scholarly journals Genetic Characterization and Recombinant History of a Novel Emerging HIV-1 Second-Generation Circulating Recombinant Form (CRF120_0107) Identified Among Sexually Transmitted Infections in Shenzhen, China

2021 ◽  
Author(s):  
Bo Zhu ◽  
Jin Zhao ◽  
Xiaorui Wang ◽  
Hanping Li ◽  
Yongjian Liu ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Second Generation ◽  
Dual Infection ◽  
Sexually Transmitted ◽  
History Of ◽  
High Bootstrap ◽  
Sex With Men ◽  
Full Length Genome ◽  
Monophyletic Branch ◽  
Hiv 1

Abstract Under the background of the main epidemic HIV strains (CRF01_AE and CRF07_BC) co-circulation in China, more HIV second-generation recombinant (SGR) strains with CRF01_AE and CRF07_BC as the backbone are emerging. In this study, we analyzed the characteristics and evolutionary history of a newly emerging HIV-1 CRF120_0107 composed of CRF01_AE and CRF07_BC based on the near full-length genome (NFLG) in Shenzhen, Guangdong Province, China. NFLG phylogenetic analysis revealed that these sequences formed a distinct monophyletic branch with a high bootstrap value (>90%), distantly related to all known HIV-1 genotypes. Recombination analysis showed that CRF120_0107 was composed of the predominant HIV-1 strains in China: CRF01_AE and CRF07_BC. Further subregional phylogenetic analysis was performed that possible parental lineages of CRF07_BC segments (Ⅰ, Ⅲ, and Ⅴ) belonged to the CRF07_BC men who have sex with men cluster (MSM cluster), other CRF01_AE segments also mainly belonged to MSM Cluster (such as CRF01_AE Cluster 5). Bayesian analysis results inferred that CRF120_0107 placed its emergence in Shenzhen approximately between 2009-2011. The appearance of CRF120_0107 further highlights that more and more HIV-1 SGR strains containing CRF01_AE and CRF07_BC will be more generated frequently and will most likely be more conducive to accelerating the spread of HIV in China. This highlighted it is necessary to monitor MSM high-risk individuals with HIV-1 CRF01_AE and CRF07_BC dual infection to prevent the generation of CRF01_AE/CRF07_BC recombinant strains, thus reducing the possibility of HIV-1 genotype resistance and the complexity of treatment in China.

The identification of a novel HIV-1 second-generation recombinant form (CRF01_AE/CRF07_BC) among men who have sex with men in Jiangsu, China

2020 ◽  
Vol 18 ◽  
Author(s):  
Yin Yueqi ◽  
Zhou Ying ◽  
Lu Jing ◽  
Guo Hongxiong ◽  
Chen Jianshuang ◽  
...  
Keyword(s):  
Maximum Likelihood ◽  
Phylogenetic Trees ◽  
Second Generation ◽  
Emergency Situation ◽  
The World ◽  
Break Points ◽  
Sex With Men ◽  
Full Length Genome ◽  
Virus Strains ◽  
Hiv 1

Background: CRF01_AE and CRF07_BC are the two major HIV-1 virus strains circulating in China. The proportion of dominant subtypes (CRF01_AE and CRF07_BC) among MSM in Jiangsu province was over 80%. A large number of URFs have been found in China in recently years. Objective: This study aimed to report on novel HIV-1 recombinants. Method: We constructed Phylogenetic trees using the maximum likelihood (ML) method with 1000 bootstrap replicates in IQ-TREE 1.6.8 software and determined recombination break points using SimPlot 3.5.1. Results: We identified a novel, second-generation HIV-1 recombinant (JS020202) between CRF01_AE and CRF07_BC. The analysis of near full-length genome (NFLG) showed there were at least 8 breakpoints inner virus, which differed from any previously identified CRF and URF around the world. Conclusion: Novel diverse CRF01_AE/07_BC suggested the complexity trends of HIV-1 genetics. The emergency situation of diverse recombinant strains should be monitored continuously.

scholarly journals First Detection of a Cluster Novel HIV-1 Second-Generation Recombinant (CRF01_AE/CRF07_BC) among Men Who Have Sex with Men in Nanjing, Eastern China

Intervirology ◽  
2021 ◽  
Vol 64 (2) ◽  
pp. 81-87 ◽  
Author(s):  
Yueqi Yin ◽  
Ying Zhou ◽  
Jing Lu ◽  
Hongxiong Guo ◽  
Jianshuang Chen ◽  
...  
Keyword(s):  
Phylogenetic Trees ◽  
Second Generation ◽  
Eastern China ◽  
Northern China ◽  
Small Scale ◽  
Transmission Networks ◽  
Nanjing City ◽  
Sex With Men ◽  
Full Length Genome ◽  
Hiv 1

