scholarly journals Evaluation of the Potential Effect of Cry1Ab Expressing Straw on ICR Mice

2021 ◽  
Author(s):  
Bo Lv ◽  
Yun-e Tang ◽  
Chao-min Li ◽  
Ou-lin Dai ◽  
Yong Peng ◽  
...  
Keyword(s):  
Rice Straw ◽  
Transgenic Rice ◽  
Sperm Quality ◽  
Day Treatment ◽  
Follicular Development ◽  
Ion Concentration ◽  
Integrated Analysis ◽  
Unintended Effects ◽  
Cry1ab Protein ◽  
Icr Mice

Abstract Cry1Ab toxin has been effectively integrated into crops such as rice and cotton for pest control, and the safety evaluation of transgenic rice has attracted widespread attention. Nevertheless, the effects of transgenic rice straw on animal model are still unclear. Hence, the present study conducted an integrated analysis to evaluate the unintended effects of transgenic rice straw expressing Cry1Ab protein on the Institute of Cancer Research (ICR) mice under 90-day treatment. The results indicated that Cry1Ab rice straw had no significant effects on the behavior and body weight of mice. In addition, physiological indicators, including hemogram, blood biochemistry, apoptosis rate, and calcium ion concentration of the blood lymphocytes, displayed no alterations under Cry1Ab protein stress. Similarly, Cry1Ab rice straw had no adverse effects on several antioxidase activities (i.e., catalase, superoxide dismutase, peroxidase, glutathione peroxidase, and acetylcholine esterase). Moreover, we recorded that Cry1Ab stress did not adversely impact the sperm quality and follicular development of male and female ICR mice. Collectively, this integrated analysis indicates that Cry1Ab rice straw has no adverse or toxic effects on ICR mice after 90-day treatment and provides multi-level perspectives to assess the safety of genetically modified crops on non-target mammals.

scholarly journals Rice straw incorporated just before soil flooding increases acetic acid formation and decreases available nitrogen

2014 ◽  
Vol 38 (1) ◽  
pp. 177-184 ◽  
Author(s):  
Ronaldir Knoblauch ◽  
Paulo Roberto Ernani ◽  
Francisco Carlos Deschamps ◽  
Luciano Colpo Gatiboni ◽  
Timothy Wayne Walker ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Organic Acids ◽  
Soil Solution ◽  
Rice Straw ◽  
Day Treatment ◽  
Ion Concentration ◽  
Ammonium Ion ◽  
Rice Seedlings ◽  
Mineral N ◽  
Soil Flooding

Incorporation of rice straw into the soil just before flooding for water-seeded rice can immobilize mineral nitrogen (N) and lead to the production of acetic acid harmful to the rice seedlings, which negatively affects grain yield. This study aimed to evaluate the formation of organic acids and variation in pH and to quantify the mineral N concentration in the soil as a function of different times of incorporation of rice straw or of ashes from burning the straw before flooding. The experiment was carried out in a greenhouse using an Inceptisol (Typic Haplaquept) soil. The treatments were as follows: control (no straw or ash); incorporation of ashes from previous straw burning; rice straw incorporated to drained soil 60 days before flooding; straw incorporated 30 days before flooding; straw incorporated 15 days before flooding and straw incorporated on the day of flooding. Experimental units were plastic buckets with 6.0 kg of soil. The buckets remained flooded throughout the trial period without rice plants. Soil samples were collected every seven days, beginning one day before flooding until the 13th week of flooding for determination of mineral N- ammonium (NH4+) and nitrate (NO3-). Soil solution pH and concentration of organic acids (acetic, propionic and butyric) were determined. All NO3- there was before flooding was lost in approximately two weeks of flooding, in all treatments. There was sigmoidal behavior for NH4+ formation in all treatments, i.e., ammonium ion concentration began to rise shortly after soil flooding, slightly decreased and then went up again. On the 91st day of flooding, the NH4+ concentrations in soil was 56 mg kg-1 in the control treatment, 72 mg kg-1 for the 60-day treatment, 73 mg kg-1 for the 30-day treatment and 53 mg kg-1 for the ash incorporation treatment. These ammonium concentrations correspond to 84, 108, 110 and 80 kg ha-1 of N-NH4+, respectively. When the straw was incorporated on the day of flooding or 15 days before, the concentration of N-NH4+ in the soil was 28 and 54 mg kg-1, equivalent to an accumulation of 42 and 81 kg ha-1 of N-NH4+, respectively. There was formation of acetic acid in which toxic concentrations were reached (7.2 mmol L-1) on the 15th day of flooding only for the treatment with straw incorporated on the day of flooding. The pH of the soil solution of all the treatments increased after flooding and this increase was faster in the treatments with incorporation of straw, followed by the ash treatment and then the control. After 60 days of flooding, however, the pH values were around 6.5 for all treatments, except for the control, which reached a pH of 6.3. Rice straw should be incorporated into the soil at least 30 days before flooding; otherwise, it may immobilize part of the mineral N and produce acetic acid in concentrations toxic to rice seedlings.

