The Centres for Disease Control Light Trap (CDC LT) and The Human Decoy Trap (HDT) Compared to The Human Landing Catch (HLC) for Measuring Anopheles Biting in Rural Tanzania

2021 ◽  
Author(s):  
Isaac Haggai Namango ◽  
Carly Marshall ◽  
Adam Saddler ◽  
Amanda Ross ◽  
David Kaftan ◽  
...  
Keyword(s):  
Disease Control ◽  
Large Scale ◽  
Geometric Mean ◽  
Light Trap ◽  
Malaria Vectors ◽  
Human Landing Catch ◽  
Mosquito Abundance ◽  
Rural Tanzania ◽  
Human Landing ◽  
Control Light

Abstract BackgroundThe intensity of vector mosquito biting is an important measure for malaria epidemiology and control. The human landing catch (HLC) is an effective entomological surveillance tool, but is labour-intensive, expensive and raises safety issues. The Centres for Disease Control light trap (CDC LT) and the human decoy trap (HDT) are less costly and exposure-free alternatives. This study compared the CDC LT and HDT against the HLC for measuring Anopheles (An.) biting in rural Tanzania and assessed their suitability as HLC proxies.MethodsIndoor mosquito surveys using HLC and CDC LT and outdoor surveys using HLC and HDT were conducted in 2017 and in 2019 in Ulanga, Tanzania in 19 villages, with one trap per house per night. Species composition, sporozoite rates and the numbers of mosquitoes caught by different trap types were compared. Aggregating the data by village and month, the Bland-Altman approach was used to assess agreement. ResultsOverall, 66,807 Anopheles funestus and 14,606 An. arabiensis adult females were caught from 6,013 CDC LT, 339 indoor HLC, 136 HDT and 195 outdoor HLC collections. Overall, the CDC LT caught fewer malaria vectors than indoor HLC: An. arabiensis (Adjusted rate ratio (Adj.RR) =0.35 (95% confidence interval (CI):0.27-0.46)) and An. funestus (Adj.RR=0.63(95%CI:0.51-0.79)). HDT caught fewer malaria vectors than outdoor HLC: An. arabiensis (Adj.RR=0.04(95%CI:0.01-0.14)) and An. funestus (Adj.RR=0.10(95%CI:0.07-0.15)). The bias and variability of the ratios of geometric mean mosquitoes caught by CDC LT and HDT relative to HLC collections for the same village-month were dependent on mosquito densities. The relative efficacies of both CDC LT and HDT declined with mosquito abundance. The variability in the ratios was substantial for low HLC counts and decreased as mosquito abundance increased. CDCLT caught a higher proportion of infected An. arabiensis and An. funestus than HLC, and HDT caught no infected mosquitoes.ConclusionsIf caution is taken in appreciation of its limitations, the CDC LT is suitable for use in routine entomological surveys and may be preferable for measuring sporozoite rates for Afrotropical mosquitoes. Use of HLC is useful to estimate human exposure to mosquitoes for estimating Entomological Inoculation Rate (EIR). The present design of the HDT is not amenable for use to conduct large-scale entomological surveys.

scholarly journals Evaluation of mosquito electrocuting traps as a safe alternative to the human landing catch for measuring human exposure to malaria vectors in Burkina Faso

2019 ◽  
Vol 18 (1) ◽  
Author(s):  
Antoine Sanou ◽  
W. Moussa Guelbéogo ◽  
Luca Nelli ◽  
K. Hyacinth Toé ◽  
Soumanaba Zongo ◽  
...  
Keyword(s):  
Species Composition ◽  
Burkina Faso ◽  
Malaria Vector ◽  
Human Exposure ◽  
Infected Mosquito ◽  
Malaria Vectors ◽  
Human Landing Catch ◽  
Mosquito Vector ◽  
Human Landing ◽  
Biting Behaviour

