scholarly journals Promotion of in Vitro Leaf Growth of Inner Scales Excised from Dormant Onion Bulbs

HortScience ◽  
1990 ◽  
Vol 25 (2) ◽  
pp. 228-229
Author(s):  
Kil Sun Yoo ◽  
Leonard M. Pike ◽  
B. Greg Cobb
Keyword(s):  
Growth Rate ◽  
Acetic Acid ◽  
Abscisic Acid ◽  
Gibberellic Acid ◽  
Allium Cepa ◽  
Leaf Growth ◽  
Inhibitory Effects ◽  
Short Day ◽  
Indole 3 Acetic Acid

Inner scales excised from dormant bulbs of the short-day `Texas Grano 1015Y' onion (Allium cepa L.) were cultured in vitro and leaf growth was examined. Light promoted leaf growth, but no differences in leaf growth were observed for media pH between 4 and 7. Leaf growth rate in darkness was highest at 24C, reduced at 15C, and greatly reduced at SC. Kinetin promoted leaf growth at 1, 10, and 100 μm. IAA was effective at 1 and 10 μM, but not at 0.1 and 100 μm. GA3 promoted growth at 0.1 μM. No inhibitory effects of ABA on leaf growth could be detected. Chemical names used: 1-H-indole-3-acetic acid (IAA), abscisic acid (ABA), gibberellic acid (GA3), 6-furfurylaminopurine (Kinetin).

scholarly journals Endogenous hormonal content during grain development in hexaploid and tetraploid wheat

1970 ◽  
Vol 33 (3) ◽  
pp. 493-502 ◽  
Author(s):  
Sridhar Gutam ◽  
Virendra Nath ◽  
GC Srivastava
Keyword(s):  
Growth Rate ◽  
Acetic Acid ◽  
Abscisic Acid ◽  
Gibberellic Acid ◽  
Grain Growth ◽  
Indole Acetic Acid ◽  
Tetraploid Wheat ◽  
Weight Basis ◽  
Ploidy Levels ◽  
Hormonal Content

A pot experiment was conducted in the rabi (post rainy) seasons of 2001 and 2002 to study the genotypic differences in grain growth rate and endogenous hormonal content in the developing grains of hexaploid and tetraploid wheat. The endogenous hormonal contents of grains in both the ploidy levels had changed in sequence. At 5 days after anthesis (DAA), gibberellic acid (GA3); at 15 DAA (rapid growth phase), indole-acetic acid (IAA); at 25 DAA (dough stage), abscisic acid (ABA) were maximum. At 35 DAA, all the endogenous hormonal level decreased and among the hormones, ABA was highest followed by IAA and GA3. Hexaploids recorded higher concentrations of endogenous hormones (13.38% IAA, 17.89% GA3, and 14.7% ABA) on fresh weight basis and resulted in higher seed weight (56.99 mg/grain) and grain growth rate (0.009 g/g/day) compared to tetraploids (49.08 mg/grain; 0.008 g/g/day) on dry weight basis by better mobilization of photosynthates during grain filling. Key Words: Grain growth rate, hormones, indole-acetic acid, gibberellic acid, abscisic acid. doi:10.3329/bjar.v33i3.1608 Bangladesh J. Agril. Res. 33(3) : 493-502, September 2008

scholarly journals Micropropagation of Mahonia ‘Soft Caress’

HortScience ◽  
2011 ◽  
Vol 46 (7) ◽  
pp. 1010-1014 ◽  
Author(s):  
Todd J. Rounsaville ◽  
Darren H. Touchell ◽  
Thomas G. Ranney ◽  
Frank A. Blazich
Keyword(s):  
Acetic Acid ◽  
Gibberellic Acid ◽  
Growth Regulator ◽  
Light Treatment ◽  
Ex Vitro ◽  
Basal Media ◽  
Basal Salts ◽  
Future Work ◽  
Indole 3 Acetic Acid