Cry1Ab protein from Bt transgenic rice does not residue in rhizosphere soil

2006 ◽  
Vol 143 (3) ◽  
pp. 449-455 ◽  
Author(s):  
Haiyan Wang ◽  
Qingfu Ye ◽  
Wei Wang ◽  
Licheng Wu ◽  
Weixiang Wu
Keyword(s):  
Transgenic Rice ◽  
Rhizosphere Soil ◽  
Cry1ab Protein ◽  
Bt Transgenic Rice

Protective effect of some selected fruit blends on testicular toxicity in alloxan-induced diabetic rats

2015 ◽  
Vol 12 (2) ◽  
Author(s):  
Okukwe Obode ◽  
Oluwatoyin Okafor ◽  
Ochuko Erukainure ◽  
Atinuke Ajayi ◽  
Yewande Suberu ◽  
...  
Keyword(s):  
Protective Effect ◽  
Antioxidant Activities ◽  
Reduced Glutathione ◽  
Sperm Quality ◽  
Day Treatment ◽  
Quality Parameters ◽  
Diabetic Rats ◽  
Testicular Toxicity ◽  
Cervical Dislocation

Abstract: The protective effect of a developed drink from blends of selected fruits on the sperm quality of alloxan-induced diabetic rats was investigated.: Diabetes was intraperitoneally induced with alloxan; the formulated drink blend was orally administered 2.5 or 5 mL/day. Treatment lasted for 14 days and the rats were humanely sacrificed by cervical dislocation. The antioxidant status via assessment of reduced glutathione (GSH), catalase (CAT), superoxide dismutase (SOD) and lipid peroxidation (LPO) was carried out on the testis, while sperm cells were analyzed for sperm motility, counts and abnormality.: Induction of diabetes led to a significant (p<0.05) decrease in GSH level, elevated SOD and CAT activities, significant (p<0.05) decrease in the sperm quality parameters studied. However, treatment with the formulated drink led to a significant (p<0.05) reduction of LPO, SOD and CAT activities as well as increase in GSH level.: This study shows an improvement in testicular antioxidant activities and sperm qualities by single and double doses of the formulation, suggesting its protective potential against testicular toxicity in diabetic rats.

scholarly journals The Effect of Screening and Treatment of Ureaplasma Urealyticum Infection on Semen Parameters in Asymptomatic Leukocytospermia: A Case Control Study

2020 ◽  
Author(s):  
Qi-Feng Zhang ◽  
Yu-Ji Zhang ◽  
Sheng Wang ◽  
Yu Wei ◽  
Feng Li ◽  
...  
Keyword(s):  
Ureaplasma Urealyticum ◽  
Sperm Quality ◽  
Day Treatment ◽  
Treatment Plan ◽  
Sperm Concentration ◽  
Good Choice ◽  
Semen Parameters ◽  
Adult Males ◽  
Normal Morphology ◽  
Cure Rates

Abstract Background: Ureaplasma urealyticum (UU) infection, as well as asymptomatic leukocytospermia, whether it has effect on semen parameters and whether it needs screening and treatment is still a confusing and controversial topic for clinicians.Methods: Among 1,530 adult males who visited Guilin People's Hospital due to infertility, 295 were diagnosed with asymptomatic leukocytospermia, and 95 were further screened for UU-positive. 81 UU-positive asymptomatic leukocytospermia patients received 7-day or 14-day treatment plan with doxycycline, and 70 cases were cured. The semen parameters of non-leukocytospermia, leukocytospermia, UU-positive leukocytospermia and UU-negative leukocytospermia groups were compared, and the differences between the two treatment plans and the semen parameters before UU treatment and 1 month after UU-cured were compared.Results: Compared with non-leukocytospermia patients, the sperm concentration, progressive motility (PR), and normal morphology of patients with leukocytospermia decreased, while those with UU-positive leukocytospermia performed more significantly. The PR, total motility, and normal morphology of UU-positive leukocytospermia patients were significantly lower than those of UU-negative leukocytospermia patients (all p<0.001). The UU cure rates of the 7-day and 14-day treatment plan with doxcycline was 84.62% and 89.66% (p=0.738), respectively, and the sperm concentration, PR, total motility, and normal morphology of the cured UU-positive leukocytospermia patients were all increased after 1 month (p=0.001, p=0.022, p=0.004 and p=0.008, respectively).Conclusions: It is significant to screen and treat UU infection in asymptomatic leukocytospermia for improving sperm quality. Where appropriate, the 7-day treatment plan with doxycycline may be a good choice.

