Functional Genomic Analysis of Breast Cancer Cell Tumorigenicity Using a Novel Gene Silencing Resource

2005 ◽  
Author(s):  
Douglas S. Conklin
Keyword(s):  
Breast Cancer ◽  
Gene Silencing ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Genomic Analysis ◽  
Functional Genomic ◽  
Novel Gene ◽  
Functional Genomic Analysis

Identification of a novel gene, DAM1, amplified at chromosome 1p13.3-21 region in human breast cancer cell lines

1999 ◽  
Vol 140 (1-2) ◽  
pp. 219-226 ◽  
Author(s):  
Koichi Nagasaki ◽  
Nicolai Maass ◽  
Tomohiro Manabe ◽  
Hiroaki Hanzawa ◽  
Toshihiko Tsukada ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cell Lines ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Human Breast Cancer ◽  
Cancer Cell Lines ◽  
Human Breast Cancer Cell ◽  
Breast Cancer Cell Lines ◽  
Human Breast ◽  
Novel Gene

scholarly journals The Cancer BioChip System: A Functional Genomic Assay for Anchorage-Independent Three-Dimensional Breast Cancer Cell Growth

2012 ◽  
Vol 3 (5-6) ◽  
pp. 261-270 ◽  
Author(s):  
Rula A. Abbud-Antaki ◽  
Joie N. Marhefka ◽  
Aimee L. DeLuca ◽  
Michael P. Zuromskis
Keyword(s):  
Breast Cancer ◽  
Cell Growth ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Three Dimensional ◽  
Functional Genomic ◽  
Cancer Cell Growth ◽  
Breast Cancer Cell Growth ◽  
System A

The effects of shRNA-mediated gene silencing of transcription factor SNAI1 on the biological phenotypes of breast cancer cell line MCF-7

2013 ◽  
Vol 388 (1-2) ◽  
pp. 113-121 ◽  
Author(s):  
Yan Lu ◽  
Lina Yu ◽  
Minlan Yang ◽  
Xiangshu Jin ◽  
Zhijing Liu ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Transcription Factor ◽  
Gene Silencing ◽  
Cell Line ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Breast Cancer Cell Line ◽  
Cancer Cell Line ◽  
Mcf 7

Abstract B67: Establishment of a high-content assay to assess the affects of gene silencing on inflammatory breast cancer cell motility

2010 ◽  
Author(s):  
Zainab I Thomas ◽  
Jonathan Z. Sexton ◽  
Willietta Gibson ◽  
Katherine M. Aird ◽  
Amy Aldrich ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Gene Silencing ◽  
Cell Motility ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Inflammatory Breast Cancer ◽  
Cancer Cell Motility ◽  
Inflammatory Breast Cancer Cell

scholarly journals Functional Genomic Analysis of Breast Cancer Metastasis: Implications for Diagnosis and Therapy

Cancers ◽  
2021 ◽  
Vol 13 (13) ◽  
pp. 3276
Author(s):  
Ziqi Yu ◽  
Mei Song ◽  
Lotfi Chouchane ◽  
Xiaojing Ma
Keyword(s):  
Breast Cancer ◽  
Cancer Metastasis ◽  
Genomic Analysis ◽  
Breast Cancer Metastasis ◽  
Prognostic Indicators ◽  
Genomic Sequencing ◽  
Functional Genomic ◽  
Therapeutic Implications ◽  
Sequencing Technologies ◽  
Functional Genomic Analysis

Breast cancer (BC) is one of the most diagnosed cancers worldwide and is the second cause of cancer related death in women. The most frequent cause of BC-related deaths, like many cancers, is metastasis. However, metastasis is a complicated and poorly understood process for which there is a shortage of accurate prognostic indicators and effective treatments. With the rapid and ever-evolving development and application of genomic sequencing technologies, many novel molecules were identified that play previously unappreciated and important roles in the various stages of metastasis. In this review, we summarize current advancements in the functional genomic analysis of BC metastasis and discuss about the potential prognostic and therapeutic implications from the recent genomic findings.