Abstract Background Measuring human exposure to mosquito bites is a crucial component of vector-borne disease surveillance. For malaria vectors, the human landing catch (HLC) remains the gold standard for direct estimation of exposure. This method, however, is controversial since participants risk exposure to potentially infected mosquito bites. Recently an exposure-free mosquito electrocuting trap (MET) was developed to provide a safer alternative to the HLC. Early prototypes of the MET performed well in Tanzania but have yet to be tested in West Africa, where malaria vector species composition, ecology and behaviour are different. The performance of the MET relative to HLC for characterizing mosquito vector population dynamics and biting behaviour in Burkina Faso was evaluated. Methods A longitudinal study was initiated within 12 villages in Burkina Faso in October 2016. Host-seeking mosquitoes were sampled monthly using HLC and MET collections over 14 months. Collections were made at 4 households on each night, with METs deployed inside and outside at 2 houses, and HLC inside and outside at another two. Malaria vector abundance, species composition, sporozoite rate and location of biting (indoor versus outdoor) were recorded. Results In total, 41,800 mosquitoes were collected over 324 sampling nights, with the major malaria vector being Anopheles gambiae sensu lato (s.l.) complex. Overall the MET caught fewer An. gambiae s.l. than the HLC (mean predicted number of 0.78 versus 1.82 indoors, and 1.05 versus 2.04 outdoors). However, MET collections gave a consistent representation of seasonal dynamics in vector populations, species composition, biting behaviour (location and time) and malaria infection rates relative to HLC. As the relative performance of the MET was somewhat higher in outdoor versus indoor settings, this trapping method slightly underestimated the proportion of bites preventable by LLINs compared to the HLC (MET = 82.08%; HLC = 87.19%). Conclusions The MET collected proportionately fewer mosquitoes than the HLC. However, estimates of An. gambiae s.l. density in METs were highly correlated with HLC. Thus, although less sensitive, the MET is a safer alternative than the HLC. Its use is recommended particularly for sampling vectors in outdoor environments where it is most sensitive.

scholarly journals Low-density genetically diverse natural Wolbachia infections in malaria vectors in Kayin state, Myanmar

2019 ◽  
Vol 4 ◽  
pp. 11
Author(s):  
Sunisa Sawasdichai ◽  
Victor Chaumeau ◽  
Tee Dah ◽  
Thithiworada Kulabkeeree ◽  
Ladda Kajeechiwa ◽  
...  
Keyword(s):  
Malaria Vectors ◽  
Human Landing Catch ◽  
Low Density ◽  
Mosquito Vectors ◽  
Infection Rates ◽  
Vector Species ◽  
Closely Related Species ◽  
Human Landing ◽  
Malaria Mosquitoes ◽  
Rrna Sequences

Background: Natural Wolbachia infections in malaria mosquitoes were recently reported in Africa, and negatively correlated with the development of Plasmodium falciparum in the vectors. The occurrence and effects of Wolbachia infections outside Africa have not been described and may have been underestimated. Methods: Mosquitoes were collected by human-landing catch during May and June 2017 in ten villages in Kayin state, Myanmar. Closely related species of malaria vectors were identified with molecular assays. Wolbachia infection rates were assessed with quantitative real-time PCR. Results: Low titer of Wolbachia DNA was detected in 13/370 samples in six malaria vector species. Phylogenetic analysis based on 16S rRNA sequences revealed a high diversity of Wolbachia strains and identified lineages different from those described in the African malaria mosquitoes. Conclusion: These low-density genetically diverse natural Wolbachia infections question the ecology and biology of Wolbachia-Anopheles interactions in Southeast Asia. Their effects on malaria transmission and mosquito vectors are yet to be determined.

scholarly journals Semi Field Evaluation of Freestanding Transfluthrin Passive Emanators and the BG Sentinel Trap as a “Push-Pull control Strategy” Against Aedes aegypti Mosquitoes

2020 ◽  
Author(s):  
Mgeni Mohamed Tambwe ◽  
Sarah Moore ◽  
Hassan Chilumba ◽  
Johnson Kyeba Swai ◽  
Jason Moore ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Field Experiments ◽  
Protective Efficacy ◽  
Malaria Vectors ◽  
Human Landing Catch ◽  
Regular User ◽  
Pull System ◽  
Significant Difference ◽  
Human Landing ◽  
Bg Sentinel

Abstract Background The use of volatile pyrethroids and odor-baited traps in a push-pull system has been shown to reduce house entry and outdoor bites for malaria vectors. This technology has the potential to control other outdoor biting mosquitoes such as Aedes aegypti that transmit arboviral diseases. In this study, semi-field experiments were conducted to evaluate whether a push-pull system could be used to reduce bites from Aedes.Methods The push and pull under investigation consisted of two freestanding transfluthrin passive emanators (FTPE), and a BG sentinel trap (BGS) respectively. The FTPE contained hessian strips treated with 5.25 g of transfluthrin active ingredient. The efficacies of FTPE and BGS alone and in combination were evaluated by human landing catch in a large semi-field system in Tanzania. We also investigated the protection of FTPE over six months. The data was analyzed using generalized linear mixed models with binomial distribution.Results Two FTPE have a protective efficacy (PE) of 61% (95% Confidence interval (CI): 52.18–69.91) against human landing rate of Aedes aegypti. The BGS did not significantly reduce mosquito landings; the PE was 2.14% (95% CI: -2.87-7.16). The combination of FTPE and BGS (push-pull) provided the PE of 64.46% (95% CI: 59.06–69.85). However, there was no significant difference in the protective efficacy between the push-pull and the two FTPE against Ae. aegypti (p = 0.286). The FTPE offered significant protection against Ae. aegypti at month three, with a PE of 46.44% (95% CI: 41.12–51.76), but not at six months with a PE of 2.20% (95% CI: -9.00-14.02).Conclusions The protective efficacy of the FTPE and the full push-pull system are similar, indicative that bite prevention is primarily due to the activity of the FTPE. While these results are encouraging for the FTPE, further work is needed for a push-pull system to be recommended for Ae. aegypti control. The three-month protection provided by the FTPE against Ae. aegypti bites suggests it would be a useful additional personal protection tool during dengue outbreaks, that does not require regular user compliance.

