positive effects of plant growth promoting rhizobacteria (PGPR) on plants are the ability to fix molecular nitrogen from the atmosphere, the synthesis of hormonal and fungitoxic substances, and the mobilization of sparingly soluble soil phosphates. The presented data show promise for the use of these microorganisms in the development of eco-friendly farming technologies in order to increase plant productivity and establish biocontrol over the development of plant diseases, reduce the chemical load on the soil, and increase its fertility.


Proceedings ◽  
2021 ◽  
Vol 66 (1) ◽  
pp. 27
Author(s):  
Rim Tinhinen Maougal ◽  
Maya Kechid ◽  
Chaima Ladjabi ◽  
Abdelhamid Djekoun

Rhizobacteria play an important role in maintaining soil balance. Among these bacteria, there are those taht have shown their ability to promote the growth of plants, known as Plant Growth Promoting Rhizobacteria (PGPR). In our work, we are interested in characterizing 110 bacterial strains isolated in the field in the region of Ben Badis (Constantine Algeria) from 5 varieties of faba bean. Phenotypic and biochemical characterization showed that most of the isolates are cream-colored, slightly raised, flat and opaque, Gram−, catalase+ and oxidase−, and Bacillus form. PCA analysis allowed us to select 40 isolates with a high degree of variability to continue our work. The results obtained have directed us towards different taxonomic groups (rhizobium, Pseudomonas, Bacillus etc.). The evaluation of the PGPR potential of bacteria (phytostimulation, biofertilization and biocontrol), showed that 100% of bacteria are able to produce auxin at different concentrations, with the highest concentration (177.77 µg/mL) for the isolate 6, and that more than 50% of isolates are capable of producing nitrogen, ammonia and phytate mineralization. These PGPR traits have a direct effect on plant growth of five varieties of the faba bean and can be used to select the best performing bacteria for inoculation tests.


2020 ◽  
pp. 1186-1194
Author(s):  
Roberta Mendes dos Santos ◽  
Everlon Cid Rigobelo

The search for plant growth-promoting rhizobacteria is an ongoing need for the development of new bioinoculants for use in various crops, including sugarcane. Bacterial strains with various plant growth-promoting properties can contribute to sustainable agricultural production. The present study aimed to isolate, characterize and select sugarcane rhizobacteria from six different varieties through principal components analysis. This study selected 167 bacterial strains with the ability to fix nitrogen, produce indolacetic acid, exhibit cellulolytic activity, and solubilize phosphate and potassium were isolated. Of these 167 bacterial strains, seven were selected by principal component analysis and identified as belonging to the genera Staphylococcus, Enterobacter, Bacillus and Achromobacter. Bacillus thuringiensis IP21 presented higher potential for nitrogen fixation and CaPO4 and AlPO4 solubilization and a lower potential for K solubilization in sugarcane. Enterobacter asburiae IP24 was efficient in indolacetic acid production and CaPO4 and FePO4 solubilization and inefficient for Araxá apatite solubilization.


Author(s):  
L. S. Veselova ◽  
◽  
G. V. Mirskaya ◽  
Yu. V. Ostankova ◽  
T. A. Kuznetsova ◽  
...  

The aim of this study is to search for promising strains of bacteria to create new biological products. New bacteria were isolated from wheat grains and identified by 16 S rRNA sequencing as Bacillus sp. L1 and Panaebacillus sp. L2. The cytokinin-producing bacteria Bacillussp.L 1 increased the length of the roots of plants by 70 %, the mass of roots by 17 %. N-fixing Panaebacillus sp. L2 increased the root length by 39 % and the mass by 27 %. The results of vegetation experiments on growing wheat of the ‘Leningradskaya 6’ variety under controlled conditions showed an increase in wheat productivity by 15.6 % when inoculated with Bacillus sp. L1 and by 31.2 % when inoculated with Panaebacillus sp. L2. Preliminary results of vegetative experiments indicate that Bacillus sp. L1 and Panaebacillus sp. L2 can be used as an active components of biological products to increase wheat productivity.


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