scholarly journals Ex vivo Fluorescence Confocal Microscopy: Chances and Changes in the Analysis of Breast Tissue

2021 ◽  
Author(s):  
Maja Carina Nackenhorst ◽  
Mohammad Kasiri ◽  
Bernd Gollackner ◽  
Heinz Regele
Keyword(s):  
Adipose Tissue ◽  
Confocal Microscopy ◽  
Ex Vivo ◽  
Tissue Imaging ◽  
Tissue Analysis ◽  
Frozen Sections ◽  
Histologic Diagnosis ◽  
Tissue Samples ◽  
Fluorescence Confocal Microscopy ◽  
Medical University

Abstract Background: Rapid histologic diagnosis of frozen sections is essential for a variety of surgical procedures. Frozen sections however, require specialized lab equipment, are prone to freezing artifacts and are not applicable to all types of tissue. Adipose tissue is especially difficult to process in frozen sections. Although these limitations are well known, no alternative method for microscopic tissue analysis that might replace frozen sections could be established. Our objective was to evaluate whether tissue imaging based on ex vivo fluorescent confocal microscopy (FCM) is applicable for rapid microscopic assessment of breast tumors specimens with abundant adipose tissue.Methods: We evaluated 17 tissue samples from mastectomy specimens, rich in adipose tissue, submitted to the department of pathology at the Medical University of Vienna. We conducted our study on the FCM VivaScope® 2500M-G4 (Mavig GmbH, Munich, Germany; Caliber I.D.; Rochester NY, USA).Results: When comparing FCM to frozen sections, we found a very similar overall processing time for FCM images and frozen sections respectively. Image quality was mostly superior to frozen sections (especially for adipose tissue and nuclear detail) but inferior to H&E stained FFPE sections. Limitations of the technology were uneven coloring, invisibility of ink applied for marking tissue margins and distortion artifacts if too much pressure is applied to the tissue. Conclusion: FCM has the potential to expand the application and usefulness of rapid tissue analysis as speed is comparable and quality exceeds that of frozen sections especially in tissues rich in adipose cells such as breast specimen.

scholarly journals Features of oral squamous cell carcinoma in ex vivo fluorescence confocal microscopy

2020 ◽  
Author(s):  
Veronika Shavlokhova ◽  
Christa Flechtenmacher ◽  
Sameena Sandhu ◽  
Michael Vollmer ◽  
Jürgen Hoffmann ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Confocal Microscopy ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Ex Vivo ◽  
Fluorescence Confocal Microscopy

scholarly journals Vitamin D alleviates oxidative stress in adipose tissue and mesenteric vessels from obese patients with subclinical inflammation

2020 ◽  
Vol 98 (2) ◽  
pp. 85-92 ◽  
Author(s):  
Mihaela Ionica ◽  
Oana M. Aburel ◽  
Adrian Vaduva ◽  
Alexandra Petrus ◽  
Sonia Rațiu ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Vitamin D ◽  
Adipose Tissue ◽  
Vascular Function ◽  
Ex Vivo ◽  
Active Form ◽  
Reactive Oxygen Species Generation ◽  
Obese Patients ◽  
Tissue Samples

Obesity is an age-independent, lifestyle-triggered, pandemic disease associated with both endothelial and visceral adipose tissue (VAT) dysfunction leading to cardiometabolic complications mediated via increased oxidative stress and persistent chronic inflammation. The purpose of the present study was to assess the oxidative stress in VAT and vascular samples and the effect of in vitro administration of vitamin D. VAT and mesenteric artery branches were harvested during abdominal surgery performed on patients referred for general surgery (n = 30) that were randomized into two subgroups: nonobese and obese. Serum levels of C-reactive protein (CRP) and vitamin D were measured. Tissue samples were treated or not with the active form of vitamin D: 1,25(OH)2D3 (100 nmol/L, 12 h). The main findings are that in obese patients, (i) a low vitamin D status was associated with increased inflammatory markers and reactive oxygen species generation in VAT and vascular samples and (ii) in vitro incubation with vitamin D alleviated oxidative stress in VAT and vascular preparations and also improved the vascular function. We report here that the serum level of vitamin D is inversely correlated with the magnitude of oxidative stress in the adipose tissue. Ex vivo treatment with active vitamin D mitigated obesity-related oxidative stress.

