scholarly journals Paenibacillus Polymyxa MVY-024: Plant Growth Promoting Bacteria Which is Easy to Apply on an Industrial Scale

2021 ◽  
Author(s):  
Justina Kaziūnienė ◽  
Raimonda Mažylytė ◽  
Aurimas Krasauskas ◽  
Monika Toleikienė ◽  
Audrius Gegeckas
Keyword(s):  
Cultured Cells ◽  
Root Nodules ◽  
Air Flow ◽  
Nitrogen Sources ◽  
Paenibacillus Polymyxa ◽  
Nitrogen Accumulation ◽  
Plant Growth Promoting Bacteria ◽  
Mineral N ◽  
Rdna Sequences ◽  
Plant Growth Promoting

Abstract In this study, thirteen isolates which were possibly expected to fix nitrogen, were isolated from the soil and pea root nodules and identified by gene analysis of 16S rDNA sequences. Two of these isolates which were able to form endospores and growth on nitrogen free media were selected for spring wheat development research. The isolate Paenibacillus sp. S7 identified as Paenibacillus polymyxa was found to significantly increased amount of ammonium and mineral N amounts in the soil. Furthermore, increased nitrogen accumulation in grain and a chlorophyll index were obtained after wheat treatment. Paenibacillus sp. S7 isolate was selected for further studies and accession number MT900581 and strain name MVY-024 in NCBI nucleotide bank for this isolate was assigned. During cultivation of Paenibacillus sp. MVY-024, sugarcane molasses and yeast extract were determined as the most suitable carbon and nitrogen sources, optimal concentrations were 100 gL-1 and 10 gL-1, respectively. The optimal pH range for cells culturing was between 6.5 and 7.0, optimal air flow rate was 0.4 vvm. It was found that air flow has effect for biomass production and cells endospores formation. After Paenibacillus sp. MVY-024 biomass cultivation optimization, cultured cells number was on average 2.2 × 109 cfu mL-1.

scholarly journals Alone Yet Not Alone: Frankia Lives Under the Same Roof With Other Bacteria in Actinorhizal Nodules

2021 ◽  
Vol 12 ◽  
Author(s):  
Faten Ghodhbane-Gtari ◽  
Timothy D’Angelo ◽  
Abdellatif Gueddou ◽  
Sabrine Ghazouani ◽  
Maher Gtari ◽  
...  
Keyword(s):  
16S Rrna ◽  
Plant Growth ◽  
16S Rrna Gene ◽  
Root Nodules ◽  
Actinorhizal Plants ◽  
Rrna Gene ◽  
Plant Growth Promoting Bacteria ◽  
Metagenomic Sequencing ◽  
Plant Growth Promoting ◽  
Growth Promoting

Actinorhizal plants host mutualistic symbionts of the nitrogen-fixing actinobacterial genus Frankia within nodule structures formed on their roots. Several plant-growth-promoting bacteria have also been isolated from actinorhizal root nodules, but little is known about them. We were interested investigating the in planta microbial community composition of actinorhizal root nodules using culture-independent techniques. To address this knowledge gap, 16S rRNA gene amplicon and shotgun metagenomic sequencing was performed on DNA from the nodules of Casuarina glauca. DNA was extracted from C. glauca nodules collected in three different sampling sites in Tunisia, along a gradient of aridity ranging from humid to arid. Sequencing libraries were prepared using Illumina NextEra technology and the Illumina HiSeq 2500 platform. Genome bins extracted from the metagenome were taxonomically and functionally profiled. Community structure based off preliminary 16S rRNA gene amplicon data was analyzed via the QIIME pipeline. Reconstructed genomes were comprised of members of Frankia, Micromonospora, Bacillus, Paenibacillus, Phyllobacterium, and Afipia. Frankia dominated the nodule community at the humid sampling site, while the absolute and relative prevalence of Frankia decreased at the semi-arid and arid sampling locations. Actinorhizal plants harbor similar non-Frankia plant-growth-promoting-bacteria as legumes and other plants. The data suggests that the prevalence of Frankia in the nodule community is influenced by environmental factors, with being less abundant under more arid environments.

scholarly journals Paenibacillus polymyxa Invades Plant Roots and Forms Biofilms

2005 ◽  
Vol 71 (11) ◽  
pp. 7292-7300 ◽  
Author(s):  
Salme Timmusk ◽  
Nina Grantcharova ◽  
E. Gerhart H. Wagner
Keyword(s):  
Plant Growth ◽  
Fluorescent Protein ◽  
Paenibacillus Polymyxa ◽  
Plant Roots ◽  
Root Tip ◽  
Broad Host Range ◽  
Plant Growth Promoting Bacteria ◽  
Soil System ◽  
Plant Growth Promoting ◽  
Growth Promoting

