scholarly journals A Novel Mouse Model to Study Fracture Healing of Tibia

2022 ◽  
Author(s):  
Xiangyang Leng ◽  
Jianyu Wang ◽  
Zhenwei Zhou ◽  
Yuyan Jia ◽  
Yuchi Wei ◽  
...  
Keyword(s):  
Mouse Model ◽  
Fracture Healing ◽  
Intramedullary Nail ◽  
Gait Pattern ◽  
Delayed Union ◽  
Fracture Model ◽  
Open Approach ◽  
X Ray ◽  
Intramedullary Stabilization ◽  
High Repeatability

Abstract Background Delayed union of most tibial fractures due to their special anatomical structures.So an effective animal model is very important to study the mechanism and method of fracture healing.However, due to the small tibia of mice, the operation is difficult, and the surgical model requires high surgical skills. The construction of the fixation model of intramedullary nail for this fracture has improved and simplified the traditional fixation model of intramedullary nail, which not only achieves the purpose of constructing the fracture model, but also makes it more simple and effective.Therefore, the aim of the current study was to develop a new mouse model to study fracture healing of tibia. Methods We chose a combination between an open osteotomy and intramedullary stabilization. The 22G needle was inserted into the fracture end in a closed manner by using an open approach for osteotomy at the middle and lower 1/3 level of the tibia.Fractured tibia were analyzed using microcomputed tomography and histology at days 7,14,21and 28after surgery. All animals displayed normal limb loading and a physio-logical gait pattern within the first three days after fracture. No animals were lost due to surgery or anesthesia. Results X-ray confirmed that the fracture types obtained by the fracture modeling method were transverse fractures. X-ray, Micro-CT, immunohistochemistry, histological staining and Real-time PCR showed that the fracture healing of mice was typical endochondral ossification, with high repeatability. Conclusion The mouse tibial fracture model established by intramedullary nailing is safe, rapid and simple. Its fracture healing is a typical intrachondral ossification with high repeatability, which can be better used for the study of molecular mechanism and clinical transformation of fracture healing and bone metabolism.

scholarly journals Ischemia leads to delayed union during fracture healing: A mouse model

2006 ◽  
Vol 25 (1) ◽  
pp. 51-61 ◽  
Author(s):  
Chuanyong Lu ◽  
Theodore Miclau ◽  
Diane Hu ◽  
Ralph S. Marcucio
Keyword(s):  
Mouse Model ◽  
Fracture Healing ◽  
Delayed Union

scholarly journals OP-1 Augments Glucocorticoid-inhibited Fracture Healing in a Rat Fracture Model

2009 ◽  
Vol 467 (12) ◽  
pp. 3104-3112 ◽  
Author(s):  
Robert S. Gilley ◽  
Larry J. Wallace ◽  
Craig A. Bourgeault ◽  
Louis S. Kidder ◽  
Joan E. Bechtold
Keyword(s):  
Fracture Healing ◽  
Fracture Model

Dual Role of Caveolin-1 in β-Catenin Signaling During Fracture Healing Induced by Low-Intensity Pulsed Ultrasound in Rabbits

2021 ◽  
Vol 11 (2) ◽  
pp. 229-239
Author(s):  
Yun Li ◽  
Guanghua Liu ◽  
Feng Xiao ◽  
Wenqin Gu ◽  
Zhengdong Gao ◽  
...  
Keyword(s):  
Fracture Healing ◽  
Molecular Mechanisms ◽  
Dual Role ◽  
Fracture Repair ◽  
Long Bone ◽  
Pulsed Ultrasound ◽  
Mineral Density ◽  
X Ray ◽  
Caveolin 1 ◽  
The Right

We did this research to observe the effect of LIPUS on long bone fracture repair and caveolin-1, β-catenin signaling expression in the radius defects of rabbits, to explore its possible molecular mechanisms. 24 male New Zealand rabbits with bilateral radial bone defects were divided into 4 groups randomly, n = 6. The right side had daily LIPUS exposure for 20 minutes, while the left received sham treatment. After 7, 14, 21, 28 days, respectively, fracture healing was observed by X-ray imaging and Dual Energy X-ray Absorptiometry (DXA) scan, specimens were harvested for histology, immunohistochemistry, and gene expression analysis. We found that LIPUS brought forward endochondral ossification, increased the bone callus size without changes in Bone Mineral Density (BMD). The caveolin-1 expression increased first then decreased, while the β-catenin kept growing during the process. These demonstrated that caveolin-1 participated in fracture healing accelerated by LIPUS, which was speculated to play a dual role in β-catenin signaling expression.

scholarly journals Melatonin promotes fracture healing at pharmacological doses on the early phase in perforating fracture model

2013 ◽  
Vol 27 (S1) ◽  
Author(s):  
Koo Kim ◽  
Seunghoon Lee ◽  
Yunkyung Hong ◽  
Minkyung Lee ◽  
Yonggeun Hong
Keyword(s):  
Fracture Healing ◽  
Early Phase ◽  
Fracture Model

scholarly journals Regulation of osteoblast autophagy based on PI3K/AKT/mTOR signaling pathway study on the effect of β-ecdysterone on fracture healing

