Protective effect of some selected fruit blends on testicular toxicity in alloxan-induced diabetic rats

2015 ◽  
Vol 12 (2) ◽  
Author(s):  
Okukwe Obode ◽  
Oluwatoyin Okafor ◽  
Ochuko Erukainure ◽  
Atinuke Ajayi ◽  
Yewande Suberu ◽  
...  
Keyword(s):  
Protective Effect ◽  
Antioxidant Activities ◽  
Reduced Glutathione ◽  
Sperm Quality ◽  
Day Treatment ◽  
Quality Parameters ◽  
Diabetic Rats ◽  
Testicular Toxicity ◽  
Cervical Dislocation

Abstract: The protective effect of a developed drink from blends of selected fruits on the sperm quality of alloxan-induced diabetic rats was investigated.: Diabetes was intraperitoneally induced with alloxan; the formulated drink blend was orally administered 2.5 or 5 mL/day. Treatment lasted for 14 days and the rats were humanely sacrificed by cervical dislocation. The antioxidant status via assessment of reduced glutathione (GSH), catalase (CAT), superoxide dismutase (SOD) and lipid peroxidation (LPO) was carried out on the testis, while sperm cells were analyzed for sperm motility, counts and abnormality.: Induction of diabetes led to a significant (p<0.05) decrease in GSH level, elevated SOD and CAT activities, significant (p<0.05) decrease in the sperm quality parameters studied. However, treatment with the formulated drink led to a significant (p<0.05) reduction of LPO, SOD and CAT activities as well as increase in GSH level.: This study shows an improvement in testicular antioxidant activities and sperm qualities by single and double doses of the formulation, suggesting its protective potential against testicular toxicity in diabetic rats.

scholarly journals Effectiveness of Averrhoa bilimbi leaf extract on spermatogenic cells of mice (Mus Musculus L.) hyperglycemia

2021 ◽  
Vol 4 (2) ◽  
pp. 273-279
Author(s):  
Ida Ayu Manik Damayanti ◽  
Putu Indrayoni ◽  
Ni Wayan Sukma Antari ◽  
Anak Agung Istri Mas Padmiswari
Keyword(s):  
Leaf Extract ◽  
Sperm Quality ◽  
Quality Parameters ◽  
Control Group ◽  
P Value ◽  
Spermatogenic Cells ◽  
Male Mice ◽  
Control Group Design ◽  
Post Test

The purpose of this study was to determine the effect of giving Averrhoa bilimbi leaf extract on sperm quality of diabetic mice. This research is a pure experimental (true experimental) with a post-test-only control group design approach. This research was conducted by giving Averrhoa bilimbi leaf extract as a treatment for 42 days in male mice. Sperm quality parameters observed included viability, abnormalities, motility in sperm. In all variables, the results of the data showing a normal distribution with a p-value > 0.05 were then carried out with a parametric test using one-way ANOVA. Averrhoa bilimbi leaf extract can increase the number of spermatogenic cells in male mice with hyperglycemia.

scholarly journals Antioxidant Effects of Ocimum Gratissimum Leaf Essential Oils as a Supplement to Extender on Chilled Canine Sperm Quality

2020 ◽  
Author(s):  
Vui Van Nguyen ◽  
Samorn Ponchunchoovong ◽  
Sajeera Kupittayanant ◽  
Pakanit Kupittayanant
Keyword(s):  
Essential Oils ◽  
Sperm Quality ◽  
Antioxidant Properties ◽  
Quality Parameters ◽  
Control Group ◽  
Dependent Manner ◽  
Ocimum Gratissimum ◽  
Significant Difference ◽  
Antioxidant Effects

Abstract Background:Oxidative stress during chilled storage is a major problem withcanine sperm. To improve the quality of chilled canine sperm during storage, many synthetic antioxidants have been examined, but different outcomes were investigated depending on antioxidant properties. The bioactive compounds of essential oils fromOcimum gratissimumleaves are known as a natural antioxidant source. This study aimed to evaluate the antioxidant effects of essential oils from Ocimum gratissimumleavesas a supplement in extender on chilled canine sperm during 12 days of storage. Results:The results showed thatlow concentrations of Ocimum gratissimum essential oils (25, 50, and 100µg/mL) have beneficial effectson sperm quality, whereasOcimum gratissimumessential oils athigh levels (above 200µg/mL) have harmful effects. Specifically, the addition of 100µg/mL ofOcimum gratissimum essential oilsto the extender had the greatestbeneficial effect in improving the quality of chilled canine sperm, and had a significant difference in all sperm quality parameters except motility when compared to the control group (p<0.05). Conclusions:Ocimum gratissimum essential oilshave an impact on chilled canine sperm quality in a dose-dependent manner, and the best results areachieved with a maximum dose ofOcimum gratissimum essential oils of 100µg/mL.

