scholarly journals The Abnormal Distribution of Peripheral B1 Cells and Transition B Cells in Patients with Idiopathic Dilated Cardiomyopathy

2021 ◽  
Author(s):  
QUAN TANG ◽  
ZHIHONG CEN ◽  
JING LU ◽  
JINGWEI DONG ◽  
LIN QIN ◽  
...  
Keyword(s):  
B Cells ◽  
Dilated Cardiomyopathy ◽  
B Cell ◽  
Left Ventricular ◽  
Idiopathic Dilated Cardiomyopathy ◽  
Left Ventricular Ejection ◽  
Color Flow ◽  
Ventricular Ejection Fraction ◽  
B Cell Subsets ◽  
B1 Cells

Abstract Background The aberrant distribution of peripheral B cell subsets is associated with the pathogenesis of a variety of inflammatory and autoimmune diseases. However, the distribution of peripheral B cell subsets in patients with idiopathic dilated cardiomyopathy (DCM) has yet to elucidated. Methods Twenty-seven idiopathic DCM patients (DCM group), 18 heart failure controls (HF group) and 21 healthy individuals (HC group) were included in this study. Peripheral B cell subsets were analyzed by multi-color flow cytometry. Level of plasma β1 cholinergic receptor (β1-AR) autoantibody was assayed by ELISA. Additionally, clinical features were also collected. Results Compared with HF and HC groups, the percentage of B1 cells were significantly decreased, whereas the percentage of transitional B cells (Tr) were significantly increased in DCM group. Notably, the percentage of B1 cells was significantly lower in patients with β1-AR autoantibody positive DCM compared with β1-AR autoantibody negative patients. Correlation analysis showed that the percentage of B1 cells was negatively correlated with N-terminal pro-brain natriuretic peptide (NT-proBNP) and positively correlated with left ventricular ejection fraction (LVEF) in patients with DCM.Conclusions The study showed that B1 cells in peripheral blood of patients with idiopathic DCM were abnormally decreased, especially in those β1-AR autoantibody positive patients, while Tr cells are significantly increased, indicating that B1 cells and Tr cells may be implicated in the pathogenesis of idiopathic DCM.

Patterns of desmin expression in idiopathic dilated cardiomyopathy are related to the desmin mRNA and ubiquitin expression

2018 ◽  
Vol 67 (1) ◽  
pp. 11-19
Author(s):  
Agnieszka Pawlak ◽  
Emilia Rejmak-Kozicka ◽  
Katarzyna Elżbieta Gil ◽  
Andrzej Ziemba ◽  
Leszek Kaczmarek ◽  
...  
Keyword(s):  
Dilated Cardiomyopathy ◽  
Mrna Expression ◽  
Messenger Rna ◽  
Ubiquitin Proteasome System ◽  
Left Ventricular ◽  
Idiopathic Dilated Cardiomyopathy ◽  
Left Ventricular Ejection ◽  
Ventricular Ejection Fraction ◽  
Cell Remodeling ◽  
End Stage

Desmin expression depends on desmin messenger RNA (mRNA) and ubiquitin proteasome system. This process is poorly understood in dilated cardiomyopathy. The aim of the study was to investigate whether changes of desmin mRNA and ubiquitin expression correlate with types of desmin expression in cardiomyocytes. Endomyocardial biopsy was performed in 60 patients (85% men, mean age 46±14 years) with heart failure (HF; left ventricular ejection fraction <45%). Desmin and ubiquitin expression were analysed in histological sections by immunohistochemistry and in Western blot. Desmin mRNA expression was determined by fluorescent in situ hybridization methods. In patients with weak/even desmin expression, weak/even expression of ubiquitin in the cytosol and low desmin mRNA expression in the cytosol and nuclei of cardiomyocytes were observed. Expression of ubiquitin and desmin mRNA increased along with the progression of desmin cytoskeleton remodeling. Desmin mRNA and ubiquitin were weakly expressed/absent in cardiomyocytes with low/lack of desmin expression. Variations in desmin mRNA, desmin and ubiquitin expression were associated with gradual changes in myocardial structure and clinical parameters. To conclude, changes in ubiquitin and desmin mRNA expression are related to patterns of desmin expression. An increase in the expression of ubiquitin and desmin mRNA may be a protective feature against unfavorable cell remodeling. This may reduce the adverse effects of cytoskeleton damage in the early stages of HF. Low/lack ubiquitin and/or desmin mRNA expression may be markers of end-stage HF.

