scholarly journals A Novel Quantification Platform for Point-of-care testing of Circulating MicroRNAs Based on Allosteric Spherical Nanoprobe

2020 ◽  
Author(s):  
Huiyan Tian ◽  
Changjing Yuan ◽  
Yu Liu ◽  
Zhi Li ◽  
Ke Xia ◽  
...  
Keyword(s):  
Self Assembly ◽  
Point Of Care ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Reaction Cross ◽  
Detection Sensitivity ◽  
Point Of Care Testing ◽  
Bioanalytical Application ◽  
Target Probe ◽  
Nsclc Patients

Abstract MiRNA-150, a gene regulator that has been revealed to be abnormal expression in non-small cell lung cancer ( NSCLC ), can be regarded as a serum indicator for diagnosis and monitoring of NSCLC . Herein, a new sort of nanoprobe, termed allosteric spherical nanoprobe, was first developed to sense miRNA-150. Compared with conventional hairpin, this new nanoprobe possesses more enrichment capacity and reaction cross section. Structurally, it consists of magnetic nanoparticles and dual-hairpin. In the absence of miRNA-150, the spherical nanoprobes form hairpin structure through DNA self-assembly, which could promote the Förster resonance energy transfer (FRET) of fluorophore (FAM) and quencher (BHQ1) nearby. However, in the presence of target, the target-probe hybridization can open the hairpin and form the active “Y” structure which separated fluorophore and quencher to yield a “signal on” fluorescence. In the manner of multipoint fluorescence detection , the target-bound allosteric spherical nanoprobe could provide a high detection sensitivity with a linear range of 100 fM to 10 nM and a detection limit of 38 fM. More importantly, the proposed method could distinguish the expression of serum miRNA-150 among NSCLC patients and healthy people. Finally, we hoped that the potential bioanalytical application of this nanoprobe strategy will pave the way for point-of-care testing (POCT).

scholarly journals A novel quantification platform for point-of-care testing of circulating MicroRNAs based on allosteric spherical nanoprobe

2020 ◽  
Vol 18 (1) ◽  
Author(s):  
Huiyan Tian ◽  
Changjing Yuan ◽  
Yu Liu ◽  
Zhi Li ◽  
Ke Xia ◽  
...  
Keyword(s):  
Self Assembly ◽  
Point Of Care ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Reaction Cross ◽  
Detection Sensitivity ◽  
Point Of Care Testing ◽  
Bioanalytical Application ◽  
Target Probe ◽  
Nsclc Patients

Abstract MiRNA-150, a gene regulator that has been revealed to be abnormal expression in non-small cell lung cancer (NSCLC), can be regarded as a serum indicator for diagnosis and monitoring of NSCLC. Herein, a new sort of nanoprobe, termed allosteric spherical nanoprobe, was first developed to sense miRNA-150. Compared with conventional hairpin, this new nanoprobe possesses more enrichment capacity and reaction cross section. Structurally, it consists of magnetic nanoparticles and dual-hairpin. In the absence of miRNA-150, the spherical nanoprobes form hairpin structure through DNA self-assembly, which could promote the Förster resonance energy transfer (FRET) of fluorophore (FAM) and quencher (BHQ1) nearby. However, in the presence of target, the target-probe hybridization can open the hairpin and form the active “Y” structure which separated fluorophore and quencher to yield “signal on” fluorescence. In the manner of multipoint fluorescence detection, the target-bound allosteric spherical nanoprobe could provide high detection sensitivity with a linear range of 100 fM to 10 nM and a detection limit of 38 fM. More importantly, the proposed method can distinguish the expression of serum miRNA-150 among NSCLC patients and healthy people. Finally, we hoped that the potential bioanalytical application of this nanoprobe strategy will pave the way for point-of-care testing (POCT).

scholarly journals A Sample-to-Answer Quantification Platform for Point-of-Care Testing of Circulating MicroRNAs Based on Allosteric Spherical Nanoprobe

2020 ◽  
Author(s):  
Huiyan Tian ◽  
Changjing Yuan ◽  
Yu Liu ◽  
Ke Xia ◽  
Mengya Li ◽  
...  
Keyword(s):  
Self Assembly ◽  
Point Of Care ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Reaction Cross ◽  
Detection Sensitivity ◽  
Point Of Care Testing ◽  
Bioanalytical Application ◽  
Target Probe ◽  
Nsclc Patients

