scholarly journals Experimental Evidence for Manure-Borne Bacteria Invasion in Soil During a Coalescent Event: Influence of the Antibiotic Sulfamethazine

2022 ◽  
Author(s):  
Loren Billet ◽  
Stéphane Pesce ◽  
Fabrice Martin-Laurent ◽  
Marion Devers-Lamrani
Keyword(s):  
Bacterial Communities ◽  
Soil Microorganisms ◽  
Organic Amendment ◽  
Resistant Bacteria ◽  
Antibiotic Resistant ◽  
Abundant Otus ◽  
Rdna Sequencing ◽  
Different Soils

Abstract The fertilization of agricultural soil by organic amendment that may contain antibiotics, like manure, can transfer bacterial pathogens and antibiotic-resistant bacteria to soil communities. However, the invasion by manure-borne bacteria in amended soil remains poorly understood, being hardly observed. Here, we assessed the invasions of manure-borne bacteria during a coalescence event between manure and soil, in different soils and in the presence or absence of antibiotics. To this end, microcosms of four different soils were amended or not with manure at an agronomical dose and/or exposed or not to the antibiotic sulfamethazine (SMZ). After one month of incubation, the diversity, structure and composition of bacterial communities of the soils were assessed by 16S rDNA sequencing. The invasion of manure-borne bacteria was still perceptible one month after the soil amendment. The results obtained with the soil already amended in situ with manure six months prior to the experiment suggest that some of the bacterial invaders were established in the community over the long term. Even if differences were observed between soils, the invasion was mainly attributable to some of the most abundant OTUs of manure (mainly Firmicutes). SMZ exposure had a limited influence on soil microorganisms. It was significant in only one soil, where it enhanced the invasion potential of some manure-borne invaders.

scholarly journals Long-term in situ permafrost thaw effects on bacterial communities and potential aerobic respiration

2018 ◽  
Vol 12 (9) ◽  
pp. 2129-2141 ◽  
Author(s):  
Sylvain Monteux ◽  
James T. Weedon ◽  
Gesche Blume-Werry ◽  
Konstantin Gavazov ◽  
Vincent E. J. Jassey ◽  
...  
Keyword(s):  
Bacterial Communities ◽  
Aerobic Respiration ◽  
Permafrost Thaw

scholarly journals Plasmid Dynamics in KPC-Positive Klebsiella pneumoniae during Long-Term Patient Colonization

mBio ◽  
2016 ◽  
Vol 7 (3) ◽  
Author(s):  
Sean Conlan ◽  
Morgan Park ◽  
Clayton Deming ◽  
Pamela J. Thomas ◽  
Alice C. Young ◽  
...  
Keyword(s):  
Health Care ◽  
Antibiotic Resistance ◽  
Klebsiella Pneumoniae ◽  
Health Care Facilities ◽  
Resistant Bacteria ◽  
Whole Genome ◽  
Antibiotic Resistant ◽  
Carbapenem Resistant ◽  
Clinical Center

ABSTRACT Carbapenem-resistant Klebsiella pneumoniae strains are formidable hospital pathogens that pose a serious threat to patients around the globe due to a rising incidence in health care facilities, high mortality rates associated with infection, and potential to spread antibiotic resistance to other bacterial species, such as Escherichia coli . Over 6 months in 2011, 17 patients at the National Institutes of Health (NIH) Clinical Center became colonized with a highly virulent, transmissible carbapenem-resistant strain of K. pneumoniae . Our real-time genomic sequencing tracked patient-to-patient routes of transmission and informed epidemiologists’ actions to monitor and control this outbreak. Two of these patients remained colonized with carbapenemase-producing organisms for at least 2 to 4 years, providing the opportunity to undertake a focused genomic study of long-term colonization with antibiotic-resistant bacteria. Whole-genome sequencing studies shed light on the underlying complex microbial colonization, including mixed or evolving bacterial populations and gain or loss of plasmids. Isolates from NIH patient 15 showed complex plasmid rearrangements, leaving the chromosome and the bla KPC -carrying plasmid intact but rearranging the two other plasmids of this outbreak strain. NIH patient 16 has shown continuous colonization with bla KPC -positive organisms across multiple time points spanning 2011 to 2015. Genomic studies defined a complex pattern of succession and plasmid transmission across two different K. pneumoniae sequence types and an E. coli isolate. These findings demonstrate the utility of genomic methods for understanding strain succession, genome plasticity, and long-term carriage of antibiotic-resistant organisms. IMPORTANCE In 2011, the NIH Clinical Center had a nosocomial outbreak involving 19 patients who became colonized or infected with bla KPC -positive Klebsiella pneumoniae . Patients who have intestinal colonization with bla KPC -positive K. pneumoniae are at risk for developing infections that are difficult or nearly impossible to treat with existing antibiotic options. Two of those patients remained colonized with bla KPC -positive Klebsiella pneumoniae for over a year, leading to the initiation of a detailed genomic analysis exploring mixed colonization, plasmid recombination, and plasmid diversification. Whole-genome sequence analysis identified a variety of changes, both subtle and large, in the bla KPC -positive organisms. Long-term colonization of patients with bla KPC -positive Klebsiella pneumoniae creates new opportunities for horizontal gene transfer of plasmids encoding antibiotic resistance genes and poses complications for the delivery of health care.

