Molecular Characterization of Chelonid Alphaherpesvirus 5 in a Black Turtle (Chelonia mydas) Fibropapilloma from Baja California Sur, Mexico

During routine monitoring in Ojo de Liebre Lagoon, Mexico, a juvenile black turtle (Chelonia mydas) was captured, physically examined, measured, weighed, sampled, and tagged. The turtle showed no clinical signs suggestive of disease. Eleven months later, this turtle was recaptured in the same area, during which one lesion suggestive of fibropapilloma on the neck was identified and sampled for histopathology and molecular analysis. Histopathology revealed hyperkeratosis, epidermal hyperplasia, acanthosis, papillary differentiation and ballooning degeneration of epidermal cells, increased fibroblasts in the dermis, and angiogenesis, among other things. Hematological values were similar to those reported for clinically healthy black turtles and did not show notable changes between the first capture and the recapture; likewise, clinicopathological evaluation did not show structural or functional damage in the turtle’s systems. The chelonid alphaherpesvirus 5 (ChHV5) UL30 gene was amplified and sequenced for phylogeny; Bayesian reconstruction showed a high alignment with the genus Scutavirus of the Eastern Pacific group. This is one of the first reports of ChHV5 in a cutaneous fibropapilloma of a black turtle in the Baja California peninsula.

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Molecular characterization of Prunus necrotic ringspot virus isolated from rose in Brazil

Ciência Rural ◽

10.1590/0103-8478cr20141810 ◽

2015 ◽

Vol 45 (12) ◽

pp. 2197-2200 ◽

Cited By ~ 2

Author(s):

Thor Vinícius Martins Fajardo ◽

Monique Bezerra Nascimento ◽

Marcelo Eiras ◽

Osmar Nickel ◽

Gilvan Pio-Ribeiro

Keyword(s):

Amino Acid ◽

Molecular Characterization ◽

Molecular Analysis ◽

Nucleotide Sequences ◽

Amino Acid Sequences ◽

Causal Agent ◽

Ringspot Virus ◽

Prunus Necrotic Ringspot Virus ◽

First Time

ABSTRACT: There is no molecular characterization of Brazilian isolates of Prunus necrotic ringspot virus (PNRSV), except for those infecting peach. In this research, the causal agent of rose mosaic was determined and the movement (MP) and coat (CP) protein genes of a PNRSV isolate from rose were molecularly characterized for the first time in Brazil. The nucleotide and deduced amino acid sequences of MP and CP complete genes were aligned and compared with other isolates. Molecular analysis of the MP and CP nucleotide sequences of a Brazilian PNRSV isolate from rose and others from this same host showed highest identities of 96.7% and 98.6%, respectively, and Rose-Br isolate was classified in PV32 group.

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Molecular characterization and distribution of Plasmodium matutinum, a common avian malaria parasite

Parasitology ◽

10.1017/s0031182017000737 ◽

2017 ◽

Vol 144 (13) ◽

pp. 1726-1735 ◽

Cited By ~ 13

Author(s):

GEDIMINAS VALKIŪNAS ◽

MIKAS ILGŪNAS ◽

DOVILĖ BUKAUSKAITĖ ◽

VAIDAS PALINAUSKAS ◽

RASA BERNOTIENĖ ◽

...

Keyword(s):

Molecular Markers ◽

Molecular Characterization ◽

Malaria Parasite ◽

Avian Malaria ◽

Clinical Signs ◽

Molecular Data ◽

Infected Blood ◽

Avian Malaria Parasite ◽

Thrush Nightingale

SUMMARYSpecies of Plasmodium (Plasmodiidae, Haemosporida) are widespread and cause malaria, which can be severe in avian hosts. Molecular markers are essential to detect and identify parasites, but still absent for many avian malaria and related haemosporidian species. Here, we provide first molecular characterization of Plasmodium matutinum, a common agent of avian malaria. This parasite was isolated from a naturally infected thrush nightingale Luscinia luscinia (Muscicapidae). Fragments of mitochondrial, apicoplast and nuclear genomes were obtained. Domestic canaries Serinus canaria were susceptible after inoculation of infected blood, and the long-lasting light parasitemia developed in two exposed birds. Clinical signs of illness were not reported. Illustrations of blood stages of P. matutinum (pLINN1) are given, and phylogenetic analysis identified the closely related avian Plasmodium species. The phylogeny based on partial cytochrome b (cyt b) sequences suggests that this parasite is most closely related to Plasmodium tejerai (cyt b lineage pSPMAG01), a common malaria parasite of American birds. Both these parasites belong to subgenus Haemamoeba, and their blood stages are similar morphologically, particularly due to marked vacuolization of the cytoplasm in growing erythrocytic meronts. Molecular data show that transmission of P. matutinum (pLINN1) occurs broadly in the Holarctic, and the parasite likely is of cosmopolitan distribution. Passeriform birds and Culex mosquitoes are common hosts. This study provides first molecular markers for detection of P. matutinum.

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Epidemiology, clinical signs, histopathology and molecular characterization of canine leproid granuloma: a retrospective study of cases from Brazil

Veterinary Dermatology ◽

10.1111/j.1365-3164.2010.00934.x ◽

2011 ◽

Vol 22 (3) ◽

pp. 249-256 ◽

Cited By ~ 10

Author(s):

Lissandro Gonçalves Conceição ◽

Livia Maria Rosa Acha ◽

Alexandre Secorun Borges ◽

Fernanda G. Assis ◽

Fabricia Hallack Loures ◽

...

