scholarly journals In Vivo Confocal Microscopic Evaluation of Previously Neglected Oval Cells in Corneal Nerve Vortex: An Inflammatory Indicator of Dry Eye Disease

2021 ◽  
Author(s):  
Dalan Jing ◽  
Xiaodan Jiang ◽  
Yilin Chou ◽  
Shanshan Wei ◽  
Ran Hao ◽  
...  
Keyword(s):  
Peripheral Nerve ◽  
Dry Eye ◽  
Inflammatory Cells ◽  
Average Density ◽  
Dry Eye Disease ◽  
Eye Disease ◽  
Oval Cells ◽  
Oval Cell ◽  
Microscopic Evaluation

Abstract To investigate association of a type of previously neglected oval cell that located in corneal vortex with dry eye disease (DED). Observational, prospective study of 168 patients with different degrees of DED. In vivo confocal microscopy was used to observe the corneal sub-basal nerves and Langerhans cells (LCs) in both corneal vortex and periphery. The bright and oval cells also be inspected in corneal vortex. An artificial intelligence (AI) technique generated the sub-basal nerve fibre parameters. Patients were divided into three different groups based on existence of inflammatory cells. Group 2 patients showed a significant increase in corneal peripheral nerve maximum length and corneal peripheral nerve average density. Patients in Group 3 had more LC numbers than others. A type of bright and oval cell was identified in the corneal vortex might be a type of immature LC and related to disease severity of DED.

scholarly journals Corneal Epithelial Immune Dendritic Cell Alterations in Subtypes of Dry Eye Disease: A Pilot In Vivo Confocal Microscopic Study

2015 ◽  
Vol 56 (12) ◽  
pp. 7179 ◽  
Author(s):  
Ahmad Kheirkhah ◽  
Raheleh Rahimi Darabad ◽  
Andrea Cruzat ◽  
Amir Reza Hajrasouliha ◽  
Deborah Witkin ◽  
...  
Keyword(s):  
Dendritic Cell ◽  
Microscopic Study ◽  
Dry Eye ◽  
Dry Eye Disease ◽  
Eye Disease ◽  
Corneal Epithelial ◽  
Cell Alterations ◽  
Confocal Microscopic Study

scholarly journals Manganese(III) tetrakis(1-methyl-4-pyridyl) porphyrin, a superoxide dismutase mimetic, reduces disease severity in in vitro and in vivo models for dry-eye disease

2019 ◽  
Vol 17 (2) ◽  
pp. 257-264 ◽  
Author(s):  
Agnė Žiniauskaitė ◽  
Symantas Ragauskas ◽  
Anita K. Ghosh ◽  
Rubina Thapa ◽  
Anne E. Roessler ◽  
...  
Keyword(s):  
Superoxide Dismutase ◽  
Disease Severity ◽  
Dry Eye ◽  
Dry Eye Disease ◽  
Eye Disease ◽  
In Vivo Models

scholarly journals Corneal Nerve Regeneration after Self-Retained Cryopreserved Amniotic Membrane in Dry Eye Disease

2017 ◽  
Vol 2017 ◽  
pp. 1-10 ◽  
Author(s):  
Thomas John ◽  
Sean Tighe ◽  
Hosam Sheha ◽  
Pedram Hamrah ◽  
Zeina M. Salem ◽  
...  
Keyword(s):  
Nerve Regeneration ◽  
Dry Eye ◽  
Amniotic Membrane ◽  
Signs And Symptoms ◽  
Dry Eye Disease ◽  
Eye Disease ◽  
Study Group ◽  
Corneal Sensitivity ◽  
Corneal Nerve

Purpose. To evaluate the efficacy of self-retained cryopreserved amniotic membrane (CAM) in promoting corneal nerve regeneration and improving corneal sensitivity in dry eye disease (DED).Methods. In this prospective randomized clinical trial, subjects with DED were randomized to receive CAM (study group) or conventional maximum treatment (control). Changes in signs and symptoms, corneal sensitivity, topography, and in vivo confocal microscopy (IVCM) were evaluated at baseline, 1 month, and 3 months.Results. Twenty subjects (age 66.9 ± 8.9) were enrolled and 17 completed all follow-up visits. Signs and symptoms were significantly improved in the study group yet remained constant in the control. IVCM showed a significant increase in corneal nerve density in the study group (12,241 ± 5083 μm/mm2at baseline, 16,364 ± 3734 μm/mm2at 1 month, and 18,827 ± 5453 μm/mm2at 3 months,p=0.015) but was unchanged in the control. This improvement was accompanied with a significant increase in corneal sensitivity (3.25 ± 0.6 cm at baseline, 5.2 ± 0.5 cm at 1 month, and 5.6 ± 0.4 cm at 3 months,p<0.001) and corneal topography only in the study group.Conclusions. Self-retained CAM is a promising therapy for corneal nerve regeneration and accelerated recovery of the ocular surface health in patients with DED. The study is registered at clinicaltrials.gov with trial identifier:NCT02764814.

