Mucosa-associated Cultivable Aerobic Gut Bacterial Microbiota among Colorectal Cancer Patients Attending at the Referral Hospitals of Amhara Regional State, Ethiopia

Abstract Background: Colorectal cancer is one of the top ten cancer death in the world. Despite an increased prevalence of colorectal cancer has been documented from developing countries, reports on gut microbiota among colorectal cancer patients are none especially in Ethiopia. Therefore, the current study evaluated cultivable aerobic bacterial distributions among malignant tissue of colorectal cancer and its adjacent normal biopsies. Methods: Fifteen CRC patients who were undergoing colorectal cancer resection surgery during April 2017 to February 2018 at Felege Hiwot Referral and University of Gondar Teaching Hospitals were included. Biopsy specimens were taken from malignant and its adjacent normal tissues. Bacterial cultivation, quantification and characterization of saline washed biopsies were performed under aerobic and candle jar conditions. Differences in bacterial microbiota compositions between malignant and normal tissue biopsies were evaluated and analyzed using Microsoft excel 2010 and GraphPad Prism5 statistical software. Results: Fifteen CRC patients were participated with a mean age of 53.8 ± 10.8 years old and majorities (73.3%) of patients were in between the age groups of 40 and 60 years old. The mean ± SD bacterial microbiota of malignant biopsies (3.2x10 5 ± 1.6x10 5 CFU/ml) was significantly fewer than that of adjacent normal tissue biopsies (4.0x10 5 ± 2.2x10 5 CFU/ml). This dysbacteriosis is positively correlated with the occurrence of CRC (p=0.019). Proteobacteria (55.6%), Firmicutes (33.3%) and Fusobacteria (11.1%) were the most frequently isolated phyla from non-malignant biopsies while only Proteobacteria (58.8%) and Firmicutes (41.2%) were from malignant ones. Family level differences were observed among phyla (Firmicutes and Proteobacteria) isolated from the study participants. For instance, the relative abundance of family Bacillaceae from malignant (26%) was lower than the normal biopsies (39%). On other hand, family Enterobacteriaceae was twice more abundant in malignant tissues (45%) than in its matched normal tissues (23%). Furthermore, the family Enterococcaceae (14%) of family Firmicutes was solely isolated from malignant tissue biopsies. Conclusion: The overall microbial composition of normal and malignant tissues was considerably different among the study participants. Further culture independent analysis of mucosal microbiota will provide detail pictures of microbial composition differences and pathogenesis of CRC in Ethiopian settings.

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Mucosa-associated Cultivable Aerobic Gut Bacterial Microbiota among Colorectal Cancer Patients Attending at the Referral Hospitals of Amhara Regional State, Ethiopia

10.21203/rs.3.rs-33265/v2 ◽

2020 ◽

Author(s):

Yesuf Adem Siraj ◽

Melese Gebeyehu Byadgelegn ◽

Mensur Osman Yassin ◽

Yohannes Zenebe Chekol

Keyword(s):

Colorectal Cancer ◽

Cancer Patients ◽

Normal Tissue ◽

Age Groups ◽

Teaching Hospitals ◽

Microbial Composition ◽

Colorectal Cancer Resection ◽

Bacterial Microbiota ◽

Colorectal Cancer Patients ◽

Study Participants

Abstract Background: Colorectal cancer (CRC) is one of the top ten causes of cancer deaths in the world. Despite an increased prevalence of colorectal cancer has been documented from developing countries, there is no any report regarding gut microbiota among colorectal cancer patients in Ethiopia. Therefore, the current study evaluated cultivable aerobic gut bacterial distributions among malignant and its adjacent normal biopsies of CRC patients.Methods: CRC patients who were under colorectal cancer resection surgery during April 2017 to February 2018 at Felege Hiwot Referral and University of Gondar Teaching Hospitals enrolled in the study. Biopsy specimens were taken from malignant and its adjacent normal-appearing tissues. Bacterial cultivation, quantification and characterization of saline washed biopsies were performed under aerobic and candle jar conditions. Differences in bacterial microbiota compositions between malignant and normal tissue biopsies were evaluated and analyzed using Microsoft excel 2010 and GraphPad Prism5 statistical software.Results: Fifteen CRC patients were participated with a mean age of 53.8 + 10.8 years old and majorities (73.3%) of patients were in between the age groups of 40 and 60 years old. The mean + SD bacterial microbiota of malignant biopsies (3.2x105 + 1.6x105 CFU/ml) was significantly fewer than that of adjacent normal tissue biopsies (4.0x105 + 2.2x105 CFU/ml). This dysbacteriosis is positively correlated with the occurrence of CRC (p=0.019). Proteobacteria (55.6%), Firmicutes (33.3%) and Fusobacteria (11.1%) were the most frequently isolated phyla from non-malignant biopsies while only Proteobacteria (58.8%) and Firmicutes (41.2%) from malignant ones. Family level differences were observed among phyla (Firmicutes and Proteobacteria) isolated from the study participants. For instance, the relative abundance of family Bacillaceae from malignant (26%) was lower than the normal biopsies (39%). On other hand, family Enterobacteriaceae was twice more abundant in malignant tissues (45%) than in its matched normal tissues (23%). Furthermore, the family Enterococcaceae (14%) of phylum Firmicutes was solely isolated from malignant tissue biopsies. Conclusion: The overall microbial composition of normal and malignant tissues was considerably different among the study participants. Further studies with culture independent analysis of mucosal microbiota will provide detail pictures of microbial composition and pathogenesis of CRC in Ethiopian settings.