scholarly journals New insights into malaria vector bionomics in Lao PDR: a nationwide entomology survey

2020 ◽  
Author(s):  
Sebastien Marcombe ◽  
Santi Maithaviphet ◽  
Julie Bobichon ◽  
Nothasin Phommavan ◽  
Simone Nambanya ◽  
...  
Keyword(s):  
Molecular Identification ◽  
Sibling Species ◽  
Rural Areas ◽  
Lao Pdr ◽  
Malaria Vectors ◽  
Human Landing Catch ◽  
Morphological Identification ◽  
Anopheles Mosquitoes ◽  
Malaria Burden ◽  
Human Landing

Abstract Background . In Laos, the malaria burden remains high despite a significant reduction of cases during the last decade. In the context of the disease elimination by 2030, a nationwide entomological survey was conducted to better understand the distribution, abundance and behavior of major malaria vectors ( Anopheles spp.) in the country. Methods . Mosquito collections were implemented in ten villages from ten provinces during the rainy and dry seasons of 2014 and 2015 by using human landing catch (HLC) and cow bait collection (CBC) methods. After morphological identification in the field, molecular identification of the sibling species of Anopheles mosquitoes from the Funestus, Leucosphyrus, and Maculatus groups were determined using PCR specific alleles. A screening of Plasmodium falciparum and P. vivax infections in the vectors was carried out by quantitative PCR assays. Results. A total of 14,146 adult mosquitoes representing 25 different Anopheles species were collected and morphologically identified. Molecular identification revealed the presence of 12 sibling species within the main primary vector groups including An. maculatus s.s., An. rampae , An. sawadwongporni , An. pseudowillmori , An. dravidicus , An. minimus s.s., An. aconitus , An. pampanai , An. harrisoni , An. dirus s.s., An. baimaii , An. nemophilous . Anopheles maculatus and An. minimus were predominant during both the dry and rainy seasons, but showed highly zoophilic preferences (Zoophilic index of 98% and 95%, respectively). Overall, 22% of the total malaria vectors were collected between 10:00 PM and 5:00 AM indoors when people are sleeping. Twenty-seven percent of primary and secondary vectors were collected outdoors before 10:00 PM or after 5:00 AM, times when people are usually awake and outdoors. Only two specimens were positive for P. falciparum , one An. aconitus from Phongsaly and one An. minimus from Vientiane Province Conclusions. The results indicate that people living in rural areas in Laos are constantly exposed to malaria vectors throughout the year and specifically outdoors. The use of LLINs/IRS remains important but innovative tools and new strategies are needed to address locally, the early and outdoor malaria transmission. Lack of expertise in general entomological methods may further exacerbate the situation.

scholarly journals New insights into malaria vector bionomics in Lao PDR: a nationwide entomology survey

2020 ◽  
Vol 19 (1) ◽  
Author(s):  
Sébastien Marcombe ◽  
Santi Maithaviphet ◽  
Julie Bobichon ◽  
Nothasin Phommavan ◽  
Simone Nambanya ◽  
...  
Keyword(s):  
Molecular Identification ◽  
Sibling Species ◽  
Rural Areas ◽  
Lao Pdr ◽  
Malaria Vectors ◽  
Human Landing Catch ◽  
Morphological Identification ◽  
Anopheles Dirus ◽  
Malaria Burden ◽  
Human Landing