ABSTRACT Paenibacillus polymyxa is a plant growth-promoting rhizobacterium with a broad host range, but so far the use of this organism as a biocontrol agent has not been very efficient. In previous work we showed that this bacterium protects Arabidopsis thaliana against pathogens and abiotic stress (S. Timmusk and E. G. H. Wagner, Mol. Plant-Microbe Interact. 12:951-959, 1999; S. Timmusk, P. van West, N. A. R. Gow, and E. G. H. Wagner, p. 1-28, in Mechanism of action of the plant growth promoting bacterium Paenibacillus polymyxa, 2003). Here, we studied colonization of plant roots by a natural isolate of P. polymyxa which had been tagged with a plasmid-borne gfp gene. Fluorescence microscopy and electron scanning microscopy indicated that the bacteria colonized predominantly the root tip, where they formed biofilms. Accumulation of bacteria was observed in the intercellular spaces outside the vascular cylinder. Systemic spreading did not occur, as indicated by the absence of bacteria in aerial tissues. Studies were performed in both a gnotobiotic system and a soil system. The fact that similar observations were made in both systems suggests that colonization by this bacterium can be studied in a more defined system. Problems associated with green fluorescent protein tagging of natural isolates and deleterious effects of the plant growth-promoting bacteria are discussed.

scholarly journals Identification of Canola Roots Endophytic Bacteria and Analysis of Their Potential as Biofertilizers for Canola Crops with Special Emphasis on Sporulating Bacteria

Agronomy ◽  
2021 ◽  
Vol 11 (9) ◽  
pp. 1796
Author(s):  
Pilar Martínez-Hidalgo ◽  
José David Flores-Félix ◽  
Fernando Sánchez-Juanes ◽  
Raúl Rivas ◽  
Pedro F. Mateos ◽  
...  
Keyword(s):  
Endophytic Bacteria ◽  
Paenibacillus Polymyxa ◽  
Plant Growth Promoting Bacteria ◽  
Microcosm Experiment ◽  
Brassica Napus L ◽  
Bacillus Simplex ◽  
Paenibacillus Sp ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Hydroponic Conditions

Canola (Brassica napus L. var. oleracea) is the third most common oil-producing crop worldwide after palm and soybean. Canola cultivation requires the use of chemical fertilizers, but the amount required can be reduced by applying plant growth-promoting bacteria (PGPB). Among PGPB, endophytic bacteria have certain advantages as biofertilizers, but canola endophytic bacteria have rarely been studied. In this work, we identified a collection of bacterial endophytes isolated from canola roots using MALDI-TOF MS, a technique that is still rarely used for the identification of such bacteria, and rrs gene sequencing, a methodology that is commonly used to identify canola endophytes. The results demonstrated that some bacterial isolates from canola roots belonged to the genera Bacillus, Neobacillus, Peribacillus (Pe.), and Terribacillus, but most isolates belonged to the genera Paenibacillus (P.) and Pseudomonas (Ps.). Inoculation of these isolates indicated that several of them could efficiently promote canola seedling growth in hydroponic conditions. These results were then confirmed in a microcosm experiment using agricultural soil, which demonstrated that several isolates of Pseudomonas thivervalensis, Paenibacillus amylolyticus, Paenibacillus polymyxa, Paenibacillus sp. (Paenibacillus glucanolyticus/Paenibacillus lautus group), and Peribacillus simplex (previously Bacillus simplex) could efficiently promote canola shoot growth under greenhouse conditions. Among them, the isolates of Paenibacillus and Peribacillus were the most promising biofertilizers for canola crops as they are sporulated rods, which is an advantageous trait when formulating biofertilizers.

Isolation of plant-growth-promotingBacillusstrains from soybean root nodules

2002 ◽  
Vol 48 (3) ◽  
pp. 230-238 ◽  
Author(s):  
Yuming Bai ◽  
Frederic D'Aoust ◽  
Donald L Smith ◽  
Brian T Driscoll
Keyword(s):  
Bacillus Subtilis ◽  
Bacillus Thuringiensis ◽  
Plant Growth ◽  
Endophytic Bacteria ◽  
Root Nodules ◽  
Rrna Gene ◽  
Plant Growth Promoting Bacteria ◽  
Plant Growth Promoting ◽  
Glycine Max L ◽  
Promote Plant Growth

Endophytic bacteria reside within plant tissues and have often been found to promote plant growth. Fourteen strains of putative endophytic bacteria, not including endosymbiotic Bradyrhizobium strains, were isolated from surface-sterilized soybean (Glycine max. (L.) Merr.) root nodules. These isolates were designated as non-Bradyrhizobium endophytic bacteria (NEB). Three isolates (NEB4, NEB5, and NEB17) were found to increase soybean weight when plants were co-inoculated with one of the isolates and Bradyrhizobium japonicum under nitrogen-free conditions, compared with plants inoculated with B. japonicum alone. In the absence of B. japonicum, these isolates neither nodulated soybean, nor did they affect soybean growth. All three isolates were Gram-positive spore-forming rods. While Biolog tests indicated that the three isolates belonged to the genus Bacillus, it was not possible to determine the species. Phylogenetic analysis of 16S rRNA gene hypervariant region sequences demonstrated that both NEB4 and NEB5 are Bacillus subtilis strains, and that NEB17 is a Bacillus thuringiensis strain.Key words: root nodule, endophytic bacteria, plant-growth-promoting bacteria, Bacillus subtilis, Bacillus thuringiensis.

