scholarly journals Nitrogen-metabolising Microorganism Analysis in Rapid Sand Filters From Drinking Water Treatment Plant by Culturing and Metagenomics

2021 ◽  
Author(s):  
Qihui Gu ◽  
Jun Ma ◽  
Jumei Zhang ◽  
Weipeng Guo ◽  
Huiqing Wu ◽  
...  
Keyword(s):  
Drinking Water ◽  
Water Treatment ◽  
Removal Rate ◽  
Treatment Plant ◽  
Drinking Water Treatment ◽  
Water Treatment Plant ◽  
Amplicon Sequencing ◽  
Metabolic Model ◽  
Rrna Gene ◽  
N Removal

Abstract Sand filter (SFs) are common treatment processes for nitrogen pollutants removal in drinking water treatment plants (DWTPs). However, the mechanisms on the nitrogen-cycling role of SFs are still unclear. In this study, 16S rRNA gene amplicon sequencing was used to characterise the diversity and composition of the bacterial community in SFs from DWTPs. Additionally, metagenomics approach was used to determine the functional microorganisms involved in nitrogen cycle in SFs. Our results showed that Proteobacteria, Acidobacteria, Nitrospirae, and Chloroflexi dominated in SFs. Subsequently, 85 high-quality metagenome-assembled genomes (MAGs) were retrieved from metagenome datasets of selected SFs involving nitrification, assimilatory nitrogen reduction, and denitrification processes. Read mapping to reference genomes of Nitrospira and the phylogenetic tree of the ammonia monooxygenase subunit A gene, amoA, suggested that Nitrospira is abundantly found in SFs. Furthermore, according to their genetic content, a nitrogen metabolic model in SFs was proposed using representative MAGs and pure culture isolates. Quantitative real-time polymerase chain reaction (PCR) showed that ammonia-oxidising bacteria (AOB) and archaea (AOA), and complete ammonia oxidisers (comammox) were ubiquitous in the SFs, with the abundance of comammox being higher than that of AOA and AOB. Moreover, we identified a bacterial strain with a high NO3-N removal rate as Pseudomonas sp., which could be applied in the bioremediation of micro-polluted drinking water sources. Our study provides insights into functional nitrogen-metabolising microbes in SFs of DWTPs.

scholarly journals Bacillus purgationiresistans sp. nov., isolated from a drinking-water treatment plant

2012 ◽  
Vol 62 (1) ◽  
pp. 71-77 ◽  
Author(s):  
Ivone Vaz-Moreira ◽  
Vânia Figueira ◽  
Ana R. Lopes ◽  
Alexandre Lobo-da-Cunha ◽  
Cathrin Spröer ◽  
...  
Keyword(s):  
Drinking Water ◽  
Water Treatment ◽  
16S Rrna ◽  
16S Rrna Gene ◽  
Gene Sequence ◽  
Treatment Plant ◽  
Drinking Water Treatment ◽  
Water Treatment Plant ◽  
Rrna Gene ◽  
Rrna Gene Sequence

A Gram-positive, aerobic, non-motile, endospore-forming rod, designated DS22T, was isolated from a drinking-water treatment plant. Cells were catalase- and oxidase-positive. Growth occurred at 15–37 °C, at pH 7–10 and with <8 % (w/v) NaCl (optimum growth: 30 °C, pH 7–8 and 1–3 % NaCl). The major respiratory quinone was menaquinone 7, the G+C content of the genomic DNA was 36.5 mol% and the cell wall contained meso-diaminopimelic acid. On the basis of 16S rRNA gene sequence analysis, strain DS22T was a member of the genus Bacillus. Its closest phylogenetic neighbours were Bacillus horneckiae NRRL B-59162T (98.5 % 16S rRNA gene sequence similarity), Bacillus oceanisediminis H2T (97.9 %), Bacillus infantis SMC 4352-1T (97.4 %), Bacillus firmus IAM 12464T (96.8 %) and Bacillus muralis LMG 20238T (96.8 %). DNA–DNA hybridization, and biochemical and physiological characterization allowed the differentiation of strain DS22T from its closest phylogenetic neighbours. The data supports the proposal of a novel species, Bacillus purgationiresistans sp. nov.; the type strain is DS22T ( = DSM 23494T = NRRL B-59432T = LMG 25783T).

scholarly journals Effects of pre-ozonation and chemical coagulation on the removal of turbidity, color, TOC, and chlorophyll a from drinking water

2019 ◽  
Vol 6 (1) ◽  
pp. 53-61 ◽  
Author(s):  
Bahman Masoomi ◽  
Neamatollah Jaafarzadeh ◽  
Tayebeh Tabatabaie ◽  
Esmaeil Kouhgardi ◽  
Sahand Jorfi
Keyword(s):  
Drinking Water ◽  
Water Treatment ◽  
Chlorophyll A ◽  
Contact Time ◽  
Removal Rate ◽  
Treatment Plant ◽  
Drinking Water Treatment ◽  
Water Treatment Plant ◽  
Pilot Scale ◽  
Alkaline Ph