scholarly journals The effect of Polydatin in dry eye disease in vivo and in vitro

2019 ◽  
Vol 33 (S1) ◽  
Author(s):  
Bongkyun Park ◽  
Tae Gu Lee ◽  
Soo‐Wang Hyun ◽  
Kyuhyung Jo ◽  
Ik Soo Lee ◽  
...  
Keyword(s):  
Dry Eye ◽  
Dry Eye Disease ◽  
Eye Disease

scholarly journals Advances in the Noninvasive Diagnosis of Dry Eye Disease

2021 ◽  
Vol 11 (21) ◽  
pp. 10384
Author(s):  
Luca Di Cello ◽  
Marco Pellegrini ◽  
Aldo Vagge ◽  
Massimiliano Borselli ◽  
Lorenzo Ferro Desideri ◽  
...  
Keyword(s):  
Dry Eye ◽  
Anterior Segment ◽  
Tear Film ◽  
Optical Quality ◽  
Dry Eye Disease ◽  
Diagnostic Tools ◽  
Eye Disease ◽  
Non Invasive ◽  
Integrated Devices

Dry eye disease (DED) is a multifactorial disease that represents one of the most common ophthalmologic conditions encountered in everyday clinical practice. Traditional diagnostic tests for DED, such as subjective questionnaires, tear film break-up time and the Schirmer test, are often associated with poor reproducibility and reliability, which make the diagnosis, follow-up, and management of the disease challenging. New advances in imaging technologies enable objective and reproducible measurements of DED parameters, thus making the diagnosis a multimodal imaging-based process. The aim of this review is to summarize all the current and emerging diagnostic tools available for the diagnosis and monitoring of DED, such as non-invasive tear breakup time, thermography, anterior segment optical coherence tomography, meibography, interferometry, in vivo confocal microscopy, and optical quality assessment. Although there is not a gold standard imaging technique, new multi-imaging-integrated devices are precious instruments to help clinicians to better cope with the diagnostic complexity of DED.

scholarly journals Development of Lactoferrin-Loaded Liposomes for the Management of Dry Eye Disease and Ocular Inflammation

Pharmaceutics ◽  
2021 ◽  
Vol 13 (10) ◽  
pp. 1698
Author(s):  
Ana López-Machado ◽  
Natalia Díaz-Garrido ◽  
Amanda Cano ◽  
Marta Espina ◽  
Josefa Badia ◽  
...  
Keyword(s):  
Dry Eye ◽  
Ex Vivo ◽  
Visual Disturbance ◽  
Nasolacrimal Duct ◽  
Dry Eye Disease ◽  
Eye Disease ◽  
Lipid Film ◽  
Anti Inflammatory

Dry eye disease (DED) is a high prevalent multifactorial disease characterized by a lack of homeostasis of the tear film which causes ocular surface inflammation, soreness, and visual disturbance. Conventional ophthalmic treatments present limitations such as low bioavailability and side effects. Lactoferrin (LF) constitutes a promising therapeutic tool, but its poor aqueous stability and high nasolacrimal duct drainage hinder its potential efficacy. In this study, we incorporate lactoferrin into hyaluronic acid coated liposomes by the lipid film method, followed by high pressure homogenization. Pharmacokinetic and pharmacodynamic profiles were evaluated in vitro and ex vivo. Cytotoxicity and ocular tolerance were assayed both in vitro and in vivo using New Zealand rabbits, as well as dry eye and anti-inflammatory treatments. LF loaded liposomes showed an average size of 90 nm, monomodal population, positive surface charge and a high molecular weight protein encapsulation of 53%. Biopharmaceutical behaviour was enhanced by the nanocarrier, and any cytotoxic effect was studied in human corneal epithelial cells. Developed liposomes revealed the ability to reverse dry eye symptoms and possess anti-inflammatory efficacy, without inducing ocular irritation. Hence, lactoferrin loaded liposomes could offer an innovative nanotechnological tool as suitable approach in the treatment of DED.

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