scholarly journals Prognostic Value of Heterotypic Neutrophil-in-Tumor Structure in Tongue Squamous Cell Carcinoma Patients

2020 ◽  
Author(s):  
Jie Fan ◽  
Yang Yang ◽  
Qigen Fang ◽  
Meng Cui ◽  
Wei Du ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Prognostic Value ◽  
Lymphovascular Invasion ◽  
Cox Model ◽  
Tumor Grade ◽  
Tumor Stage ◽  
Tongue Squamous Cell Carcinoma ◽  
Disease Stage

Abstract Objective: To analyze the prognostic value of frequency of heterotypic neutrophil-in-tumor structure(FNiT) in patients with tongue squamous cell carcinoma(TSCC).Methods: In vitro, we cocultured TSCC cell line-CAL33 with neutrophils to form heterotypic neutrophil-in-tumor structures, which were then subject to fluorescence staining. Clinically, 197 patients were enrolled. Information including age, sex, FNiT, ECOG PS(Performance Status), FH (family history) of cancer, complications, and pathological characteristics such as tumor stage, node stage, metastasis, disease stage, lymphovascular invasion, perineural invasion, tumor grade, and follow-up results was extracted and analyzed.Results: Fluorescent staining results of typical heterotypic neutrophil-in-tumor structure showed that well-differentiated CAL-33-2 had stronger ability to internalize more neutrophils than poorly-differentiated CAL-33-1 did, the latter often internalizing only one neutrophil. The mean FNiT was 4.8‰,with a range from 2.1‰ to 8.9‰. The FNiT was significantly associated with tumor stage, disease stage and tumor grade. A total of 119 patients died of the disease, and the 5-year disease-specific survival(DSS) rate was 36%. The median survival time was 52.6 months. In patients with an FNiT<4.8‰, the 5-year DSS rate was 40%; in patients with an FNiT>=4.8‰, the 5-year DSS was 28%, and the difference was significant(P=0.001). Cox model analysis showed that FNiT along with disease stage, lymphovascular invasion and tumor grade was an independent prognostic factor for DSS.Conclusion: The FNiT as a novel predictor is positively correlated with adverse prognosis of patients with TSCC.

Immunohistochemical Expression of TGF-β3 in Oral Squamous Cell Carcinoma

2019 ◽  
Vol 10 (01) ◽  
Author(s):  
Asmaa Ali Hussein
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Transforming Growth Factor ◽  
Tumor Grade ◽  
Tumor Stage ◽  
High Rate ◽  
Female Ratio ◽  
Positive Expression

Squamous cell carcinoma characterized by poor prognosis due to aggressive tumor growth and dissemination high rate of tumor cell . age ranged of patient case included in the study 40-62 years and mean age 55±99. The sex distribution male/female ratio 1:1. Male case 15 and female 15 of the present study The results of clinical forums showed in the current study was endophytic 10(33.3%) in the same time Exophytic were presented in 20 cases (76.7%). Regarding distribution of the tumors site, the preponderance of them 19 cases 73.3% were located alveolar mucosa, followed by in the tongue 11 cases(36.7%) Tumor stage was analyzed and recorded in Oral squamous cell carcinoma included cases, the preponderance of them were Stage II 11 cases 36.7% followed by stage III 10 cases 33.3% , 9 cases 30.0% were stage I. While Concerning tumor grade, majority of them 15 cases 50% had grade II moderately differentiated SCC, while 11 cases 36.7% had grade III poorly differentiated SCC and 4 cases 13.3% had grade I well differentiated SCC Positive TGF-β3 immunostaining was detected as cell with staining brown color, all tissues sections included show Positive expression based on IHC teqnique. Positive Transforming Growth Factor TGF-β3 Immuno staining was found in all case results and display that 4 samples with percentage 13.3% expressed strong positive 87.67 ± 1.45 expression , 11cases 36.7% showed 51.33 ±0.88 positive expression moderate at the same time 15 samples 50.0% showed positive weak expression.

scholarly journals Prognostic value of MRI-measured tumor thickness in patients with tongue squamous cell carcinoma

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Ki-Sun Park ◽  
Yangsean Choi ◽  
Jiwoong Kim ◽  
Kook-Jin Ahn ◽  
Bum-soo Kim ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Prognostic Value ◽  
Proportional Hazards ◽  
Correlation Coefficients ◽  
Cox Proportional Hazards ◽  
Tumor Thickness ◽  
Tongue Squamous Cell Carcinoma ◽  
Depth Of Invasion

