FISH investigation of the bacterial groups anammox and Azoarcus-Thauera at treatment of landfill leachate

The landfill leachate is heavily polluted wastewater produced in the landfills. The management of the purification of the leachate is especially challenging and that is why new approaches and indicators are needed. The quantity, localization, interaction, clustering of the key microbial groups, responsible for the critical transformation processes can be used as indication leading to better performance of the technology. This study is focused on two bacterial groups (Anammox and Azoarcus-Thauera cluster) which have potential to serve as indicators for the landfill leachate treatment. Their quantity and activity were studied by FISH during lab-scale treatment of leachate from the Municipal Enterprise for Waste Treatment (MEWT), Sofia, Bulgaria. Two activated sludges (AS) were used – one from the MEWT and another form the WWTP (wastewater treatment plant) of Sofia. The obtained results showed that 74% of the COD was eliminated when leachate was diluted 50 and 25 times and 31% - when undiluted leachate was used. At the end of the process (21 day) the Azoarcus-Thauera group formed large aggregations in the AS from MEWT. They were 17.50% of the bacteria there while in the AS from the WWTP of Sofia they represented only 2.61%. The quantity of the anammox bacteria remained almost unchanged during the process and was 10.75% of the community from MEWT which eliminated 98 mg/L more ammonium ions at the end of the process and 6% from the community from the WWTP of Sofia. The two studied groups gave more complex information about the processes in the AS related to the elimination of the nitrogen and carbon containing pollutants. They could be used for better management of the biological processes during landfill leachate treatment. Key words: landfill leachate; anammox, Azoarcus-Thauera; activated sludge; fluorescence in-situ hybridization

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Anaerobic Digestion Process for the Treatment of Sisdole Landfill Leachate in Nepal

Journal of Southwest Jiaotong University ◽

10.35741/issn.0258-2724.55.4.35 ◽

2020 ◽

Vol 55 (4) ◽

Author(s):

Bikash Adhikari ◽

Shilpa Koirala

Keyword(s):

Anaerobic Digestion ◽

Removal Efficiency ◽

Landfill Leachate ◽

Waste Treatment ◽

Batch Reactor ◽

Batch Reactors ◽

Leachate Treatment ◽

Anaerobic Digestion Process ◽

Digestion Process ◽

Solids Concentration

Along with the population, organic waste has been rising significantly in recent years. The resulting uncontrollable waste loads and conventional methods of waste treatment have begun to cause chaos at the landfill sites. This study evaluates the performance of an anaerobic digestion process using batch reactors for the treatment of landfill leachate collected from the Sisdole landfill site in Nuwakot, Nepal. A lab-scale anaerobic batch reactor was set up in Kathmandu University, Nepal. Using an anaerobic digestion process, COD values of the leachate decreased from 2230 mg/l to 1125 mg/l (removal efficiency of ~50%), whereas total solids concentration decreased from 1925 to 925 mg/L under a retention time of 10 days. In addition, Monod’s model was established to design an Anaerobic Sequential Batch Reactor to achieve better performance, resulting in 85% removal efficiency for the leachate treatment. Overall, this study analyzed the anaerobic digestion process on the landfill leachate of Sisdole, and modeled the process to identify the conditions required for increasing the efficiency of treatment of Sisdole landfill leachate.

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Anodic Oxidation of Effluents from Stages of MBR-UF Municipal Landfill Leachate Treatment Plant

Environmental Engineering Science ◽

10.1089/ees.2020.0003 ◽

2020 ◽

Vol 37 (10) ◽

pp. 702-714

Author(s):

Zubeda Ukundimana ◽

Mehmet Kobya ◽

Philip Isaac Omwene ◽

Erhan Gengec ◽

Orhan Taner Can

Keyword(s):

Anodic Oxidation ◽

Landfill Leachate ◽

Treatment Plant ◽

Leachate Treatment ◽

Municipal Landfill

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Taxonomic and functional variations in the microbial community during the upgrade process of a full-scale landfill leachate treatment plant — from conventional to partial nitrification-denitrification

Frontiers of Environmental Science & Engineering ◽

10.1007/s11783-020-1272-7 ◽

2020 ◽

Vol 14 (6) ◽

Author(s):

Binbin Sheng ◽

Depeng Wang ◽

Xianrong Liu ◽

Guangxing Yang ◽

Wu Zeng ◽

...