scholarly journals Correction: Long-term in situ permafrost thaw effects on bacterial communities and potential aerobic respiration

2019 ◽  
Vol 13 (8) ◽  
pp. 2140-2142
Author(s):  
Sylvain Monteux ◽  
James T. Weedon ◽  
Gesche Blume-Werry ◽  
Konstantin Gavazov ◽  
Vincent E. J. Jassey ◽  
...  
Keyword(s):  
Bacterial Communities ◽  
Aerobic Respiration ◽  
Permafrost Thaw

scholarly journals Colonization and Infection with Antibiotic-Resistant Bacteria in a Long-Term Care Facility

1994 ◽  
Vol 42 (10) ◽  
pp. 1062-1069 ◽  
Author(s):  
Margaret S. Terpenning ◽  
Suzanne F. Bradley ◽  
Jim Y. Wan ◽  
Carol E. Chenoweth ◽  
Karen A. Jorgensen ◽  
...  
Keyword(s):  
Long Term Care ◽  
Care Facility ◽  
Resistant Bacteria ◽  
Antibiotic Resistant Bacteria ◽  
Term Care ◽  
Antibiotic Resistant ◽  
Term Care Facility ◽  
Long Term Care Facility

scholarly journals Antibiotrophy: Key Function for Antibiotic-Resistant Bacteria to Colonize Soils—Case of Sulfamethazine-Degrading Microbacterium sp. C448

2021 ◽  
Vol 12 ◽  
Author(s):  
Loren Billet ◽  
Stéphane Pesce ◽  
Nadine Rouard ◽  
Aymé Spor ◽  
Laurianne Paris ◽  
...  
Keyword(s):  
Swine Manure ◽  
Selective Advantage ◽  
Soil Types ◽  
Resistant Bacteria ◽  
Ecological Niches ◽  
Antibiotic Resistant Bacteria ◽  
Microbial Competition ◽  
Antibiotic Resistant ◽  
Rdna Sequencing ◽  
Trophic Niches

Chronic and repeated exposure of environmental bacterial communities to anthropogenic antibiotics have recently driven some antibiotic-resistant bacteria to acquire catabolic functions, enabling them to use antibiotics as nutritive sources (antibiotrophy). Antibiotrophy might confer a selective advantage facilitating the implantation and dispersion of antibiotrophs in contaminated environments. A microcosm experiment was conducted to test this hypothesis in an agroecosystem context. The sulfonamide-degrading and resistant bacterium Microbacterium sp. C448 was inoculated in four different soil types with and without added sulfamethazine and/or swine manure. After 1 month of incubation, Microbacterium sp. (and its antibiotrophic gene sadA) was detected only in the sulfamethazine-treated soils, suggesting a low competitiveness of the strain without antibiotic selection pressure. In the absence of manure and despite the presence of Microbacterium sp. C448, only one of the four sulfamethazine-treated soils exhibited mineralization capacities, which were low (inferior to 5.5 ± 0.3%). By contrast, manure addition significantly enhanced sulfamethazine mineralization in all the soil types (at least double, comprised between 5.6 ± 0.7% and 19.5 ± 1.2%). These results, which confirm that the presence of functional genes does not necessarily ensure functionality, suggest that sulfamethazine does not necessarily confer a selective advantage on the degrading strain as a nutritional source. 16S rDNA sequencing analyses strongly suggest that sulfamethazine released trophic niches by biocidal action. Accordingly, manure-originating bacteria and/or Microbacterium sp. C448 could gain access to low-competition or competition-free ecological niches. However, simultaneous inputs of manure and of the strain could induce competition detrimental for Microbacterium sp. C448, forcing it to use sulfamethazine as a nutritional source. Altogether, these results suggest that the antibiotrophic strain studied can modulate its sulfamethazine-degrading function depending on microbial competition and resource accessibility, to become established in an agricultural soil. Most importantly, this work highlights an increased dispersal potential of antibiotrophs in antibiotic-polluted environments, as antibiotics can not only release existing trophic niches but also form new ones.