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Mucosa‐associated cultivable aerobic gut bacterial microbiota among colorectal cancer patients attending at the referral hospitals of Amhara Regional State, Ethiopia

Gut Pathogens ◽

10.1186/s13099-021-00415-7 ◽

2021 ◽

Vol 13 (1) ◽

Author(s):

Yesuf Adem Siraj ◽

Melesse Gebeyehu Biadgelign ◽

Mensur Osman Yassin ◽

Yohannes Zenebe Chekol

Keyword(s):

Colorectal Cancer ◽

Cancer Patients ◽

Normal Tissue ◽

Age Groups ◽

Teaching Hospitals ◽

Microbial Composition ◽

Colorectal Cancer Resection ◽

Bacterial Microbiota ◽

Colorectal Cancer Patients ◽

Study Participants

Abstract Background Colorectal cancer (CRC) is one of the top ten causes of cancer deaths in the world. Despite an increased prevalence of colorectal cancer has been documented from developing countries, there is no any report regarding gut microbiota among colorectal cancer patients in Ethiopia. Therefore, the current study evaluated cultivable aerobic gut bacterial distributions among malignant and its adjacent normal biopsies of CRC patients. Methods CRC patients who were under colorectal cancer resection surgery during April 2017 to February 2018 at Felege Hiwot Referral and University of Gondar Teaching Hospitals enrolled in the study. Biopsy specimens were taken from malignant and its adjacent normal-appearing tissues. Bacterial cultivation, quantification and characterization of saline washed biopsies were performed under aerobic and candle jar conditions. Differences in bacterial microbiota compositions between malignant and normal tissue biopsies were evaluated and analyzed using Microsoft excel 2010 and GraphPad Prism5 statistical software. Results Fifteen CRC patients were participated with a mean age of 53.8 ± 10.8 years old and majorities (73.3 %) of patients were in between the age groups of 40 and 60 years old. The mean ± SD bacterial microbiota of malignant biopsies (3.2 × 105 ± 1.6 × 105 CFU/ml) was significantly fewer than that of adjacent normal tissue biopsies (4.0 × 105 ± 2.2 × 105 CFU/ml). This dysbacteriosis is positively correlated with the occurrence of CRC (p = 0.019). Proteobacteria (55.6 %), Firmicutes (33.3 %) and Fusobacteria (11.1 %) were the most frequently isolated phyla from non-malignant biopsies while only Proteobacteria (58.8 %) and Firmicutes (41.2 %) were from malignant ones. Family level differences were observed among phyla (Firmicutes and Proteobacteria) isolated from the study participants. For instance, the relative abundance of family Bacillaceae from malignant (26 %) was lower than the normal biopsies (39 %). On other hand, family Enterobacteriaceae was twice more abundant in malignant tissues (45 %) than in its matched normal tissues (23 %). Furthermore, the family Enterococcaceae (14 %) of phylum Firmicutes was solely isolated from malignant tissue biopsies. Conclusions The overall microbial composition of normal and malignant tissues was considerably different among the study participants. Further culture independent analysis of mucosal microbiota will provide detail pictures of microbial composition differences and pathogenesis of CRC in Ethiopian settings.

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High KLF4 level in normal tissue predicts poor survival in colorectal cancer patients

World Journal of Surgical Oncology ◽

10.1186/1477-7819-12-232 ◽

2014 ◽

Vol 12 (1) ◽

pp. 232 ◽

Cited By ~ 22

Author(s):

Ha-young Lee ◽

Joong Ahn ◽

Sun Rha ◽

Hyun Chung ◽

Kyu Park ◽

...

