Potential Anticarcinogenic Effects From Plasma of Elderly After Exercise Training: A Pilot Study

Abstract This study aimed to evaluate the impact of exercise training plasma on in vitro prostate cancer cell viability and proliferation. PC3 prostate cancer cells were incubated with plasma obtained from young women with high and low physical fitness (PF) and with the plasma collected from institutionalized elderly before and after multimodal exercise training. Plasma from High PF women induced the lowest cell viability and proliferation after incubation time. PC3 cells presented lower cell viability and diminished rates of cell proliferation after the incubation with post-training plasma samples of elderly. The incubation of PC3 cells with post-training plasma of elderly decreased the mitochondrial membrane polarization and increased mitochondrial reactive oxygen species (ROS) production without changes in cytosolic ROS. Post-training plasma did not change apoptosis or necrosis rates in the PC-3 cell line. Multimodal exercise training increased the plasma levels of IL-2, IL-10, IFN-α, and FGF-1, and decreased TNF-α concentrations in institutionalized elderly. In conclusion, we showed that systemic adaptations in plasma mediators of institutionalized elderly may alter cell viability and proliferation by targeting mitochondrial ROS in a prostate cancer cell line.

Download Full-text

Downregulation of HMGA2 by Small Interfering RNA Affects the Survival, Migration, and Apoptosis of Prostate Cancer Cell Line

Advanced Pharmaceutical Bulletin ◽

10.34172/apb.2022.039 ◽

2021 ◽

Author(s):

Shima Khajouee ◽

Elham Baghbani ◽

Ali Mohammadi ◽

Behzad Mansoori ◽

Dariush Shanehbandi ◽

...

Keyword(s):

Prostate Cancer ◽

Cell Line ◽

Cancer Cell ◽

Cancer Progression ◽

Prostate Cancer Cell ◽

Cancer Cell Line ◽

Prostate Cancer Cell Line ◽

Dependent Manner ◽

Pc3 Cells ◽

The Impact

Purpose: To investigate the downregulation of High Mobility Group AT-hook 2 (HMGA2) expression by small interfering RNAs (siRNAs) in PC3 prostate cancer cell line. HMGA2 belongs to the non-histone chromatin-binding protein family that serves as a crucial regulator of gene transcription. The overexpression of this gene is positively correlated with various prostate cancer-related properties. Thus, HMGA2 is an emerging target in prostate cancer treatment. This study aimed to examine the impact of siRNAs targeting HMGA2 on the viability, migration, and apoptosis processes of the PC3 prostate cancer cell line. Methods: siRNA transfection was conducted with a liposome-mediated approach. The mRNA and protein expression levels for HMGA2 are evaluated by qRT-PCR and western blot analysis. The cytotoxic properties of HMGA2-siRNA were measured by MTT assay on PC3 cells. The migration of PC3 cells was measured by implementing a wound-healing assay. Apoptosis measurement was also quantified by TUNEL assay. Results: Transfection with siRNA significantly decreased both mRNA and protein levels of the HMGA2 gene in a dose-dependent manner after 48 hours. Also, we demonstrated that the knockdown of HMGA2 led to a reduction in cell viability, migration ability, and enhanced apoptosis of PC3 cells in vitro. Conclusion: Our findings recommend that the specific siRNA of HMGA2 may efficiently be able to decrease prostate cancer progression. Therefore, it may be a promising adjuvant treatment in prostate cancer.

Download Full-text

Anticarcinogenic effects from plasma elderly trained: the role of mitochondrial function

10.1101/2021.09.08.21263280 ◽

2021 ◽

Author(s):

Alessandra Peres ◽

Gilson Pires Dorneles ◽

Gisele Branchini ◽

Fernanda Bordignon Nunes ◽

Pedro RT Romão ◽

...