Keyword(s):

Retrospective Study ◽

Molecular Characterization ◽

Clinical Signs

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Renal Tumors of Childhood—A Histopathologic Pattern-Based Diagnostic Approach

Cancers ◽

10.3390/cancers12030729 ◽

2020 ◽

Vol 12 (3) ◽

pp. 729 ◽

Cited By ~ 2

Author(s):

Ariadne H.A.G. Ooms ◽

Gordan M. Vujanić ◽

Ellen D’Hooghe ◽

Paola Collini ◽

Aurore L’Herminé-Coulomb ◽

...

Keyword(s):

Molecular Characterization ◽

Molecular Analysis ◽

Pediatric Oncology ◽

Correct Diagnosis ◽

Renal Tumors ◽

Pediatric Tumors ◽

Cell Type ◽

Appropriate Treatment ◽

Histologic Pattern

Renal tumors comprise approximately 7% of all malignant pediatric tumors. This is a highly heterogeneous group of tumors, each with its own therapeutic management, outcome, and association with germline predispositions. Histopathology is the key in establishing the correct diagnosis, and therefore pathologists with expertise in pediatric oncology are needed for dealing with these rare tumors. While each tumor shows different histologic features, they do have considerable overlap in cell type and histologic pattern, making the diagnosis difficult to establish, if based on routine histology alone. To this end, ancillary techniques, such as immunohistochemistry and molecular analysis, can be of great importance for the correct diagnosis, resulting in appropriate treatment. To use ancillary techniques cost-effectively, we propose a pattern-based approach and provide recommendations to aid in deciding which panel of antibodies, supplemented by molecular characterization of a subset of genes, are required.

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Recovery and molecular characterization of live Camelpox virus from skin 12 months after onset of clinical signs reveals possible mechanism of virus persistence in herds

Veterinary Microbiology ◽

10.1016/j.vetmic.2012.04.022 ◽

2012 ◽

Vol 159 (3-4) ◽

pp. 320-326 ◽

Cited By ~ 8

Author(s):

A.A. Yousif ◽

A.A. Al-Naeem

Keyword(s):

Molecular Characterization ◽

Clinical Signs ◽

Virus Persistence

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Molecular Characterization of Coccidia Associated with an Epizootic in Green Sea Turtles (Chelonia mydas) in South East Queensland, Australia

PLoS ONE ◽

10.1371/journal.pone.0149962 ◽

2016 ◽

Vol 11 (2) ◽

pp. e0149962 ◽

Cited By ~ 15

Author(s):

Phoebe A. Chapman ◽

Helen Owen ◽

Mark Flint ◽

Rebecca J. Traub ◽

Thomas H. Cribb ◽

...

Keyword(s):

Molecular Characterization ◽

Sea Turtles ◽

Chelonia Mydas ◽

Green Sea Turtles

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Isolation and Molecular Characterization of Aspergillus fumigatus and Aspergillus flavus Isolated from Poultry Birds in Ado- Ekiti, Nigeria

Asian Journal of Biotechnology and Bioresource Technology ◽

10.9734/ajb2t/2020/v6i230078 ◽

2020 ◽

pp. 31-44

Author(s):

E. D. Fagbohun ◽

K. J. Ayantola ◽

A. J. Toyin-Famoroti

Keyword(s):

Aspergillus Fumigatus ◽

Molecular Characterization ◽

Aspergillus Flavus ◽

Clinical Signs ◽

Signs And Symptoms ◽

Aspergillus Species ◽

Exercise Intolerance ◽

State University ◽

Poultry Farms

Aim: The study was carried out to isolate and identify Aspergillus species from commercial birds with suspected aspergillosis in the poultry farms within Ado Ekiti metropolis Nigeria. Place and Period of Study: The study was carried out in the Department of Microbiology, Faculty of Science, Ekiti State University Ado Ekiti, Nigeria in August 2016. Methodology: A total of 35 sick/suspected birds were collected randomly from three poultry farms. At Ago-Aduloju poultry farms, 15 samples were randomly collected from 1000 birds while at Ekiti State University poultry farms, 10 samples were randomly collected from 500 birds. At Federal Polytechnic Ado Ekiti poultry farms, 10 samples were randomly collected from 700 birds. The bird’s selection was on the basis of clinical signs and symptoms such as difficulty in breathing, weight loss, drooping of wings and exercise intolerance. Swab samples were collected from each suspected/sick bird for mycological culture and molecular characterization of the isolates from each bird was carried out. The isolates were identified based on the color of the culture on Potato Dextrose Agar and microscopic examination. Molecular identification was done using 23S Ribosomal RNA Gene and Partial Sequence. Results: Six fungal strains that showed similar morphological and cultural characteristics of Aspergillus species were isolated. The isolates were coded ASP 1, ASP 2, ASP 3, ASP 4, ASP 5, and ASP 6. The identified organisms were; Aspergillus fumigatus qH 107 (ASP 1), Aspergillus fumigatus qH 107 (ASP 2), Aspergillus flavus M09 (ASP 3), Aspergillus flavus UOMS6 (ASP 4), Aspergillus fumigatus qH 107 (ASP 5), Aspergillus flavus qH 107 (ASP 6). Conclusion: It is evident that Aspergillus species were predominant in poultry farms selected in this study. Necessary precaution should be put in place to prevent the spread of aspergillosis. Poultry farmers are advised to avoid damp environments, moldy feeds, dry and dusty litters. Adequate ventilation should always be provided in poultry farms to prevent Aspergillosis.

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