Using A Combination Of Chitosan, Methyl Jasmonate, and Cyclodextrin as an Effective Elicitation Strategy for Prenylated Stilbene Compound Production In Arachis Hypogaea L. Hairy Root Culture and Their Impact on Genomic DNA

Abstract Peanut (Arachis hypogaea L.) hairy roots are a potential tool for stilbene compound production. This study focuses on the most efficient elicitation strategy for inducing stilbene compound production, especially for the prenylated forms of trans-arachidin-1 and trans-arachidin-3. To the best of our knowledge, this is the first report of simultaneous treatment involving chitosan (CHT), methyl jasmonate (MeJA), and cyclodextrin (CD); CHT+MeJA+CD in peanut hairy roots to induce substantial amounts of trans-arachidin-1 and trans-arachidin-3 at 72 h of the elicitation period. The results demonstrate the highest amounts of trans-arachidin-1 and trans-arachidin-3, with 684.30 ± 183.85 and 543.94 ± 171.17 mg/g dry weight of hairy roots, respectively. The highest antioxidant capacity determined by ABTS and FRAP assays was 188.95 ± 0.43 µmol Trolox/g dry weight of hairy roots and 24.61 ± 0.50 mg ascorbic acid/g dry weight of hairy roots, respectively, while the highest phenolic content was also detected in this crude extract, with 8.67 ± 0.11 mg gallic acid/g dry weight of hairy roots. The antioxidant and pro-oxidant activity of the CHT+MeJA+CD crude extract in the protection and damage of DNA is of great interest and can have major positive impacts on health promotion and disease prevention.

Download Full-text

Using a combination of chitosan, methyl jasmonate, and cyclodextrin as an effective elicitation strategy for prenylated stilbene compound production in Arachis hypogaea L. hairy root culture and their impact on genomic DNA

Plant Cell Tissue and Organ Culture (PCTOC) ◽

10.1007/s11240-021-02112-4 ◽

2021 ◽

Author(s):

Phadtraphorn Chayjarung ◽

Wannakan Poonsap ◽

Chanyanut Pankaew ◽

Onrut Inmano ◽

Anupan Kongbangkerd ◽

...

Keyword(s):

Methyl Jasmonate ◽

Arachis Hypogaea ◽

Hairy Root ◽

Genomic Dna ◽

Root Culture ◽

Hairy Root Culture ◽

Arachis Hypogaea L

Download Full-text

Effects of methyl jasmonate on the growth and triterpenoid production of diploid and tetraploid Centella asiatica (L.) Urb. hairy root cultures

Scientific Reports ◽

10.1038/s41598-019-54460-z ◽

2019 ◽

Vol 9 (1) ◽

Author(s):

Khoa Van Nguyen ◽

Benyakan Pongkitwitoon ◽

Thanika Pathomwichaiwat ◽

Unchera Viboonjun ◽

Sompop Prathanturarug

Keyword(s):

Methyl Jasmonate ◽

Hairy Root ◽

Hairy Roots ◽

Centella Asiatica ◽

Root Culture ◽

Hairy Root Culture ◽

Asiatic Acid ◽

Hairy Root Cultures ◽

Root Cultures ◽

Meja Treatment

AbstractIn this study, the effects of methyl jasmonate (MeJA) on the phytomass and triterpenoid production of diploid and tetraploid Centella asiatica hairy roots were investigated. Hairy root cultures were obtained from diploid and induced tetraploid plants of C. asiatica infected by Agrobacterium rhizogenes strain ATCC 43057. MeJA triggered triterpenoid production in both ploidy hairy roots, whereas triterpenoids were not produced in the untreated hairy roots. Among the treatments, the 50 µM MeJA treatment yielded the maximum triterpenoid production in diploid hairy roots of 27.25 ± 0.27 µg/mg Dry weight (DW) total triterpenoid at day 21. For the tetraploid hairy root cultures, the 28th-day hairy root culture produced a maximum amount of triterpenoids of 16.29 ± 6.32 µg/mg DW in response to the 50 µM MeJA treatment, whereas the 100 µM MeJA treatment produced a similar triterpenoid amount (16.31 ± 9.24 µg/mg DW) at day 14. Moreover, in response to 50 µM MeJA, we obtained different ratios of aglycone to glycoside, i.e., 1:7 and 1:2, between the diploid and tetraploid hairy root cultures. Asiaticoside was the dominant phytochemical, followed by asiatic acid and madecassic acid. This study provides valuable information for producing triterpenoids for C. asiatica commercial products and preparations by using hairy root cultures.