Colloid single-layer centrifugation improves post-thaw donkey (Equus asinus) sperm quality and is related to ejaculate freezability

2015 ◽  
Vol 27 (2) ◽  
pp. 332 ◽  
Author(s):  
I. Ortiz ◽  
J. Dorado ◽  
D. Acha ◽  
M. J. Gálvez ◽  
M. Urbano ◽  
...  
Keyword(s):  
Sperm Quality ◽  
Single Layer ◽  
Quality Parameters ◽  
Sperm Parameters ◽  
Computer Assisted ◽  
Sperm Analysis ◽  
Equus Asinus ◽  
The Relationship ◽  
Fresh Semen

The aim of this study was to determine whether colloid single-layer centrifugation (SLC) improves post-thaw donkey sperm quality and if this potential enhancement is related to ejaculate freezability. Semen from Andalusian donkeys was frozen following a standard protocol. SLC was performed on frozen–thawed semen and post-thaw sperm parameters were compared with uncentrifuged samples. Sperm quality was estimated by integrating in a single value sperm motility (assessed by computer-assisted sperm analysis), morphology and viability (evaluated under brightfield or fluorescence microscopy). Sperm freezability was calculated as the relationship between sperm quality obtained before freezing and after thawing. Ejaculates were classified into low, medium and high freezability groups using the 25th and 75th percentiles as thresholds. All sperm parameters were significantly (P < 0.01) higher in SLC-selected samples in comparison to uncentrifuged frozen–thawed semen and several kinematic parameters were even higher than those obtained in fresh semen. The increment of sperm parameters after SLC selection was correlated with ejaculate freezability, obtaining the highest values after SLC in semen samples with low freezability. We concluded that, based on the sperm-quality parameters evaluated, SLC can be a suitable procedure to improve post-thaw sperm quality of cryopreserved donkey semen, in particular for those ejaculates with low freezability.

scholarly journals Influence of Ripening on Polyphenolic Content, Degradative, and Browning Enzymes in Cantaloupe Varieties (C. Melo, L.)

Horticulturae ◽  
2021 ◽  
Vol 7 (11) ◽  
pp. 421
Author(s):  
Filomena Monica Vella ◽  
Roberto Calandrelli ◽  
Bruna Laratta
Keyword(s):  
Cell Wall ◽  
Shelf Life ◽  
Consumer Preferences ◽  
Antioxidant Activities ◽  
Quality Parameters ◽  
Lytic Enzyme ◽  
Factors Affecting ◽  
Fresh Products ◽  
Cucumis Melo L

The biochemical changes that occur during the growth and ripening of fruit and vegetable tissues, especially for color and firmness, are the most important factors affecting the quality of fresh products. Cantaloupe (Cucumis melo, L.) is one of the main economically important fruits in the world and its quality parameters, e.g., sweetness, nutritional factors, and texture, influence consumer preferences. Hence, these two features, appearance and texture changes, were investigated in three different genotypes of netted melon, all characterized by an extended shelf life but with different ripening phases. In particular, in all melon cultivars, the cell wall-modifying enzymatic activities and indicators of softening as well as total polyphenols, ortho-diphenols, flavonoids, and tannins, and antioxidant activity were studied. One variety with excellent shelf-life displayed the best nutritional and healthy qualities, in the early stages of ripening, and the lowest degree of browning. The lytic enzyme activities were reduced in the initial stages and after they increased gradually until the overripe stage, with the same trend for all varieties under investigation. The antioxidant activities declined with increasing time of ripeness in all genotypes. The outcomes confirm that the activities of both classes examined, antioxidant and cell wall-modifying enzymes, may vary significantly during ripeness depending on the genotype, suggesting the involvement in determining the postharvest behavior of these fruits.