scholarly journals Inoculation with plant growth-promoting bacteria and reduction of nitrogen fertilizer in herbage accumulation and nutritional value of Mavuno grass

2021 ◽  
Vol 9 (3) ◽  
pp. 16-34
Author(s):  
Amário Nuno Meireles Duarte ◽  
Cecílio Viega Soares Filho ◽  
Marcelo Carvalho Minhoto Teixeira Filho ◽  
Caroline Lopes Monteiro de Carvalho ◽  
Mariangela Hungria ◽  
...  
Keyword(s):  
Plant Growth ◽  
Nutritional Value ◽  
Block Design ◽  
Control Treatment ◽  
Plant Growth Promoting Bacteria ◽  
Rhizobium Tropici ◽  
Mineral N ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Insoluble Fiber

Strategies that improve the use of nitrogen (N), by tropical forage grasses, can bring environmental and social gains. Thus, this study aimed to evaluate the forage productivity and the nutritional value (NV) of the hybrid Urochloa spp. cv. ´Mavuno`, under inoculation with plant growth-promoting bacteria (PGPB) and doses of N. The experiment was carried out in the field, for a period of 14 months, in a randomized block design, with nine treatments and four replications in plots of 9 m². Seed inoculations were tested with: Azospirillum brasilense Ab-V5 and Ab-V6 strains; Pseudomonas fluorescens CCTB 03 and co-inoculation with Rhizobium tropici CIAT 899 and A. brasilense Ab-V6, combined with 50 and 100 kg ha-1 of added mineral N; fertilization with N-mineral with these doses, without inoculation and the control treatment, without N and without inoculation. The bacteria were inoculated to the grass seeds. The forage was evaluated by determining the shoot dry weight yield (SDWY) and the levels of crude protein (CP), neutral detergent insoluble fiber (NDF), acid detergent insoluble fiber (ADF) and in vitro dry matter digestibility (IVDMD). The results were subjected to analysis of variance by the F test (p ≤ 0.05) and compared to each other by the Scott-Knott test at 5% probability. Inoculation with PGPB resulted in greater SDWY when associated with a dose of 50 kg ha-1 of N. In plants inoculated with A. brasilense Ab-V5 + Ab-V6 and P. fluorescens CCTB 03, associated with a dose of 50 kg ha-1 of N-mineral, SDWY was similar to that obtained with double the dose of N in the absence of inoculation. The inoculation with PGPB resulted in an increase in the NV of Mavuno grass, with emphasis on A. brasilense Ab-V5 + Ab-V6 and P. fluorescens CCTB 03, associated with the dose of 100 kg ha-1 of N-mineral that improved the nutritional value in all evaluated items. The inoculation with A. brasilense Ab-V5 + Ab-V6 was the most promising, followed by P. fluorescens CCTB 03, mainly in association with 50 kg ha-1 of N.

Isolation and identification of plant growth-promoting bacteria associated with tall fescue

2009 ◽  
pp. 211-216 ◽  
Author(s):  
J. Monk ◽  
E. Gerard ◽  
S. Young ◽  
K. Widdup ◽  
M. O'Callaghan
Keyword(s):  
New Zealand ◽  
Plant Growth ◽  
Tall Fescue ◽  
Festuca Arundinacea ◽  
Plant Growth Promoting Bacteria ◽  
Beneficial Bacteria ◽  
Isolation And Identification ◽  
Naturally Occurring ◽  
Plant Growth Promoting ◽  
Growth Promoting

Tall fescue (Festuca arundinacea) is a useful alternative to ryegrass in New Zealand pasture but it is slow to establish. Naturally occurring beneficial bacteria in the rhizosphere can improve plant growth and health through a variety of direct and indirect mechanisms. Keywords: rhizosphere, endorhiza, auxin, siderophore, P-solubilisation

Effect of plant growth promoting bacteria and drought on spring maize (Zea mays L.)

2020 ◽  
Vol 53 (2) ◽  
Author(s):  
Muhammad Mubeen ◽  
Asghari Bano ◽  
Barkat Ali ◽  
Zia Ul Islam ◽  
Ashfaq Ahmad ◽  
...  
Keyword(s):  
Zea Mays ◽  
Plant Growth ◽  
Zea Mays L ◽  
Plant Growth Promoting Bacteria ◽  
Spring Maize ◽  
Plant Growth Promoting ◽  
Growth Promoting
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