scholarly journals Bacterial morphotypes as biodiversity indicators in a scarcely explored ecosystem in Santiago de Cali, Colombia

Author(s):  
Laura Vergara-López ◽  
Alejandro Díaz-Ortíz ◽  
Doris Rosero-García

Abstract Bacteria are considered as the most diverse microorganisms on earth. However, bacterial diversity is unknown in some ecosystems, such as leachate treatment plants. In the present study, we characterized culturable bacteria isolated from six lagoons and one pure leachate to assess whether bacterial morphotypes can be used as biodiversity indicators. The sampling site was a leachate treatment plant located in Santiago de Cali. Overall, 311 bacterial morphotypes were collected from six lagoons, and a pure landfill leachate, and were identified using colony characters. Four biodiversity indexes related to species richness and abundance as well as to the estimation of the number of species present were calculated. The results show a great diversity of bacterial morphotypes in all samples. Further, the highest estimates of biodiversity were observed at lagoon 6 physicochemically treated, and the lowest estimates were observed at lagoons 5 and 8. The leachate generated in the sampled lagoons, which were composed of different elements, allows for the colonization of diverse bacteria. Moreover, these bacteria were resistant to the treatment plant processes, thereby explaining the highest diversity observed at the lagoon 6. Our results showed that bacterial morphotypes can be used as biodiversity indicators in ecosystems.

2020 ◽  
Vol 37 (10) ◽  
pp. 702-714
Author(s):  
Zubeda Ukundimana ◽  
Mehmet Kobya ◽  
Philip Isaac Omwene ◽  
Erhan Gengec ◽  
Orhan Taner Can

2006 ◽  
Vol 6 (6) ◽  
pp. 147-154 ◽  
Author(s):  
K.J. An ◽  
J.W. Tan ◽  
L. Meng

An advanced nitrogen removal pilot study was performed in China's Xia Ping Landfill Leachate Treatment Plant to undertake shortcut nitrification and denitrification with the Membrane Bio-reactor (MBR) process. It was found that the MBR process used 25% less of the oxygen and 40% less of the external carbon sources, compared to the conventional nitrification and denitrification process. The key feature of the MBR process is that it provides an environment more favorable for ammonia oxidation bacterium (AOB) than for nitrite oxidation bacterium (NOB) through controlling loading, pH, temperature, dissolved oxygen concentration (DO), and NH3 inhibition. Optimum operating condition was examined through continuous running of the pilot MBR, and it was found that a minimum HRT of 4.3 days and maximum ammonia loading of 0.6 kg N- NH4+ m3.d with pH 7–8.5, temperature 25–30 °C, and DO at 2 mg/L is favorable to AOB. Kinetic study was conducted to identify the characteristic of the microorganisms in the system. Measured Ks and μA,max of MBR sludge was 19.65 mg NH4-N/L (Temperature 25 °C, pH 8.5) and 0.26 d−1, respectively.


2017 ◽  
Vol 24 (11) ◽  
pp. 10364-10372 ◽  
Author(s):  
Gulizar Kurtoglu Akkaya ◽  
Elif Sekman ◽  
Selin Top ◽  
Ece Sagir ◽  
Mehmet Sinan Bilgili ◽  
...  

Chemosphere ◽  
2020 ◽  
Vol 242 ◽  
pp. 125207 ◽  
Author(s):  
Jia-Ying Wang ◽  
Xin-Li An ◽  
Fu-Yi Huang ◽  
Jian-Qiang Su

2021 ◽  
Vol Volume 53 (Special Issue A) ◽  
pp. 27-34
Author(s):  
M. Belouhova ◽  
N. Dinova ◽  
I. Yotinov ◽  
S. Lincheva ◽  
I. Schneider ◽  
...  

The landfill leachate is heavily polluted wastewater produced in the landfills. The management of the purification of the leachate is especially challenging and that is why new approaches and indicators are needed. The quantity, localization, interaction, clustering of the key microbial groups, responsible for the critical transformation processes can be used as indication leading to better performance of the technology. This study is focused on two bacterial groups (Anammox and Azoarcus-Thauera cluster) which have potential to serve as indicators for the landfill leachate treatment. Their quantity and activity were studied by FISH during lab-scale treatment of leachate from the Municipal Enterprise for Waste Treatment (MEWT), Sofia, Bulgaria. Two activated sludges (AS) were used – one from the MEWT and another form the WWTP (wastewater treatment plant) of Sofia. The obtained results showed that 74% of the COD was eliminated when leachate was diluted 50 and 25 times and 31% - when undiluted leachate was used. At the end of the process (21 day) the Azoarcus-Thauera group formed large aggregations in the AS from MEWT. They were 17.50% of the bacteria there while in the AS from the WWTP of Sofia they represented only 2.61%. The quantity of the anammox bacteria remained almost unchanged during the process and was 10.75% of the community from MEWT which eliminated 98 mg/L more ammonium ions at the end of the process and 6% from the community from the WWTP of Sofia. The two studied groups gave more complex information about the processes in the AS related to the elimination of the nitrogen and carbon containing pollutants. They could be used for better management of the biological processes during landfill leachate treatment. Key words: landfill leachate; anammox, Azoarcus-Thauera; activated sludge; fluorescence in-situ hybridization


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