AbstractThis study aimed to assess the prognostic value of MRI-measured tumor thickness (MRI-TT) in patients with tongue squamous cell carcinoma (SCC). This single-center retrospective cohort study included 133 pathologically confirmed tongue SCC patients between January 2009 and October 2019. MRI measurements of tongue SCC were based on axial and coronal T2-weighted (T2WI) and contrast-enhanced T1-weighted (CE-T1WI) images. Two radiologists independently measured MRI-TT. Intraclass correlation coefficients (ICC) were calculated for inter-rater agreements. Spearman’s rank correlation between MRI-TT and pathologic depth of invasion (pDOI) was assessed. Cox proportional hazards analyses on recurrence-free (RFS) and overall survival (OS) were performed for MRI-TT and pDOI. Kaplan–Meier survival curves were plotted with log-rank tests. The intra- and inter-rater agreements of MRI-TT were excellent (ICC: 0.829–0.897, all P < 0.001). The correlation between MRI-TT and pDOI was good (Spearman’s correlation coefficients: 0.72–0.76, P < 0.001). MRI-TT were significantly greater than pDOI in all axial and coronal T2WI and CE-T1WI (P < 0.001). In multivariate Cox proportional hazards analysis, MRI-TT measured on axial CE-T1WI yielded a significant prognostic value for OS (hazards ratio 2.77; P = 0.034). MRI-TT demonstrated excellent intra- and inter-rater agreements as well as high correlation with pDOI. MRI-TT may serve as a prognostic predictor in patients with tongue SCC.

To what degree is there interobserver variability among pathologists for T1 penile cancer?

2014 ◽  
Vol 32 (4_suppl) ◽  
pp. 381-381
Author(s):  
Adam Luchey ◽  
Patrick Espiritu ◽  
Gautum Agarwal ◽  
Chris Protzel ◽  
Jasreman Dhillon ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Lymph Node ◽  
Cell Carcinoma ◽  
Lymph Node Dissection ◽  
Squamous Cell ◽  
Penile Cancer ◽  
Tumor Grade ◽  
Tumor Stage ◽  
Node Dissection ◽  
European Model

381 Background: Molecular and genetic markers have yet to be developed to predict those patients that are at risk for lymph node metastasis. Currently, the grading of penile cancer plays a critical role in the determination of which patients receive an inguinal lymph node dissection (ILND) along with other treatment modalities. We sought out to determine the variance among genitourinary (GU) pathologists at a tertiary cancer center for penile cancer based on a European model. Methods: Nine patients that were diagnosed with stage pT1 primary penile squamous cell carcinoma were selected who underwent either a partial (8) or radical penectomy (1) from 10/2000 to 09/2009. All slides from each case were reviewed by each of the 3 reviewing pathologists, independently, who diagnosed the subtype of squamous cell carcinoma according to WHO criteria, assigned a grade, noted whether lymphovascular invasion was present or not and finally staged the tumor according to the AJCC Cancer Staging Manual, 7th edition. No access to the original, final pathological diagnosis was allowed. Interobserver variance between the 3 GU pathologists and each variable was calculated using Cohen’s kappa coefficient. Results: Complete agreement was reached in 3 cases for tumor grade and 4 cases for tumor stage out of 9. Overall, the 3 GU pathologists only displayed fair agreement at 30% for tumor grade (ê = 0.30, p = 0.018) and trended towards fair agreement at 24% (ê = 0.24, p = 0.077) and 25% (ê = 0.250, p = 0.097) for tumor stage and LVI respectively. Conclusions: The variance displayed herein demonstrates the difficulty in identifying individuals that would benefit from a diagnostic/therapeutic lymph node dissection based on pathological staging. This corroborates that of the European model and calls for novel methods to determine reproducible prognostic markers.

scholarly journals Impact of Oral Hygiene on Prognosis in Patients With Squamous Cell Carcinoma of the Lower Gingiva

2021 ◽  
Vol 8 ◽  
Author(s):  
Hui Zhao ◽  
Shengnan Zhang ◽  
Jinji Ma ◽  
Xiaodong Sun
Keyword(s):  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Oral Hygiene ◽  
Cox Model ◽  
Smoking Status ◽  
Disease Free Survival ◽  
Tumor Stage ◽  
Factors Affecting ◽  
Significant Difference

Objective: We aimed to analyze the significance of oral hygiene in patients with squamous cell carcinoma of the lower gingiva.Methods: Oral hygiene was assessed using a questionnaire by calculating the oral health (OH) score and the dental care (DC) score. The association of oral hygiene with clinical pathologic variables, disease free survival (DFS), and overall survival (OS) was analyzed.Results: Four out of 53 non-smokers and 19 out of 90 current or former smokers had an OH score of 6 (statistically significant difference, p = 0.036). Fifteen out of 63 patients with a T3/T4 tumor and 8 out of 80 patients with a T1/T2 tumor had an OH score of 6 (statistically significant difference, p = 0.026). Similar statistically significant findings were noted with respect to the DC scores, smoking status, and tumor stage. Among patients with an OH score of 0–5, the 5-year DFS and OS rates were 55 and 50%, respectively, and among patients with an OH score &gt;5, they were 46 and 43%, respectively (both differences statistically significant, p &lt; 0.05). Among patients with a DC score of 0–2, the 5-year DFS and OS rates were 69 and 51%, respectively and among patients with a DC score &gt;2, they were 50 and 47%, respectively (both differences statistically significant, p &lt; 0.05). Cox model confirmed OH and DC scores as independent factors affecting the DFS and OS.Conclusion: Poor oral hygiene was associated with decreased DFS and OS.

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