scholarly journals The Prevailing O Serogroups Among the Serologically Differentiated Clinical Proteus Spp. Strains in Poland

2021 ◽  
Author(s):  
Dominika Drzewiecka ◽  
Agata Palusiak ◽  
Małgorzata Siwińska ◽  
Agnieszka Zabłotni
Keyword(s):  
Structural Feature ◽  
Western Blotting ◽  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serological Analysis ◽  
Rapid Changes ◽  
Unique Structural Feature ◽  
Serological Studies ◽  
Low Rate

Abstract In the years 2006-2011, 617 Proteus spp. strains isolated mostly from urine and wounds or other clinical sources from infected individuals were collected in Łódź, Poland. P. mirabilis species was dominating (86.9%), followed by P. genomospecies, P. vulgaris , and P. penneri . Ninety four per cent strains were recognized as S (smooth) forms. P. mirabilis exhibited the most intensive swarming growth. Serological studies (involving ELISA – enzyme-linked immunosorbent assay and Western blotting using native and adsorbed rabbit antisera) enabled classification of 80% S isolates into respective Proteus O serogroups among the 83 ones, described so far. The remaining strains seemed to be serologically unique. Detailed serological analysis confirmed that the recently described in Poland O78 serogroup is the most widespread. Also, 14 other serogroups have been found to predominate, being represented by ten or more strains. No unique structural feature of the prevalent O serotypes has been indicated. The observed big serological variety may suggest a low rate of Proteus spp. strains’ transmission between patients or rapid changes in their lipopolysaccharides. However, the prevalence of some O serogroups indicates that particular serotypes may be in some ways beneficial to the strains producing these kinds of O antigen.

scholarly journals The prevailing O serogroups among the serologically differentiated clinical Proteus spp. strains in central Poland

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Dominika Drzewiecka ◽  
Agata Palusiak ◽  
Małgorzata Siwińska ◽  
Agnieszka Zabłotni
Keyword(s):  
Structural Feature ◽  
Western Blotting ◽  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
O Antigen ◽  
Central Poland ◽  
Unique Structural Feature ◽  
Serological Studies

AbstractIn the years 2006–2011, 617 Proteus spp. strains isolated mostly from urine and wounds or other clinical sources were collected in Łódź, Poland, to determine the offensive O serotypes frequently occurring among patients. P. mirabilis exhibited the most intensive swarming growth and was dominating species (86.9%), followed by P. genomospecies, P. vulgaris, and P. penneri. Ninety four per cent strains were recognized as S (smooth) forms. Serological studies (involving ELISA—enzyme-linked immunosorbent assay and Western blotting using native and adsorbed rabbit antisera) enabled classification of 80% S isolates into respective Proteus O serogroups among the 83 ones, described so far. The remaining strains seemed to be serologically unique. Despite the observed big serological variety of Proteus spp. isolates, we found the O78 serogroup recently described in Poland as dominating and identified other widespread serotypes: O3, O6, O10, O11, O27, O28, and O30 reported earlier as predominating also in other countries; O77 and O79 detected lately in Poland; O16, O18, O20, and O50. No unique structural feature of the prevalent O serotypes has been indicated. However, the prevalence of some O serogroups indicates that particular serotypes may be in some ways beneficial to the strains producing these kinds of O antigen.

scholarly journals Serological Studies of Proteus penneri Strains Determining Qualification to Appropriate O-serogroup

2013 ◽  
Vol 62 (2) ◽  
pp. 211-216
Author(s):  
AGATA PALUSIAK ◽  
MAŁGORZATA SIWIŃSKA ◽  
ZYGMUNT SIDORCZYK
Keyword(s):  
Western Blot ◽  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serological Classification ◽  
Serological Studies ◽  
Reacting Systems ◽  
Proteus Penneri

Our Department of General Microbiology created a wide collection of P. penneri isolates and classified most of them into 19 different O-serogroups. This work describes the classification of 12 remaining P. penneri strains. The lipopolysaccharides extracted from P. penneri strains were tested in an enzyme-linked immunosorbent assay (ELISA) with selected O-antisera against Proteus sp. strains. Homologous and cross-reacting systems were checked in: passive immunohemolysis (PIH), inhibition of ELISA and PIH and Western blot procedure. These studies led to the qualification of tested P. penneri strains to five Proteus sp. O-serogroups, thus completing the serological classification of the whole collection.

scholarly journals Autoimmune antibodies correlate with immune checkpoint therapy-induced toxicities