Prognostic impact of multiple fragmented QRS on cardiac events in idiopathic dilated cardiomyopathy

EP Europace ◽  
2020 ◽  
Author(s):  
Kyohei Marume ◽  
Teruo Noguchi ◽  
Tsukasa Kamakura ◽  
Emi Tateishi ◽  
Yoshiaki Morita ◽  
...  
Keyword(s):  
Dilated Cardiomyopathy ◽  
Cox Regression ◽  
Left Ventricular ◽  
Idiopathic Dilated Cardiomyopathy ◽  
Left Ventricular Ejection ◽  
Prognostic Impact ◽  
Cardiac Events ◽  
Ventricular Ejection Fraction ◽  
Fragmented Qrs ◽  
Kaplan Meier

Abstract Aims  To evaluate the prognostic impact of fragmented QRS (fQRS) on idiopathic dilated cardiomyopathy (DCM). Methods and results  We conducted a prospective observational study of 290 consecutive patients with DCM (left ventricular ejection fraction ≤ 40%) and narrow QRS who underwent cardiac magnetic resonance. We defined fQRS as the presence of various RSR′ patterns in ≥2 contiguous leads representing the anterior (V1–V5), inferior (II, III, and aVF), or lateral (I, aVL, and V6) myocardial segments. Multiple fQRS was defined as the presence of fQRS in ≥2 myocardial segments. Patients were divided into three groups: no fQRS, single fQRS, or multiple fQRS. The primary endpoint was a composite of hard cardiac events consisting of heart failure death, sudden cardiac death (SCD), or aborted SCD. The secondary endpoints were all-cause death and arrhythmic event. During a median follow-up of 3.8 years (interquartile range, 1.8–6.2), 31 (11%) patients experienced hard cardiac events. Kaplan–Meier analysis showed that the rates of hard cardiac events and all-cause death were similar in the single-fQRS and no-fQRS groups and higher in the multiple-fQRS group (P = 0.004 and P = 0.017, respectively). Multivariable Cox regression identified that multiple fQRS is a significant predictor of hard cardiac events (hazard ratio, 2.23; 95% confidence interval, 1.07–4.62; P = 0.032). The multiple-fQRS group had the highest prevalence of a diffuse late gadolinium enhancement pattern (no fQRS, 21%; single fQRS, 22%; multiple fQRS, 39%; P &lt; 0.001). Conclusion  Multiple fQRS, but not single fQRS, is associated with future hard cardiac events in patients with DCM.

Microvascular distensibility in two different vascular beds in idiopathic dilated cardiomyopathy

1995 ◽  
Vol 269 (6) ◽  
pp. H1973-H1980 ◽  
Author(s):  
H. Wroblewski ◽  
T. Norgaard ◽  
S. Haunso ◽  
J. Kastrup
Keyword(s):  
Skeletal Muscle ◽  
Blood Flow ◽  
Regression Analysis ◽  
Dilated Cardiomyopathy ◽  
Left Ventricular ◽  
Idiopathic Dilated Cardiomyopathy ◽  
Left Ventricular Ejection ◽  
Ventricular Ejection Fraction ◽  
Control Subjects ◽  
Arterial Blood

