scholarly journals The Preventive Effect of Lactobacillus Fermentum SCHY34 Isolated from Traditional Fermented Yak Yogurt on Lead Acetate-Induced Neurological Damage in SD Rats

2021 ◽  
Author(s):  
Xingyao Long ◽  
Haibo Wu ◽  
Yujing Zhou ◽  
Yunxiao Wan ◽  
Xunmei Kan ◽  
...  
Keyword(s):  
Heavy Metals ◽  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Brain Tissue ◽  
Lead Poisoning ◽  
Lead Acetate ◽  
Aminolevulinic Acid ◽  
Cognitive Capacity ◽  
Acetate Solution ◽  
Sd Rats

Abstract Background: Lead is a heavy metal that is widespread in nature and has extremely strong chemical toxicity in the human body especially harmful to the human nervous system. Lactic acid bacteria (LAB) can be used to maintain the balance of the intestinal microbiota, and recent reports have shown that LAB can remove metal ions through adsorption. In this experiment, Sprague-Dawley (SD) rats were treated with 200 mg/L of lead acetate solution daily to induce chronic lead poisoning, and oral LF-SCHY34 to study its mitigation effects and mechanisms on rat neurotoxicity.Results: Through electron microscopy and energy spectrum analysis, it was found that the surface of the lactic acid bacteria adsorbed a large amount of lead ions, and the O and N elements in the bacteria were significantly reduced. Animal experiments showed that LF-SCHY34 maintained the morphology of rat liver, kidney, and hippocampi, reduced the accumulation of lead in the blood, liver, kidney, and brain tissue, reduced the activity of alanine aminotransferase (ALT), aspartate aminotransferase (AST), creatinine (CRE), and blood urea nitrogen (BUN) in rat serum, and increased δ-aminolevulinic acid dehydratase (δ-ALAD) activity in serum. Further, LF-SCHY34 alleviated the lead-induced decline in spatial memory and response capacity of SD rats, and also regulated the secretion of neurotransmitters and related enzyme activities in the brain tissue of rats. LF-SCHY34 inhibited the secretion of glutamate and the activity of monoamine oxidase in brain tissue, promoted the synthesis of glutamine (Glu), norepinephrine (NE), and cyclic adenosine monophosphate (cAMP), and increased the activities of glutamine synthetase (GS), acetylcholinesterase (AchE), and adenylate cyclase (AC). In addition, the expression of genes related to cognitive capacity in rat brain tissues such as calmodulin (CaM), protein kinase A (PKA), brain-derived neurotrophic factor (BDNF), c-fos, c-jun was increased by LF-SCHY as was the expression of the antioxidation genes and the anti-apoptotic gene. Conclusions: Compared with the lead poisoning treatment drug EDTA, LF-SCHY34 not only had greater lead discharge capacity than EDTA, but also had a greater alleviating effect on organ damage and oxidative damage caused by lead. As a food-grade LAB, LF-SCHY34 has great potential and research value for removing heavy metals from food and alleviating the toxicity of heavy metals.

Biosorption of heavy metals by lactic acid bacteria and identification of mercury binding protein

2013 ◽  
Vol 164 (7) ◽  
pp. 701-709 ◽  
Author(s):  
Hideki Kinoshita ◽  
Yui Sohma ◽  
Fumika Ohtake ◽  
Mitsuharu Ishida ◽  
Yasushi Kawai ◽  
...  
Keyword(s):  
Heavy Metals ◽  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Binding Protein ◽  
Biosorption Of Heavy Metals

Application of lactic acid bacteria in removing heavy metals and aflatoxin B1 from contaminated water

2016 ◽  
Vol 74 (3) ◽  
pp. 625-638 ◽  
Author(s):  
Rafaat M. Elsanhoty ◽  
I. A. Al-Turki ◽  
Mohamed Fawzy Ramadan
Keyword(s):  
Heavy Metals ◽  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Aflatoxin B1 ◽  
Lactobacillus Acidophilus ◽  
Contact Time ◽  
World Health ◽  
Probiotic Bacteria ◽  
Contaminated Water ◽  
Bacterial Concentration

