Genome sequencing survey and identification of SSR of Lycium ruthenicum
Abstract Background Lycium ruthenicum had high economic and ecological role in western China due to the high content of active substances and tolerance to drought and salinity stress. But its genomic information was lack, which seriously affected the next breeding and forestation. We surveyed the genomic size and developed SSRs of L. ruthenicum based on the next generation sequencing technology to lay a theoretical foundation for next genomic research in this study. Results Totally 451,721,828 bp raw data were generated, 4,596,439 scaffolds were obtained after assembly. The estimated genome size of L. ruthenicum was 3,249.33 Mb, the heterozygosity rate was 1.13%, and repeat rate was 73.13%. Totally 958,619 SSRs were identified. The average SSRs density were 163.95 SSRs/Mb, the dinucleotide repeat motif accounted for larger proportion in all motifs, the AT/AT, AC/GT and AG/CT are dominant repeat motifs in L. ruthenicum genome. Conclusion These results could lay a foundation for next genome sequencing. And SSR data could alarge the molecular resources for L. ruthenicum and relatives, such as genetic mapping, QTL and population genetic study.