scholarly journals Seroprevalence and molecular characteristics of varicella-zoster virus infection in Chinese children

2019 ◽  
Author(s):  
Lin Luan ◽  
Xiaochen Shen ◽  
Jing Qiu ◽  
Yang Jing ◽  
Jingqi Zhang ◽  
...  
Keyword(s):  
Varicella Zoster Virus ◽  
Varicella Vaccine ◽  
Public Health Problem ◽  
Chinese Children ◽  
Varicella Vaccination ◽  
Enzyme Linked Immunosorbent Assay ◽  
Molecular Characteristics ◽  
Varicella Zoster Virus Infection ◽  
Detection Rates ◽  
Varicella Zoster

Abstract Background Varicella-zoster virus (VZV) infection in children is an important public health problem in China. We performed the current study to explore the seroprevalence of VZV infection in Chinese children in order to provide more information for improvement of varicella vaccination in China. Methods 3014 serum samples were collected from Chinese kindergarten students aged from four to six years. Anti-VZV IgG and IgM were assayed using enzyme-linked immunosorbent assay. Both ORF22 and ORF62 of VZV were amplified, sequenced, and analyzed by nested PCR. Results Totally, 43.9% of boys and 46.3% of girls were vaccinated with varicella vaccine, respectively. The seroprevalence of anti-VZV IgG was 54.4% in the children with varicella vaccination, which was significantly higher than those in unvaccinated children (49.2%) (χ2=8.206, P=0.004). Among of the vaccinated children, the detection rates of VZV IgG antibody increased with age, with 49.4%, 50.9% and 58.9% in 4, 5 and 6-year groups, respectively (Trend χ2=17.202, P=0.002). However, there was no difference in anti-VZV IgG detection rates among those unvaccinated children in different age groups (Trend χ2=8.681, P=0.070). In addition, thirteen boys and 13 girls were positive for anti-VZV IgM, respectively. Among of them, eight children (0.6%) have received varicella vaccination, which was similar to those in unvaccinated children (1.1%). However, only one ORF22 sequence was isolated from an unvaccinated 5-year boy. Compared to the reference VZV sequences, the nucleotide homology was estimated to be 99.7% with genotype J. Conclusions Our study indicated that about half of Chinese children aged four to six years have a high risk of VZV infection. It should be helpful for the evaluation on the necessity of varicella immunization in China.

scholarly journals Seroprevalence and molecular characteristics of varicella-zoster virus infection in Chinese children

2019 ◽  
Author(s):  
Lin Luan ◽  
Xiaochen Shen ◽  
Jing Qiu ◽  
Yang Jing ◽  
Jingqi Zhang ◽  
...  
Keyword(s):  
Varicella Vaccine ◽  
Public Health Problem ◽  
Chinese Children ◽  
Varicella Vaccination ◽  
Enzyme Linked Immunosorbent Assay ◽  
Molecular Characteristics ◽  
Varicella Zoster Virus Infection ◽  
Igg Antibody ◽  
Important Public Health Problem ◽  
Detection Rates

Abstract Background:Varicella-zoster virus (VZV) infection in children is an important public health problem in China. We performed the current study to explore the seroprevalence of VZV infection in Chinese children in order to provide more information for improvement of varicella vaccination in China. Methods: 3014 children were recruited from Chinesekindergarten students aged from four to six years.Anti-VZVIgG and IgM were assayed using enzyme-linked immunosorbent assay. BothORF22and ORF62 of VZV were amplified, sequenced, and analyzed by nested PCR. Results:Among 3014 children,43.9% of boys and 46.3% of girls were vaccinated with varicella vaccine, respectively. The seroprevalence of anti-VZV IgG was 54.4% in the children with varicella vaccination, which was significantly higher than those in unvaccinated children (49.2%) (χ2=8.206, P=0.004). Among of the vaccinated children, the detection rates of VZV IgG antibody increased with age, with 49.4%, 50.9% and 58.9% in 4, 5 and 6-year groups, respectively (Trend χ2=17.202, P=0.002). However, there was no difference in anti-VZV IgG detection rates among those unvaccinated children in different age groups (Trend χ2=8.681, P=0.070). In addition, thirteen boys and 13 girls were positive for anti-VZV IgM, respectively. Among of them, eight children (0.6%) have received varicella vaccination, which was similar to those in unvaccinated children (1.1%). However, only one ORF22 sequence was isolated from an unvaccinated 5-year boy. Compared to the reference VZV sequences, the nucleotide homology was estimated to be 99.7% with genotype J. Conclusions:Our study indicated that about half of Chinese children aged four to six years have a high risk of VZV infection. It should be helpful for the evaluation on the necessity of varicella immunization in China.

