scholarly journals COLD ACCLIMATION IN THE LEAVES OF SIBLING DECIDUOUS AND EVERGREEN PEACH: ALTERATIONS IN DEHYDRIN AND BARK STORAGE PROTEINS

HortScience ◽  
1995 ◽  
Vol 30 (2) ◽  
pp. 190f-191
Author(s):  
Rajeev Arora ◽  
Michael Wisniewski ◽  
Lisa J. Rowland
Keyword(s):  
Cold Acclimation ◽  
Cold Tolerance ◽  
Prunus Persica ◽  
Storage Proteins ◽  
Seasonal Pattern ◽  
Bark Tissue ◽  
Bark Storage Proteins ◽  
Source Sink ◽  
Favorable Conditions ◽  
Bark Storage Protein

Seasonal pattern of cold tolerance and proteins were studied in the leaves of sibling deciduous and evergreen peach (Prunus persica). In contrast to deciduous peach that undergoes endodormancy in fall, evergreen peach does not (leaves are retained and shoot tips elongate under favorable conditions) (Arora et al., Plant Physiol. 99:1562-1568). Cold tolerance (LT50) was assessed using electrolyte leakage method. Proteins were separated by SDS-PAGE. Electroblots were probed with anti-dehydrin (Dr. T. Close) and anti-19 kD, peach bark storage protein (BSP) antibodies. LT50 of leaves successively increased from about -7C (18 Aug.) to -15C and -11.5C (23 Oct.) in deciduous and evergreen genotypes, respectively. The most apparent change in the protein profiles was the accumulation of a 60-kD protein during cold acclimation in the leaves of deciduous trees; however, it did not change significantly in evergreen peach. Immunoblots indicate that 60-kD protein is a dehydrin protein. PAGE and immunoblots indicated that 19-kD BSP disappeared progressively during summer through fall in the leaves of deciduous peach, but accumulated to large amounts in bark tissues. Similar inverse relationship for its accumulation in leaf vs. bark tissue was not evident in evergreen peach. Results indicate that BSP expression may be regulated by altered source/sink relationship.

scholarly journals Cold Acclimation and Alterations in Dehydrin-like and Bark Storage Proteins in the Leaves of Sibling Deciduous and Evergreen Peach

1996 ◽  
Vol 121 (5) ◽  
pp. 915-919 ◽  
Author(s):  
Rajeev Arora ◽  
Michael Wisniewski ◽  
Lisa J. Rowland
Keyword(s):  
Cold Acclimation ◽  
Gel Electrophoresis ◽  
Prunus Persica ◽  
Storage Proteins ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Subzero Temperature ◽  
Protein Gel ◽  
Bark Storage Proteins ◽  
High Level

Seasonal changes in cold tolerance and proteins were studied in the leaves of sibling deciduous and evergreen peach [Prunus persica (L.) Batsch]. Freezing tolerance [defined as the subzero temperature at which 50% injury occurred (LT50)] was assessed using electrolyte leakage. Proteins were separated by sodium dodecyl sulfate polyacrylamide-gel electrophoresis. Electroblots were probed with anti-dehydrin and anti-19-kD peach bark storage protein (BSP) antibodies. Leaf LT50 decreased successively from -5.8 °C on 18 Aug. to -10.3 °C in the evergreen genotype and from -7.0 °C to -15.0 °C in the deciduous genotype by 14 Oct. Protein profiles and immunoblots indicated the accumulation of a 60- and 30-kD protein during cold acclimation in the leaves of deciduous trees; however, levels of these proteins did not change significantly in the evergreen trees. Immunoblots indicate that the 60-kD protein is a dehydrin-like protein. Gel-electrophoresis and immunoblots also indicated that the 19-kD BSP progressively disappeared from summer through fall in leaves of deciduous peach but accumulated to a high level in bark tissues. A similar inverse relationship was not evident in evergreen peach.

scholarly journals Bark storage Protein in Peach, Plum, and Cherry Trees

HortScience ◽  
1995 ◽  
Vol 30 (4) ◽  
pp. 756A-756
Author(s):  
M.P. Bañados ◽  
M.S. Santiago ◽  
C. Eterovic
Keyword(s):  
Amino Acids ◽  
Storage Proteins ◽  
Protein Pattern ◽  
Fruit Trees ◽  
Sds Page ◽  
Bark Storage Proteins ◽  
Specific Proteins ◽  
Main Protein ◽  
Bark Storage Protein ◽  
Cherry Trees