Keyword(s):

Microbial Community ◽

Landfill Leachate ◽

Treatment Plant ◽

Full Scale ◽

Partial Nitrification ◽

Leachate Treatment

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In situ growth of anammox bacteria in subseafloor sediments

10.1101/729350 ◽

2019 ◽

Cited By ~ 1

Author(s):

Rui Zhao ◽

José M. Mogollón ◽

Sophie S. Abby ◽

Christa Schleper ◽

Jennifer F. Biddle ◽

...

Keyword(s):

Environmental Stressors ◽

Anammox Bacteria ◽

Deep Biosphere ◽

Active Growth ◽

Subsurface Sediments ◽

Cell Energy ◽

Energy Limitation ◽

Microbial Groups ◽

Favorable Conditions

The deep biosphere buried in marine sediments was estimated to host an equal number of microbes as found in the above oceans 1. It has been debated if these cells are alive and active 2, and their per cell energy availability does not seem to allow for net population growth 3. Here, we report the growth of anammox bacteria in ∼80,000 year old subsurface sediments indicated by their four orders of magnitude abundance increase in the nitrate-ammonia transition zone (NATZ). Their growth coincides with a local increase in anammox power supply. The genome of the dominant anammox bacterium from the NATZ was reconstructed and showed an increased index of replication confirming in situ active growth. The genome belongs to a new Scalindua species so far exclusively found in marine environments, which has the genetic capacity of urea and cyanate utilization and is enriched in genes allowing it to cope with external environmental stressors, such as energy limitation. Our results suggest that specific microbial groups are not only able to survive over geological timescales, but also thrive in the deep subsurface when encountering favorable conditions.

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Pilot study for the potential application of a shortcut nitrification and denitrification process in landfill leachate treatment with MBR

Water Science & Technology Water Supply ◽

10.2166/ws.2006.958 ◽

2006 ◽

Vol 6 (6) ◽

pp. 147-154 ◽

Cited By ~ 9

Author(s):

K.J. An ◽

J.W. Tan ◽

L. Meng

Keyword(s):

Pilot Study ◽

Landfill Leachate ◽

Ammonia Oxidation ◽

Carbon Sources ◽

Treatment Plant ◽

Optimum Operating ◽

Leachate Treatment ◽

Optimum Operating Condition ◽

Nitrification And Denitrification ◽

Denitrification Process

An advanced nitrogen removal pilot study was performed in China's Xia Ping Landfill Leachate Treatment Plant to undertake shortcut nitrification and denitrification with the Membrane Bio-reactor (MBR) process. It was found that the MBR process used 25% less of the oxygen and 40% less of the external carbon sources, compared to the conventional nitrification and denitrification process. The key feature of the MBR process is that it provides an environment more favorable for ammonia oxidation bacterium (AOB) than for nitrite oxidation bacterium (NOB) through controlling loading, pH, temperature, dissolved oxygen concentration (DO), and NH3 inhibition. Optimum operating condition was examined through continuous running of the pilot MBR, and it was found that a minimum HRT of 4.3 days and maximum ammonia loading of 0.6 kg N- NH4+ m3.d with pH 7–8.5, temperature 25–30 °C, and DO at 2 mg/L is favorable to AOB. Kinetic study was conducted to identify the characteristic of the microorganisms in the system. Measured Ks and μA,max of MBR sludge was 19.65 mg NH4-N/L (Temperature 25 °C, pH 8.5) and 0.26 d−1, respectively.

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