scholarly journals Bioproduction of succinic acid from xylose by engineered Yarrowia lipolytica without pH control

2020 ◽  
Author(s):  
Ashish Prabhu ◽  
Rodrigo Ledesma- Amaro ◽  
Carol Sze Ki Lin ◽  
Frederic Coulon ◽  
Vijay kumar Thakur ◽  
...  
Keyword(s):  
Cell Growth ◽  
Carbon Source ◽  
Succinic Acid ◽  
Yarrowia Lipolytica ◽  
Recombinant Strain ◽  
Sole Carbon Source ◽  
Substrate Inhibition ◽  
Biomass Concentration ◽  
Value Added ◽  
Green Economy

Abstract Background Xylose is a most prevalent sugar available in hemicellulose fraction of lignocellulosic biomass (LCB) and of great interest for the green economy. Unfortunately, most of the cell factories cannot inherently metabolize xylose as sole carbon source. Yarrowia lipolytica is a non-conventional yeast to produce industrially important metabolites, and it is able to metabolize a large variety of substrates including both hydrophilic and hydrophobic carbon sources. However, Y. lipolytica lacks effective metabolic pathway for xylose uptake and only scarce information is available on utilization of xylose. For the economically feasible of LCB-based biorefineries, effective utilization of both pentose and hexose sugars is obligatory. Results In the present study, succinic acid (SA) production from xylose by Y. lipolytica was examined. To this end, Y. lipolytica PSA02004 strain was engineered by overexpressing pentose pathway cassette comprising of xylose reductase ( XR ), xylitol dehydrogenase ( XDH ) and xylulose kinase ( XK ) gene. The recombinant strain exhibited a robust growth on xylose as sole carbon source and accumulated SA (3.8 g/L) with a yield of 0.19 g/g in shake flask studies. Substrate inhibition studies revealed a marked negative impact on cell growth and product formation above 60 g/L xylose concentration. The modelling based on inhibition kinetics revealed that Aiba model showed better fit with experimental data, which resulted the correlation coefficient (R 2 ) of 0.82 and inhibition constant (K I ) 88.9 g/L. The batch cultivation of recombinant strain in bioreactor resulted in a maximum biomass concentration of 7.3 g/L and SA titer of 11.2 g/L with the yield of 0.18 g/g. Similar results in term of cell growth and SA production were obtained with xylose-rich hydrolysate derived from sugarcane bagasse. The fed-batch fermentation yielded biomass concentration of 11.8 g/L (OD 600 : 56.1) and SA titer of 22.3 g/L with a gradual decrease in pH below 4.0. Acetic acid was obtained as a main byproduct in all the fermentations. Conclusion The recombinant strain displayed potential bioconversion of xylose to succinic acid. Further this study provided a new insight on conversion of LCB into value-added products. To the best of our knowledge, this is the first study on SA production by Y. lipolytica using xylose as a sole carbon source.

scholarly journals Bioproduction of succinic acid from xylose by engineered Yarrowia lipolytica without pH control

2020 ◽  
Author(s):  
Ashish Prabhu ◽  
Rodrigo Ledesma- Amaro ◽  
Carol Sze Ki Lin ◽  
Frederic Coulon ◽  
Vijay kumar Thakur ◽  
...  
Keyword(s):  
Cell Growth ◽  
Carbon Source ◽  
Succinic Acid ◽  
Lignocellulosic Biomass ◽  
Yarrowia Lipolytica ◽  
Recombinant Strain ◽  
Sole Carbon Source ◽  
Biomass Concentration ◽  
Value Added ◽  
Green Economy

