Methane Production Potential of Rumen Pretreated Lignocellulosic Wastes

Author(s):  
GOKCE KURT ◽  
Rumeysa Doluk ◽  
Hulya Civelek Yoruklu ◽  
Ahmet Demir ◽  
Bestami Ozkaya

Abstract Bioenergy production from lignocellulosic biomass is challenging due to its structure and a pretreatment is required before methane production. In this study, biological pretreatment by using rumen microorganisms was applied for different types of lignocellulosic wastes: wheat straw, cotton stalk, reeds and sunflower stalk. The reactors were pretreated for 2, 5, 10, 15 and 20 days. After the pretreatment stages and gas measurements were done, reactors were separated into two phases as lower solid phase and upper liquid phase. The reactors were installed for the methanation stage, gas measurements were made at regular intervals and graphs were drawn using the cumulative results. Modified Gompertz equation was used to estimate potential biogas production. According to the results, the reactor containing 5 days pretreated wheat straw became prominent among the other reactors in terms of biogas and methane production with 163 ml and 102 ml, respectively. It was followed by 20 days pretreated reeds with 104 ml biogas and 80 ml methane, 2 days pretreated sunflower stalk with 88 ml biogas and 52 ml methane, and 2 days pretreated cotton stalk with 87 ml biogas and 50 ml methane.

2017 ◽  
Vol 184 (1) ◽  
pp. 48-62 ◽  
Author(s):  
Ramkumar B. Nair ◽  
Maryam M. Kabir ◽  
Patrik R. Lennartsson ◽  
Mohammad J. Taherzadeh ◽  
Ilona Sárvári Horváth

AbstractIntegration of wheat straw for a biorefinery-based energy generation process by producing ethanol and biogas together with the production of high-protein fungal biomass (suitable for feed application) was the main focus of the present study. An edible ascomycete fungal strain Neurospora intermedia was used for the ethanol fermentation and subsequent biomass production from dilute phosphoric acid (0.7 to 1.2% w/v) pretreated wheat straw. At optimum pretreatment conditions, an ethanol yield of 84 to 90% of the theoretical maximum, based on glucan content of substrate straw, was observed from fungal fermentation post the enzymatic hydrolysis process. The biogas production from the pretreated straw slurry showed an improved methane yield potential up to 162% increase, as compared to that of the untreated straw. Additional biogas production, using the syrup, a waste stream obtained post the ethanol fermentation, resulted in a combined total energy output of 15.8 MJ/kg wheat straw. Moreover, using thin stillage (a waste stream from the first-generation wheat-based ethanol process) as a co-substrate to the biogas process resulted in an additional increase by about 14 to 27% in the total energy output as compared to using only wheat straw-based substrates.


2020 ◽  
Vol 13 (3) ◽  
pp. 833-844 ◽  
Author(s):  
Mirjam Victorin ◽  
Åsa Davidsson ◽  
Ola Wallberg

2022 ◽  
Vol 9 (1) ◽  
pp. 13
Author(s):  
Richard Arthur ◽  
Sebastian Antonczyk ◽  
Sandra Off ◽  
Paul A. Scherer

Lignocellulosic residues, such as straw, are currently considered as candidates for biogas production. Therefore, straw fermentations were performed to quantitatively estimate methane yields and cell counts, as well as to qualitatively determine the microbiome. Six fully automated, continuously stirred biogas reactors were used: three mesophilic (41 °C) and three thermophilic (58 °C). They were fed every 8 h with milled wheat straw suspension in a defined, buffered salt solution, called ‘synthetic manure’. Total reflection X-ray fluorescence spectrometry analyses showed nickel and tungsten deficiency in the straw suspension. Supplementation of nickel and subsequently tungsten, or with an increasing combined dosage of both elements, resulted in a final concentration of approximately 0.1 mg/L active, dissolved tungsten ions, which caused an increase of the specific methane production, up to 63% under mesophilic and 31% under thermophilic conditions. That is the same optimal range for pure cultures of methanogens or bacteria found in literature. A simultaneous decrease of volatile fatty acids occurred. The Ni/W effect occurred with all three organic loading rates, being 4.5, 7.5, and 9.0 g volatile solids per litre and day, with a concomitant hydraulic retention time of 18, 10, or 8 days, respectively. A maximum specific methane production of 0.254 m3 CH4, under standard temperature and pressure per kg volatile solids (almost 90% degradation), was obtained. After the final supplementation of tungsten, the cell counts of methanogens increased by 300%, while the total microbial cell counts increased by only 3–62%. The mesophilic methanogenic microflora was shifted from the acetotrophic Methanosaeta to the hydrogenotrophic Methanoculleus (85%) by tungsten, whereas the H2-CO2-converter, Methanothermobacter, always dominated in the thermophilic fermenters.


