scholarly journals Liver Damage After Radiofrequency Ablation Combined with Transcatheter Therapy in Treating Rabbit VX2 Liver Tumors

2020 ◽  
Author(s):  
Xu-Hua Duan ◽  
Wen-Hui Wang ◽  
Xinwei Han ◽  
Jian-Zhuang Ren ◽  
Hao Li ◽  
...  
Keyword(s):  
Radiofrequency Ablation ◽  
Liver Injury ◽  
Liver Tumors ◽  
Tumor Model ◽  
Arterial Embolization ◽  
Hepatocyte Proliferation ◽  
Control Group ◽  
Tumor Control ◽  
Hepatocellular Necrosis ◽  
Vx2 Tumor

Abstract Background: To evaluate the effect of transcatheter therapies combining with radiofrequency ablation (RFA) treatment on hepatocellular necrosis, apoptosis and proliferation by using the rabbit VX2 tumor model. Methods: Ninety six models were randomly divided into 4 groups: transcatheter arterial chemoembolization group (TACE), radiofrequency ablation group (RFA), TACE+RFA group and transcatheter arterial embolization (TAE) + RFA group. The above groups were further divided into two subgroups, A (15 rabbits) and B (9 rabbits). The subgroup B (control group) was followed up until animal death. Results: The high expression of heat shock protein 70 (HSP70) was observed in the adjacent liver tissue in TACE and TACE+RFA. The highest increase of transaminase levels and serum HSP70 were detected in TACE+RFA group. TAE+RFA group had a low apoptotic rate and more hepatocyte proliferation as compared to TACE and TACE+RFA groups; and it had the longest end-point survival among these groups. Conclusions: The TAE+RFA treatment had a better outcome than RFA, including a better liver tumor control, a less liver injury, and a longer survival than TACE+RFA. Compared to TACE and TACE+RFA procedures, TAE+RFA significantly decreased the liver injury, hepatocellular necrosis, apoptosis and systemic proinflammatory cytokine release caused by anticancer drugs application.

scholarly journals PL-019 Effect of early exercise on autophagy of liver tumor in mice

2018 ◽  
Vol 1 (1) ◽  
Author(s):  
Ning Jiang ◽  
Zhe Wang ◽  
Jing Li ◽  
Xinghao Wang
Keyword(s):  
Exercise Training ◽  
Liver Tumor ◽  
Liver Tumors ◽  
Hepatoma Cell ◽  
Tumor Model ◽  
Inflammatory Cells ◽  
Exercise Group ◽  
Control Group ◽  
Early Exercise ◽  
Tumor Group

Objective To investigate whether the liver autophagy level can be altered by pre exercise training in mice liver tumors. Methods 40 Male C57BL/6J mice aged 7 months were randomly divided into 2 groups: control group (YC) and exercise group (YE). YE were exercised on a treadmill for 12 weeks (12m/min). After12 weeks each group was randomly divided into two groups. The tumor model was constructed by injection of HEPA1- 6 mouse hepatoma cell into liver tissue.Then the groups were control group (YC), exercise group (YE), tumor group (YCT), exercise tumor group (YET).The experimental samples were prepared on the 13 day after the tumor model was constructed. the hematoxylin and eosin stain of the liver was observed.The expression of autophagy related protein BECLIN1, LC3-II and ATG5 in liver tissues of mice was detected by Western blot. Results Compared with YCT group,the boundary of inflammatory cells and tumor cells in YET group was clear with normal cells.Compared with YCT group, the expression levels of BECLIN1, LC3-II and ATG5 in liver tissue of YET group were significantly higher (p < 0.01, P < 0.01, P < 0.05). Conclusions Early exercise can help the 7 month old mice to resist the occurrence and development of the liver tumor. It's probably associated with increased level of autophagy in the liver by early exercise training.

scholarly journals Antitumor Effects of Intra-Arterial Delivery of Albumin-Doxorubicin Nanoparticle Conjugated Microbubbles Combined with Ultrasound-Targeted Microbubble Activation on VX2 Rabbit Liver Tumors

Cancers ◽  
2019 ◽  
Vol 11 (4) ◽  
pp. 581 ◽  
Author(s):  
Lee ◽  
Moon ◽  
Han ◽  
Lee ◽  
Kim ◽  
...  
Keyword(s):  
Liver Toxicity ◽  
Liver Tumors ◽  
Tumor Model ◽  
Control Group ◽  
Antitumor Effects ◽  
Ultrasound Exposure ◽  
Therapeutic Efficiency ◽  
Free Doxorubicin ◽  
Adc Values ◽  
Hepatic Malignancies

