scholarly journals First Isolation and Molecular Characterization of Pseudorabies Virus Detected in Turkey

2021 ◽  
Author(s):  
Gizem Aytoğu ◽  
Eda B. Toker ◽  
Ozkan Yavas ◽  
Berfin Kadiroglu ◽  
Ozer Ates ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Pseudorabies Virus ◽  
Clinical Signs ◽  
Dna Amplification ◽  
Vero Cells ◽  
Virus Genome ◽  
Buffy Coat ◽  
Fresh Tissue ◽  
Tissue Samples

Abstract Pigs are the main host species for the pseudorabies virus, while it causes fatal encephalitis in many species including human being. The objective of this article is to report the first clinical case of pseudorabies as well as isolation and molecular characterization of the virus from a hunting dog in Bursa province, Turkey. The dog showing clinical signs including pruritus and neurological signs as stumbling and inability to stand up compatible with pseudorabies. The virus isolate was obtained from supernatant of fresh tissue samples from cerebellum, cornu ammonis, spleen, salivary gland, and conjunctival swab, as well as serum, and buffy coat samples. The glycoprotein C region is targeted for viral DNA amplification. Pseudorabies virus genome was detected both in fresh tissues and supernatants of 3rd passage on Vero cells. Number of PCR positive samples were dramatically increased after having cell culture inoculations. Genome sequencing revealed that the strain (Bursa-10303) isolated in Turkey where a non-endemic area is for the virus is dropped into clade A. This study confirms the presence of psedorabies infection in the wild life reservoirs in Turkey. Future studies may clarify the importance of the infection for Turkey where there is no common pig production.

scholarly journals Recovery and Characterization of a Chimeric Rinderpest Virus with the Glycoproteins of Peste-des-Petits-Ruminants Virus: Homologous F and H Proteins Are Required for Virus Viability

2000 ◽  
Vol 74 (19) ◽  
pp. 9039-9047 ◽  
Author(s):  
Subash C. Das ◽  
Michael D. Baron ◽  
Thomas Barrett
Keyword(s):  
Clinical Signs ◽  
Vero Cells ◽  
Virus Genome ◽  
Peste Des Petits Ruminants ◽  
Live Virus ◽  
Domestic Ruminants ◽  
Rescue System ◽  
Ppr Virus ◽  
Rescue Procedure

ABSTRACT Rinderpest (RP) and peste-des-petits-ruminants (PPR) are two important diseases of domestic ruminants. To improve on currently available vaccines against PPR, we have created cDNA copies of the RP virus genome in which either the fusion (F) or hemagglutinin (H) gene, or both, was replaced with the corresponding gene from PPR virus. It was necessary to develop a modified rescue system in which the T7 RNA polymerase was provided by a recombinant fowlpox virus and the entire rescue procedure took place in Vero cells before we could obtain live virus from these chimeric constructs. No virus was recovered when only one of the glycoprotein genes was changed, but a chimeric virus containing both F and H genes from PPR virus was reproducibly rescued from cDNA, indicating that a virus-specific functional interaction takes place between the F and H proteins. The rescued virus expressing the PPR glycoproteins grew more slowly in tissue culture than either parental virus and formed abnormally large syncytia. Goats infected with the chimera showed no adverse reaction, as assessed by clinical signs, temperature, leukocyte count, virus isolation, and serology, and were protected from subsequent challenge with wild-type PPR virus.

scholarly journals Molecular characterization and distribution of Plasmodium matutinum, a common avian malaria parasite

Parasitology ◽  
2017 ◽  
Vol 144 (13) ◽  
pp. 1726-1735 ◽  
Author(s):  
GEDIMINAS VALKIŪNAS ◽  
MIKAS ILGŪNAS ◽  
DOVILĖ BUKAUSKAITĖ ◽  
VAIDAS PALINAUSKAS ◽  
RASA BERNOTIENĖ ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Molecular Characterization ◽  
Malaria Parasite ◽  
Avian Malaria ◽  
Clinical Signs ◽  
Molecular Data ◽  
Infected Blood ◽  
Avian Malaria Parasite ◽  
Thrush Nightingale