Download Full-text

Effect of methyl jasmonate on the production of sesquiterpene lactones in the hairy root culture of Lactuca virosa L.

Acta Societatis Botanicorum Poloniae ◽

10.5586/asbp.1999.017 ◽

2014 ◽

Vol 68 (2) ◽

pp. 119-121 ◽

Cited By ~ 4

Author(s):

Janusz Malarz ◽

Wanda Kisiel

Keyword(s):

Methyl Jasmonate ◽

Agrobacterium Rhizogenes ◽

Hairy Root ◽

Hairy Roots ◽

Hplc Analysis ◽

Sesquiterpene Lactones ◽

Root Culture ◽

Hairy Root Culture ◽

Lactuca Virosa ◽

Methyl Jasmonate Treatment

Hairy roots of <em>Lactuca virosa</em> L. transformed with <em>Agrobacterium rhizogenes</em> and treated with methyl jasmonate, are capable of producing sesquiterpene lactones - crepidiaside B and lactuside A in amounts higher than in untreated roots. An HPLC analysis showed the highest content of crepidiaside B after 24 hours of methyl jasmonate treatment (60% above the control). The increase in the content of lactuside A was relatively small and reached 27% after 48 hours of the treatment, in comparison with the control.

Download Full-text

A reliable and efficient protocol for induction of hairy roots in Agastache foeniculum

Biologia ◽

10.2478/s11756-014-0382-8 ◽

2014 ◽

Vol 69 (7) ◽

Cited By ~ 16

Author(s):

Elnaz Nourozi ◽

Bahman Hosseini ◽

Abbas Hassani

Keyword(s):

Salicylic Acid ◽

Agrobacterium Rhizogenes ◽

Hairy Root ◽

Hairy Roots ◽

High Performance ◽

Root Culture ◽

Hairy Root Culture ◽

Biochemical Properties ◽

Separate Experiment ◽

Transformed Roots

AbstractHairy root culture system is a valuable tool to study the characteristics of gene expression, gene function, root biology, biochemical properties and biosynthesis pathways of secondary metabolites. In the present study, hairy roots were established in Anise hyssop (Agastache foeniculum) via Agrobacterium rhizogenes. Three strains of Agrobacterium rhizogenes (A4, A7 and 9435), were used for induction of hairy roots in four various explants (hypocotyl, cotyledon, one-month-old leaf and five-month-old leaf) of Anise hyssop. The highest frequency of transformation was achieved using A4 strain in one-month-old leaves (51.1%). The transgenic states of hairy root lines were confirmed by PCR (Polymerase chain reaction) method. High performance liquid chromatography analysis revealed that the production of rosmarinic acid (RA) in transformed roots of A. foeniculum was almost 4-fold higher than that of the non-transformed roots. In a separate experiment, hairy roots obtained from one-month-old leaves inoculated with A4 strain, were grown in liquid medium and the effects of different concentrations of salicylic acid (0.0, 0.01, 0.1 and 1 mM) and chitosan (0, 50, 100 and 150 mg L−1) (as elicitor) and sucrose (20, 30, 40 and 50 g L−1) on the growth of hairy roots were evaluated. The results showed that, 30 g L−1 sucrose and 100 mg L−1 chitosan increased the biomass of hairy root cultures and application of salicylic acid reduced the growth of hairy roots compared with control roots.

