Structural And Functional Analysis of CCT Family Genes In Pigeonpea

Abstract Pigeonpea (Cajanus cajan (L) Millsp) is a short-day plant in which the flowering is highly sensitive to photoperiod. A better understanding of the genes modulating photoresponse and flowering time is critical to developing photoperiod insensitive pigeonpea cultivars for cultivation across the seasons. We identified 33 CCT family genes (CcCCT1- CcCCT33) in C. cajan and localized them on 10 chromosomes and nine genomic scaffolds. The structural analysis of CCT family genes revealed a considerable variation in length and distribution of exons and introns. Based on the type of domain(s), we classified the CCT family genes into CCT motif family (CMF) type, CONSTANS like (COL) type, Pseudo-response Regulator (PRR) type, and GATA and tifi containing CCT (GTCC) type. The CCT family genes of C. cajan exhibited an extensive orthologous relationship with the CCT family genes of other legume species. We also observed significant sharing of CCT family genes among the legume species. Glycine max exhibited the maximum sharing of CCT family genes with C. cajan. The analysis of CCT family proteins-based phylogenic relationships revealed a general congruence with the legumes' taxonomic relationships. The expression analysis of CCT family genes of pigeonpea demonstrated that CcCCT4 and CcCCT23 are the active CONSTANS (CO) in ICP20338. In contrast, only CcCCT23 is active in MAL3, explaining the differential response of ICP20338 and MAL3 to photoperiod. The chromosomes of C. cajan contain a variable number of CCT family genes. A majority of these genes are localized in the centromeric regions. The COL type CCT genes are structurally highly diverse and contain a variable number of B-box domains. The CCT family genes of different legume species exhibit all three kinds of relationships: one-to-one, many-to-one, and many-to-many types. The photoperiod insensitive cultivar ICP20338 contains CcCCT4 and CcCCT23, while the photoperiod sensitive cultivar MAL3 contains only CcCCT23 as active CONSTANS (CO), which may be the plausible reason for their differential photoperiod response.

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Transcriptome Analysis to Identify Putative Genes Involved in Flowering Time Under Different Photoperiods in ‘Hong jin gou’ Common Bean

Journal of the American Society for Horticultural Science ◽

10.21273/jashs04719-19 ◽

2019 ◽

Vol 144 (4) ◽

pp. 274-279

Author(s):

Xiaoxu Yang ◽

Chang Liu ◽

Zhishan Yan ◽

Youjun Fan ◽

Guojun Feng ◽

...

Keyword(s):

Common Bean ◽

Flowering Time ◽

Response Regulator ◽

Differentially Expressed ◽

Day Length ◽

Rna Seq ◽

Yield And Quality ◽

Highly Sensitive ◽

Pseudo Response Regulator

Flowering time influences pod yield and quality of common bean (Phaseolus vulgaris); however, our knowledge of flowering time genes and flowering mechanisms in common bean remain limited. We performed RNA-sequencing (RNA-seq) analyses [long-day (LD) condition and short-day (SD) condition] to identify the flowering time genes and analyzed differentially expressed genes to examine their expression levels in relation to flowering time in ‘Hong Jin Gou’ common bean, a cultivar highly sensitive to photoperiod. The circadian patterns of related genes were identified using quantitative real-time polymerase chain reaction (qRT-PCR). Flowering time in ‘Hong Jin Gou’ was influenced by day length: SD conditions promoted flowering. A total of eight flowering time–related genes were identified, which were classified into photoperiod pathways. Homologs of pseudo-response regulator 5, pseudo-response regulator 7, and gigantea were more highly expressed under SD conditions than under LD conditions. Homologs of late elongated hypocotyl and timing of cab expression 1 were differentially expressed under light and dark conditions. Early flowering 3 is a key regulator of the pathway, which coordinates light and circadian clock inputs in leaves to trigger the expression of downstream genes. The present study provides critical information that could facilitate further investigations on the genetic mechanism of flowering time in common bean.

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Genome-wide characterization and expression analysis of pseudo-response regulator gene family in wheat

Molecular Biology Reports ◽

10.1007/s11033-021-06276-2 ◽

2021 ◽

Author(s):

Aliya Errum ◽

Nazia Rehman ◽

Muhammad Ramzan Khan ◽

Ghulam Muhammad Ali

Keyword(s):

Gene Family ◽

Expression Analysis ◽

Response Regulator ◽

Regulator Gene ◽

Genome Wide ◽

Pseudo Response Regulator

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Identification and characterization of a circadian clock-associated pseudo-response regulator 7 gene from trifoliate orange

Notulae Botanicae Horti Agrobotanici Cluj-Napoca ◽

10.15835/nbha48111785 ◽

2020 ◽

Vol 48 (1) ◽

pp. 128-139

Author(s):

Yu-E DING ◽

Wenkai HUANG ◽

Bo SHU ◽

Ying-Ning ZOU ◽

Qiang-Sheng WU ◽

...

Keyword(s):

Circadian Clock ◽

Response Regulator ◽

Poncirus Trifoliata ◽

Pcr Analysis ◽

Trifoliate Orange ◽

Drought Treatment ◽

Reading Frame ◽

Rhythmic Expression ◽

Pseudo Response Regulator ◽

Main Component

Circadian clock is usually involved in many physiological processes of plants, including responses to abiotic stress, whilst pseudo-response regulator 7 (PRR7) gene is the main component of the circadian clock. In this study, the cDNA of the PRR7 gene was obtained from trifoliate orange (Poncirus trifoliata). Based on the sequence analysis, the PtPRR7 gene had an open reading frame of 2343 bp, encoded 780 amino acids, and contained proteins of the REC and CCT domains. Subcellular localization indicated that PtPRR7 was mainly localized in the nucleus and a small amount of cytoplasm. qRT-PCR analysis revealed the highest expression level of PtPRR7 in roots than in both shoots and leaves. The PtPRR7 gene during 24 hours of soil water deficit exhibited a circadian rhythmic expression pattern: the expression peak at 9:00 am in leaves and at 21:00 pm in roots. Drought treatment affected PtPRR7 gene expression. Such data provide important references for understanding the characteristics of PtPRR7 gene in citrus plants.

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