scholarly journals The Diagnosis of Sheep Cystic Echinococcosis by Native Antigen B - ELISA Method

2021 ◽  
Author(s):  
Shahrokh Shirazi ◽  
Nasser Hoghooghi-Rad ◽  
Rasoul Madani
Keyword(s):  
Cystic Echinococcosis ◽  
Zoonotic Diseases ◽  
Economic Damage ◽  
Specific Detection ◽  
Serum Samples ◽  
Antigen B ◽  
Elisa Technique ◽  
Native Antigen ◽  
Livestock Productivity ◽  
East Azerbaijan

Abstract Cystic echinococcosis (CE) is one of the most prevalent zoonotic diseases in some countries in the world. Cystic echinococcosis is considered a neglected disease. This disease increases economic damage via medical costs and loss of human and livestock productivity. The aim of the current study gains a better understanding of the prevalence of CE in sheep. Totally 250 sheep sera were collected. 25 serum samples from newborn lambs were used as negative, and 25 others were obtained from slaughterhouses as positive to infection with CE and 200 unknown sera. Antigen B Isolated from hydatid cysts fluid was used for designing ELISA methods. The first Method used anti-Sheep conjugate (SIGMA, at 1:3000 dilution). According to the results, the seroprevalence of CE in East Azerbaijan of Iran was 5.5% and sensitivity and specificity for the diagnosis of hydatidosis in sheep by AgB-ELISA methods was 92%. Using Antigen B in ELISA design for hydatidosis diagnosis has attracted researchers in recent years. During this study, an Iranian native B antigen was used to design the specific detection of hydatidosis in sheep using a specific ELISA technique. The results have shown that using Antigen B in ELISA design is so valuable.

scholarly journals Comparison of the Utility of Recombinant B8/2 Subunit of the Antigen B, Native Antigen, and a Commercial ELISA Kit in the Diagnosis of Human Cystic Echinococcosis

2019 ◽  
Vol 23 (4) ◽  
pp. 246-252
Author(s):  
Amir Savardashtaki ◽  
Zohreh Mostafavi-Pour ◽  
Farzaneh Arianfar ◽  
Bahador Sarkari ◽  
◽  
...  
Keyword(s):  
Cystic Echinococcosis ◽  
Elisa Kit ◽  
Antigen B ◽  
Native Antigen ◽  
Human Cystic Echinococcosis

scholarly journals Seroepidemiological Study of Hydatid Cyst Using AgB by ELISA in Patients Admitted to Central Laboratory of Baqiyatallah Hospital

2019 ◽  
Vol 7 (1) ◽  
pp. 15-18
Author(s):  
Manuochehr Esmaali Ghouraneh ◽  
Mohsen Saberi ◽  
Sohrab Farhadineko ◽  
Kazem Ahmadi ◽  
Rezvan Yousefi ◽  
...  
Keyword(s):  
Hydatid Cyst ◽  
Urban Communities ◽  
Positive Response ◽  
Academic Education ◽  
Central Laboratory ◽  
Serum Samples ◽  
Antigen B ◽  
Middle East Countries ◽  
Elisa Technique ◽  
Hydatid Cyst Fluid

Background: One of the most important parasitic diseases in human is cystic echinococcosis (CE) which is caused by the larval stage of Echinococcus granulosus. The disease is a health problem in the Middle East countries such as Iran. Objective: The aim of this study was seroepidemiological evaluation of human CE using antigen B by ELISA technique in patients referred to Baqiyatallah Hospital, Tehran, Iran. Materials and Methods: A total of 909 serum samples were randomly prepared in the central laboratory. Antigen B was extracted from sheep hydatid cyst fluid (HCF). All serum samples were evaluated by indirect ELISA and the suspected cases were rechecked. Demographic characteristics were collected by a questionnaire and cut-off was calculated as X±2SD. Results: In the present study, 0.8% of the collected samples were seropositive by ELISA technique. Of the 909 participants, 498 (with 1% positive response) and 411 (with 0.49% positive response) were female and male, respectively. The majority of the cases were the employee and retired military personnel (with 1.05% positive response). The most frequent age ranges were 50-59 and 70-79 with 28.57% positive response in each and in participants with academic education (85.72%). There was no statistically significant association between hydatid cyst and variables evaluated in this study. Conclusion: Our results showed a low seroprevalence of human CE. It may be related to the lifestyle of people following the improvement in public health specially in urban communities.