Download Full-text

Technology and Application of Hairy Root Culture in Monocotyledons

Current Biochemical Engineering ◽

10.2174/2212711907666210525115018 ◽

2021 ◽

Vol 07 ◽

Author(s):

Chang-Qi Hao ◽

Shuai-Run Wang ◽

Yi Wang ◽

Xin-Yi Hou ◽

Ya-Xuan Jiang ◽

...

Keyword(s):

Medicinal Plants ◽

Agrobacterium Rhizogenes ◽

Hairy Root ◽

Hairy Roots ◽

Root Culture ◽

Hairy Root Culture ◽

Food Crops ◽

Root Induction ◽

Hairy Root Induction ◽

Selection Of

Background: Hairy root culture has been widely used in the production of metabolites in dicotyledons, and a large number of food crops and medicinal plants in monocotyledons need to be developed, but there are many difficulties in the induction of hairy roots in monocotyledons. The purpose of this paper is to introduce the inducing methods, influencing factors and application of hairy roots in monocotyledons, and to promote the development of hairy root system in monocotyledons. Methods: The mechanism of action of Agrobacterium rhizogenes and the current situation of hairy root induction, induction methods and influencing factors of monocotyledons were summarized so as to provide convenience for efficient acquisition of hairy root of monocotyledons. Results: Monocotyledons are not easy to produce phenols, cells are prone to lignification, adverse differentiation and selective response to Agrobacterium rhizogenes strains. It is proposed that before induction, plant varieties and explants should be selected, and different infection strains should be screened. In the process of hairy root induction, exogenous inducers such as acetosyringone can be added. Although these factors can provide some help for the induction of hairy roots in monocotyledons, we still need to pay attention to the disadvantages of monocotyledons from dicotyledons at the cellular level. Conclusion: A large number of food crops and medicinal plants are monocotyledons. Hairy root culture can be used to help the breeding and production of medicinal substances. Therefore, it is necessary to pay attention to the selection of varieties and explants, the selection of Agrobacterium rhizogenes and the addition of acetosyringone in the process of hairy root induction so as to improve the production efficiency and facilitate the development and utilization of monocotyledons.

Download Full-text

Hairy Roots

Advances in Environmental Engineering and Green Technologies - Molecular Plant Breeding and Genome Editing Tools for Crop Improvement ◽

10.4018/978-1-7998-4312-2.ch008 ◽

2021 ◽

pp. 219-252

Keyword(s):

Secondary Metabolites ◽

Hairy Root ◽

Hairy Roots ◽

High Growth ◽

Root Culture ◽

Hairy Root Culture ◽

High Growth Rate ◽

Hairy Root Cultures ◽

Root Cultures ◽

Transformed Root Cultures

Agrobacterium rhizogenes induces hairy root disease in plants. The neoplastic (cancerous) roots produced by A. rhizogenes infection, when cultured in hormone free medium, show high growth rate and genetic stability. These genetically transformed root cultures can produce levels of secondary metabolites comparable to that of intact plants. Several elicitation methods can be used to further enhance the production and accumulation of secondary metabolites. Thus, hairy root culture offer promise for high production and productivity of valuable secondary metabolites in many plants. Hairy roots can also produce recombinant proteins from transgenic roots, and thereby hold immense potential for pharmaceutical industry. Hairy root cultures can be used to elucidate the intermediates and key enzymes involved in the biosynthesis of secondary metabolites, and for phytoremediation due to their abundant neoplastic root proliferation property. Various applications of hairy root cultures and potential problems associated with them are discussed in this chapter.