Image-guided intra-arterial therapies play a key role in the management of hepatic malignancies. However, limited clinical outcomes suggest the need for new multifunctional drug delivery systems to enhance local drug concentration while reducing systemic adverse reactions. Therefore, we developed the albumin-doxorubicin nanoparticle conjugated microbubble (ADMB) to enhance therapeutic efficiency by sonoporation under exposure to ultrasound. ADMB demonstrated a size distribution of 2.33 ± 1.34 µm and a doxorubicin loading efficiency of 82.7%. The echogenicity of ADMBs was sufficiently generated in the 2–9 MHz frequency range and cavitation depended on the strength of the irradiating ultrasound. In the VX2 rabbit tumor model, ADMB enhanced the therapeutic efficiency under ultrasound exposure, compared to free doxorubicin. The intra-arterial administration of ADMBs sufficiently reduced tumor growth by five times, compared to the control group. Changes in the ADC values and viable tumor fraction supported the fact that the antitumor effect of ADMBs were enhanced by evidence of necrosis ratio (over 70%) and survival tumor cell fraction (20%). Liver toxicity was comparable to that of conventional therapies. In conclusion, this study shows that tumor suppression can be sufficiently maximized by combining ultrasound exposure with intra-arterial ADMB administration.

scholarly journals Early Assessment of Response to Radiofrequency Ablation With CT Perfusion Imaging in Rabbit VX2 Liver Tumor Model

2021 ◽  
Vol 11 ◽  
Author(s):  
Xiaofei Yue ◽  
Xiangjun Dong ◽  
Mengting Huang ◽  
Hongli Yang ◽  
Kun Qian ◽  
...  
Keyword(s):  
Radiofrequency Ablation ◽  
High Efficiency ◽  
Liver Tumors ◽  
Ct Perfusion ◽  
Early Stage ◽  
Liver Perfusion ◽  
Tumor Model ◽  
Diagnostic Value ◽  
Early Assessment ◽  
Speed Up

ObjectivesTo discriminate viable tumors from benign periablational enhancement (BPE) in early stage after radiofrequency ablation (RFA) is a major confounding problem. The goal of this study is to evaluate quantitative assessment and diagnostic value of CT perfusion between viable tumors and BPE after RFA in the rabbit liver VX2 tumor model, with pathological results as the standard.MethodsTwenty-eight VX2 liver tumors were treated with RFA, on days 1, 3, 7, and 14, seven rabbits were randomly chosen for CT perfusion and performed pathology examinations immediately. The perfusion parameters along with the profile of time-density curves (TDCs) and pseudo-color images of the parameters were observed in both BPE and viable tumors, then compared with the pathology results. The perfusion parameters included blood flow (BF), blood volume (BV), time to peak (TTP), permeability (P), arterial liver perfusion (ALP), portal venous perfusion (PVP) and hepatic perfusion index (HPI).ResultsA total of 26/28 rabbits successfully underwent CT perfusion, while 6/26 lesions were confirmed to be viable tumors. The TDCs of BPE were mainly speed-up platform curves (15/26), while the viable tumors showed mainly speed-up speed-down (3/6) and speed-up platform (2/6) curves. The PVP values were significantly higher, and the HPI values were significantly lower for BPE at all time points than viable tumors (P &lt; 0.05). Both of PVP value and HPI value have high efficiency for the differential diagnosis of the viable tumors and BPE at each time point. These characteristics of CT perfusion parameters were consistent with pathological changes.ConclusionsThe TDCs, PVP and HPI have the potential to indicate BPE and viable tumors effectively early after RFA treatment, the results were highly consistent with pathology. CT perfusion has advantages with great efficacy in monitoring the therapeutic effect early after RFA treatment.

Percutaneous US-guided Radiofrequency Ablation with Perfused Needle Applicators: Improved Survival with the VX2 Tumor Model in Rabbits

Radiology ◽  
2004 ◽  
Vol 230 (1) ◽  
pp. 169-174 ◽  
Author(s):  
Johannes Hänsler ◽  
Daniel Neureiter ◽  
Milan Wasserburger ◽  
Rolf Janka ◽  
Thomas Bernatik ◽  
...  
Keyword(s):  
Radiofrequency Ablation ◽  
Tumor Model ◽  
Vx2 Tumor

Radiofrequency Ablation: Effect of Pharmacologic Modulation of Hepatic and Renal Blood Flow on Coagulation Diameter in a VX2 Tumor Model