Abstract MiRNA-150, a gene regulator that has been revealed to be abnormal expression in non-small cell lung cancer (NSCLC), can be regarded as a serum indicator for diagnosis and monitoring of NSCLC. Herein, a new sort of nanoprobe, termed allosteric spherical nanoprobe, was first developed to sense miRNA-150. Compared with conventional hairpin, this new nanoprobe possesses more enrichment capacity and reaction cross section. Structurally, it consists of magnetic nanoparticles and dual-hairpin. In the absence of miRNA-150, the spherical nanoprobes form hairpin structure through DNA self-assembly, which could promote the Förster resonance energy transfer (FRET) of fluorophore (FAM) and quencher (BHQ1) nearby. However, in the presence of target, the target-probe hybridization can open the hairpin and form the active “Y” structure which separated fluorophore and quencher to yield a “signal on” fluorescence. In the manner of multipoint fluorescence detection, the target-bound allosteric spherical nanoprobe could provide a high detection sensitivity with a linear range of 100 fM to 10 nM and a detection limit of 38 fM. More importantly, the proposed method can distinguish the expression of serum miRNA-150 among NSCLC patients and healthy people. Finally, we hoped that the potential bioanalytical application of this nanoprobe strategy will pave the way for point-of-care testing (POCT).

Programmable in vitro Co-Encapsidation of Guest Proteins Inside Protein Nanocages

2018 ◽  
Author(s):  
Noor H. Dashti ◽  
Rufika S. Abidin ◽  
Frank Sainsbury
Keyword(s):  
Self Assembly ◽  
Mammalian Cells ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Super Resolution ◽  
Cell Engineering ◽  
Particle Analysis ◽  
Protein Cages

Bioinspired self-sorting and self-assembling systems using engineered versions of natural protein cages have been developed for biocatalysis and therapeutic delivery. The packaging and intracellular delivery of guest proteins is of particular interest for both <i>in vitro</i> and <i>in vivo</i> cell engineering. However, there is a lack of platforms in bionanotechnology that combine programmable guest protein encapsidation with efficient intracellular uptake. We report a minimal peptide anchor for <i>in vivo</i> self-sorting of cargo-linked capsomeres of the Murine polyomavirus (MPyV) major coat protein that enables controlled encapsidation of guest proteins by <i>in vitro</i> self-assembly. Using Förster resonance energy transfer (FRET) we demonstrate the flexibility in this system to support co-encapsidation of multiple proteins. Complementing these ensemble measurements with single particle analysis by super-resolution microscopy shows that the stochastic nature of co-encapsidation is an overriding principle. This has implications for the design and deployment of both native and engineered self-sorting encapsulation systems and for the assembly of infectious virions. Taking advantage of the encoded affinity for sialic acids ubiquitously displayed on the surface of mammalian cells, we demonstrate the ability of self-assembled MPyV virus-like particles to mediate efficient delivery of guest proteins to the cytosol of primary human cells. This platform for programmable co-encapsidation and efficient cytosolic delivery of complementary biomolecules therefore has enormous potential in cell engineering.

scholarly journals Theoretical Modeling of Viscosity Monitoring with Vibrating Resonance Energy Transfer for Point-of-Care and Environmental Monitoring Applications

Micromachines ◽  
2018 ◽  
Vol 10 (1) ◽  
pp. 3 ◽  
Author(s):  
Gorkem Memisoglu ◽  
Burhan Gulbahar ◽  
Joseba Zubia ◽  
Joel Villatoro
Keyword(s):  
Energy Transfer ◽  
Environmental Monitoring ◽  
System Design ◽  
Point Of Care ◽  
Resonance Energy Transfer ◽  
Microfluidic Channel ◽  
Resonance Energy ◽  
Polluted Water ◽  
Hardware Complexity ◽  
Monitoring Applications

Förster resonance energy transfer (FRET) between two molecules in nanoscale distances is utilized in significant number of applications including biological and chemical applications, monitoring cellular activities, sensors, wireless communications and recently in nanoscale microfluidic radar design denoted by the vibrating FRET (VFRET) exploiting hybrid resonating graphene membrane and FRET design. In this article, a low hardware complexity and novel microfluidic viscosity monitoring system architecture is presented by exploiting VFRET in a novel microfluidic system design. The donor molecules in a microfluidic channel are acoustically vibrated resulting in VFRET in the case of nearby acceptor molecules detected with their periodic optical emission signals. VFRET does not require complicated hardware by directly utilizing molecular interactions detected with the conventional photodetectors. The proposed viscosity measurement system design is theoretically modeled and numerically simulated while the experimental challenges are discussed. It promises point-of-care and environmental monitoring applications including viscosity characterization of blood or polluted water.