<b><i>Introduction:</i></b> A large number of unique recombinant forms have been found in China in recent years. This study aimed to report on a cluster of novel HIV-1 recombinants. <b><i>Methods:</i></b> We constructed phylogenetic trees using the maximum likelihood (ML) method with 1,000 bootstrap replicates in IQ-TREE 1.6.8 software and determined recombination break points using SimPlot 3.5.1. <b><i>Results:</i></b> Overall, 9 near-full-length genome (NFLG) sequences were reported in this study, including 1 circulation recombinant form (CRF)01_AE NFLG sequence and 8 highly similar novel HIV-1 second-generation recombinants composed of CRF01_AE and CRF07_BC (CRF105_0107) isolated from a cluster HIV-positive male subjects infected among men who have sex with men (MSM) in Nanjing, eastern China. The phylogenetic analysis of NFLG showed 1 sequence named “nj16” to have at least 11 breakpoints inner virus and 7 other sequences to have at least 10 breakpoints inner virus. Our findings further showed as follows: first, this is the first time that a cluster of novel CRF105_0107 HIV-1 strains were identified among MSM in Nanjing, Jiangsu. Second, the Chinese “4a” cluster of CRF01_AE which mainly circulating in northern China has spread in Jiangsu for more than 15 years. Third, HIV-1 recombination events were active in Nanjing city, and novel recombinants could spread rapidly through some small-scale transmission networks. <b><i>Conclusion:</i></b> The continued emergence of novel recombinant HIV-1 strains in Nanjing suggests dynamics and complexity in the HIV epidemic among MSM in Jiangsu province. Further investigations and molecular epidemiological research should be taken to monitor and understand transmission networks among MSM.

scholarly journals Identification of Two Similar Novel HIV-1 Recombinant Forms (CRF01_AE/CRF07_BC) among Intravenous Drug Users in Guangxi, China

Intervirology ◽  
2021 ◽  
pp. 1-9
Author(s):  
Fei Zhang ◽  
Yao Yang ◽  
Bingyu Liang ◽  
Yuan Yang ◽  
Qiuyu Wei ◽  
...  
Keyword(s):  
Drug Users ◽  
Intravenous Drug ◽  
Epidemiological Surveillance ◽  
Intravenous Drug Users ◽  
High Bootstrap ◽  
Full Length Genome ◽  
New Evidence ◽  
Monophyletic Branch ◽  
Cluster 2 ◽  
Hiv 1

New kinds of HIV-1 circulating recombinant forms (CRFs) and unique recombinant forms (URFs) earn a great prevalence in China nowadays. In this study, we identified 2 similar URFs (2016GXNNIDU037 and 2019QZLSIDU253) both isolated from intravenous drug users (IDUs) in Guangxi, China. Phylogenetic analysis of the near full-length genome (NFLG) revealed 2 URFs both clustered with CRF01_AE but setting up a monophyletic branch, supporting a high bootstrap value. Bootscan analysis and subregional recombinant analysis found that the NFLG of 2016GXNNIDU037 and 2019QZLSIDU253 were both composed of CRF01_AE and CRF07_BC, with 3 CRF07_BC mosaic segments inserted into CRF01_AE backbones. The CRF01_AE segments of the 2 URFs clustered with a previously reported cluster 2 lineage of CRF01_AE. The 5 recombinant breakpoints of the 2 URFs were quite similar. Distinct from CRF01_AE/CRF07_BC URFs reported before, 2016GXNNIDU037 and 2019QZLSIDU253 are new evidence of a high genetic variety of HIV-1 in Guangxi, which may pose new challenges to HIV-1 prevention and molecular epidemiological surveillance in China.

Tracing the origin and history of HIV-1 subtype B′ epidemic by near full-length genome analyses

AIDS ◽  
2012 ◽  
Vol 26 (7) ◽  
pp. 877-884 ◽  
Author(s):  
Zhe Li ◽  
Xiang He ◽  
Zhe Wang ◽  
Hui Xing ◽  
Fan Li ◽  
...  
Keyword(s):  
Full Length ◽  
Subtype B ◽  
History Of ◽  
Genome Analyses ◽  
Full Length Genome ◽  
Hiv 1

scholarly journals Evolutionary History of HIV-1 Subtype B and CRF01_AE Transmission Clusters among Men Who Have Sex with Men (MSM) in Kuala Lumpur, Malaysia

PLoS ONE ◽  
2013 ◽  
Vol 8 (6) ◽  
pp. e67286 ◽  
Author(s):  
Kim Tien Ng ◽  
Lai Yee Ong ◽  
Sin How Lim ◽  
Yutaka Takebe ◽  
Adeeba Kamarulzaman ◽  
...  
Keyword(s):  
Evolutionary History ◽  
Kuala Lumpur ◽  
Subtype B ◽  
History Of ◽  
Sex With Men ◽  
Hiv 1

scholarly journals Demographic and behavioral determinants of self-reported history of sexually-transmitted diseases (STDs) among young migrant men who have sex with men (MSM) in Beijing, China

