Detection of Diarrheagenic Pathogens in Improved Water Using PCR-Based Specific Techniques in Low- and Middle-Income Countries: A Systematic Review and Meta-Analysis Protocol
Abstract Background: Effective application of Polymerase Chain Reaction (PCR) based methods in detecting pathogenic microorganisms in improved water samples were limited in low- and middle-income countries. This limitation initiated this systematic review to assess the effective characterization method that could be applied in low- and middle-income countries. Therefore, the aim of this review and analysis is to pullout the available evidences in characterization of pathogenic microorganisms in improved drinking water that could be effectively applied in low- and middle-income countries. Methods: We will search for published literatures using Google Scholar, PubMed or Medline, Scopus and Cochrane Library databases to explore the various PCR based methods recommended for the detection and enumeration of pathogens to examine quality of drinking water provided for communities. All studies conducted in low- and middle-income countries that use any types of PCR methods in water samples without publication date restriction were included in the review. The primary outcome of this study is assessing the magnitude of diarrheagenic pathogens detected in improved water supplies by PCR application in low- and middle-income countries. Critical appraisal will be conducted on all papers selected for inclusion in the review. The studies selected will be assessed by the reviewers for retrieval prior to inclusion in the review. Discussion: This review will systematically discover and integrate the evidence available on the detection of diarrheagenic pathogens through the application of PCR based methods for water quality determination. This facilitates water quality monitoring activities. In this review, information about the applied PCR method, detected diarrheagenic pathogens, water sample size, effectiveness in terms of time and cost, will be gathered and summarized. The findings from this review and analysis will provide compiled data on the magnitude of the detected diarrheagenic pathogenic microbes by type and amount in improved water supply through the application of PCR techniques in low- and middle-income countries. It is envisaged that the study will promote the application of PCR methods for drinking water quality monitoring in these low- and middle-income countries.