scholarly journals 5-Fluorouracil And Curcumin With Pectin Coating As a Treatment Regimen For Titanium Dioxide Induced Colon Cancer Model

2021 ◽  
Author(s):  
Chenmala Karthika ◽  
Raman Sureshkumar ◽  
Deepak Vasudevan Vasudevan Sajini ◽  
Gulam Md Ash ◽  
Md. Habibur Rahman
Keyword(s):  
Colorectal Cancer ◽  
Titanium Dioxide ◽  
Vero Cell ◽  
Cell Lines ◽  
Animal Studies ◽  
Cancer Model ◽  
Synergistic Approach ◽  
Hct 116 ◽  
Ft Ir ◽  
Vero Cell Lines

Abstract Objective: Induction of colorectal cancer in Wister rats using titanium dioxide and dimethylhydrazine and treatment using the physical conjugate of 5-Fluorouracil and Curcumin, with a synergistic approach.Methods: Compatibility studies are evaluated by using FT-IR,Vero cell lines, and HCT-116 cell lines are used for evaluating the synergistic approach. This followed by induction using titanium dioxide and dimethylhydrazine in Wister rats and treatment using 5-Fluorouracil and Curcumin with pectin coating. Result: The samples were found to be compatible. The synergistic effect was obtained at 1:1, 1:2, 1:4, and 2:1 ratio, where 1:4 ratio shows a CI50 value of 0.896, selected further for the animal studies when studied in HCT 116 cell lines and found to be safe with Vero cell lines. Colorectal cancer was shown to be induced within 70 days of administration of titanium dioxide and dimethylhydrazine.1:4 ratio of 5-Fluorouracil and Curcumin (50:200) shows effective for the treatment of colorectal cancer within 28 days, proven using histopathology report, bodyweight analysis, and hematological reports.Conclusion: 5-Fluorouracil and curcumin (1:4) ratio works with a synergistic approach and was proven effective for the treatment of colorectal cancer induced bythe titanium dioxide and dimethylhydrazine.

scholarly journals Whole-Genome Sequences of Zika Virus FLR Strains after Passage in Vero or C6/36 Cells

2018 ◽  
Vol 6 (4) ◽  
Author(s):  
Lindsey A. Moser ◽  
Lauren M. Oldfield ◽  
Nadia Fedorova ◽  
Vinita Puri ◽  
Susmita Shrivastava ◽  
...  
Keyword(s):  
Vero Cell ◽  
Cell Lines ◽  
Virus Strain ◽  
Zika Virus ◽  
Whole Genome ◽  
Genome Sequences ◽  
Complete Genomes ◽  
Vero Cell Lines

ABSTRACT We report 26 complete genomes of Zika virus (ZIKV) isolated after passaging the Zika virus strain FLR in mosquito (C6/36) and mammalian (Vero) cell lines. The consensus ZIKV genomes we recovered show greater than 99% nucleotide identify with each other and with the FLR strain used as input.

Effect of Different Hydatid Cyst Molecules on Hela and Vero Cell Lines Growth In vitro

2013 ◽  
Vol 02 (01) ◽  
Author(s):  
Nasir Aref ◽  
Hedayatollah Shirzad ◽  
Morteza Yousefi ◽  
Hossein Yousofi.Darani
Keyword(s):  
Hydatid Cyst ◽  
Vero Cell ◽  
Cell Lines ◽  
Vero Cell Lines

scholarly journals Growth and Maintenance of Vero Cell Lines

2008 ◽  
Vol 11 (1) ◽  
Author(s):  
Nicole C. Ammerman ◽  
Magda Beier‐Sexton ◽  
Abdu F. Azad
Keyword(s):  
Vero Cell ◽  
Cell Lines ◽  
Vero Cell Lines

scholarly journals Autophagic Cell Death Is Induced by Acetone and Ethyl Acetate Extracts fromEupatorium odoratum In Vitro: Effects on MCF-7 and Vero Cell Lines

2012 ◽  
Vol 2012 ◽  
pp. 1-9 ◽  
Author(s):  
Faizah Bt. Harun ◽  
Syed Mohsin Syed Sahil Jamalullail ◽  
Khoo Boon Yin ◽  
Zulkhairi Othman ◽  
Anita Tilwari ◽  
...  
Keyword(s):  
Cell Death ◽  
Ethyl Acetate ◽  
Cytotoxic Activity ◽  
Vero Cell ◽  
Cell Lines ◽  
Autophagic Cell Death ◽  
Biologically Active ◽  
Vero Cell Lines ◽  
Mcf 7 ◽  
Ethyl Acetate Extracts

Eupatorium odoratum (EO)contains many biologically active compounds, the anticancer effects of which are not well documented. This study evaluates the cytotoxic effects and mechanism of action ofEOextracts on MCF-7 and Vero cell lines. Evaluation of the cytotoxic activity using MTT assay, morphological alterations, and apoptosis were carried out. Autophagy was evaluated by LC3-A protein expression. Cytotoxic activity, membrane blebbing and ballooning at 24 hours, replacement by mass vacuolation, and double membrane vesicles mimicking autophagy and cell death were observed in the cancer cells. No apoptosis was observed by DNA fragmentation assay. Overexpression of LC3-A protein indicated autophagic cell death. Cell cycle analysis showed G0 and G2/M arrest. The Vero cells did not show significant cell death at concentrations <100 μg/mL. These results thus suggest that acetone and ethyl acetate extracts ofEOinduce cell death through induction of autophagy and hold potential for development as potential anticancer drugs.

scholarly journals In vitro anticancer property of Solanum mammosum callus culture against HeLa and Vero cell lines

2020 ◽  
Vol 24 (2) ◽  
pp. 218-224
Author(s):  
SUCIATI SUCIATI ◽  
Lusiana ARIFIANTI ◽  
Andiena ELSAFIRA ◽  
Lovely Q. ILMIAH
Keyword(s):  
Vero Cell ◽  
Cell Lines ◽  
Callus Culture ◽  
Solanum Mammosum ◽  
Vero Cell Lines
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