Hypoglycemic activity of Gleditsia caspica extract and its saponin-containing fraction in streptozotocin-induced diabetic rats

2016 ◽  
Vol 71 (7-8) ◽  
pp. 253-260 ◽  
Author(s):  
Sally A. El Awdan ◽  
Gehad A. Abdel Jaleel ◽  
Dalia O. Saleh ◽  
Marawan Abdel Basset ◽  
Farouk R. Melek ◽  
...  
Keyword(s):  
Methanolic Extract ◽  
Antioxidant Activities ◽  
Reduced Glutathione ◽  
Morphological Changes ◽  
Positive Control ◽  
Diabetic Rats ◽  
Albino Rats ◽  
Blood Samples ◽  
Single Intraperitoneal Injection ◽  
Fasted Rats

Abstract The fruits of Gleditsia species (Fabaceae) have been known in traditional medicine as a saponin-rich herbal medicine. The present study aimed to investigate the effects of the total methanolic extract of Gleditsia caspica (MEGC) and its saponin-containing fractions (SFGC) on hyperglycemia in streptozotocin (STZ)-induced diabetic rats. A single intraperitoneal injection of STZ (55 mg/kg body weight) was used to induce hyperglycemia in male albino rats. MEGC (15, 30 and 60 mg/kg, p.o.) and SFGC (15, 30 and 60 mg/kg, p.o.) were administered to the diabetic rats daily for 14 days. The anti-diabetic drug gliclazide (10 mg/kg, p.o.) was used as a positive control. Blood samples were collected from overnight fasted rats for the evaluation of the antihyperglycemic, antihyperlipidemic and antioxidant activities. The levels of glucose, triglycerides (TG), cholesterol (TC) and malondialdehyde (MDA) were increased significantly, whereas the levels of α-amylase, insulin and reduced glutathione (GSH) were decreased in the experimental diabetic rats. Pancreas and liver of the diabetic rats exhibited significant changes in the histopathology, morphology and DNA content. Administration of MEGC or SFGC led to a decrease in the levels of glucose, TG, TC and MDA. In addition, the levels of α-amylase, insulin and GSH were increased in MEGC and SFGC treated diabetic rats. Also, the histopathological and morphological changes, as well the changes in DNA were significantly reversed by the extracts. Thus, MEGC and SFGC exhibited potent hypoglycemic and hypolipidemic activities in STZ- induced diabetic rats.

scholarly journals Protective effects of peel extracts of Irvingia wombolu on metabolic disorders in streptozotocin-induced diabetic rats

2020 ◽  
Vol 6 (1) ◽  
Author(s):  
Rosane Soh Matsinkou ◽  
Valere Aime Soh Oumbe ◽  
Judith Laure Ngondi ◽  
Julius Enyong Oben
Keyword(s):  
Blood Glucose ◽  
Protective Effect ◽  
Total Cholesterol ◽  
Reduced Glutathione ◽  
Fasting Blood Glucose ◽  
Diabetic Rats ◽  
Protective Effects ◽  
Liver And Kidney ◽  
Heart Abnormalities ◽  
The Right

Abstract Background Diabetes is widely recognized as associated with several structural and functional liver, kidney, and heart abnormalities. Therefore, the present study was conducted to evaluate the protective effect of peel extracts of Irvingia wombolu against diabetes complications. Methods Diabetes was induced by intravenous administration of streptozotocin (STZ) (50 mg/kg) through the right jugular vein on rats and animals with blood glucose values of at least 250 mg/dl received orally aqueous extract of peel (AEP), hydroethanolic extract of peel (HEP), tolbutamide and DMSO 10%. Their effects on the concentration of blood glucose, total cholesterol, HDL-C, LDL-C, triglycerides, malondialdehyde (MDA) and activities of catalase (CAT), reduced glutathione (GSH), superoxide dismutase (SOD), aspartate aminotransferase (AST), and alanine aminotransferase (ALT) in diabetic and normal rats were investigated using standard methods. Results Twenty-one days of treatment with AEP and HEP at a dose of 400 mg/kg significantly (P < 0,05) reduced the fasting blood glucose to a point of reaching normal value. The antihyperlipidemic assessment of extracts revealed a significant (P < 0,05) decrease in total cholesterol, triglycerides, LDL levels, and a significant (P < 0,05) increase in HDL level in the plasma of treated diabetic rats. Furthermore, plasma biomarkers of liver and kidney dysfunction were significantly reduced in treated diabetic rats. We also observed increased activities of catalase, SOD, and reduced glutathione in diabetic treated rats. Conclusion The present findings suggest that AEP and HEP have a protective effect on liver, kidney, and heart in experimental diabetic rats which can be beneficial in the management of diabetes and its complications.

