scholarly journals Multi-omics Analyses Revealed Key Factors Involved in Fluorescent Carbon-Dots-Regulated Secondary Metabolism in Tetrastigma Hemsleyanum

Author(s):  
Xin Peng ◽  
Zhuomi Xie ◽  
Xiuhua Wang ◽  
Yuxiang Zhao ◽  
Chuyun Yang ◽  
...  

Abstract Background: Luminescent nanomaterials (LNMs), especially newly-exploited fluorescent carbon-dots (CDs), have demonstrated promising candidates for sunlight harvesting and enhanced photosynthesis efficiency of crops. However, most of the studies focus on the design and synthesis of LNMs and primary metabolism in biomass acceleration, secondary metabolism that closely associated with the quality ingredients of plants is rarely mentioned. Results: UV-absorptive and water-soluble NIR-CDs were harvested via a facile microwave-assisted carbonization method. The effect and regulatory mechanism of NIR-CDs on the secondary metabolism and bioactive ingredients accumulation in Tetrastigma hemsleyanum were explored. A total of 191 differential secondary metabolites and 6874 differentially expressed genes were identified when the NIR-CDs were adopted for enhancing growth of T. hemsleyanum. The phenolic acids were generally improved, but the flavonoids were more likely to decrease. The pivotal differentially expressed genes were involved in biosynthesis of secondary metabolites, flavonoid biosynthesis, porphyrin and chlorophyll metabolism, etc. The gene-metabolite association was constructed and 44 hub genes highly related to quality ingredients accumulation and growth were identified, among which and the top 5 genes of the PPI network might be the key regulators.Conclusion: This research provided key regulatory genes and differentially accumulating quality ingredients under NIR-CDs-treatment, which could provide a theoretical basis for expanding the applications of nanomaterial in industrial crop agriculture.

Molecules ◽  
2021 ◽  
Vol 26 (5) ◽  
pp. 1246
Author(s):  
Tengfei Wang ◽  
Hui Luo ◽  
Xu Jing ◽  
Jiali Yang ◽  
Meijun Huo ◽  
...  

Water-soluble fluorescent carbon dots (CDs) were synthesized by a hydrothermal method using citric acid as the carbon source and ethylenediamine as the nitrogen source. The repeated and scale-up synthetic experiments were carried out to explore the feasibility of macroscopic preparation of CDs. The CDs/Fe3+ composite was prepared by the interaction of the CDs solution and Fe3+ solution. The optical properties, pH dependence and stability behavior of CDs or the CDs/Fe3+ composite were studied by ultraviolet spectroscopy and fluorescence spectroscopy. Following the principles of fluorescence quenching after the addition of Fe3+ and then the fluorescence recovery after the addition of asorbic acid, the fluorescence intensity of the carbon dots was measured at λex = 360 nm, λem = 460 nm. The content of ascorbic acid was calculated by quantitative analysis of the changing fluorescence intensity. The CDs/Fe3+ composite was applied to the determination of different active molecules, and it was found that the composite had specific recognition of ascorbic acid and showed an excellent linear relationship in 5.0–350.0 μmol·L−1. Moreover, the detection limit was 3.11 μmol·L−1. Satisfactory results were achieved when the method was applied to the ascorbic acid determination in jujube fruit. The fluorescent carbon dots composites prepared in this study may have broad application prospects in a rapid, sensitive and trace determination of ascorbic acid content during food processing.


2016 ◽  
Vol 8 (48) ◽  
pp. 8554-8565 ◽  
Author(s):  
Jie Wang ◽  
Fengxian Qiu ◽  
Haiyan Wu ◽  
Xin Li ◽  
Tao Zhang ◽  
...  

A novel water-soluble chitosan linked fluorescent carbon dots and isophorone diisocyanate toward detection of chromium(vi).


RSC Advances ◽  
2017 ◽  
Vol 7 (53) ◽  
pp. 33459-33465 ◽  
Author(s):  
Yinjian Zheng ◽  
Haoran Zhang ◽  
Wei Li ◽  
Yingliang Liu ◽  
Xuejie Zhang ◽  
...  

Carbon dots (CDs), synthesized from biological sources, have attracted attention in bioimaging and bioscience due to their low cytotoxicity, water-soluble nature, and biocompatibility.


2016 ◽  
Vol 18 (1) ◽  
pp. 243-250 ◽  
Author(s):  
Jeremy B. Essner ◽  
Charles H. Laber ◽  
Sudhir Ravula ◽  
Luis Polo-Parada ◽  
Gary A. Baker

We have demonstrated an easy, economic, one-step synthetic route to water-soluble fluorescent carbon dots derived from the thermal upcycling of urine.


2018 ◽  
Vol 10 (1) ◽  
pp. 151-157 ◽  
Author(s):  
Haoying Zhai ◽  
Baozhan Zheng ◽  
Feng Yang ◽  
Meina Wang ◽  
Dan Xiao

Fluorescent carbon dots (CDs) were successfully synthesized via pyrolysis of Setcreasea purpurea boom, providing a simple and convenient method for the preparation of CDs without using toxic or expensive materials.


2020 ◽  
pp. 174751982095382
Author(s):  
Hasan Eskalen ◽  
Mustafa Çeşme ◽  
Süleyman Kerli ◽  
Şükrü Özğan

Blue fluorescent carbon dots are synthesized via a one-step hydrothermal method using rosemary leaves as a carbon source. The obtained carbon dots are characterized by X-ray diffraction, transmission electron microscopy, X-ray photoelectron spectroscopy, UV-Vis, FTIR, and fluorescence spectrometry. The results show that carbon dots gave a maximum emission peak at 422 nm when excited at 332 nm. The average particle diameter of the synthesized spherical carbon dots is found to be 16.13 nm with a 4.6-nm standard deviation. The diameters of the produced carbon dots vary, as is evident from standard deviation heights, resulting in multicolor emission spectra. The fruit storage experiment is carried out by investigating the qualitative antioxidant ability of the produced carbon dots. The results obtained for polyvinyl alcohol–carbon dot–coated samples differ remarkably compared to polyvinyl alcohol–coated samples only. The polyvinyl alcohol–carbon dot composition is used in latent fingerprint detection applications. In analyses made on a glass surface, fingerprints cannot be monitored on the glass surface with the polyvinyl alcohol layer under ultraviolet light, while they are observed on the surface covered with the polyvinyl alcohol–carbon dot composition. Moreover, biological activity against Gram-positive, Gram-negative, and yeast strains are investigated.


2014 ◽  
Vol 38 (3) ◽  
pp. 906 ◽  
Author(s):  
Jumeng Wei ◽  
Xin Zhang ◽  
Yingzhuo Sheng ◽  
Jianmin Shen ◽  
Peng Huang ◽  
...  

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