Keyword(s):

Prostate Cancer ◽

Growth Factor ◽

Exercise Training ◽

Cell Viability ◽

Cell Line ◽

Cancer Cell ◽

Prostate Cancer Cell ◽

Cancer Cell Line ◽

Prostate Cancer Cell Line ◽

Before And After

This study aimed to evaluate the effects of multimodal exercise training on systemic cytokine levels of the elderly, and the impact of post-exercise training plasma on prostate cancer cell viability and proliferation in vitro. Fasting blood samples were collected from eight institutionalized elderly before and after eight weeks of multimodal exercise training (twice a week). The levels of interleukin(IL)-1ra, IL-1β, IL-2, IL-6, IL-10, IL-17, interferon (IFN)-α, tumor necrosis factor (TNF)-α, fibroblast growth factor (FGF)-1, platelet-derived growth factor (PDGF) and transforming growth factor (TGF)-α were evaluated in the peripheral blood. PC3 prostate cancer cell line was incubated with 10% plasma acquired before and after exercise training from each participant. Multimodal exercise training increased the plasma levels of IL-2, IL-10, IFN-α, and FGF-1, and decreased TNF-α concentrations. PC3 cells presented decreased cell viability evaluated by MTT and lactate dehydrogenase activity as well as lower rates of cell proliferation after the incubation with post-training plasma samples. The incubation of PC-3 cells with post-training plasma decreased the mitochondrial membrane polarization and increased mitochondrial reactive oxygen species (ROS) production without changes in cytosolic ROS. Post-training plasma did not change apoptosis or necrosis rates in the PC-3 cell line. In conclusion, we showed that systemic adaptations in plasma mediators of institutionalized elderly might alter cell viability and proliferation by targeting mitochondrial ROS in a prostate cancer cell line.

Download Full-text

PENGARUH EKSTRAK METANOL DAUN SIRSAK (Annona muricata Linn.) TERHADAP VIABILITAS GALUR SEL KANKER PROSTAT

Majalah Kedokteran Andalas ◽

10.22338/mka.v37.i3.p187-197.2014 ◽

2015 ◽

Vol 37 (3) ◽

pp. 187

Author(s):

Retno Yulianti ◽

Ria Kodariah ◽

Puspita Ekawuyung

Keyword(s):

Prostate Cancer ◽

Cell Viability ◽

Cell Line ◽

Cancer Cell ◽

Cell Morphology ◽

Methanol Extract ◽

Prostate Cancer Cell ◽

Control Group ◽

Annonaceous Acetogenins

AbstrakDaun sirsak mengandung senyawa aktif annonaceous acetogenins yang memiliki efek sitotoksik pada sel kanker. Penelitian ini bertujuan untuk mengetahui pengaruh ekstrak daun sirsak terhadap viabilitas dan peningkatan daya hambat terhadap galur sel kanker prostat PC3. Desain penelitian adalah eksperimental in vitro. Subyek penelitian adalah cell line PC3 yang terbagi 5 kelompok yaitu kelompok kontrol sel, kelompok perlakuan dengan ekstrak metanol daun sirsak (EMDS) dengan konsentrasi 6,25; 12,5 dan 25 mg/mL dan kelompok doksorubisin. Kelompok perlakuan diuji viabilitas sel dengan MTT assay pada inkubasi 0 dan 24 jam dan dilakukan pengamatan morfologi sel. Data dianalisis dengan uji statistik ANOVA. Hasil penelitian menunjukkan penurunan nilai OD pada kelompok EMDS 6,25 dan 12,5 ug/mL, namun uji statistik tidak berbeda bermakna dan kemampuan menghambat viabilitas sel paling besar ada pada kelompok EMDS 12,5 ug/mL (nilai OD 0,94). Pengamatan morfologi sel menunjukkan efek sitotoksik. Kesimpulan: Ekstrak metanol daun sirsak memiliki peran potensial sebagai antikanker terhadap galur sel kanker prostat PC3 meskipun sangat kecil efek penghambatannya.AbstractSoursop leaves contain annonaceous acetogenins active compounds that have a cytotoxic effect on cancer cells. The aim of this study is to determine the effect of soursop leaf extract on the viability and inhibitory rate on the prostate cancer cell line PC3. The study was an experimental in vitro study. Subjects were 5 groups of PC3 cell line: cell control group, the group treated with methanol extract of soursop leaves (EMDS) with the concentrations of 6.25; 12.5 and 25 mg/mL and the doxorubicin group. The groups were tested using the MTT cell viability assay at 0 and 24 hours of incubation followed by PC3 cell morphology examination. Data were analyzed by ANOVA test. The results showed a decrease in the OD value of 6.25 and 12.5 ug/mL EMDS group, but statistical tests did not differ significantly and the EMDS 12.5 mg/mL group showed the highest ability in inhibiting cell viability (OD 0.94). Observation of cell morphology showed cytotoxic effects. Conclusion: The methanol extract of soursop leaf has a potential as an anticancer against prostate cancer cell lines despite the very small inhibitory effect.

Download Full-text