Abstract Background : Xylose is a most prevalent sugar available in hemicellulose fraction of lignocellulosic biomass (LCB) and of great interest for the green economy. Unfortunately, most of the cell factories cannot inherently metabolize xylose as sole carbon source. Yarrowia lipolytica is a non-conventional yeast to produce industrially important metabolites. The yeast is able to metabolize a large variety of substrates including both hydrophilic and hydrophobic carbon sources. However, Y. lipolytica lacks effective metabolic pathway for xylose uptake and only scarce information is available on utilization of xylose. For the economically feasibility of LCB-based biorefineries, effective utilization of both pentose and hexose sugars is obligatory. Results : In the present study, succinic acid (SA) production from xylose by Y. lipolytica was examined. To this end, Y. lipolytica PSA02004 strain was engineered by overexpressing pentose pathway cassette comprising of xylose reductase ( XR ), xylitol dehydrogenase ( XDH ) and xylulose kinase ( XK ) gene. The recombinant strain exhibited a robust growth on xylose as sole carbon source and produced substantial amount of SA. The inhibition of cell growth and SA formation was observed above 60 g/L xylose concentration. The batch cultivation of recombinant strain in bioreactor resulted in a maximum biomass concentration of 7.3 g/L and SA titer of 11.2 g/L with the yield of 0.19 g/g. Similar results in term of cell growth and SA production were obtained with xylose-rich hydrolysate derived from sugarcane bagasse. The fed-batch fermentation yielded biomass concentration of 11.8 g/L (OD 600 : 56.1) and SA titer of 22.3 g/L with a gradual decrease in pH below 4.0. Acetic acid was obtained as a main byproduct in all the fermentations. Conclusion : The recombinant strain displayed potential for bioconversion of xylose to SA. Further, this study provided a new insight on conversion of lignocellulosic biomass into value-added products. To the best of our knowledge, this is the first study on SA production by Y. lipolytica using xylose as a sole carbon source.

scholarly journals Biosynthesis of Polyhydroxyalkanoate Terpolymer from Methanol via the Reverse β-Oxidation Pathway in the Presence of Lanthanide

2022 ◽  
Vol 10 (1) ◽  
pp. 184
Author(s):  
Izumi Orita ◽  
Gento Unno ◽  
Risa Kato ◽  
Toshiaki Fukui
Keyword(s):  
Carbon Source ◽  
Sole Carbon Source ◽  
Ralstonia Eutropha ◽  
Negative Impact ◽  
Value Added ◽  
Pha Synthase ◽  
Growth Impairment ◽  
Oxidation Pathway ◽  
Methylotrophic Growth ◽  
Value Added Products

Methylorubrum extorquens AM1 is the attractive platform for the production of value-added products from methanol. We previously demonstrated that M. extorquens equipped with PHA synthase with broad substrate specificity synthesized polyhydroxyalkanoates (PHAs) composed of (R)-3-hydroxybutyrate and small fraction of (R)-3-hydroxyvalerate (3HV) and (R)-3-hydroxyhexanoate (3HHx) units on methanol. This study further engineered M. extorquens for biosynthesis of PHAs with higher 3HV and 3HHx composition focusing on the EMC pathway involved in C1 assimilation. The introduction of ethylmalonyl-CoA decarboxylase, catalyzing a backward reaction in the EMC pathway, aiming to increase intracellular propionyl/butyryl-CoA precursors did not affect PHA composition. Reverse b-oxidation pathway and subsequent (R)-specific hydration of 2-enoyl-CoA were then enhanced by heterologous expression of four genes derived from Ralstonia eutropha for the conversion of propionyl/butyryl-CoAs to the corresponding (R)-3-hydroxyacyl-CoA monomers. The resulting strains produced PHAs with higher 3HV and 3HHx compositions, while the methylotrophic growth was severely impaired. This growth impairment was interestingly restored by the addition of La3+ without a negative impact on PHA biosynthesis, suggesting the activation of the EMC pathway by La3+. The engineered M. extorquens synthesized PHA terpolymer composed of 5.4 mol% 3HV and 0.9% of 3HHx with 41% content from methanol as a sole carbon source in the presence of La3+.

scholarly journals The Overexpression of YALI0B07117g Results in EnhancedErythritol Synthesis from Glycerol by the Yeast Yarrowia lipolytica

Molecules ◽  
2021 ◽  
Vol 26 (24) ◽  
pp. 7549
Author(s):  
Mateusz Szczepańczyk ◽  
Dorota A. Rzechonek ◽  
Adam Dobrowolski ◽  
Aleksandra M. Mirończuk
Keyword(s):  
Carbon Source ◽  
Yarrowia Lipolytica ◽  
Sole Carbon Source ◽  
Consumption Rate ◽  
Batch Cultures ◽  
Cultivation Time ◽  
Erythrose Reductase ◽  
Yeast Yarrowia Lipolytica ◽  
Rate Of Consumption

The unconventional yeast Yarrowia lipolytica is used to produce erythritol from glycerol. In this study, the role of the erythrose reductase (ER) homolog YALI0B07117g in erythritol synthesis was analyzed. The deletion of the gene resulted in an increased production of mannitol (308%) and arabitol (204%) before the utilization of these polyols began. The strain overexpressing the YALI0B07117g gene was used to increase the erythritol yield from glycerol as a sole carbon source in batch cultures, resulting in a yield of 0.4 g/g. The specific consumption rate (qs) increased from 5.83 g/g/L for the WT strain to 8.49 g/g/L for the modified strain and the productivity of erythritol increased from 0.28 g/(L h) for the A101 strain to 0.41 g/(L h ) for the modified strain. The application of the research may prove positive for shortening the cultivation time due to the increased rate of consumption of the substrate combined with the increased parameters of erythritol synthesis.