Download Full-text

Effects of Bradyrhizobium strain and host genotype, nodule dry weight and leaf area on groundnut (Arachis hypogaea L. ssp. fastigiata) yield

Plant and Soil ◽

10.1007/bf00012533 ◽

1993 ◽

Vol 154 (2) ◽

pp. 279-288 ◽

Cited By ~ 10

Author(s):

Diman Van Rossum ◽

Arthur Muyotcha ◽

Henk W. Van Verseveld ◽

Adriaan H. Stouthamer ◽

Fred C. Boogerd

Keyword(s):

Leaf Area ◽

Arachis Hypogaea ◽

Dry Weight ◽

Host Genotype ◽

Bradyrhizobium Strain ◽

Arachis Hypogaea L

Download Full-text

Hairy Root Cultures of Eurycoma longifolia and Production of Anti-inflammatory 9-Methoxycanthin-6-one

Natural Product Communications ◽

10.1177/1934578x1801300507 ◽

2018 ◽

Vol 13 (5) ◽

pp. 1934578X1801300

Author(s):

Trang Thu Tran ◽

Nam Trung Nguyen ◽

Ngoc Bich Pham ◽

Huy Nhat Chu ◽

Trong Dinh Nguyen ◽

...

Keyword(s):

Hairy Root ◽

Root Culture ◽

Dry Weight ◽

Hairy Root Culture ◽

Peritoneal Macrophages ◽

High Yield ◽

Hairy Root Cultures ◽

Root Cultures ◽

Eurycoma Longifolia ◽

Anti Inflammatory

The content of bioactive alkaloid 9-methoxycanthin-6-one in the roots of Eurycoma longifolia is rather small. In the current study, a high yield of 9-methoxycanthin-6-one was obtained from transgenic hairy roots (1.139 ± 0.20% dry weight, DW) compared to wild roots (0.164 ± 0.25% DW) of E. longifolia. Three liquid basal media supplemented with 3% sucrose were tested for the growth and accumulation of 9-methoxycanthin-6-one. The fastest growth was obtained in the Shenck and Hildebrandt (SH) medium while the highest 9-methoxycanthin-6-one content was observed in McCown's woody plant (WP) medium. In this study, compared to controls, the production of 9-methoxycanthin-6-one was increased by the maximum of 2.6 and 4.0 times after the addition of jasmonic acid and yeast extract, respectively, into hairy root cultures. In addition, pro-inflammatory cytokines IL-6 and TNF-α were significantly inhibited by 9-methoxycanthin-6-one in lipopolysaccharide-stimulated murine RAW264.7 cells, peritoneal macrophages and human THP-1 macrophages These results suggest that the elicited hairy root culture of E. longifolia is an alternative system for the production of an abundant source of anti-inflammatory 9-methoxycanthin-6-one.

Download Full-text

Polyprenols in hairy roots of Coluria geoides

Biochemical Society Transactions ◽

10.1042/bst0280790 ◽

2000 ◽

Vol 28 (6) ◽

pp. 790-791 ◽

Cited By ~ 5

Author(s):

K. Skorupińska-Tudek ◽

V. S. Hung ◽

O. Olszowska ◽

M. Furmanowa ◽

T. Chojnacki ◽

...

Keyword(s):

Agrobacterium Rhizogenes ◽

Hairy Root ◽

Lipid Content ◽

Mammalian Cells ◽

Root Culture ◽

Dry Weight ◽

Hairy Root Culture ◽

Living Organisms ◽

Long Chain

Long-chain polyisoprenoid alcohols built from several up to more than 100 isoprenoid units are common constituents of all living organisms. They were found mostly in plants, bacteria, yeasts and mammalian cells. In vitro hairy root culture of Coluria geoides was obtained from plants transformed with Agrobacterium rhizogenes. Growth was optimal at 0.75% (w/v) glucose and at 22 °C. Dry samples of roots were extracted and lipid content was analysed by HPLC. According to our estimation, polyprenols are accumulated in roots of C. geoides cultivated in vitro as a mixture of several prenologues with the dominating prenol composed of 16 isoprenoid units. The content of polyprenols in tissue was approx. 300 μg/g of dry weight.

Download Full-text