Mahonia ‘Soft Caress’ is a unique new cultivar exhibiting a compact form and delicate evergreen leaves. Protocols for micropropagation of M. ‘Soft Caress’ were developed to expedite multiplication and serve as a foundation for future work with other taxa of Mahonia Nutt. Combinations of sucrose at 30 or 45 g·L−1 in conjunction with Gamborg B5 (B5), Quoirin and Lepoivre (QL), and Murashige and Skoog (MS) basal media as well as other selected growth regulator treatments were evaluated as multiplication media. Rooting of microcuttings was conducted in vitro using combinations of indole-3-butyric acid (IBA) at 0, 2, 4, 8, or 16 μM under either light or dark. Quick dip treatments with aqueous solutions of the potassium (K) salt (K-salt) of IBA at 0, 5.2, 10.4, 20.7, or 41.4 μM were tested in a second experiment for ex vitro rooting. Media containing B5 basal salts and vitamins supplemented with sucrose at 30 g·L−1, 5 μM 6-benzylaminopurine, 5 μM kinetin, 0.5 μM indole-3-acetic acid, and 2.5 μM gibberellic acid yielded 2.80 ± 0.14 microshoots with a mean length of 14.76 ± 0.63 mm over a 6-week culture period and was an optimal multiplication media. Light treatment and IBA concentration had a significant effect on rooting percentages. Microcuttings treated with 8 μM IBA and maintained in the dark resulted in the best rooting (70%) and ex vitro establishment.

scholarly journals In vitro propagation of the endangered orchid Dendrobium chryseum Rolfefrom protocorms culture

2020 ◽  
Vol 19 (1) ◽  
pp. 39-47 ◽  
Author(s):  
Sabitri Maharjan ◽  
Laxmi Sen Thakuri ◽  
Bir Bahadur Thapa ◽  
Shreeti Pradhan ◽  
Krishna K. Pant ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Gibberellic Acid ◽  
In Vitro Propagation ◽  
Seedling Development ◽  
Naphthalene Acetic Acid ◽  
Mass Propagation ◽  
Endangered Orchid ◽  
Immature Seeds ◽  
Indole 3 Acetic Acid

 The immature seeds of Dendrobiumchryseum, asympodial epiphytic orchid with yellow flowers, were cultured in vitro, and the resultant protocorms were used as explants for seedling development. Protocorms were cultured on½ M.S. medium fortified with Kinetin (Kn), 6-Benzylaminopurine (BAP), and Gibberellic Acid (GA3) in three concentrations (0.5mg/l, 1.0mg/land 2.0 mg/l) both alone and supplemented with 5% and 10% coconut water (C.W.). The highest number of shootsofD. chryseum developed on ½ - M.S. medium fortified with 2.0mg/lofKn and10% C.W. and the longest shootsdeveloped on ½ M.S. media fortified with 1.0mg/lGA3, and 10% C.W. The shoot derived from protocorms were placed in ½ M.S. medium fortified with three different rooting hormones, Indole -3- acetic acid (IAA), Indole -3-butyric acid (IBA) and α-Naphthalene acetic acid (NAA) in different concentrations alone as well as with each 1.0mg/l hormone combined with 10% C.W. The most effective of these media was ½ M.S. medium fortified with 1.5 mg/l IAA for rooting as well as for the production of longest roots. The present study could be useful for standardizing the protocol for mass propagation of the endangered orchid Dendrobiumchryseum.

scholarly journals Endogenous levels of cytokinins, indole-3-acetic acid and abscisic acid in in vitro grown potato: A contribution to potato hormonomics

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Martin Raspor ◽  
Václav Motyka ◽  
Slavica Ninković ◽  
Petre I. Dobrev ◽  
Jiří Malbeck ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Abscisic Acid ◽  
Indole 3 Acetic Acid

scholarly journals Hormonal regulation of the growth of leaves and inflorescence stalk in Muscari armeniacum Leichtl.