scholarly journals THE EFFECT OF CONSUMING AVOCADO (Persea americana) ON MICE (Mus musculus) SPERM QUALITY

2021 ◽  
Vol 7 (1) ◽  
pp. 11-17
Author(s):  
Yasinta Tiara Amelia ◽  
Evi Hanizar ◽  
Dwi Nur Rikhma Sari
Keyword(s):  
Sperm Morphology ◽  
Sperm Quality ◽  
Persea Americana ◽  
Sperm Number ◽  
Nacl Solution ◽  
Randomized Design ◽  
Smear Preparation ◽  
Cervical Dislocation ◽  
Completely Randomized Design

The nutrients such as protein and vitamin are proven to improve the sperm quality. One nutrient rich fruit is avocado round green variety, which contains vitamins A, C and E higher than other varieties. This study aimed to determine the effect of consuming avocado on the mice sperm quality. This experimental study was using a Completely Randomized Design with four treatments, each with eight replications. Avocado dosage treatments were control, 75 % w/v, 100% w/v, and 133% w/v , with the number of mice were 32 age four weeks. Avocado was given three times a day, each 0.5 mL for six weeks besides the main food. The observed response was the sperm quality including number, motility and morphology. Sperm was taken from the epididymis after the mice were killed by cervical dislocation method. Sperm was made a suspension using 0.9% NaCl solution and a smear preparation to observe the sperm morphology while staining with basic stain crystal violet. The sperm quality was observed by Neubauer's counting rooms through a multimedia microscope. Data was analyzed using Kurskal Wallis test for the sperm number and Anova test for motility and morphological responses. The result showed that avocado had a significant influence for reproductive health, particularly for increasing spermatozoa quality, including concentration, motility and morphology of sperm. The higher dose of avocado given the higher quantity and quality of sperm resulted.

Effect of boar ejaculate fraction, extender type and time of storage on quality of spermatozoa

2017 ◽  
Vol 20 (1) ◽  
pp. 77-84 ◽  
Author(s):  
A. Dziekońska ◽  
K. Świąder ◽  
M. Koziorowska-Gilun ◽  
K. Mietelska ◽  
Ł. Zasiadczyk ◽  
...  
Keyword(s):  
Sperm Quality ◽  
Membrane Integrity ◽  
Quality Parameters ◽  
Anova Analysis ◽  
Short Term ◽  
Straight Line ◽  
Boar Spermatozoa ◽  
Velocity Average

AbstractThe aim of this study was to investigate the effect the sperm-rich fraction (F1) and the post-F1 fraction (F2) on the quality of boar spermatozoa stored in a liquid state. Ejaculates were collected from three Polish Landrace boars. Each ejaculate fraction was diluted with BTS short-term extender and Safe-Cell Plus (SCP) long-term extender and stored for seven days (D1-D7) at 17°C. Analyses included sperm motility parameters, normal apical ridge (NAR) acrosomes and plasma membrane integrity (PMI). Prior to the dilution of fractions, marked changes (p<0.05) were noted between F1 and F2 in progressive motility (PMOT), velocity average pathway (VAP) and velocity straight line (VCL). After the ejaculate was diluted, the type of fraction and type of extender significantly affected (p<0.05) PMOT, being markedly higher (p<0.05) for F1 extended in BTS. No marked changes (p<0.05) were observed between F1 and F2 extended in SCP for any of the analyzed sperm quality parameters during seven days of storage. Significantly higher (p<0.05) values of sperm quality parameters were noted in F1 compared with F2 for BTS on D7 of storage. The results of the four-way ANOVA analysis indicate that boar, fraction of ejaculate, extender type and day of storage had significant effects on the quality of boar stored spermatozoa. The F1 was characterised by higher quality of spermatozoa during storage in comparison with F2 in the short-term extender. Using the long-term extender containing the proteins allowed for a better application of F2, which could be important for the pig industry.

Sign in / Sign up

Export Citation Format

Share Document