SUMMARYSpecies of Plasmodium (Plasmodiidae, Haemosporida) are widespread and cause malaria, which can be severe in avian hosts. Molecular markers are essential to detect and identify parasites, but still absent for many avian malaria and related haemosporidian species. Here, we provide first molecular characterization of Plasmodium matutinum, a common agent of avian malaria. This parasite was isolated from a naturally infected thrush nightingale Luscinia luscinia (Muscicapidae). Fragments of mitochondrial, apicoplast and nuclear genomes were obtained. Domestic canaries Serinus canaria were susceptible after inoculation of infected blood, and the long-lasting light parasitemia developed in two exposed birds. Clinical signs of illness were not reported. Illustrations of blood stages of P. matutinum (pLINN1) are given, and phylogenetic analysis identified the closely related avian Plasmodium species. The phylogeny based on partial cytochrome b (cyt b) sequences suggests that this parasite is most closely related to Plasmodium tejerai (cyt b lineage pSPMAG01), a common malaria parasite of American birds. Both these parasites belong to subgenus Haemamoeba, and their blood stages are similar morphologically, particularly due to marked vacuolization of the cytoplasm in growing erythrocytic meronts. Molecular data show that transmission of P. matutinum (pLINN1) occurs broadly in the Holarctic, and the parasite likely is of cosmopolitan distribution. Passeriform birds and Culex mosquitoes are common hosts. This study provides first molecular markers for detection of P. matutinum.

scholarly journals Epidemiology, clinical signs, histopathology and molecular characterization of canine leproid granuloma: a retrospective study of cases from Brazil

2011 ◽  
Vol 22 (3) ◽  
pp. 249-256 ◽  
Author(s):  
Lissandro Gonçalves Conceição ◽  
Livia Maria Rosa Acha ◽  
Alexandre Secorun Borges ◽  
Fernanda G. Assis ◽  
Fabricia Hallack Loures ◽  
...  
Keyword(s):  
Retrospective Study ◽  
Molecular Characterization ◽  
Clinical Signs

scholarly journals Isolation and Molecular Characterization of Aspergillus fumigatus and Aspergillus flavus Isolated from Poultry Birds in Ado- Ekiti, Nigeria

2020 ◽  
pp. 31-44
Author(s):  
E. D. Fagbohun ◽  
K. J. Ayantola ◽  
A. J. Toyin-Famoroti
Keyword(s):  
Aspergillus Fumigatus ◽  
Molecular Characterization ◽  
Aspergillus Flavus ◽  
Clinical Signs ◽  
Signs And Symptoms ◽  
Aspergillus Species ◽  
Exercise Intolerance ◽  
State University ◽  
Poultry Farms

Aim: The study was carried out to isolate and identify Aspergillus species from commercial birds with suspected aspergillosis in the poultry farms within Ado Ekiti metropolis Nigeria.  Place and Period of Study: The study was carried out in the Department of Microbiology, Faculty of Science, Ekiti State University Ado Ekiti, Nigeria in August 2016. Methodology: A total of 35 sick/suspected birds were collected randomly from three poultry farms. At Ago-Aduloju poultry farms, 15 samples were randomly collected from 1000 birds while at Ekiti State University poultry farms, 10 samples were randomly collected from 500 birds. At Federal Polytechnic Ado Ekiti poultry farms, 10 samples were randomly collected from 700 birds. The bird’s selection was on the basis of clinical signs and symptoms such as difficulty in breathing, weight loss, drooping of wings and exercise intolerance. Swab samples were collected from each suspected/sick bird for mycological culture and molecular characterization of the isolates from each bird was carried out. The isolates were identified based on the color of the culture on Potato Dextrose Agar and microscopic examination. Molecular identification was done using 23S Ribosomal RNA Gene and Partial Sequence. Results: Six fungal strains that showed similar morphological and cultural characteristics of Aspergillus species were isolated. The isolates were coded ASP 1, ASP 2, ASP 3, ASP 4, ASP 5, and ASP 6. The identified organisms were; Aspergillus fumigatus qH 107 (ASP 1), Aspergillus fumigatus qH 107 (ASP 2), Aspergillus flavus M09 (ASP 3), Aspergillus flavus UOMS6 (ASP 4), Aspergillus fumigatus qH 107 (ASP 5), Aspergillus flavus qH 107 (ASP 6). Conclusion: It is evident that Aspergillus species were predominant in poultry farms selected in this study. Necessary precaution should be put in place to prevent the spread of aspergillosis. Poultry farmers are advised to avoid damp environments, moldy feeds, dry and dusty litters. Adequate ventilation should always be provided in poultry farms to prevent Aspergillosis.