Characterization of Liquid Pineapple Waste as Carbon Source for Production of Succinic Acid

2014 ◽  
Vol 69 (4) ◽  
Author(s):  
Norela Jusoh ◽  
Norasikin Othman ◽  
Ani Idris ◽  
Alina Nasruddin
Keyword(s):  
Carbon Source ◽  
Succinic Acid ◽  
Sugar Content ◽  
Liquid Waste ◽  
Value Added ◽  
Total Sugar Content ◽  
Pineapple Waste ◽  
Appropriate Treatment ◽  
Succinic Acid Production ◽  
Physical And Chemical

Pineapple cannery produces large amount of solid and liquid waste. The disposal of waste without an appropriate treatment can cause a great environmental pollution. Since pineapple waste contains some valuable components such as glucose, fructose and sucrose, the ability to convert this waste into higher value added product such as succinic acid would be advantageous. Therefore, in this study, liquid pineapple waste was characterized in order to investigate the possibility of succinic acid production via fermentation using liquid pineapple waste as a carbon source. The physical and chemical composition in the liquid pineapple waste such as cation, anion, pH, sugar content and soluble protein were determined. The dominant sugar in the liquid pineapple waste were glucose, fructose and sucrose and the total sugar content was more than 100 g/l. Result from the fermentation process proved that liquid pineapple waste can successfully produce succinic acid with almost the same amount as using glucose as carbon source, with the concentration of 6.26 g/l.

Gordonia (nocardia) amarae foaming due to biosurfactant production

2002 ◽  
Vol 46 (1-2) ◽  
pp. 519-524 ◽  
Author(s):  
K.R. Pagilla ◽  
A. Sood ◽  
H. Kim
Keyword(s):  
Surface Tension ◽  
Carbon Source ◽  
Stationary Phase ◽  
Activated Sludge ◽  
Sole Carbon Source ◽  
Wastewater Treatment Plants ◽  
Carbon Sources ◽  
Biomass Concentration ◽  
Culture Broth ◽  
Biosurfactant Production

Gordonia amarae, a filamentous actinomycete, commonly found in foaming activated sludge wastewater treatment plants was investigated for its biosurfactant production capability. Soluble acetate and sparingly soluble hexadecane were used as carbon sources for G. amarae growth and biosurfactant production in laboratory scale batch reactors. The lowest surface tension (critical micelle concentration, CMC) of the cell-free culture broth was 55 dynes/cm when 1,900 mg/L acetate was used as the sole carbon source. The lowest surface tension was less than 40 dynes/cm when either 1% (v/v) hexadecane or a mixture of 1% (v/v) hexadecane and 0.5% (w/v) acetate was used as the carbon source. The maximum biomass concentration (the stationary phase) was achieved after 4 days when acetate was used along with hexadecane, whereas it took about 8 days to achieve the stationary phase with hexadecane alone. The maximum biosurfactant production was 3 × CMC with hexadecane as the sole carbon source, and it was 5 × CMC with the mixture of hexadecane and acetate. Longer term growth studies (∼ 35 days of culture growth) indicated that G. amarae produces biosurfactant in order to solubilize hexadecane, and that adding acetate improves its biosurfactant production by providing readily degradable substrate for initial biomass growth. This research confirms that the foaming problems in activated sludge containing G. amarae in the activated sludge are due to the biosurfactant production by G. amarae when hydrophobic substrates such as hexadecane are present.

