Upregulation of S100 Calcium-Binding Protein A9 levels in the lungs of patients with idiopathic pulmonary fibrosis

2020 ◽  
Author(s):  
Jong-Uk Lee ◽  
Jong-Sook Park ◽  
Myung-Shin Kim ◽  
Jai-Seong Park ◽  
Eun-Suk Go ◽  
...  
Keyword(s):  
Idiopathic Pulmonary Fibrosis ◽  
Pulmonary Fibrosis ◽  
Calcium Binding ◽  
Binding Protein ◽  
Survival Rates ◽  
Surrogate Marker ◽  
Calcium Binding Protein ◽  
S100 Calcium Binding Protein ◽  
Neutrophil Percentage

Abstract Background: Neutrophilic inflammation is a predominant characteristic of idiopathic pulmonary fibrosis (IPF). S100 Calcium-Binding Protein A9 (S100A9) is a neutrophil-derived protein and is involved in the development of neutrophil-related chronic inflammatory disorders. However, the role of S100A9 in IPF has not been evaluated.Methods : S100A9 concentrations were measured by ELISA in the BAL fluid obtained from NCs (n = 33) and patients with IPF (n = 87), NSIP (n = 22), HP (n = 19), or sarcoidosis (n = 10).Results: The S100A9 levels in BALF were significantly higher in patients with IPF than in those with NC (0.4 [0.18–0.9] vs. 0 [0–0.5] ng/mL, p < 0.001), HP (0.19 [0.07–0.33] ng/mL, p = 0.043), or sarcoidosis (0.06 [0–0.11] ng/mL, p < 0.001) patients. A S100A9 level of 0.093 ng/mL had discriminating powers of 78.79% for specificity and 81.61% for sensitivity between IPF patients and NCs. S100A9 levels were also correlated with neutrophil numbers (r = 0.356, p = 0.0007) and S100A9 was expressed on neutrophils and macrophages in the BALF of IPF patients. Patients with S100A9 levels above 0.5535 ng/mL or a neutrophil percentage above 49.09% (n = 43) had significantly lower survival rates than those with S100A9 levels at or below 0.5535 ng/mL and a neutrophil percentage at or below 49.09% (n = 41) (HR, 9.28; p = 0.0004).Conclusion: S100A9 may participate in the development and progression of IPF. The levels of S100A9 in BALF may be a surrogate marker for diagnosing IPF and predicting its prognosis.

Clinical significance of serum S100 calcium‐binding protein A4 in idiopathic pulmonary fibrosis

Respirology ◽  
2019 ◽  
Vol 25 (7) ◽  
pp. 743-749 ◽  
Author(s):  
Norimichi Akiyama ◽  
Hironao Hozumi ◽  
Takuya Isayama ◽  
Jun Okada ◽  
Katsunori Sugiura ◽  
...  
Keyword(s):  
Idiopathic Pulmonary Fibrosis ◽  
Pulmonary Fibrosis ◽  
Clinical Significance ◽  
Calcium Binding ◽  
Binding Protein ◽  
Calcium Binding Protein ◽  
S100 Calcium Binding Protein

The S100 calcium-binding protein A4 level is elevated in the lungs of patients with idiopathic pulmonary fibrosis

2020 ◽  
Vol 171 ◽  
pp. 105945
Author(s):  
Jong-Uk Lee ◽  
Hun Soo Chang ◽  
Eun-Young Shim ◽  
Jai-Seong Park ◽  
Eun-Suk Koh ◽  
...  
Keyword(s):  
Idiopathic Pulmonary Fibrosis ◽  
Pulmonary Fibrosis ◽  
Calcium Binding ◽  
Binding Protein ◽  
Calcium Binding Protein ◽  
S100 Calcium Binding Protein

scholarly journals Downregulation of S100 Calcium Binding Protein A12 inhibits the growth of glioma cells