Keyword(s):

Colorectal Cancer ◽

Cancer Patients ◽

Normal Tissue ◽

Poor Survival ◽

Colorectal Cancer Patients

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Expression of ADAM28 and IGFBP-3 Genes in Patients with Colorectal Cancer — A Preliminary Report

International Journal of Immunopathology and Pharmacology ◽

10.1177/039463201302600122 ◽

2013 ◽

Vol 26 (1) ◽

pp. 223-228 ◽

Cited By ~ 8

Author(s):

E. Nowakowska-Zajdel ◽

U. Mazurek ◽

J. Wierzgoń ◽

T. Kokot ◽

E. Fatyga ◽

...

Keyword(s):

Gene Expression ◽

Colorectal Cancer ◽

Cancer Patients ◽

Normal Tissue ◽

Oligonucleotide Microarray ◽

Control Group ◽

Tissue Specimens ◽

Colorectal Cancer Patients ◽

Microarray Technique ◽

Igfbp 3

Adamalisynes ( ADAMs) play an important role in inter-membrane interactions, cell adhesion and fusion processes and protein shedding from the cell surface. Many reports indicate that members of the ADAMs family are overexpressed in human cancer. The aim of the present study was to evaluate ADAM28 and Insulin Like Growth Factor Binding Protein-3 ( IGFBP-3)) gene expression in colorectal carcinoma tissues with regard to the overweight or obese status of the patients using an oligonucleotide microarray technique. Fresh tissue specimens were obtained from colorectal cancer patients during surgical treatment. Eighteen specimens from tumour and 18 normal tissue specimens from colorectal cancer patients at clinical stages III and IV were analysed. The examined patients were divided into two groups; those with BMI≥25 and those with normal BMI. The control group consisted of 18 specimens of non-neoplastic colon tissues, which were divided between overweight/obese and normal body weight patients. The gene transcriptional activity from the specimens was analysed using an oligonucleotide microarray technique. Microarrays and rinsing and marking solutions were prepared according to the procedure in the Gene Expression Analysis Technical Manual. The following conclusions were made: i) change of ADAM28 and IGFBP-3 genes expression are present in the normal tissue in overweight/obese patients with colorectal cancer only; ii) the observed molecular variability of ADAM28 and IGFBP-3 expression may be an initial process of cancer proliferation; iii) the histopathologically normal surgical margin in this group of patients was not equal to the molecular margin.

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The Differential DNA Hypermethylation Patterns of microRNA-137 and microRNA-342 Locus in Early Colorectal Lesions and Tumours

Biomolecules ◽

10.3390/biom9100519 ◽

2019 ◽

Vol 9 (10) ◽

pp. 519

Author(s):

Elham Kashani ◽

Mahrooyeh Hadizadeh ◽

Vahid Chaleshi ◽

Reza Mirfakhraie ◽

Chris Young ◽

...

Keyword(s):

Colorectal Cancer ◽

Cancer Patients ◽

Methylation Status ◽

Dna Hypermethylation ◽

Normal Colon Mucosa ◽

Normal Tissues ◽

Diagnosis And Prognosis ◽

Adjacent Mucosa ◽

Prediction And Prevention ◽

Colorectal Cancer Patients

Colorectal cancer (CRC) is the third most commonly diagnosed cancer worldwide, representing 13% of all cancers. The role of epigenetics in cancer diagnosis and prognosis is well established. MicroRNAs in particular influence numerous cancer associated processes including apoptosis, proliferation, differentiation, cell-cycle controls, migration/invasion and metabolism. MiRNAs-137 and 342 are exon- and intron-embedded, respectively, acting as tumour-suppressive microRNA via hypermethylation events. Levels of miRNAs 137 and 342 have been investigated here as potential prognostic markers for colorectal cancer patients. The methylation status of miRNA-137 and miRNA-342 was evaluated using methylation-specific (MSP) polymerase chain reaction (PCR) on freshly frozen tissue derived from 51 polyps, 8 tumours and 14 normal colon mucosa specimens. Methylation status of miRNA-137 and miRNA-342 was significantly higher in tumour lesions compared to normal adjacent mucosa. Surprisingly, the methylation frequency of miR-342 (76.3%) among colorectal cancer patients was significantly higher compared to miR-137 (18.6%). Furthermore, normal tissues, adjacent to the lesions (N-Cs), displayed no observable methylation for miRNA-137, whereas 27.2% of these N-Cs showed miRNA-342 hypermethylation. MiRNA-137 hypermethylation was significantly higher in male patients and miR-342 hypermethylation correlated with patient age. Methylation status of miRNA-137 and miRNA-342 has both diagnostic and prognostic value in CRC prediction and prevention.