scholarly journals Seroprevalence and molecular characteristics of varicella-zoster virus infection in Chinese children

2019 ◽  
Author(s):  
Lin Luan ◽  
Xiaochen Shen ◽  
Jing Qiu ◽  
Yang Jing ◽  
Jingqi Zhang ◽  
...  
Keyword(s):  
Varicella Vaccine ◽  
Public Health Problem ◽  
Chinese Children ◽  
Varicella Vaccination ◽  
Enzyme Linked Immunosorbent Assay ◽  
Molecular Characteristics ◽  
Varicella Zoster Virus Infection ◽  
Igg Antibody ◽  
Important Public Health Problem ◽  
Detection Rates

Abstract Background:Varicella-zoster virus (VZV) infection in children is an important public health problem in China. We performed the current study to explore the seroprevalence of VZV infection in Chinese children in order to provide more information for improvement of varicella vaccination in China. Methods: 3014 children were recruited from Chinesekindergarten students aged from four to six years.Anti-VZVIgG and IgM were assayed using enzyme-linked immunosorbent assay. BothORF22and ORF62 of VZV were amplified, sequenced, and analyzed by nested PCR. Results:Among 3014 children,43.9% of boys and 46.3% of girls were vaccinated with varicella vaccine, respectively. The seroprevalence of anti-VZV IgG was 54.4% in the children with varicella vaccination, which was significantly higher than those in unvaccinated children (49.2%) (χ2=8.206, P=0.004). Among of the vaccinated children, the detection rates of VZV IgG antibody increased with age, with 49.4%, 50.9% and 58.9% in 4, 5 and 6-year groups, respectively (Trend χ2=17.202, P=0.002). However, there was no difference in anti-VZV IgG detection rates among those unvaccinated children in different age groups (Trend χ2=8.681, P=0.070). In addition, thirteen boys and 13 girls were positive for anti-VZV IgM, respectively. Among of them, eight children (0.6%) have received varicella vaccination, which was similar to those in unvaccinated children (1.1%). However, only one ORF22 sequence was isolated from an unvaccinated 5-year boy. Compared to the reference VZV sequences, the nucleotide homology was estimated to be 99.7% with genotype J. Conclusions:Our study indicated that about half of Chinese children aged four to six years have a high risk of VZV infection. It should be helpful for the evaluation on the necessity of varicella immunization in China.

scholarly journals Prevention & Management of Herpes Zoster- an Update

2014 ◽  
Vol 41 (3) ◽  
pp. 53-56
Author(s):  
AKM Rejaul Haque ◽  
A Sultana ◽  
A Habib ◽  
ASM Zakaria
Keyword(s):  
Herpes Zoster ◽  
Virus Infection ◽  
Varicella Zoster Virus ◽  
Age Factors ◽  
Varicella Vaccine ◽  
Varicella Zoster Virus Infection ◽  
Post Herpetic Neuralgia ◽  
Varicella Zoster ◽  
Burning Pain ◽  
Chronic Corticosteroid