2004 ◽  
Vol 15 (3) ◽  
pp. 269-274 ◽  
Author(s):  
Clare Horkan ◽  
Muneeb Ahmed ◽  
Zhengjun Liu ◽  
G. Scott Gazelle ◽  
Stephanie A. Solazzo ◽  
...  
Keyword(s):  
Blood Flow ◽  
Radiofrequency Ablation ◽  
Renal Blood Flow ◽  
Tumor Model ◽  
Vx2 Tumor ◽  
Pharmacologic Modulation

scholarly journals Laser-Induced Thermotherapy Combined With Hepatic Arterial Embolization in the Treatment of Liver Tumors in a Rat Tumor Model

1999 ◽  
Vol 230 (1) ◽  
pp. 55 ◽  
Author(s):  
Christoph- T. Germer ◽  
Christoph Isbert ◽  
Dirk Albrecht ◽  
André Roggan ◽  
Jörg Pelz ◽  
...  
Keyword(s):  
Liver Tumors ◽  
Tumor Model ◽  
Arterial Embolization ◽  
Rat Tumor Model ◽  
Hepatic Arterial Embolization ◽  
Rat Tumor

scholarly journals Effect of vascular occlusion on radiofrequency ablation of the liver in a rabbit VX2 tumor model

2009 ◽  
Vol 17 (4) ◽  
pp. 352
Author(s):  
Jian-Bo Han ◽  
Yu-Dong Qiu ◽  
Wei-Wei Zhang ◽  
Wen-Tao Kong ◽  
Jun-Lan Qiu ◽  
...  
Keyword(s):  
Radiofrequency Ablation ◽  
Tumor Model ◽  
Vascular Occlusion ◽  
Vx2 Tumor

Phase I dose escalation and PK study of thermally sensitive liposomes containing doxorubicin given during radiofrequency ablation (RFA) in patients with non-resectable primary and metastatic liver cancer

2007 ◽  
Vol 25 (18_suppl) ◽  
pp. 15010-15010 ◽  
Author(s):  
B. J. Wood ◽  
R. T. Poon ◽  
Z. Neeman ◽  
M. Eugeni ◽  
J. Locklin ◽  
...  
Keyword(s):  
Radiofrequency Ablation ◽  
Liver Cancer ◽  
Phase I ◽  
Dose Escalation ◽  
Liver Tumors ◽  
Ct Scans ◽  
Tumor Control ◽  
Metastatic Liver Cancer ◽  
Contrast Enhanced Ct ◽  
Metastatic Liver

15010 Purpose: This phase I dose escalation and pharmacokinetic (PK) study in patients with non-resectable primary or metastatic hepatic tumors undergoing radiofrequency ablation (RFA) uses a 30-minute IV infusion of ThermoDox (TDox) starting 15 minutes prior to RFA treatment. TDox liposomes are engineered to release doxorubicin (Dox) locally at temperatures greater than 39.5 °C. High local concentrations of Dox could allow for increased drug concentration targeted at the tumor margins in an effort to achieve improved local recurrence and tumor control near these RFA-induced thermal lesions. The phase I study goals are to determine the maximum tolerated dose and dose-limiting toxicity of TDox. Patients and Methods: Patients (pts) must be eligible for RFA for primary (HCC) or metastatic liver cancer (MLC). Main inclusion criteria are = 4 lesions and = 7 cm in greatest diameter. Dose escalation is: cohorts of 3–6 pts treated with a single dose of 20, 30, 40, 50, 60 or 70 mg/m2. RFA is administered via percutaneous or surgical approach. RFA treatment without TDox can be repeated for recurrent hepatic (distant or local) tumors. Patients requiring systemic chemotherapy following RFA are removed from the study. MRI, PET and contrast enhanced CT (CE-CT) scans are done pre-, one and three months post-treatment (q3 months thereafter for patients on trial). CE-CT scans are also performed immediately following RFAs. Patients are assessed for safety, PK, and lesion diameters on CT. RFA+TDox lesion diameters will be compared to patients treated by RFA alone (control) at the same institution. Results: A total of 22 pts have been treated as of January 2007 submission date (3, 6, 6, 6, 1 patients at 20, 30, 40, 50, and 60 mg/m2, respectively). This population includes 8 pts with HCC and 14 pts with MLC. Grade 3/4 toxicity (reversible neutropenia) has been observed to be dose dependent. 1 patient at 50 mg/m2 has met DLT criteria. Conclusions: TDox has been safely administered in combination with percutaneous or surgical RFA procedures in 22 patients with liver tumors. There has been limited, manageable toxicity thus far. Enrollment continues as the MTD and DLT have yet to be defined. [Table: see text]

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