2021 ◽  
Author(s):  
Yanghua Tang ◽  
Yafeng Mo ◽  
Dawei Xin ◽  
Zhenfei Xiong ◽  
Linru Zeng ◽  
...  
Keyword(s):  
Western Blot ◽  
Fracture Healing ◽  
Underlying Mechanism ◽  
Immunofluorescence Assay ◽  
The State ◽  
Expression Level ◽  
Pathological State ◽  
Mtor Signaling Pathway ◽  
X Ray ◽  
Alp Activity

Purpose: To investigate the effects of β-ecdysterone on fracture healing and the underlying mechanism. Methods: MTT assay was used to detect the cell viability and alkaline phosphatase (ALP) activity was measured using a commercial kit. AO/PI and flow cytometry assays were used to determine the state of apoptosis of osteoblasts. The expression level of RunX2, ATG7 and LC3 was evaluated by qRT-PCR and Western blot assays. X-ray and HE staining were conducted on the fractured femur to evaluate the pathological state. Immunohistochemical assay was used to detect the expression level of Beclin-1 and immunofluorescence assay was used to measure the expression level of LC3 in the fractured femurs. Western blot was utilized to determine the expression level of PI3K, p-AKT1, AKT1, p-mTOR, mTOR, p-p70S6K, and p70S6K. Results: The ALP activity and expression of RunX2 in fractured osteoblasts were significantly suppressed by 3-methyladenine and elevated by rapamycin, 60, and 80 μM β-ecdysterone. The apoptotic state of fractured osteoblasts was enhanced by 3-methyladenine and alleviated by rapamycin, 60, and 80 μM β-ecdysterone. The state of autophagy both in fractured osteoblasts and femurs was inhibited by 3-methyladenine and facilitated by rapamycin and β-ecdysterone. Compared to control, Garrett score in 3-methyladenine group was significantly decreased and promoted in rapamycin and β-ecdysterone groups, accompanied by ameliorated pathological state. Lastly, the PI3K/AKT/mTOR pathway both in fractured osteoblasts and femurs was activated by 3-methyladenine and inhibited by rapamycin and β-ecdysterone. Conclusion: β-ecdysterone might facilitate fracture healing by activating autophagy through suppressing PI3K/AKT/mTOR signal pathway.

scholarly journals Comparison of Conservative and Operative Treatment of Jones Fracture

2019 ◽  
Author(s):  
Jiri Demel ◽  
Ladislav Planka ◽  
Radek Stichhauer ◽  
Leopold Pleva
Keyword(s):  
Surgical Treatment ◽  
Fracture Healing ◽  
Conservative Therapy ◽  
Aofas Score ◽  
X Ray ◽  
Jones Fracture ◽  
Conservative And Operative Treatment ◽  
O 15 ◽  
K 12 ◽  
Type Of Fracture

Abstract Background: A Jones fracture is a transverse fracture of the V. metatarsal bone, without significant dislocation in the diaphysis junction and metaphyseal metaphysis. This type of fracture is usually associated with a minimal injury mechanism, and is characterized with minimal swelling, absence of hematoma and prolonged healing. The aim of the presented study was to compare the surgical and conservative therapy of Jones fracture. The study was registered in the www.clinicaltrials.gov database, under the ClinicalTrials.gov ID: NCT04037540 on 27th July 2019. Methods: A total of 27 study subjects were randomized into two groups – Conservative (K): 12, and Operational (O): 15. The study subjects were followed after 6 and 12 weeks using X-ray and the American Orthopedic Foot and Ankle Score (AOFAS). In the absence of X-ray signs of healing and low AOFAS score in Group K, treatment was considered unsuccessful and the patient was indicated for surgical treatment. Results: Five patients in Group K showed no signs of healing on X-ray after 12 to 6 weeks. These patients also manifested poor AOFAS scores and were indicated for surgical treatment and excluded from the study. Fracture failure was observed in seven patients. Their AOFAS scores were at the least satisfactory and, the patients continued in conservative therapy. After 12 weeks, 6 patients manifested fracture healing on X-ray and the AOFAS score of 100. In one patient who refused surgery, the fracture was not healed after 12 weeks. In Group O, seven patients achieved fracture healing on X-ray after 6 weeks, 8 patients did not show fracture healing. The average AOFAS score after 6 weeks was 97 (excellent). After 12 months, 13 patients showed findings of fracture healing on X-ray. The average AOFAS score was 100 points (maximum). Conclusions: The results of the study show that 1) Surgical treatment leads to significantly faster signs of healing on X-ray than the conservative one 2) After six weeks of surgery, 93% (14 out of 15) of patients are able to fully load the limb. Keywords: Jones - Fracture - AOFAS - Herbert screw Unique protocol ID: FNO-KUCH-01-Jones

A Single-Channel Telemetric Intramedullary Nail for In Vivo Measurement of Fracture Healing

2009 ◽  
Vol 23 (10) ◽  
pp. 702-709 ◽  
Author(s):  
Darren J Wilson ◽  
Robert L Morgan ◽  
Katy L Hesselden ◽  
James R Dodd ◽  
Si W Janna ◽  
...  
Keyword(s):  
Fracture Healing ◽  
Intramedullary Nail ◽  
Single Channel ◽  
In Vivo Measurement
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