Herpes zoster (commonly referred to as "shingles") results .from reactivation of the varicella-zoster virus infection, or chickenpox. Were as varicella is generally a disease of childhood, herpes zoster becomes more common with increasing age Factors that decrease immune function, such as human immunodeficiency virus infection, chemotherapv, malignancies and chronic corticosteroid use, may also increase the risk of developing herpes zoster. Reactivation of latent varicella-zoster virus from dorsal root ganglia is responsible.for lhe classic dermatomal rash and pain that occur with herpes zoster. Burning pain typically precedes the rash by several days and can persist for several months after the rash resolves. With post herpetic neuralgia, a complication of herpes zoster, pain may persist well after resolution of the rash and can be highly debilitating. Although the diagnosis of the conditions is generally straightforward, treatment can be frustrating for the patient and physician. Approaches to management include treatment of the herpes zoster infection and associated pain, prevention of post herpetic neuralgia, and control of the neuropathic pain until the condition resolves. Herpes zoster is contagious to those who have not had varicella or have not received the varicella vaccine. The role of the varicella vaccine in preventing herpes zoster is uncertain, but is being studied. The management of herpes zoster is challenging because many patients develop troublesome complication. So, appropriate management o/'herpes zoster is very important to avoid complication. On the other hand prevention is better than cure. Immunization with varicella zoster virus vaccine may boost humoral and cell mediated and decrease the incidence of zoster in population. So effectiveness of a vaccination program need to be evaluated. immunity DOI: http://dx.doi.org/10.3329/bmj.v41i3.18961 Bangladesh Medical Journal 2012 Vol.41(3): 53-56

scholarly journals Detection of Antibodies to Varicella-Zoster Virus in Recipients of the Varicella Vaccine by Using a Luciferase Immunoprecipitation System Assay

2014 ◽  
Vol 21 (9) ◽  
pp. 1288-1291 ◽  
Author(s):  
Jeffrey I. Cohen ◽  
Mir A. Ali ◽  
Ahmad Bayat ◽  
Sharon P. Steinberg ◽  
Hosun Park ◽  
...  
Keyword(s):  
Varicella Zoster Virus ◽  
High Throughput ◽  
Fluorescent Antibody ◽  
Varicella Vaccine ◽  
The United States ◽  
Antibody Detection ◽  
Enzyme Linked Immunosorbent Assay ◽  
Viral Capsid Antigen ◽  
Glycoprotein E ◽  
Varicella Zoster

ABSTRACTA high-throughput test to detect varicella-zoster virus (VZV) antibodies in varicella vaccine recipients is not currently available. One of the most sensitive tests for detecting VZV antibodies after vaccination is the fluorescent antibody to membrane antigen (FAMA) test. Unfortunately, this test is labor-intensive, somewhat subjective to read, and not commercially available. Therefore, we developed a highly quantitative and high-throughput luciferase immunoprecipitation system (LIPS) assay to detect antibody to VZV glycoprotein E (gE). Tests of children who received the varicella vaccine showed that the gE LIPS assay had 90% sensitivity and 70% specificity, a viral capsid antigen enzyme-linked immunosorbent assay (ELISA) had 67% and 87% specificity, and a glycoprotein ELISA (not commercially available in the United States) had 94% sensitivity and 74% specificity compared with the FAMA test. The rates of antibody detection by the gE LIPS and glycoprotein ELISA were not statistically different. Therefore, the gE LIPS assay may be useful for detecting VZV antibodies in varicella vaccine recipients. (This study has been registered at ClinicalTrials.gov under registration no. NCT00921999.)

scholarly journals Varicella Zoster Antibodies after Herpes Zoster, Varicella and Multiple Sclerosis

1997 ◽  
Vol 24 (2) ◽  
pp. 137-139 ◽  
Author(s):  
R.T. Ross ◽  
L.E. Nicolle ◽  
M.R. Dawood ◽  
Mary Cheang ◽  
C. Feschuk
Keyword(s):  
Herpes Zoster ◽  
Virus Infection ◽  
Varicella Zoster Virus ◽  
Population Group ◽  
Population Based ◽  
Postal Code ◽  
Enzyme Linked Immunosorbent Assay ◽  
Varicella Zoster Virus Infection ◽  
Varicella Zoster ◽  
Physician Care