scholarly journals Corncob structures in dental plaque reveal microhabitat taxon specificity

2021 ◽  
Author(s):  
Viviana Morillo-Lopez ◽  
Alexandra Sjaarda ◽  
Imon Islam ◽  
Gary G. Borisy ◽  
Jessica Mark Welch
Keyword(s):  
Bacterial Communities ◽  
Dental Plaque ◽  
Spatial Organization ◽  
Physical Distance ◽  
Small Subset ◽  
Long Term Stability ◽  
Natural Laboratory ◽  
Microhabitat Specialization

Abstract Background The human mouth is a natural laboratory for studying how bacterial communities differ across habitats. Different bacteria colonize different surfaces in the mouth – teeth, tongue dorsum, and keratinized and non-keratinized epithelia – despite the short physical distance between these habitats and their connection through saliva. We sought to determine whether more tightly defined microhabitats might have more tightly defined sets of resident bacteria. A microhabitat may be characterized, for example, as the space adjacent to a particular species of bacterium. Corncob structures of dental plaque, consisting of coccoid bacteria bound to filaments of Corynebacterium cells, present an opportunity to analyze the community structure of one such well-defined microhabitat within a complex natural biofilm. Here we investigate by fluorescence in situ hybridization and spectral imaging the composition of the cocci decorating the filaments. Results The range of taxa observed in corncobs was limited to a small subset of the taxa present in dental plaque. Among four major groups of dental plaque streptococci, two were the major constituents of corncobs, including one that was the most abundant Streptococcus species in corncobs despite being relatively rare in dental plaque overall. Images showed both Streptococcus types in corncobs in all individual donors, suggesting that the taxa possess different ecological roles or that mechanisms exist for stabilizing the persistence of functionally redundant taxa in the population. Direct taxon-taxon interactions were observed not only between the Streptococcus cells and the central corncob filament but also between Streptococcus cells and the limited subset of other plaque bacteria detected in the corncobs, indicating microhabitat specialization involving these taxa as well. Conclusions The spatial organization we observed in corncobs suggests that each of the microbial participants is capable of interacting with multiple, albeit limited, potential partners, a feature that may encourage the long-term stability of the community. Additionally, our results suggest the general principle that a precisely defined microhabitat will be inhabited by a small and well-defined set of microbial taxa.

scholarly journals Potential Solutions Using Bacteriophages against Antimicrobial Resistant Bacteria

Antibiotics ◽  
2021 ◽  
Vol 10 (12) ◽  
pp. 1496
Author(s):  
Aryan-Rahimi Midani ◽  
Seon-Woo Lee ◽  
Tae-Jin Choi
Keyword(s):  
Pathogenic Bacteria ◽  
Control Agent ◽  
Future Application ◽  
Resistant Bacteria ◽  
Natural Environments ◽  
The Novel ◽  
Antibiotic Resistant ◽  
Sequencing Technologies ◽  
Crucial Information

Bacteriophages are viruses that specifically infect a bacterial host. They play a great role in the modern biotechnology and antibiotic-resistant microbe era. Since the discovery of phages, their application as a control agent has faced challenges that made antibiotics a better fit for combating pathogenic bacteria. Recently, with the novel sequencing technologies providing new insight into the nature of bacteriophages, their application has a second chance to be used. However, novel challenges need to be addressed to provide proper strategies for their practical application. This review focuses on addressing these challenges by initially introducing the nature of bacteriophages and describing the phage-host-dependent strategies for phage application. We also describe the effect of the long-term application of phages in natural environments and other bacterial communities. Overall, this review gathered crucial information for the future application of phages. We predict the use of phages will not be the only control strategy against pathogenic bacteria. Therefore, more studies must be done for low-risk control methods against antimicrobial-resistant bacteria.

Sign in / Sign up

Export Citation Format

Share Document