2016 ◽  
Vol 69 (1) ◽  
Author(s):  
Marian Saniewski ◽  
Justyna Góraj-Koniarska ◽  
Elżbieta Węgrzynowicz-Lesiak ◽  
Eleonora Gabryszewska
Keyword(s):  
Acetic Acid ◽  
Abscisic Acid ◽  
Plant Growth ◽  
Gibberellic Acid ◽  
Growth Regulators ◽  
Hormonal Regulation ◽  
Present Experiment ◽  
Stem Growth ◽  
Flower Bud ◽  
Indole 3 Acetic Acid

It is known that chilling of <em>Muscari</em> bulbs is necessary for the growth of the inflorescence stalk and flowering, but not for the growth of leaves. Gibberellic acid (GA) accelerated stem growth and flowering in chilled <em>Muscari</em> bulbs. In the present experiment it was shown that in unchilled derooted <em>Muscari</em> bulbs the growth of leaves, but not the growth of the inflorescence stalk, was observed when bulbs were stored in water, GA at a concentration of 50 and 100 mg/L, benzyladenine (BA) at a concentration of 25 and 50 mg/L, or a mixture of GA+BA (50+25 mg/L), but abscisic acid (ABA) at a concentration of 10 mg/L greatly inhibited the growth of leaves. In chilled derooted <em>Muscari</em> bulbs the growth of leaves and inflorescence stalk was observed when bulbs were stored in water or GA, but BA and GA+BA treatments totally inhibited the growth of the inflorescence stalk without an effect on the growth of leaves. These results clearly showed that the growth of leaves and inflorescence stalk in <em>Muscari</em> bulbs are controlled by plant growth regulators in different ways. ABA totally inhibited the growth of leaves and inflorescence stalk in chilled derooted <em>Muscari</em> bulbs. It was shown that after the excision of the inflorescence bud in cultivated chilled <em>Muscari</em> bulbs, the inflorescence stalk died, but application of indole-3-acetic acid (IAA) 0.5% in the place of the removed inflorescence bud induced the growth of the inflorescence stalk. IAA applied under the inflorescence bud inhibited the development of flowers (flower-bud blasting) and induced the growth of the inflorescence stalk below the treatment site. These results are discussed with reference to hormonal regulation of stem (stalk) growth in tulip, narcissus, hyacinth, and <em>Hippeastrum</em>.

scholarly journals Poinsettia ‘Prestige™ Red’ (Euphorbia pulcherrima) In Vitro Propagation

HortScience ◽  
2010 ◽  
Vol 45 (7) ◽  
pp. 1126-1128
Author(s):  
Dinum Perera ◽  
Brian W. Trader
Keyword(s):  
Growth Rate ◽  
Acetic Acid ◽  
In Vitro Propagation ◽  
Basal Medium ◽  
Euphorbia Pulcherrima ◽  
In Vitro Proliferation ◽  
Slow Growth Rate ◽  
Half Strength ◽  
Indole 3 Acetic Acid

Slow growth rate of plantlets, few micro-shoots per explant, and slow root growth rate are restrictions of in vitro propagation of poinsettia (Euphorbia pulcherrima Willd. ex Koltz). The purpose of this research was to develop an efficient in vitro proliferation technique for poinsettia ‘Prestige™ Red’. Explants (apical buds and axillary buds) placed on Murashige and Skoog (MS) basal medium containing only 6-benzylaminopurine (BA) and combinations of BA and indole-3-acetic acid (IAA) mostly produced red callus, which is productive and some white and gray–green calluses at the base of plantlets after 1 month, whereas explants in a medium without plant growth regulators (PGRs) produced no callus. Addition of IAA into the rooting medium increased rooting efficiency; plantlets grown in half-strength MS salts and vitamins with 28.5 μM IAA initiated rooting 11 days earlier than the plantlets grown with no PGRs. Optimization of PGR concentrations during poinsettia micropropagation helped resolve previous restrictions of in vitro poinsettia proliferation. Chemical names used: 6-benzylaminopurine (BA); indole-3-acetic acid (IAA)

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