ABSTRACT:Background:We previously showed that Manitoba Hutterites seek physician care for varicella zoster virus infection significantly less than non-Hutterites. The current study was undertaken to measure varicella zoster virus seroprevalence for Hutterite and non-Hutterite controls.Methods:Blood was obtained from 315 Hutterites and 259 similar age and sex controls at the time of blood donations to The Canadian Red Cross Society. The controls were from the same or a contiguous postal code area and were collected at the same time as the Hutterite samples. The immune status of the specimens was determined by the ELISA method (enzyme linked immunosorbent assay).Results:Twenty-eight per cent of 315 Hutterites had no immunity and an additional 25% had only marginal immunity. Among the 259 controls, 10% had no immunity and an additional 10% had only marginal immunity (p < .0001).Conclusions:Manitoba Hutterites have significantly decreased seroprevalence to varicella zoster virus infection. This study of serum varicella zoster virus antibodies verifies a previous population based study that demonstrated the relative rarity of varicella and herpes zoster among a particular population group.

scholarly journals Seroepidemiology of varicella-zoster virus in Korean adolescents and adults using fluorescent antibody to membrane antigen test

2014 ◽  
Vol 143 (8) ◽  
pp. 1643-1650 ◽  
Author(s):  
S. B. HAN ◽  
K. R. KANG ◽  
D. H. HUH ◽  
H. C. LEE ◽  
J. H. KIM ◽  
...  
Keyword(s):  
Varicella Zoster Virus ◽  
Fluorescent Antibody ◽  
Age Groups ◽  
Varicella Vaccination ◽  
Enzyme Linked Immunosorbent Assay ◽  
Study Cohort ◽  
Cross Sectional ◽  
Entire Study ◽  
Varicella Zoster ◽  
Adolescents And Adults

SUMMARYWe conducted a cross-sectional seroepidemiological study in 2012–2013 to determine the seroprevalence of varicella-zoster virus (VZV) in adolescents and adults living in Korea, where varicella vaccination has been recommended universally at age 12–15 months since 2005. Residual serum samples were collected from 1196 healthy adults and adolescents aged ⩾10 years between November 2012 and March 2013. The fluorescent antibody to membrane antigen (FAMA) test and enzyme-linked immunosorbent assay (ELISA) were performed to determine the seroprevalence of VZV. The seroprevalences of VZV were compared between six age groups: 10–19, 20–29, 30–39, 40–49, 50–59, and ⩾60 years. The seroprevalence of VZV in the entire study cohort was 99·1% according to the FAMA test and 93·1% as determined by ELISA. The seroprevalences of the six age groups were as follows: 96·0%, 99·5%, 99·5%, 99·5%, 100%, and 100%, respectively, by the FAMA test, and 83·3%, 93·0%, 93·0%, 97·5%, 94·5%, and 97·5%, respectively, by ELISA. Seroprevalence increased significantly with age (P< 0·001); moreover, the seroprevalence in subjects aged 10–19 years was significantly lower than in other age groups (P< 0·001), as measured by both the FAMA test and ELISA. Thus, strategies to increase protective immunity against VZV in teenagers are necessary.

scholarly journals Validity of Self-Reported Varicella Disease History in Pregnant Women Attending Prenatal Clinics

2007 ◽  
Vol 122 (4) ◽  
pp. 499-506 ◽  
Author(s):  
Barbara Watson ◽  
Rachel Civen ◽  
Meredith Reynolds ◽  
Karl Heath ◽  
Dana Perella ◽  
...  
Keyword(s):  
Pregnant Women ◽  
Strong Predictor ◽  
Varicella Vaccine ◽  
Routine Care ◽  
Varicella Vaccination ◽  
Enzyme Linked Immunosorbent Assay ◽  
Igg Antibodies ◽  
Serological Evidence ◽  
Varicella Zoster ◽  
Cell Enzyme

Objective. The purpose of this study was to assess the validity of self-reported history for varicella disease relative to serological evidence of varicella immunity in pregnant women attending antenatal care at clinics located in two diverse geographical locations in the U.S. (Antelope Valley, California, and Philadelphia) with high varicella vaccination coverage. Methods. Pregnant women attending prenatal care appointments who needed blood drawn as part of their routine care were eligible to participate. Self-reported varicella disease history was obtained via questionnaire. Varicella serostatus was determined using a whole-cell enzyme-linked immunosorbent assay to test for varicella zoster virus-specific immunoglobulin G (VZV IgG) antibodies. Results. Of the 309 study participants from Antelope Valley and the 528 participants from Philadelphia who self-reported having had chickenpox disease, 308 (99.7%; 95% confidence interval [CI]: 98.2, 100) and 517 (97.9%; 95% CI: 96.3, 99.0), respectively, had serological evidence of immunity to varicella. Only 6.8% (95% CI: 3.9, 11.0) and 17.4% (95% CI: 13.1, 22.5) of women who self-reported having a negative or uncertain varicella disease history in Antelope Valley and Philadelphia, respectively, were seronegative for varicella antibodies. Conclusion. Despite the dramatic changes in the epidemiology of varicella that have occurred since 1995 due to the introduction and subsequent widespread use of the varicella vaccine, self-reported history of varicella continues to be a strong predictor of VZV IgG antibodies in pregnant women. Negative or uncertain history remains poorly predictive of negative serostatus.