Evaluating ex vivo fluorescence confocal microscopy images of basal cell carcinomas in M ohs excised tissue

2014 ◽  
Vol 171 (3) ◽  
pp. 561-570 ◽  
Author(s):  
C. Longo ◽  
M. Rajadhyaksha ◽  
M. Ragazzi ◽  
K. Nehal ◽  
S. Gardini ◽  
...  
Keyword(s):  
Confocal Microscopy ◽  
Basal Cell ◽  
Ex Vivo ◽  
Fluorescence Confocal Microscopy ◽  
Basal Cell Carcinomas ◽  
Excised Tissue ◽  
Microscopy Images

scholarly journals Prospective evaluation of the utility of intraoperative confocal laser endomicroscopy in patients with brain neoplasms using fluorescein sodium: experience with 74 cases

2016 ◽  
Vol 40 (3) ◽  
pp. E11 ◽  
Author(s):  
Nikolay L. Martirosyan ◽  
Jennifer M. Eschbacher ◽  
M. Yashar S. Kalani ◽  
Jay D. Turner ◽  
Evgenii Belykh ◽  
...  
Keyword(s):  
Brain Tumors ◽  
Ex Vivo ◽  
Confocal Laser Endomicroscopy ◽  
Confocal Laser ◽  
Fluorescein Sodium ◽  
Frozen Sections ◽  
Diagnostic Image ◽  
Tissue Samples ◽  
Specificity And Sensitivity

OBJECTIVE This study evaluated the utility, specificity, and sensitivity of intraoperative confocal laser endomicroscopy (CLE) to provide diagnostic information during resection of human brain tumors. METHODS CLE imaging was used in the resection of intracranial neoplasms in 74 consecutive patients (31 male; mean age 47.5 years; sequential 10-month study period). Intraoperative in vivo and ex vivo CLE was performed after intravenous injection of fluorescein sodium (FNa). Tissue samples from CLE imaging–matched areas were acquired for comparison with routine histological analysis (frozen and permanent sections). CLE images were classified as diagnostic or nondiagnostic. The specificities and sensitivities of CLE and frozen sections for gliomas and meningiomas were calculated using permanent histological sections as the standard. RESULTS CLE images were obtained for each patient. The mean duration of intraoperative CLE system use was 15.7 minutes (range 3–73 minutes). A total of 20,734 CLE images were correlated with 267 biopsy specimens (mean number of images/biopsy location, in vivo 84, ex vivo 70). CLE images were diagnostic for 45.98% in vivo and 52.97% ex vivo specimens. After initiation of CLE, an average of 14 in vivo images and 7 ex vivo images were acquired before identification of a first diagnostic image. CLE specificity and sensitivity were, respectively, 94% and 91% for gliomas and 93% and 97% for meningiomas. CONCLUSIONS CLE with FNa provided intraoperative histological information during brain tumor removal. Specificities and sensitivities of CLE for gliomas and meningiomas were comparable to those for frozen sections. These data suggest that CLE could allow the interactive identification of tumor areas, substantially improving intraoperative decisions during the resection of brain tumors.

scholarly journals Ex vivo fluorescence confocal microscopy: the first application for real-time pathological examination of prostatic tissue

2019 ◽  
Vol 124 (3) ◽  
pp. 469-476 ◽  
Author(s):  
Stefano Puliatti ◽  
Laura Bertoni ◽  
Giacomo M. Pirola ◽  
Paola Azzoni ◽  
Luigi Bevilacqua ◽  
...  
Keyword(s):  
Confocal Microscopy ◽  
Real Time ◽  
Ex Vivo ◽  
Prostatic Tissue ◽  
Pathological Examination ◽  
Fluorescence Confocal Microscopy
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