2020 ◽  
Author(s):  
Chunhe Lu ◽  
Jia Liu ◽  
Mingze Yao ◽  
Lun Li ◽  
Guangyu Li
Keyword(s):  
Cell Apoptosis ◽  
Calcium Binding ◽  
Binding Protein ◽  
S100 Protein ◽  
Glioma Cells ◽  
Calcium Binding Protein ◽  
Migration And Invasion ◽  
S100 Calcium Binding Protein ◽  
Tumorigenesis And Progression

Abstract Introduction: S100 Calcium Binding Protein A12 (S100A12) is a member of the S100 protein family and is widely expressed in neutrophil and low expressed in lymphocytes and monocyte. However, the role of S100A12 in glioma has not yet been identified. Methods: In the present study, we carried out immunohistochemical investigation of S100A12 in 81 glioma tissues to determine the expression of s100A12 in glioma cells, and evaluate the clinical significance of S100A12 in glioma patients. Futher we knockdown the S100A12 by ShRNA, and evaluated cell proliferation, cell migration and cell apoptosis by MTT,clony formation assay, transwell assay ,flow cytometry assa and westernblot. Results: We found that S100A12 was upregulated in tissues of glioma patients and the expression was correlated to WHO stage and tumor size. Further, we found that knockdown S100A12 inhibits the proliferation, migration and invasion of glioma cells through regulating cell apoptosis and EMT. Conclusions: These findings demonstrated a novel function for S100A12 in glioma progression and suggested that S100A12 may be served as a new marker in the tumorigenesis and progression of glioma.

scholarly journals Calcium-binding protein S100A4 confers mesenchymal progenitor cell fibrogenicity in idiopathic pulmonary fibrosis

2017 ◽  
Vol 127 (7) ◽  
pp. 2586-2597 ◽  
Author(s):  
Hong Xia ◽  
Adam Gilbertsen ◽  
Jeremy Herrera ◽  
Emilian Racila ◽  
Karen Smith ◽  
...  
Keyword(s):  
Idiopathic Pulmonary Fibrosis ◽  
Pulmonary Fibrosis ◽  
Calcium Binding ◽  
Progenitor Cell ◽  
Binding Protein ◽  
Calcium Binding Protein ◽  
Mesenchymal Progenitor Cell

scholarly journals Downregulation of S100 Calcium Binding Protein A12 inhibits the growth of glioma cells

2020 ◽  
Author(s):  
Chunhe Lu ◽  
Jia Liu ◽  
Mingze Yao ◽  
Lun Li ◽  
Guangyu Li
Keyword(s):  
Cell Apoptosis ◽  
Calcium Binding ◽  
Binding Protein ◽  
S100 Protein ◽  
Glioma Cells ◽  
Calcium Binding Protein ◽  
Migration And Invasion ◽  
Transwell Assay ◽  
S100 Calcium Binding Protein

Abstract Background: S100 Calcium Binding Protein A12 (S100A12) is a member of the S100 protein family and is widely expressed in neutrophil and low expressed in lymphocytes and monocyte. However, the role of S100A12 in glioma has not yet been identified. Methods: In the present study, we carried out immunohistochemical investigation of S100A12 in 81 glioma tissues to determine the expression of s100A12 in glioma cells, and evaluate the clinical significance of S100A12 in glioma patients. Futher we knockdown the S100A12 by ShRNA, and evaluated cell proliferation, cell migration and cell apoptosis by MTT, clony formation assay, transwell assay ,flow cytometry assa and western blot. Results: We found that S100A12 was upregulated in tissues of glioma patients and the expression was correlated to WHO stage and tumor size. Further, we found that knockdown S100A12 inhibits the proliferation, migration and invasion of glioma cells through regulating cell apoptosis and EMT. Background: S100 Calcium Binding Protein A12 (S100A12) is a member of the S100 protein family and is widely expressed in neutrophil and low expressed in lymphocytes and monocyte. However, the role of S100A12 in glioma has not yet been identified. Methods: In the present study, we carried out immunohistochemical investigation of S100A12 in 81 glioma tissues to determine the expression of s100A12 in glioma cells, and evaluate the clinical significance of S100A12 in glioma patients. Futher we knockdown the S100A12 by ShRNA, and evaluated cell proliferation, cell migration and cell apoptosis by MTT, clony formation assay, transwell assay ,flow cytometry assa and western blot. Results: We found that S100A12 was upregulated in tissues of glioma patients and the expression was correlated to WHO stage and tumor size. Further, we found that knockdown S100A12 inhibits the proliferation, migration and invasion of glioma cells through regulating cell apoptosis and EMT.