Immunoglobulin Subclass Antibodies to Varicefla-Zoster Virus

PEDIATRICS ◽  
1987 ◽  
Vol 80 (6) ◽  
pp. 933-936
Author(s):  
Yoshizo Asano ◽  
Yuichi Hiroishi ◽  
Naoko Itakura ◽  
Shigeyuki Hirose ◽  
Yuji Kajita ◽  
...  
Keyword(s):  
Varicella Zoster Virus ◽  
Natural Infection ◽  
Varicella Vaccine ◽  
Enzyme Linked Immunosorbent Assay ◽  
Antibody Activity ◽  
Igg Subclass ◽  
Igg Antibody ◽  
Varicella Zoster ◽  
Immunoglobulin Subclass ◽  
Igg4 Antibody

Commercially available mouse monoclonal antibodies to human IgG subclass (IgG1 to IgG4) were applied to an enzyme-linked immunosorbent assay to measure IgG subclass-specific antibodies to varicellazoster virus in children naturally infected with varicellazoster virus and in varicella vaccine recipients. In children naturally infected with varicella-zoster virus, IgG 1 antibody was detected 2 weeks after onset of the disease in all cases, its activity increased at 1 month after onset, and almost equal antibody value was maintained 10 years after infection. This pattern of antibody response was similar to that of total IgG antibody to varicella-zoster virus after natural infection. On the other hand, low antibody activity was found in IgG2 only at 1 month of the disease. The highest antibody level of IgG3 was shown 2 weeks after onset of the disease; then, it gradually decreased, and no antibody activity was detected 10 years later. IgG4 antibody was first detected 1 month after onset and an almost equal level of antibody was shown 10 years after the disease. After inoculation of children with a live varicella vaccine, in contrast, IgG subclass antibody responses to vaccine recipients were almost equal to those after natural infection.

scholarly journals Prevalence of IgG varicella zoster virus antibodies in the Kuikuro and Kaiabi indigenous communities in Xingu National Park, Brazil, before varicella vaccination

2005 ◽  
Vol 47 (3) ◽  
pp. 139-142 ◽  
Author(s):  
Manuel Mindlin Lafer ◽  
Maria Isabel de Moraes-Pinto ◽  
Lily Yin Weckx
Keyword(s):  
Varicella Zoster Virus ◽  
National Park ◽  
Varicella Vaccine ◽  
Indigenous Communities ◽  
Varicella Vaccination ◽  
Varicella Infection ◽  
Predictive Values ◽  
Varicella Zoster ◽  
Vaccine Introduction ◽  
History Of

The purpose of the study was to estimate the prevalence of IgG antibodies against varicella zoster virus (VZV) in the two most populated indigenous ethnic groups from Xingu Indigenous National Park, in Brazil, prior to the introduction of vaccination against the disease, and to determine the positive and the negative predictive values of a history of varicella infection. In 2001, 589 inhabitants of two Kuikuro villages and three Kaiabi villages were evaluated and provided information concerning previous varicella infection. An indirect immunosorbent assay (ELISA) to detect IgG anti-VZV antibodies was performed in 224 blood samples - volunteer selection had no interference of anamnesis. IgG prevalence was 80.8% (95% Confidence Interval: 76% - 86%). The seroepidemiology of varicella in Xingu National Park prior to varicella vaccine introduction was comparable to the Brazilian national seroprevalence described in the literature, and so were the positive (98%) and the negative predictive value (41%) of the referred history.

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