Background: Ozone can be used as a single technology or in combination with other processes to improve the coagulation- flocculation or biodegradability in order to remove pollutants in natural water treatment. Methods: In this study, the effects of pre-ozonation with coagulant substances on the quality parameters of drinking water were investigated using humic acid, kaolin, clay, and green algae in a pilot scale. This study was conducted under laboratory conditions (at both acidic and alkaline pH in different dosages of ozone and coagulant at ozone contact time with simulated water sample (5-20 minutes) in different scenarios). Results: The highest removal efficiency of parameters in the state of pre-ozonation alone and preozonation with a coagulant was observed at contact time of 20 minutes, ozone dosage of 5 g/h, coagulant dosage of 25 mg/L, at alkaline pH along with a decrease in temperature. So that, the average removal rate of turbidity, total organic carbon (TOC), color, and chlorophyll a in contact time of 20 minutes was 76.9%, 52.8%, 66.6%, and 85%, respectively. However, compared to ozonation under similar conditions, the reduction in turbidity, TOC, color, and chlorophyll a was 36.13%, 24.4%, 32.13%, and 79.6%, respectively. Also, it was revealed that pre-ozonation with coagulant could effectively improve the removal of parameters. Conclusion: However, since pre-ozonation can be effectively used to improve the coagulation efficacy in the drinking water treatment, the pre-ozonation combined with coagulation is proposed as an alternative to conventional coagulation to improve the process of drinking water treatment plant.

Experiences with the practical implementation and operation of biological nitrification for a small-scale drinking water treatment plant

2016 ◽  
Vol 16 (4) ◽  
pp. 922-930 ◽  
Author(s):  
L. Richard ◽  
E. Mayr ◽  
M. Zunabovic ◽  
R. Allabashi ◽  
R. Perfler
Keyword(s):  
Drinking Water ◽  
Water Treatment ◽  
Water Supply ◽  
Treatment Plant ◽  
Drinking Water Treatment ◽  
Water Treatment Plant ◽  
Drinking Water Supply ◽  
Small Scale ◽  
Treatment Performance ◽  
Biological Nitrification

The implementation and evaluation of biological nitrification as a possible treatment option for the small-scale drinking water supply of a rural Upper Austrian community was investigated. The drinking water supply of this community (average system input volume: 20 m3/d) is based on the use of deep anaerobic groundwater with a high ammonium content of geogenic origin (up to 5 mg/l) which must be treated to prevent the formation of nitrites in the drinking water supply system. This paper describes the implementation and operation of biological nitrification despite several constraints including space availability, location and financial and manpower resources. A pilot drinking water treatment plant, including biological nitrification implemented in sand filters, was designed and constructed for a maximum treatment capacity of 1.2 m3/h. Online monitoring of selected physicochemical parameters has provided continuous treatment performance data. Treatment performance of the plant was evaluated under standard operation as well as in the case of selected malfunction events.

scholarly journals Adsorption of Carbon Dioxide by Reusing Drinking Water Treatment Plant Sludge

2017 ◽  
Vol 29 (12) ◽  
pp. 2665-2670
Author(s):  
Soleha Mohamat Yusuff ◽  
K.K. Ong ◽  
W.M.Z. Wan Yunus ◽  
A. Fitrianto ◽  
M. Ahmad ◽  
...  
Keyword(s):  
Carbon Dioxide ◽  
Drinking Water ◽  
Water Treatment ◽  
Treatment Plant ◽  
Drinking Water Treatment ◽  
Water Treatment Plant ◽  
Drinking Water Treatment Plant ◽  
Water Treatment Plant Sludge

Control-design methodology for drinking-water treatment processes

2010 ◽  
Vol 10 (2) ◽  
pp. 121-127 ◽  
Author(s):  
Kim van Schagen ◽  
Luuk Rietveld ◽  
Alex Veersma ◽  
Robert Babuška
Keyword(s):  
Drinking Water ◽  
Water Treatment ◽  
Design Methodology ◽  
Treatment Plant ◽  
Control Design ◽  
Drinking Water Treatment ◽  
Water Treatment Plant ◽  
Process Characteristics ◽  
Chemical Usage ◽  
Treatment Step

The performance of a drinking-water treatment plant is determined by the control of the plant. To design the appropriate control system, a control-design methodology of five design steps is proposed, which takes the treatment process characteristics into account. For each design step, the necessary actions are defined. Using the methodology for the pellet-softening treatment step, a new control scheme for the pellet-softening treatment step has been designed and implemented in the full-scale plant. The implementation resulted in a chemical usage reduction of 15% and reduction in the maintenance effort for this treatment step. Corrective actions of operators are no longer necessary.

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