scholarly journals Genomic analysis of the HER2/TOP2A amplicon in breast cancer and breast cancer cell lines

2008 ◽  
Vol 88 (5) ◽  
pp. 491-503 ◽  
Author(s):  
Edurne Arriola ◽  
Caterina Marchio ◽  
David SP Tan ◽  
Suzanne C Drury ◽  
Maryou B Lambros ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cell Lines ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Genomic Analysis ◽  
Cancer Cell Lines ◽  
Breast Cancer Cell Lines

scholarly journals Functional Genomic Analyses of the 21q22.3 Locus Identifying Functional Variants and Candidate Gene YBEY for Breast Cancer Risk

Cancers ◽  
2021 ◽  
Vol 13 (9) ◽  
pp. 2037
Author(s):  
Chris Shidal ◽  
Xiang Shu ◽  
Jie Wu ◽  
Jifeng Wang ◽  
Shuya Huang ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Breast Cancer Risk ◽  
Cancer Risk ◽  
Cell Lines ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Breast Cancer Cell Lines ◽  
Functional Genomic ◽  
Functional Variants ◽  
Genomic Analyses

We previously identified a locus at 21q22.3, tagged by the single nucleotide polymorphism (SNP) rs35418111, being associated with breast cancer risk at a genome-wide significance level; however, the underlying causal functional variants and gene(s) responsible for this association are unknown. We performed functional genomic analyses to identify potential functional variants and target genes that may mediate this association. Functional annotation for SNPs in high linkage disequilibrium (LD, r2 > 0.8) with rs35418111 in Asians showed evidence of promoter and/or enhancer activities, including rs35418111, rs2078203, rs8134832, rs57385578, and rs8126917. These five variants were assessed for interactions with nuclear proteins by electrophoretic mobility shift assays. Our results showed that the risk alleles for rs2078203 and rs35418111 altered DNA-protein interaction patterns. Cis-expression quantitative loci (cis-eQTL) analysis, using data from the Genotype-Tissue Expression database (GTEx) European-ancestry female normal breast tissue, indicated that the risk allele of rs35418111 was associated with a decreased expression of the YBEY gene, a relatively uncharacterized endoribonuclease in humans. We investigated the biological effects of YBEY on breast cancer cell lines by transient knock-down of YBEY expression in MCF-7, T47D, and MDA-MB-231 cell lines. Knockdown of YBEY mRNA in breast cancer cell lines consistently decreased cell proliferation, colony formation, and migration/invasion, regardless of estrogen receptor status. We performed RNA sequencing in MDA-MB-231 cells transfected with siRNA targeting YBEY and subsequent gene set enrichment analysis to identify gene networks associated with YBEY knockdown. These data indicated YBEY was involved in networks associated with inflammation and metabolism. Finally, we showed trends in YBEY expression patterns in breast tissues from The Cancer Genome Atlas (TCGA); early-stage breast cancers had elevated YBEY expression compared with normal tissue, but significantly decreased expression in late-stage disease. Our study provides evidence of a significant role for the human YBEY gene in breast cancer pathogenesis and the association between the rs35418111/21q22.3 locus and breast cancer risk, which may be mediated through functional SNPs, rs35418111 and rs2078203, that regulate expression of YBEY.

Abstract 2107: Integrative molecular and functional genomic analysis of chemotherapy resistant bladder cancer cell lines identifies novel mediators of therapeutic response

2019 ◽  
Author(s):  
Robert T. Jones ◽  
Tahlita C. Zuiverloon ◽  
Hedvig Vekony ◽  
Andrew Goodspeed ◽  
Teemu D. Laajala ◽  
...  
Keyword(s):  
Bladder Cancer ◽  
Cell Lines ◽  
Cancer Cell ◽  
Therapeutic Response ◽  
Genomic Analysis ◽  
Cancer Cell Lines ◽  
Bladder Cancer Cell ◽  
Functional Genomic ◽  
Functional Genomic Analysis
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