Effects of X-Ray Exposure on Some CMI Regulatory Cytokines in Technicians Serum Samples

2018 ◽  
Vol 9 (4) ◽  
Author(s):  
Weam S Al-Hamadany
Keyword(s):  
Clinical Status ◽  
Cell Mediated Immunity ◽  
Normal Range ◽  
Serum Samples ◽  
X Ray ◽  
Immunosuppressive Factor ◽  
Elisa Technique ◽  
Immunological Process ◽  
Field Workers ◽  
Tomography Scan

Ionizing radiation considered as an immunosuppressive factor upon over dose of exposure. Radiation field workers usually following a periodic checkup to monitor changes in their clinical status. Cell Mediated Immunity (CMI) has an important cytokines that regulate this specific immunological process. This study estimated the Interleukins (IL-2, IL-12 and IL-18) levels in serum samples using ELISA technique. Serum samples were collected from X-ray Technicians (Radiography, Fluoroscopy and Computed Tomography Scan Technicians) working in AL-Muthanna governorate hospitals. A total of (60) technicians and (30) control were involved in this research. Results showed significant decrease in IL-2 levels and increase in IL-18 levels in test groups (technicians) as compared with controls. While, IL-12 levels did not show a difference; all obtained values were within normal range. Overdose of X-ray exposure caused CMI suppression via disturbing the levels of critical cytokines (IL-2 and IL-18) leading to CMI loss regulation.

scholarly journals Some observations on the sero-prevalence of heartwater and tick infestation in Zambian goats : research communication

2004 ◽  
Vol 71 (2) ◽  
Author(s):  
B. Ahmadu ◽  
C.E.A. Lovelace ◽  
S. Mahan
Keyword(s):  
Tick Species ◽  
Tick Infestation ◽  
Amblyomma Variegatum ◽  
Arid Areas ◽  
Serum Samples ◽  
Ehrlichia Ruminantium ◽  
Antigen Elisa ◽  
Elisa Technique ◽  
Species Being ◽  
Semi Arid

A survey was carried out to define the distribution of heartwater in goats that originated from six districts in communal grazing semi-arid areas of Zambia. A total of 181 samples (40.1 %) out of 451 serum samples from adult goats were positive for Ehrlichia ruminantium antibodies after screening using indirect MAP-1B antigen ELISA technique with statistically significant differences (P < 0.01) between the six districts. Out of 1 036 adult goats examined for tick infestation, 105 were infested by ticks, with Amblyomma species being the most dominant tick encountered. Amblyomma variegatum, which is the vector for heartwater transmission in Zambia constituted 42.4 % of the tick species, identified. The overall tick infestation rate was 10 % while the tick:goat ratio was 2.1:1. Amblyomma variegatum appears to be widespread throughout the study area, as are antibodies to E. ruminantium.

scholarly journals A T-cell diagnostic test for cystic echinococcosis based on Antigen B peptides

2017 ◽  
Vol 39 (12) ◽  
pp. e12499 ◽  
Author(s):  
L. Petrone ◽  
V. Vanini ◽  
M. Amicosante ◽  
A. Corpolongo ◽  
M. A. Gomez Morales ◽  
...  
Keyword(s):  
T Cell ◽  
Diagnostic Test ◽  
Cystic Echinococcosis ◽  
Antigen B

scholarly journals Comparison of serological and clinical findings in Turkish patients with cystic echinococcosis

2006 ◽  
Vol 43 (4) ◽  
pp. 220-225 ◽  
Author(s):  
A. Yolasigmaz ◽  
K. Reiterová ◽  
M. Turk ◽  
E. Reyhan ◽  
A. Bozdag ◽  
...  
Keyword(s):  
Western Blot ◽  
Rural Areas ◽  
Cystic Echinococcosis ◽  
Correct Diagnosis ◽  
Clinical Findings ◽  
Serum Samples ◽  
First Case ◽  
Long Time ◽  
Back Ache ◽  
The Individual

AbstractCystic echinococcosis (CE), caused by the cestode Echinococcus granulosus, is potentially dangerous for humans. The aim of this study was to examine serological and clinical findings regarding cysts localisation and individual responses in 54 patients with CE. The majority of patients in this study were females (63 %) and the average age was 46.3 years. Most of the patients lived in rural areas or kept a dog (46 %) for a long time. The most frequent symptoms were hypochondrial pain (48.9 %), epigastrial discomfort (27.7 %), vomiting (21.3 %), minor cough (12.8 %), urticaria (6.3 %), weakness (4.3 %), fever (2.1 %), side-or back-ache (4.3 %). However, 17 % of the patients showed no symptoms. In every case, the ultrasound (USG) and/or computer tomography (CT) investigations were positive. In most cases (53.2 % of the patients) a single cyst was found but 46.8 % of the patients had multiple cyst formations (from 2 to 9 cysts) located in the liver. Sporadic lung, splenetic, mesenterial, tibial and cerebral localisations were also found. The patients were individually treated with albendazol (10–15 mg/kg) five days prior and six months after the surgical treatment. Serum samples were investigated by the serological techniques: IHAT, ELISA and Western blot using hydatid fluid antigen. In the patient sera, the specific antibody levels were mostly increased after surgery. Different results were obtained only in two patients. In the first case, seroconversion was delayed. In the other case all ELISA results were negative, however, the Western blot analysis and surgery proved the presence of CE. The results suggest that the different antibody response of patients depends on the individual immune response. Multiple localization and various stages of CE cysts demonstrate the necessity of a complex approach for the confirmation of a correct diagnosis.

Sign in / Sign up

Export Citation Format

Share Document