scholarly journals The effect of screening and treatment of Ureaplasma urealyticum infection on semen parameters in asymptomatic leukocytospermia: a case control study

2020 ◽  
Author(s):  
Qi-Feng Zhang ◽  
Yu-Ji Zhang ◽  
Sheng Wang ◽  
Yu Wei ◽  
Feng Li ◽  
...  
Keyword(s):  
Ureaplasma Urealyticum ◽  
Sperm Quality ◽  
Day Treatment ◽  
Treatment Plan ◽  
Sperm Concentration ◽  
Good Choice ◽  
Semen Parameters ◽  
Adult Males ◽  
Normal Morphology ◽  
Cure Rates

Abstract Background: Ureaplasma urealyticum (UU) infection, as well as asymptomatic leukocytospermia, whether it has effect on semen parameters and whether it needs screening and treatment is still a confusing and controversial topic for clinicians.Methods: Among 1,530 adult males who visited Guilin People's Hospital due to infertility, 295 were diagnosed with asymptomatic leukocytospermia, and 95 were further screened for UU-positive. 81 UU-positive asymptomatic leukocytospermia patients received 7-day or 14-day treatment plan with doxycycline, and 70 cases were cured. The semen parameters of non-leukocytospermia, leukocytospermia, UU-positive leukocytospermia and UU-negative leukocytospermia groups were compared, and the differences between the two treatment plans and the semen parameters before UU treatment and 1 month after UU-cured were compared.Results: Compared with non-leukocytospermia patients, the sperm concentration, progressive motility (PR), and normal morphology of patients with leukocytospermia decreased, while those with UU-positive leukocytospermia performed more significantly. The PR, total motility, and normal morphology of UU-positive leukocytospermia patients were significantly lower than those of UU-negative leukocytospermia patients (all p<0.001). The UU cure rates of the 7-day and 14-day treatment plan with doxycycline was 84.62% and 89.66% (p=0.738), respectively, and the sperm concentration, PR, total motility, and normal morphology of the cured UU-positive leukocytospermia patients were all increased after 1 month (p=0.001, p=0.022, p=0.004 and p=0.008, respectively).Conclusions: It is significant to screen and treat UU infection in asymptomatic leukocytospermia for improving sperm quality. Where appropriate, the 7-day treatment plan with doxycycline may be a good choice.

scholarly journals Transcriptomic and proteomic analyses between small yellow follicles and the smallest hierarchical follicles reveal a role of VLDLR in chicken follicle selection

2019 ◽  
Author(s):  
Qiuyue Chen ◽  
Yiya Wang ◽  
Zemin Liu ◽  
Xiaoli Guo ◽  
Yi Sun ◽  
...  
Keyword(s):  
Laying Hens ◽  
Follicular Development ◽  
Differentially Expressed ◽  
Integrated Analysis ◽  
Functional Genes ◽  
Differentially Expressed Proteins ◽  
Important Process ◽  
Laying Performance ◽  
Follicle Selection

Abstract Background Follicle selection in chicken refers to the process of selecting one from a group of small yellow follicles (SY, 6-8mm in diameter) to enter the 12-15 mm hierarchical follicles (usually F6 follicles), which is a an important process affecting laying performance in the poultry industry. Although transcripromic analysis on chicken ovarian follicles was reported, integrated analysis on chicken follicles around selection by using both transcripromic and proteomic approaches was still lacking. In this study, we compared the proteomes and transcriptomes of SY and F6 follicles of laying hens and found some genes involved in chicken follicle selection.Results Transcriptomic analysis revealed 855 differentially expressed genes (DEGs) between SY follicles and F6 follicles of laying hens, among which 202 were upregulated and 653 were downregulated. Proteomic analysis revealed 259 differentially expressed proteins (DEPs), including 175 upregulated and 84 downregulated proteins. Among the identified DEGs and DEPs, the expression changes of seven genes including VLDLR1,WIF1, NGFR, AMH, BMP15, GDF6 and MMP13 , and nine proteins including VLDLR, VTG1, VTG3, PSCA, APOB, APOV1, F10, ZP2 and ZP3L2 were validated. In addition VLDLR expression was significantly down-regulated in F6 follicles compared with SY follicles, was signifcantly higher in the GCs than in the TCs and was stimulated by FSH in GCs of both hierarchical and prehierarchical follicles.Conclusions By comparing the proteomes and transcriptomes of SY follicles and F6 of laying hens, we identified some differentially expressed proteins/genes that might play certain roles in chicken follicle selection. These data may contribute to identification of the functional genes and proteins involved in chicken follicular development and selection.

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