The local isotope-washout technique allows discrimination between blood flow in skin and muscle. Arteriolar constriction, mediated by the sympathetic nervous system, is abolished in a papaverine-histamine-relaxed vascular bed. Microvascular distensibility of relaxed resistance vessels was measured in skeletal muscle and skin of the lower limb in patients with congestive heart failure (CHF) secondary to idiopathic dilated cardiomyopathy and in healthy subjects. Vascular transmural pressure was elevated 40 mmHg by head-up tilt and caused muscle blood flow to increase by 11 +/- 9% in 20 CHF patients compared with 44 +/- 20% in 11 control subjects (P < 0.0003). Also the increase in skin blood flow was significantly reduced: 31 +/- 18% in 42 CHF patients compared with 62 +/- 29% in 25 control subjects (P < 0.001). Regression analysis demonstrated a significant association between microvascular distensibility in skin and skeletal muscle tissue (P = 0.003, r = 0.51, n = 31). Structure of terminal arterioles was determined in skin biopsies, and structural microangiopathy was found in 32 of 42 CHF patients. Multiple regression analysis demonstrated the degree of microangiopathy to be the only parameter significantly associated with microvascular distensibility (P = 0.005, r = 0.42). (There was no association to NY Heart Association functional class, left ventricular ejection fraction, duration of CHF, age of subject, or mean arterial blood pressure.) We conclude that patients with idiopathic dilated cardiomyopathy have similar decreased microvascular distensibility in skeletal muscle and skin. Furthermore, structural alterations in terminal arterioles seem to be associated with decreased distensibility and increased stiffness of the cutaneous microvascular bed.

Abstract 15759: Arrhythmic Risk Stratification in Patients With Idiopathic Dilated Cardiomyopathy and No Conventional Indications for Implantable Cardiac Defibrillator

Circulation ◽  
2014 ◽  
Vol 130 (suppl_2) ◽  
Author(s):  
Marco Merlo ◽  
Fabrizio Pirozzi ◽  
Davide Stolfo ◽  
D’Angelo Gianluca ◽  
Marco Alonge ◽  
...  
Keyword(s):  
Risk Stratification ◽  
Dilated Cardiomyopathy ◽  
Previous History ◽  
Left Ventricular ◽  
Muscle Disease ◽  
Idiopathic Dilated Cardiomyopathy ◽  
Left Ventricular Ejection ◽  
Ventricular Ejection Fraction ◽  
History Of ◽  
Duration Of Disease

Background: Primary prevention of major ventricular arrhythmias (MVA) in patients with idiopathic dilated cardiomyopathy (IDCM) is primarily based on left ventricular ejection fraction (LVEF) assessment. Nonetheless, unexpected MVA still affect patients considered at low-risk (LR), attesting the limitation of the current risk stratification. We sought to identify the clinical predictors of MVA in patients with IDCM and no conventional indications for implantable cardioverter defibrillator (ICD). Methods: In this retrospective, case-control study, among 922 patients enrolled in the Heart Muscle Disease Registry of Trieste from 1988 to 2013, we analyzed 414 IDCM patients considered at LR according to the following criteria: LVEF≥36% and no previous episodes of MVA (sustained ventricular tachycardia/non-fatal ventricular fibrillation (SVT/VF), appropriate ICD intervention and sudden cardiac death (SD)). Data were recorded at the last available medical examination before the index event. Patients were optimally treated at the time of index evaluation (87% and 84% of ACE-inhibitors/sartans and beta-blockers, respectively). Results: Over a median follow-up of 43 (IQR 16-116) months, 45 patients (11 % of patients at LR; 31% of the all MVA in whole IDCM population) experienced MVA. The mean age at the time of event was 51±14 years. They were characterized by a previous history of syncope in 10 patients (22%), LVEF of 42±6% with a normal value (>50%) in 10 patients (22%), significant LV dilation (VTDi>90ml/m 2 ) in 16 patients (35%) and left bundle branch block 12 patients (27%). Independent risk factors for MVA in LR subgroup were previous history of syncope (OR 3.41, 1.45-8.03, p=0.004), larger left ventricular dilation (OR 2.62, 1.72-3.99, p<0.0001) and longer duration of disease (OR 1.39, 1.42-2.63, p<0.0001). Conclusions: in a large cohort of IDCM patients nearly one-third of the MVA occurred in a population apparently at LR of events. History of syncope, larger LV dilatation and significant duration of disease emerged as strong predictors of MVA and should be considered in the arrhythmic risk stratification of patients without conventional criteria for SD primary protection.

Sign in / Sign up

Export Citation Format

Share Document