scholarly journals Recent Developments of Electrochemical and Optical Biosensors for Antibody Detection

2019 ◽  
Vol 21 (1) ◽  
pp. 134 ◽  
Author(s):  
Wei Xu ◽  
Daniel Wang ◽  
Derek Li ◽  
Chung Chiun Liu
Keyword(s):  
Point Of Care ◽  
Low Cost ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Disease Diagnosis ◽  
Antibody Detection ◽  
Recent Developments ◽  
Care Systems ◽  
Point Of Care Systems ◽  
Detection Of Biomarkers

Detection of biomarkers has raised much interest recently due to the need for disease diagnosis and personalized medicine in future point-of-care systems. Among various biomarkers, antibodies are an important type of detection target due to their potential for indicating disease progression stage and the efficiency of therapeutic antibody drug treatment. In this review, electrochemical and optical detection of antibodies are discussed. Specifically, creating a non-label and reagent-free sensing platform and construction of an anti-fouling electrochemical surface for electrochemical detection are suggested. For optical transduction, a rapid and programmable platform for antibody detection using a DNA-based beacon is suggested as well as the use of bioluminescence resonance energy transfer (BRET) switch for low cost antibody detection. These sensing strategies have demonstrated their potential for resolving current challenges in antibody detection such as high selectivity, low operation cost, simple detection procedures, rapid detection, and low-fouling detection. This review provides a general update for recent developments in antibody detection strategies and potential solutions for future clinical point-of-care systems.

Comparison of self-assembled and micelle encapsulated QD chemosensor constructs for biological sensing

2015 ◽  
Vol 185 ◽  
pp. 249-266 ◽  
Author(s):  
Christopher M. Lemon ◽  
Daniel G. Nocera
Keyword(s):  
Self Assembly ◽  
Metabolic Profiling ◽  
Photophysical Properties ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Core Shell ◽  
Biological Sensing ◽  
Two Photon ◽  
Photon Excitation ◽  
Fret Efficiency

Whereas a variety of covalent conjugation strategies have been utilized to prepare quantum dot (QD)-based nanosensors, supramolecular approaches of self-assembly have been underexplored. A major advantage of self-assembly is the ability to circumvent laborious synthetic efforts attendant to covalent conjugation of a chemosensor to functionalized QDs. Here, we combine a CdSe/ZnS core–shell QD with gold(iii) corroles using both self-assembly and micelle encapsulation to form QD nanosensors. Appreciable spectral overlap between QD emission and corrole absorption results in efficient Förster resonance energy transfer (FRET), which may be initiated by one- or two-photon excitation. The triplet state of the gold(iii) corroles is quenched by molecular oxygen, enabling these constructs to function as optical O2 sensors, which is useful for the metabolic profiling of tumours. The photophysical properties, including QD and corrole lifetimes, FRET efficiency, and O2 sensitivity, have been determined for each construct. The relative merits of each conjugation strategy are assessed with regard to their implementation as sensors.

Spontaneous Self-Assembly, Functionalization, and Meso-Scale Host-Guest Science of Organic Nanotubes

2007 ◽  
Vol 1061 ◽  
Author(s):  
Naohiro Kameta ◽  
Mitsutoshi Masuda ◽  
Toshimi Shimizu
Keyword(s):  
Hollow Cylinder ◽  
Self Assembly ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Recognition System ◽  
Covalent Modification ◽  
Amino Groups ◽  
Guest Molecules ◽  
Inner Surface ◽  
Organic Nanotubes

ABSTRACTSelf-assembly in water at pH 6 and 10 of a wedge-shaped bolaamphiphile (1), bearing amino and glucose hydrophilic headgroups, was found to give two types of nanotubes with different inner diameters of 20 and 80 nm, respectively. X-ray diffraction and IR measurement revealed that the nanotubes have distinct inner and outer surfaces covered with amino and glucose headgroups, respectively. The nanotubes were able to effectively encapsulate an anionic spherical protein, ferritin, and DNA into the hollow cylinder under neutral pH conditions. Positively charged amino groups in the nanotube hollow cylinder proved to accelerate the encapsulation of the anionic biomacromolecules via electrostatic interaction. Covalent modification of the amino groups with a fluorescent donor dye on the inner surface of the nanotube allowed us to achieve the construction of an optical recognition system for encapsulation of guest molecules. Fluorescence resonance energy transfer (FRET) from the fluorescent donor located on the inner surface to the ferritin labeled with fluorescent acceptor enabled us to visualize the encapsulation and transportation features of the ferritin in the nanochannel shaped by the hollow cylinder structure.

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