2019 ◽  
Vol 116 (44) ◽  
pp. 22246-22251 ◽  
Author(s):  
Salahaldin A. Tahir ◽  
Jianjun Gao ◽  
Yuji Miura ◽  
Jorge Blando ◽  
Rebecca S. S. Tidwell ◽  
...  
Keyword(s):  
Adverse Events ◽  
Cancer Patients ◽  
Immunosorbent Assay ◽  
Immune Checkpoint ◽  
Additional Patient ◽  
Enzyme Linked Immunosorbent Assay ◽  
Plasma Samples ◽  
Serological Analysis ◽  
Autoimmune Antibodies ◽  
Expression Libraries

Immune checkpoint (IC) therapy provides substantial benefits to cancer patients but can also cause distinctive toxicities termed immune-related adverse events (irAEs). Biomarkers to predict toxicities will be necessary to improve management of patients receiving IC therapy. We relied on serological analysis of recombinant cDNA expression libraries to evaluate plasma samples from patients treated with IC therapy and identified autoantibodies, both in pretreatment and on-treatment samples prior to the development of irAEs, which correlate with the development of immune-related hypophysitis (anti-GNAL and anti-ITM2B autoantibodies) and pneumonitis (anti-CD74 autoantibody). We developed an enzyme-linked immunosorbent assay and tested additional patient samples to confirm our initial findings. Collectively, our data suggest that autoantibodies may correlate with irAEs related to IC therapy, and specific autoantibodies may be detected early for the management of irAEs.

A Comparison of an Enzyme Linked Immunosorbent Assay (ELISA) and Western Blotting for Detection of Peanut Mottle Virus and Peanut Stripe Virus1

1986 ◽  
Vol 13 (2) ◽  
pp. 64-67 ◽  
Author(s):  
J. L. Sherwood ◽  
H. A. Melouk
Keyword(s):  
Western Blotting ◽  
Immunosorbent Assay ◽  
Protein A ◽  
Mottle Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Coat Proteins ◽  
Western Blots ◽  
Peanut Stripe Virus ◽  
Dry Milk ◽  
The Difference

Abstract Western blotting was used to detect infections of peanut cv. Tamnut 74 with peanut mottle virus (PMV) and/or peanut stripe virus (PStV). Leaf samples were ground in electrophoresis sample buffer and heated for 5 min at 95 C prior to electrophoresis in 12% polyacrylamide gels. After electrophoresis, proteins were transferred to nitrocellulose sheets at 100V for 45 min. Western blots were performed by first blocking unbound sites on the nitrocellulose with 5% non-fat dry milk in Tris-buffered saline (TBS), pH 7.4 for 30 min, followed by incubation in a 1/200 dilution of PMV and/or PStV antiserum in TBS (the latter antiserum provided by J. W. Demski, U. of GA) for 45 min. This was followed by incubation in protein-A-peroxidase (2 μg/mL in TBS) for 45 min, followed by 4-chloro-1-napthol plus hydrogen peroxide in TBS. As little as 25 ng of either purified PMV or PStV was detected. This was similar to the limits of detection fo the double sandwich enzyme linked immunosorbent assay (ELISA). Because of the difference in migration of the coat proteins of PMV and PStV, both viruses may be detected in plants infected with PMV and PStV. This assay can be performed in approximately 6 h when mini-gels are used for the initial electrophoretic seperation and does not require the antiserum to be fractionated or bound to an enzyme as is the case with ELISA.

Serological Analysis by Enzyme-Linked Immunosorbent Assay Using Recombinant Antigen LipL32 for the Diagnosis of Swine Leptospirosis

2012 ◽  
Vol 66 (2) ◽  
pp. 106-109 ◽  
Author(s):  
Cláudia P. Hartleben ◽  
Fernanda M. A. Leal ◽  
Leonardo G. Monte ◽  
Daiane D. Hartwig ◽  
Fabiana K. Seixas ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Recombinant Antigen ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serological Analysis

Serological studies of Corynebacterium kutscheri and coryneform bacteria using an enzyme-linked immunosorbent assay (ELISA)

1995 ◽  
Vol 29 (3) ◽  
pp. 294-299 ◽  
Author(s):  
R. Boot ◽  
H. Thuis ◽  
R. Bakker ◽  
J. L. Veenema
Keyword(s):  
Immunosorbent Assay ◽  
Rattus Norvegicus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Limited Extent ◽  
Serological Relationship ◽  
Coryneform Bacteria ◽  
Homologous Antigen ◽  
Serological Studies

An enzyme-linked immunosorbent assay (ELISA) to measure Corynebacterium kutscheri antibodies in mice and rats was developed. Seven c. kutscheri isolates showed considerable serological relationship, but Japanese isolates differed from the British isolates. The ELISA appeared specific since c. kutscheri antigen did not react with antisera against 8 heterologous coryneform species. Antibodies to c. kutscheri were to a limited extent absorbed by autologous and homologous antigen, but not at all by the heterologous coryneform species. In naturally infected wild Rattus norvegicus and laboratory NA rats, the ELISA demonstrated high ODs to c. kutscheri.

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