2011 ◽  
Vol 71 (6) ◽  
pp. 699-708
Author(s):  
Yan Song ◽  
Xiaoming Li ◽  
Liying Zhang ◽  
Yingjie Liu ◽  
Shulin Jiang ◽  
...  
Keyword(s):  
Logistic Regression ◽  
Hiv Prevention ◽  
Sexual Partners ◽  
Intervention Programs ◽  
Multivariate Logistic Regression ◽  
Prevention Intervention ◽  
Hiv Prevention Intervention ◽  
Sexually Transmitted ◽  
History Of ◽  
Sex With Men

Background: Sexually-transmitted disease (STD) is a facilitating cofactor that contributes to human immunodeficiency virus (HIV) transmission. Previous studies indicated a high prevalence of STDs among men who have sex with men (MSM) in China. To date, limited data are available for correlates of STD infection among young migrant MSM in China. The current study intends to examine the association between demographic and behavioral factors and STD infection. Methods: Data were collected from a sample of 307 migrant MSM aged 18–29 years in Beijing in 2009. Multivariate logistic regression analysis was employed to examine the factors associated with a history of STDs. Results: Results showed that about 13% of MSM reported a history of STDs. The average number of lifetime sexual partners was 15.0. About 56.7% of MSM did not use a condom at the first sexual encounter. The percentage of MSM who used a condom consistently was 47.4%. Multivariate logistic regression results showed that STD history was positively associated with education, income, the number of lifetime male sexual partners, and negatively associated with the frequency of properly using a condom. Conclusion: STD history was associated with socioeconomic status and sexual risk behaviors. The results suggested that HIV prevention intervention programs need to educate MSM to reduce the number of sexual partners and to use a condom properly. Furthermore, HIV prevention intervention programs among MSM need to be tailored to meet the needs of different socioeconomic groups of MSM.

scholarly journals Prevalence of HIV and sexually transmitted and blood-borne infections, and related preventive and risk behaviours, among gay, bisexual and other men who have sex with men in Montreal, Toronto and Vancouver: results from the Engage Study

2021 ◽  
Author(s):  
Trevor A. Hart ◽  
David M. Moore ◽  
Syed W. Noor ◽  
Nathan Lachowsky ◽  
Daniel Grace ◽  
...  
Keyword(s):  
Scale Up ◽  
Hiv Prevalence ◽  
Risk Behaviours ◽  
Syphilis Infection ◽  
Sexually Transmitted ◽  
The Past ◽  
Sti Testing ◽  
History Of ◽  
Sex With Men ◽  
Exposure Prophylaxis

Abstract Objectives The last Canadian biobehavioural surveillance study of HIV and other sexually transmitted and blood-borne infections (STBBI) among gay, bisexual and other men who have sex with men (GBM) was conducted in 2010. We designed a study to measure STBBI prevalence among GBM in metropolitan Montreal, Toronto and Vancouver and to document related preventive and risk behaviours. Methods The Engage Cohort Study used respondent-driven sampling (RDS) to recruit GBM who reported sex with another man in the past 6 months. At baseline, we examined recruitment characteristics of the samples, and the RDS-II-adjusted distributions of socio-demographics, laboratory-confirmed HIV and other STBBI prevalence, and related behaviours, with a focus on univariate differences among cities. Results A total of 2449 GBM were recruited from February 2017 to August 2019. HIV prevalence was lower in Montreal (14.2%) than in Toronto (22.2%) or Vancouver (20.4%). History of syphilis infection was similar across cities (14–16%). Vancouver had more HIV-negative/unknown participants who reported never being HIV tested (18.6%) than Toronto (12.9%) or Montreal (11.5%). Both Montreal (74.9%) and Vancouver (78.8%) had higher proportions of men who tested for another STBBI in the past 6 months than Toronto (67.4%). Vancouver had a higher proportion of men who used pre-exposure prophylaxis (PrEP) in the past 6 months (18.9%) than Toronto (11.1%) or Montreal (9.6%). Conclusion The three largest cities of Canada differed in HIV prevalence, STBBI testing and PrEP use among GBM. Our findings also suggest the need for scale-up of both PrEP and STI testing among GBM in Canada.

NGS combined with phylogenetic analysis to detect HIV-1 dual infection in Romanian people who inject drugs

2018 ◽  
Vol 20 (5) ◽  
pp. 308-311 ◽  
Author(s):  
Bogdan Popescu ◽  
Leontina Banica ◽  
Ionelia Nicolae ◽  
Eugen Radu ◽  
Iulia Niculescu ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
People Who Inject Drugs ◽  
Dual Infection ◽  
Hiv 1
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