scholarly journals Roles of ROS and Cell Cycle Arrest in the Genotoxicity Induced by Gold Nanorod Core/silver Shell Nanostructure 

2020 ◽  
Author(s):  
Dan Wang ◽  
Mo Dan ◽  
Yinglu Ji ◽  
Xiaochun Wu ◽  
Xue Wang ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Cell Cycle Arrest ◽  
Dna Cleavage ◽  
Micronucleus Test ◽  
Gold Nanorod ◽  
Chromosome Damage ◽  
Ion Release ◽  
Cycle Arrest ◽  
Heparg Cells ◽  
Silver Shell

Abstract To understand the genotoxicity induced in the liver by silver nanoparticles (AgNPs) and silver ions, an engineered gold nanorod core/silver shell nanostructure (Au@Ag NR) and humanized hepatocyte HepaRG cells were used in this study. The involvement of oxidative stress and cell cycle arrest in the DNA and chromosome damage induced by 0.4 - 20 µg.mL-1 Au@Ag NR were investigated by comet assay, γ-H2AX assay, and micronucleus test. Further, the distribution of Au@Ag NR was analyzed. Our results demonstrated that both Ag+ and Au@Ag NR led to DNA cleavage and chromosome damage (clastogenicity) in HepaRG cells, and that the Au@Ag NR retained in the nucleus may further release Ag+, aggravating the damages, which are mainly caused by cell cycle arrest and ROS formation. The results reveal the correlation between the intracellular accumulation, Ag+ ion release as well as the potential genotoxicity of AgNPs.

scholarly journals Roles of ROS and cell cycle arrest in the genotoxicity induced by gold nanorod core/silver shell nanostructure

2020 ◽  
Vol 15 (1) ◽  
Author(s):  
Dan Wang ◽  
Mo Dan ◽  
Yinglu Ji ◽  
Xiaochun Wu ◽  
Xue Wang ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Cell Cycle Arrest ◽  
Dna Cleavage ◽  
Micronucleus Test ◽  
Gold Nanorod ◽  
Chromosome Damage ◽  
Ion Release ◽  
Cycle Arrest ◽  
Heparg Cells ◽  
Silver Shell

AbstractTo understand the genotoxicity induced in the liver by silver nanoparticles (AgNPs) and silver ions, an engineered gold nanorod core/silver shell nanostructure (Au@Ag NR) and humanized hepatocyte HepaRG cells were used in this study. The involvement of oxidative stress and cell cycle arrest in the DNA and chromosome damage induced by 0.4–20 µg mL−1 Au@Ag NR were investigated by comet assay, γ-H2AX assay and micronucleus test. Further, the distribution of Au@Ag NR was analyzed. Our results demonstrated that both Ag+ and Au@Ag NR led to DNA cleavage and chromosome damage (clastogenicity) in HepaRG cells and that the Au@Ag NR retained in the nucleus may further release Ag+, aggravating the damages, which are mainly caused by cell cycle arrest and ROS formation. The results reveal the correlation between the intracellular accumulation, Ag+ ion release and the potential genotoxicity of AgNPs.

scholarly journals Rhein Induces Cell Death in HepaRG Cells through Cell Cycle Arrest and Apoptotic Pathway

2018 ◽  
Vol 19 (4) ◽  
pp. 1060 ◽  
Author(s):  
Longtai You ◽  
Xiaoxv Dong ◽  
Xingbin Yin ◽  
Chunjing Yang ◽  
Xin Leng ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Cell Death ◽  
Cell Cycle Arrest ◽  
Apoptotic Pathway ◽  
Cycle Arrest ◽  
Heparg Cells

scholarly journals Toxicity of Copper(I)-NHC Complexes Against Human Tumor Cells: Induction of Cell Cycle Arrest, Apoptosis, and DNA Cleavage

2008 ◽  
Vol 15 (2) ◽  
pp. 314-318 ◽  
Author(s):  
Marie-Laure Teyssot ◽  
Anne-Sophie Jarrousse ◽  
Aurélien Chevry ◽  
Angélique De Haze ◽  
Claude Beaudoin ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Cell Cycle Arrest ◽  
Tumor Cells ◽  
Dna Cleavage ◽  
Human Tumor ◽  
Human Tumor Cells ◽  
Cycle Arrest

Helicobacter pylori induces cell cycle arrest of T-lymphocytes

2005 ◽  
Vol 43 (05) ◽  
Author(s):  
M Gerhard ◽  
C Schmees ◽  
R Rad ◽  
P Voland ◽  
T Treptau ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Helicobacter Pylori ◽  
T Lymphocytes ◽  
Cell Cycle Arrest ◽  
Cycle Arrest

Mechanisms of interferon-induced cell cycle arrest

10.2741/a527 ◽  
2000 ◽  
Vol 5 (3) ◽  
pp. d479-487 ◽  
Author(s):  
Dan Grandér
Keyword(s):  
Cell Cycle ◽  
Cell Cycle Arrest ◽  
Cycle Arrest
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