scholarly journals Molecular Characterization of Chelonid Alphaherpesvirus 5 in a Black Turtle (Chelonia mydas) Fibropapilloma from Baja California Sur, Mexico

Animals ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 105
Author(s):  
Eduardo Reséndiz ◽  
Helena Fernández-Sanz ◽  
José Francisco Domínguez-Contreras ◽  
Amelly Hyldaí Ramos-Díaz ◽  
Agnese Mancini ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Molecular Analysis ◽  
Baja California ◽  
Clinical Signs ◽  
Chelonia Mydas ◽  
Baja California Sur ◽  
Epidermal Hyperplasia ◽  
Routine Monitoring ◽  
Hematological Values

During routine monitoring in Ojo de Liebre Lagoon, Mexico, a juvenile black turtle (Chelonia mydas) was captured, physically examined, measured, weighed, sampled, and tagged. The turtle showed no clinical signs suggestive of disease. Eleven months later, this turtle was recaptured in the same area, during which one lesion suggestive of fibropapilloma on the neck was identified and sampled for histopathology and molecular analysis. Histopathology revealed hyperkeratosis, epidermal hyperplasia, acanthosis, papillary differentiation and ballooning degeneration of epidermal cells, increased fibroblasts in the dermis, and angiogenesis, among other things. Hematological values were similar to those reported for clinically healthy black turtles and did not show notable changes between the first capture and the recapture; likewise, clinicopathological evaluation did not show structural or functional damage in the turtle’s systems. The chelonid alphaherpesvirus 5 (ChHV5) UL30 gene was amplified and sequenced for phylogeny; Bayesian reconstruction showed a high alignment with the genus Scutavirus of the Eastern Pacific group. This is one of the first reports of ChHV5 in a cutaneous fibropapilloma of a black turtle in the Baja California peninsula.

scholarly journals Outbreak of organophosphorus compound-induced delayed neurotoxicity in water buffaloes

2021 ◽  
Vol 51 (10) ◽  
Author(s):  
Igor Ribeiro dos Santos ◽  
Luan Cleber Henker ◽  
Tainah Pereira Dal Pont ◽  
Welden Panziera ◽  
Saulo Petinatti Pavarini ◽  
...  
Keyword(s):  
Cholinesterase Activity ◽  
Organophosphorus Compounds ◽  
Clinical Signs ◽  
Pathological Examination ◽  
Serum Cholinesterase ◽  
Fresh Tissue ◽  
Tissue Samples ◽  
Water Buffaloes ◽  
Delayed Neurotoxicity ◽  
Layer Chromatography

ABSTRACT: Forty 1-2-y-old water buffaloes were simultaneously treated with trichlorfon and chlorpyrifos products in the recommended dose for cattle. After a week, 19 animals started presenting clinical signs characterized by apathy, diarrhea, aggressiveness, dehydration, and motor incoordination, followed by flaccid paralysis and permanent lateral recumbency. All affected buffaloes died after a clinical course of 1-4 days. Reduction of serum cholinesterase activity in three cases was indicative of significant exposure to organophosphorus compounds (OPs). Pathological examination of three buffaloes revealed no gross and histological lesions. By thin layer chromatography, chlorpyrifos residues and trace of trichlorfon residues were detected in fresh tissue samples. The epidemiological, clinical, pathological, and toxicological findings were highly compatible with OPs-induced delayed neurotoxicity, a neurological manifestation rarely described in domestic animals.

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