Production of succinic acid at low pH by a recombinant strain of the aerobic yeast Yarrowia lipolytica

2010 ◽  
Vol 107 (4) ◽  
pp. 673-682 ◽  
Author(s):  
Tigran V. Yuzbashev ◽  
Evgeniya Y. Yuzbasheva ◽  
Tatiana I. Sobolevskaya ◽  
Ivan A. Laptev ◽  
Tatiana V. Vybornaya ◽  
...  
Keyword(s):  
Succinic Acid ◽  
Yarrowia Lipolytica ◽  
Recombinant Strain ◽  
Low Ph ◽  
Yeast Yarrowia Lipolytica

scholarly journals An algal-bacterial symbiotic system of carbon fixation using formate as a carbon source

2021 ◽  
Author(s):  
Yurui Zheng ◽  
Jeffrey Czajka ◽  
Carly Daiek ◽  
Yinjie J. Tang ◽  
Liangliang Sun ◽  
...  
Keyword(s):  
Carbon Source ◽  
Carbon Fixation ◽  
Algal Growth ◽  
Biomass Concentration ◽  
Value Added ◽  
Microbial Community Analysis ◽  
Symbiotic System ◽  
Bacterial Assemblage ◽  
Mass Transfer Limitation ◽  
Light Irradiance

Algae are an attractive option for CO2 sequestration due to their natural ability to simultaneously fix CO2 and accumulate algal biomass for value-added products. However, the commercial implementation of such technology for efficient capture of CO2 from fossil-derived flue gases is not a reality yet due to several major challenges, such as low gas-liquid mass transport efficiency and relatively high light irradiance demand of algal growth. This study explored an algal-bacterial symbiotic system to utilize formate, a potential intermediate liquid compound of CO2, as carbon source to support microbial growth. The algal-bacterial assemblage, after an adaptive evolution using the formate medium, demonstrated a new route to assimilate CO2 without using high pH cultivations and promote biomass production under low light irradiance condition. The formate based culture system not only resolves CO2 mass transfer limitation, but also expels algae grazers in non-sterilized cultivation conditions. Continuous cultivation of the assemblage on formate led to a carbon capture efficiency of 90% with biomass concentration of 0.92 g/L and biomass productivity of 0.31 g/L/day, which is significantly better than the control cultivation on saturated CO2. In addition, isotope tracing and microbial community analysis offer new insights into formate metabolism and algal-bacterial symbiosis under light and carbon conditions. This study demonstrates a promising route of using electrochemical-derived formate to support algal biorefining.

scholarly journals Acetate Utilization for Yeast Cell Growth as Sole Carbon Source

1969 ◽  
Vol 33 (6) ◽  
pp. 977-978 ◽  
Author(s):  
Koichi OGATA ◽  
Hideo NISHIKAWA ◽  
Masahiro OHSUGI
Keyword(s):  
Cell Growth ◽  
Carbon Source ◽  
Yeast Cell ◽  
Sole Carbon Source ◽  
Acetate Utilization

scholarly journals Production of succinic acid from pineapple peel waste

2021 ◽  
Author(s):  
Panchalee Pathanibul ◽  
Choosit Hongkulsup
Keyword(s):  
Carbon Source ◽  
Succinic Acid ◽  
Low Cost ◽  
Value Added ◽  
Acid Synthesis ◽  
Nutritional Composition ◽  
Acid Yield ◽  
Sugar Release ◽  
Renewable Biomass ◽  
Pineapple Peel

Abstract Background: Succinic acid is a crucial platform chemical for production of various industrially significant compounds. For a sustainable and eco-friendly process, succinic acid synthesis has been shifted towards the fermentative route using renewable biomass substrates. Pineapple consumption and processing generate an immense amount of waste from its non-edible peel portion. As a carbon source, pineapple peel can be valorized for succinic acid bioproduction. Results: The hydrothermal pretreatment (121°C, 15 min) of pineapple peel waste resulted in the highest sugar release of 35.22 g/L (18 g/L glucose and 17 g/L fructose). The subsequent fermentation of pineapple peel hydrolysate was performed by a natural succinic acid producer, Actinobacillus succinogenes TISTR 1994. When the non-detoxified hydrolysate was used as a sole carbon source, 6.21 g/L of succinic acid was produced from 26.16 g/L of sugars. Additional supplementation of 9 g/L mixed nitrogen source enhanced the formation of succinic acid to 9.96 g/L from roughly the same amount of sugar. The current production conditions using mainly hydrolysate-based medium gave the succinic acid yield of 0.39 g/g sugar suggesting feasibilities for further improvement. Conclusion: Bio-based succinic acid production was attempted for the first time using the solid pineapple waste as a main starting material. Results demonstrated a proof of concept that the abundant pineapple peel waste can serve as a renewable substrate for a low-cost, value-added bioconversion to succinic acid. Optimization of nutritional composition in hydrolysate is necessary to enhance the yield of succinic acid in future studies.

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