Abstract Background In Laos, the malaria burden remains high despite a significant reduction of cases during the last decade. In the context of the disease elimination by 2030, a nationwide entomological survey was conducted to better understand the distribution, abundance and behaviour of major malaria vectors (Anopheles spp.) in the country. Methods Mosquito collections were implemented in ten villages from ten provinces during the rainy and dry seasons of 2014 and 2015 by using human landing catch (HLC) and cow bait collection (CBC) methods. After morphological identification in the field, molecular identification of the sibling species of Anopheles mosquitoes from the Funestus, Leucosphyrus, and Maculatus groups were determined using PCR specific alleles. A screening of Plasmodium falciparum and Plasmodium vivax infections in the vectors was carried out by quantitative PCR assays. Results A total of 14,146 adult mosquitoes representing 25 different Anopheles species were collected and morphologically identified. Molecular identification revealed the presence of 12 sibling species within the main primary vector groups, including Anopheles maculatus, Anopheles rampae, Anopheles sawadwongporni, Anopheles pseudowillmori, Anopheles dravidicus, Anopheles minimus, Anopheles aconitus, Anopheles pampanai, Anopheles harrisoni, Anopheles dirus, Anopheles baimaii, Anopheles nemophilous. Anopheles maculatus and An. minimus were predominant during both the dry and rainy seasons, but showed highly zoophilic preferences (Zoophilic index of 98% and 95%, respectively). Overall, 22% of the total malaria vectors were collected between 10:00 PM and 5:00 AM indoors when people are sleeping. Twenty-seven percent of primary and secondary vectors were collected outdoors before 10:00 PM or after 5:00 AM, times when people are usually awake and outdoors. Only two specimens were positive for P. falciparum, one An. aconitus from Phongsaly and one An. minimus from Vientiane Province Conclusions The results indicate that people living in rural areas in Laos are constantly exposed to malaria vectors throughout the year and specifically outdoors. The use of LLINs/IRS remains important but innovative tools and new strategies are needed to address locally, the early and outdoor malaria transmission. Lack of expertise in general entomological methods may further exacerbate the situation.

scholarly journals Development and evaluation of mosquito-electrocuting traps as alternatives to the human landing catch technique for sampling host-seeking malaria vectors

2015 ◽  
Vol 14 (1) ◽  
Author(s):  
Deodatus V. Maliti ◽  
Nicodem J. Govella ◽  
Gerry F. Killeen ◽  
Nosrat Mirzai ◽  
Paul C. D. Johnson ◽  
...  
Keyword(s):  
Malaria Vectors ◽  
Human Landing Catch ◽  
Host Seeking ◽  
Human Landing

scholarly journals Erratum to: Development and evaluation of mosquito-electrocuting traps as alternatives to the human landing catch technique for sampling host-seeking malaria vectors

2016 ◽  
Vol 15 (1) ◽  
Author(s):  
Deodatus V. Maliti ◽  
Nicodem J. Govella ◽  
Gerry F. Killeen ◽  
Nosrat Mirzai ◽  
Paul C. D. Johnson ◽  
...  
Keyword(s):  
Malaria Vectors ◽  
Human Landing Catch ◽  
Host Seeking ◽  
Human Landing

Relationship to human biting collections and influence of light and bednet in CDC light-trap catches of West African malaria vectors

1998 ◽  
Vol 88 (5) ◽  
pp. 503-511 ◽  
Author(s):  
C. Costantini ◽  
N.F. Sagnon ◽  
E. Sanogo ◽  
L. Merzagora ◽  
M. Coluzzi
Keyword(s):  
Light Trap ◽  
West African ◽  
Malaria Vectors ◽  
Prototype Model ◽  
Light Traps ◽  
Mosquito Net ◽  
Cdc Light Trap ◽  
Incandescent Bulb ◽  
Human Landing ◽  
Trap Catches

AbstractThe efficiency of miniature CDC light-traps in catching West African malaria vectors was evaluated during two rainy seasons in a village near Ouagadougou, Burkina Faso. Traps were employed both indoors and outdoors using human baits protected by an insecticide-free mosquito-net and different sources of light. Indoors, light from incandescent bulbs increased the catch of Anopheles gambiae s.l. (mainly A. arabiensis Patton and the Mopti chromosomal form of A. gambiae s.s. Giles) and A. funestus Giles c. 2.5 times as compared to traps whose light bulb was removed. Conversely, the difference was not significant when a UV ‘Blacklight-blue’ fluorescent tube was compared to the incandescent bulb. Protecting the bait with a mosquito-net increased the catch c. 3 times for A. gambiae s.l. and c. 3.5 times for A. funestus. A prototype model of double bednet gave intermediate yields. Outdoors, the addition of incandescent bulbs to unlighted traps did not significantly increase the number of vectors caught, but the addition of the mosquito-net to the unprotected human bait did so by c. 1.5–4 times. Thus, the CDC light-trap hung close to a human sleeping under a bednet and fitted with an incandescent bulb, was considered the most practical and efficient in terms of numbers of vectors caught, consequently its indoor efficiency was compared to human landing catches on single collectors and estimated to be 1.08 times and density-independent. Outdoor light-trap catches were either not significantly correlated to biting collections (as for A. gambiae s.l.), or density-dependent in their efficiency (as for A. funestus); thus, they were not considered a reliable means for estimating malaria vector outdoor biting densities in this area. No difference was found in the parous rate of A. gambiae s.l. samples obtained with CDC light-traps and human landing collections.

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