In this study selected lactic acid bacteria (LAB, Lactobacillus acidophilus, Lactobacillus rhamnosus, Lactobacillus plantrium and Streptococcus thermophiles) and probiotic bacteria (Bifidobacterium angulatum) were tested for their ability in removing heavy metals (HM) including cadmium (Cd), lead (Pb) and arsenic (As) as well as aflatoxin B1 (AFB1) from contaminated water. The biosorption parameters (pH, bacterial concentration, contact time and temperature) of removal using individual as well as mixed LAB and probiotic bacteria were studied. Removal of HM and AFB1 depended on the strain, wherein the process was strongly pH-dependent with high removal ability at a pH close to neutral. The increase in bacterial concentration enhanced the removal of Cd, Pb and As. Also, increasing of contact time and temperature increased the ability of LAB to remove HM. The effect of contact time on Cd removal was slightly different when freshly cultured cells were used. The removal of Cd, Pb and As decreased with the increase in the initial metal concentration. The most effective HM removers were Lactobacillus acidophilus and Bifidobacterium angulatum. The system was found to be adequate for concentrations of HM under investigation. At the end of the operation, the concentration of HM reached the level allowed by the World Health Organization regulations.

scholarly journals STRUCTURAL-FUNCTIONAL REORGANIZATION OF SKIN AND ITS DERIVATIVES UNDER EXPERIMENTAL SUBACUTE OF INTOXICATION BY HEAVY METALS

2019 ◽  
Vol 96 (6) ◽  
pp. 593-596
Author(s):  
Tatyana V. Nikolaeva ◽  
V. S. Polyakova ◽  
N. P. Setko ◽  
L. G. Voronina
Keyword(s):  
Heavy Metals ◽  
Proliferative Activity ◽  
Lead Acetate ◽  
Dermal Papilla ◽  
Hair Follicles ◽  
The Body ◽  
Acetate Solution ◽  
Sodium Dichromate ◽  
Ki 67 ◽  
Nickel Chromium

There was executed an experimental study of the effect of salts of heavy metals (nickel, chromium, lead and zinc) entering the body by peroral route, on the morphology of the skin and its derivatives (hair follicles and sebaceous glands). The experiment was performed on C57BL / 6 mice with the use of the induction of hair follicle cycle by depilation. Under the subacute intoxication with salts of nickel, chromium and lead, there were revealed such signs of a dystrophic anagen as ectopia of granules of melanin in the dermal papilla and perifollicular tissue, enlarged channels of the hair. The duration of the anagen stage if compared with the control did not change. Under the intoxication with salts of nickel and lead there was revealed infiltration by mononuclear dermis and hypodermis. Lead acetate gave rise in the capillary congestion of the dermis, followed by diapedesis of erythrocytes and infiltration of the dermis by siderophages. In the course of the immunohistochemical study of the proliferative activity of keratinocytes of the skin integument derivatives with the use of antibodies to Ki-67, there was revealed a significant increase of proliferative activity of keratinocytes in comparison with the control under the use of a solution of zinc sulphate and sodium dichromate and its decrease with the use of lead acetate solution.

scholarly journals Evaluation and Proteomic Analysis of Lead Adsorption by Lactic Acid Bacteria

2019 ◽  
Vol 20 (22) ◽  
pp. 5540 ◽  
Author(s):  
Liu ◽  
Zheng ◽  
Ma ◽  
Sarwar ◽  
Zhao ◽  
...  
Keyword(s):  
Heavy Metals ◽  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Absolute Quantitation ◽  
Lead Adsorption ◽  
Removal Of Heavy Metals ◽  
Itraq Proteomics ◽  
Rate Of Absorption ◽  
Isobaric Tags ◽  
Substance Transport

Heavy metals are a growing threat to human health due to the resulting damage to the ecology; the removal of heavy metals by lactic acid bacteria (LAB) has been a focus of many studies. In this study, 10 LAB strains were evaluated for their ability to absorb and tolerate lead. Lactobacillus plantarum YW11 was found to possess the strongest ability of lead absorbing and tolerance, with the rate of absorption as high as 99.9% and the minimum inhibitory concentration of lead on YW11 higher than 1000 mg/L. Based on the isobaric tags for relative and absolute quantitation (iTRAQ) proteomics analysis of YW11, a total of 2009 proteins were identified both in the lead-treated strain and the control without the lead treatment. Among these proteins, 44 different proteins were identified. The abundance of 25 proteins increased significantly, and 19 proteins decreased significantly in the treatment group. These significantly differential abundant proteins are involved in the biological processes of amino acid and lipid metabolism, energy metabolism, cell wall biosynthesis, and substance transport. This study contributed further understanding of the molecular mechanism of L. plantarum in the binding and removal of lead to explore its potential application in counteracting heavy metal pollution of environment, food, and other fields.

Lactic acid bacteria and yeasts in kefir grains and kefir made from them

2002 ◽  
Vol 28 (1) ◽  
pp. 1-6 ◽  
Author(s):  
E Simova ◽  
D Beshkova ◽  
A Angelov ◽  
Ts Hristozova ◽  
G Frengova ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Kefir Grains
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