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3089 Clinicopathologic and Prognostic Significance of LGR5, a Cancer Stem cell Marker in Peritoneal Metastasis of a Colorectal Origin

Journal of Clinical and Translational Science ◽

10.1017/cts.2019.234 ◽

2019 ◽

Vol 3 (s1) ◽

pp. 103-103

Author(s):

Chukwuemeka Ihemelandu

Keyword(s):

Colorectal Cancer ◽

Stem Cell ◽

Survival Analysis ◽

Cancer Patients ◽

Peritoneal Metastasis ◽

Prognostic Significance ◽

Stem Cell Marker ◽

Normal Tissues ◽

Lgr5 Expression ◽

Colorectal Cancer Patients

OBJECTIVES/SPECIFIC AIMS: Leucine-rich repeat-containing G-protein-coupled receptor 5 (LGR5) is expressed on Wnt/β-catenin-dependent adult stem cell populations of the colon. Cancer stem cells are hypothesized to be the driving force behind tumor progression and metastasis, making them attractive therapeutic targets. Our aim was to analyze the clinicopathologic and prognostic significance of LGR5 expression in a cohort of colorectal cancer patients with peritoneal metastasis. METHODS/STUDY POPULATION: A total of 49 Formalin-fixed paraffin-embedded (FFPE) tissue blocks of primary or metastatic tumors and their respective normal tissues were collected from the tissue bank for time period 2009-2015. LGR5 expression was assessed at the protein level through immunohistochemical (IHC) staining of tissue microarray (TMA) constructs consisting of pairs of tumor and normal colon tissue. The correlation between LGR5 expression and clinicopathologic parameters and prognosis was assessed by statistical analysis. RESULTS/ANTICIPATED RESULTS: Of the 49 patient sample, 30(61.22%) were female vs. 19 (38.78%) males. Age range at initial diagnosis ranged from 31.7 years to 84.4 years, with a median age of 61.29 years. Duration of follow-up ranged from 1 – 9 years with a median of 5 years.LGR5 expression was higher in colorectal cancer than in normal mucosa. In univariate survival analysis overexpression of LGR5 was significantly associated with improved survival (p=0.002).Of significance, LGR5 positivity was an independent prognostic marker for better prognosis in a multivariate survival analysis adjusting for prognostic variables age, stage, gender, tumor histology and grade (HR 2.67. 95% CI 1.01-7.00, P = 0.046). DISCUSSION/SIGNIFICANCE OF IMPACT: LGR5 was significantly over expressed in colorectal cancer compared to normal tissues. LGR5 was noted to be an independent prognostic variable for an improved survival outcome in colorectal cancer patients with peritoneal metastasis, making LGR5 a potential therapeutic target in colorectal cancer patients with peritoneal metastasis.

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Associations between physical activity, sedentary behavior, and urinary oxidized guanine in colorectal cancer patients: results from the ColoCare Study

Applied Physiology Nutrition and Metabolism ◽

10.1139/apnm-2019-0836 ◽

2020 ◽

Vol 45 (11) ◽

pp. 1306-1309

Author(s):

Richard Viskochil ◽

Biljana Gigic ◽

Tengda Lin ◽

Stephanie Skender ◽

Jürgen Böhm ◽

...

Keyword(s):

Oxidative Stress ◽

Physical Activity ◽

Colorectal Cancer ◽

Sedentary Behavior ◽

Cancer Patients ◽

Partial Correlations ◽

Oxidized Guanine ◽

Colorectal Cancer Patients ◽

Objectively Measured ◽

Study Participants

To determine associations between physical activity (PA), sedentary behavior (SB), and oxidative stress in colorectal cancer patients, ColoCare Study participants in Germany wore an accelerometer 6 and/or 12 months after surgery. Spearman partial correlations were used to assess associations between PA and urinary concentrations of oxidized guanine, a validated marker of oxidative stress. There were no significant associations between PA or SB and oxidized guanine in n = 76 measurements (ng/mg creatinine; r = 0.03, p = 0.76 for PA, r = –0.05, p = 0.69 for SB). Novelty Objectively measured PA was not associated with a marker of oxidative stress in colorectal cancer patients.

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