2016 ◽  
Vol 5 (2) ◽  
Author(s):  
Meena Krishania ◽  
Virendra K. Vijay ◽  
Ram Chandra

AbstractThis paper examines the performance results of configured semi-continuous mesophilic bioreactors for methane fermentation of pretreated wheat straw co-digested with cattle manure. The semi-continuous process was carried out in three different types of bioreactors, i.e. continuous stirred tank reactor (CSTR), fixed film reactor (FFR), and conventional floating drum reactor (CR), with an organic loading rate of 2.2 kg of volatile solids (VS)/day. The observed results revealed that the configuration of the FFR increased the methane production yield by 41.1%. However, the configuration of the CSTR had only a marginal effect on enhancement of methane production yield (yield increased only by 10.6%) compared to the CR. The VS removal for digesters did not vary much at the same organic loading rate. The study revealed that the pretreated wheat straw with the FFR had yield methane production of 0.342 m


2020 ◽  
Vol 6 (6) ◽  
pp. 1056-1063 ◽  
Author(s):  
Muhammad Jaffar Memon ◽  
Abdul Rehman Memon

The complex indigenous configuration of non-bio-labile wheat straw necessitates its pretreatment to optimize the breakdown of its structural components for its ultimate conversion into biogas by means of anaerobic digestion. In this research work, wheat straw was pretreated with potassium hydroxide (KOH) to facilitate its improved biodegradability. The pretreatment of wheat straw was also obvious in terms of its crystallinity resulting in the improved amorphous regions compared to the control wheat straw. The results showed that pretreated wheat straw digestion transpired into comparatively higher removal of TS (86%), VS (89%) and total lignin, cellulose and hemicellulose (22%) than that obtained with control wheat straw. Maximum biogas production accrued was 1550 mLN per day with optimized dosing of KOH compared to 967 mLN per day obtained with control wheat straw, implying that the cumulative biogas production was improved by 45% using pretreated wheat straw than that using control wheat straw. These results suggested that pretreated wheat straw digestion led to a significant improvement in the biogas yield.


2011 ◽  
Vol 347-353 ◽  
pp. 2996-3000 ◽  
Author(s):  
Jun Ling Niu ◽  
Lei Liu ◽  
Quan Guo Zhang

The study on the changes of composition and content reducing sugar of wheat straw after pretreated with composite microbial system for degrading cellulose was carried out, and the experimental study on the dry anaerobic fermentation of the pretreated wheat straw was followed. The results showed for pretreated wheat straw there is a significant increase for the amount of reducing sugar, the hemicellulose content decreased 49.43%, the cellulose content also decreased a little, but the lignin content had little change. For the dry anaerobic fermentation process, the reaction is started fast, the pH values declined faster in the prophase for the pretreated straw, the mixed biogas production and the methane content have been increased over that of non-pretreated wheat straw, and the gas was produced more steadily. The accumulated gas production had been to 4810mL, which was more than the non pretreated straw, before the maximum gas production was appeared.


2013 ◽  
Vol 164 (1) ◽  
pp. 50-58 ◽  
Author(s):  
Borbála Erdei ◽  
Balázs Frankó ◽  
Mats Galbe ◽  
Guido Zacchi

2007 ◽  
Vol 129 (11) ◽  
pp. 1415-1421 ◽  
Author(s):  
Joseph Borowsky ◽  
Timothy Wei

An experimental investigation of a two-phase pipe flow was undertaken to study kinematic and dynamic parameters of the fluid and solid phases. To accomplish this, a two-color digital particle image velocimetry and accelerometry (DPIV∕DPIA) methodology was used to measure velocity and acceleration fields of the fluid phase and solid phase simultaneously. The simultaneous, two-color DPIV∕DPIA measurements provided information on the changing characteristics of two-phase flow kinematic and dynamic quantities. Analysis of kinematic terms indicated that turbulence was suppressed due to the presence of the solid phase. Dynamic considerations focused on the second and third central moments of temporal acceleration for both phases. For the condition studied, the distribution across the tube of the second central moment of acceleration indicated a higher value for the solid phase than the fluid phase; both phases had increased values near the wall. The third central moment statistic of acceleration showed a variation between the two phases with the fluid phase having an oscillatory-type profile across the tube and the solid phase having a fairly flat profile. The differences in second and third central moment profiles between the two phases are attributed to the inertia of each particle type and its response to turbulence structures. Analysis of acceleration statistics provides another approach to characterize flow fields and gives some insight into the flow structures, even for steady flows.


Bioethanol ◽  
2016 ◽  
Vol 2 (1) ◽  
Author(s):  
María García-Torreiro ◽  
Miguel Álvarez Pallín ◽  
María López-Abelairas ◽  
Thelmo A. Lu-Chau ◽  
Juan M. Lema

AbstractBioconversion of lignocellulosic materials into ethanol requires an intermediate pretreatment step for conditioning biomass. Sugar yields from wheat straw were previously improved by the addition of a mild alkali pretreatment step before bioconversion by the white-rot fungus Irpex lacteus. In this work, an alternative alkaline treatment, which significantly reduces water consumption, was implemented and optimized. Sugar recovery increased 117% with respect to the previously developed alkaline wash process at optimal process conditions (30°C, 30 minutes and 35.7% (w/w) of NaOH). In order to further reduce operational costs, a system for alkali recycling was implemented. This resulted in the treatment of 150% more wheat straw using the same amount of NaOH. Finally, enzymatic hydrolysis was optimized and resulted in a reduction of enzyme dose of 33%.


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