scholarly journals Downregulation of S100 Calcium Binding Protein A12 inhibits the growth of glioma cells

2019 ◽  
Author(s):  
Chunhe Lu ◽  
Jia Liu ◽  
Mingze Yao ◽  
Lun Li ◽  
Guangyu Li
Keyword(s):  
Cell Apoptosis ◽  
Calcium Binding ◽  
Binding Protein ◽  
S100 Protein ◽  
Glioma Cells ◽  
Calcium Binding Protein ◽  
Migration And Invasion ◽  
S100 Calcium Binding Protein ◽  
Tumorigenesis And Progression

Abstract Introduction: S100 Calcium Binding Protein A12 (S100A12) is a member of the S100 protein family and is widely expressed in neutrophil and low expressed in lymphocytes and monocyte. However, the role of S100A12 in glioma has not yet been identified. Methods: In the present study, we carried out immunohistochemical investigation of S100A12 in 81 glioma tissues to determine the expression of s100A12 in glioma cells, and evaluate the clinical significance of S100A12 in glioma patients. Futher we knockdown the S100A12 by ShRNA, and evaluated cell proliferation, cell migration and cell apoptosis by MTT,clony formation assay, transwell assay ,flow cytometry assa and westernblot. Results: We found that S100A12 was upregulated in tissues of glioma patients and the expression was correlated to WHO stage and tumor size. Further, we found that knockdown S100A12 inhibits the proliferation, migration and invasion of glioma cells through regulating cell apoptosis and EMT. Conclusions: These findings demonstrated a novel function for S100A12 in glioma progression and suggested that S100A12 may be served as a new marker in the tumorigenesis and progression of glioma.

scholarly journals Downregulation of S100 Calcium Binding Protein A12 inhibits the growth of glioma cells

2020 ◽  
Author(s):  
Chunhe Lu ◽  
Jia Liu ◽  
Mingze Yao ◽  
Lun Li ◽  
Guangyu Li
Keyword(s):  
Cell Apoptosis ◽  
Calcium Binding ◽  
Binding Protein ◽  
S100 Protein ◽  
Glioma Cells ◽  
Calcium Binding Protein ◽  
Migration And Invasion ◽  
S100 Calcium Binding Protein ◽  
Tumorigenesis And Progression

Abstract Introduction: S100 Calcium Binding Protein A12 (S100A12) is a member of the S100 protein family and is widely expressed in neutrophil and low expressed in lymphocytes and monocyte. However, the role of S100A12 in glioma has not yet been identified. Methods: In the present study, we carried out immunohistochemical investigation of S100A12 in 81 glioma tissues to determine the expression of s100A12 in glioma cells, and evaluate the clinical significance of S100A12 in glioma patients. Futher we knockdown the S100A12 by ShRNA, and evaluated cell proliferation, cell migration and cell apoptosis by MTT,clony formation assay, transwell assay ,flow cytometry assa and westernblot. Results: We found that S100A12 was upregulated in tissues of glioma patients and the expression was correlated to WHO stage and tumor size. Further, we found that knockdown S100A12 inhibits the proliferation, migration and invasion of glioma cells through regulating cell apoptosis and EMT. Conclusions: These findings demonstrated a novel function for S100A12 in glioma progression and suggested that S100A12 may be served as a new marker in the tumorigenesis and progression of glioma.

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