The main form of nitrogen reserves during overwintering are amino acids and proteins. Specific proteins called bark storage proteins (BSP) have been characterized in many tree species. To identify BSPs in `O'Henry' peach, `Angeleno' plum, and `Early Burlat' cherry trees, samples of bark were collected from January through December 1993 from trees growing under field conditions in Santiago, Chile. SDS-PAGE analyses were used to characterize the seasonal variation on the protein pattern on the bark of those Prunus species. A 60 kDa BSP was identified in the bark of all three species, which corresponds to the main protein present in the bark during the winter. This protein may play an important role as a nitrogen reserve in these fruit trees.

scholarly journals Accumulation of a 60-kD Dehydrin Protein in Peach Xylem Tissues and Its Relationship to Cold Acclimation

HortScience ◽  
1996 ◽  
Vol 31 (6) ◽  
pp. 923-925 ◽  
Author(s):  
Rajeev Arora ◽  
Michael Wisniewski
Keyword(s):  
Cold Acclimation ◽  
Cold Hardiness ◽  
Prunus Persica ◽  
Seasonal Pattern ◽  
Seasonal Fluctuations ◽  
Accumulation Pattern ◽  
Early Stages

The seasonal pattern of dehydrin accumulation was characterized during cold acclimation and deacclimation in the xylem tissues of genetically related (sibling) deciduous and evergreen peach (Prunus persica L.). Immunological studies indicate that a 60-kD polypeptide in peach xylem tissues is a dehydrin protein. Comparison of its accumulation pattern with seasonal fluctuations in cold hardiness indicate that dehydrin accumulated to high levels during the peak of cold acclimation. However, its accumulation was only weakly associated with cold hardiness during early stages of cold acclimation and during deacclimation. Our results indicate that factors related to supercooling rather than dehydrin accumulation may be primarily responsible for determining levels of cold hardiness during transition periods.

Cold acclimation increases cold tolerance independently of diapause programing in the bean bug, Riptortus pedestris

2017 ◽  
Vol 108 (4) ◽  
pp. 487-493 ◽  
Author(s):  
J. Rozsypal ◽  
M. Moos ◽  
S.G. Goto
Keyword(s):  
Cold Acclimation ◽  
Cold Tolerance ◽  
Negative Impact ◽  
Riptortus Pedestris ◽  
Short Day ◽  
Cold Tolerant ◽  
Bean Bug ◽  
The Difference ◽  
Pass Through ◽  
Short Days

AbstractThe bean bug (Riptortus pedestris) is a pest of soybeans and other legumes in Japan and other Asian countries. It enters a facultative adult diapause on exposure to short days. While photoperiodism and diapause are well understood in R. pedestris, knowledge of cold tolerance is very limited, as is information on the effect of diapause on cold tolerance. We examined the effect of photoperiod, cold acclimation, and feeding status on cold tolerance in R. pedestris. We found that cold acclimation significantly increased survival at −10°C in both long- and short-day adult R. pedestris. Since the difference in cold survival between long- and short-day cold-acclimated groups was only marginal, we conclude that entering diapause is not crucial for R. pedestris to successfully pass through cold acclimation and become cold tolerant. We observed similar effects in 5th instar nymphs, with both long- and short-day cold-acclimated groups surviving longer cold exposures compared with non-acclimated groups. Starvation, which was tested only in adult bugs, had only a negligible and negative impact on cold survival. Although cold tolerance significantly increased with cold acclimation in adult bugs, supercooling capacity unexpectedly decreased. Our results suggest that changes in supercooling capacity as well as in water content are unrelated to cold tolerance in R. pedestris. An analysis of metabolites revealed differences between the treatments, and while several metabolites markedly increased with cold acclimation, their concentrations were too low to have a significant effect on cold tolerance.

Effects of cold acclimation and 6-OHDA sympathectomy on cold tolerance of rats

1979 ◽  
Vol 4 (3) ◽  
pp. 233-237 ◽  
Author(s):  
Marilyn Kiang-Ulrich ◽  
Steven M. Horvath
Keyword(s):  
Cold Acclimation ◽  
Cold Tolerance

Complex phytohormone responses during the cold acclimation of two wheat cultivars differing in cold tolerance, winter Samanta and spring Sandra

2012 ◽  
Vol 169 (6) ◽  
pp. 567-576 ◽  
Author(s):  
Klára Kosová ◽  
Ilja Tom Prášil ◽  
Pavel Vítámvás ◽  
Petre Dobrev ◽  
Václav Motyka ◽  
...  
Keyword(s):  
Cold Acclimation ◽  
Cold Tolerance ◽  
Wheat Cultivars

scholarly journals Characterization of Sequences Up-regulated in Peach Bark in Response to Low Temperature

HortScience ◽  
2005 ◽  
Vol 40 (4) ◽  
pp. 1130B-1130
Author(s):  
Carole L. Bassett ◽  
Robert E. Farrell ◽  
Timothy S. Artlip
Keyword(s):  
Signal Transduction ◽  
Seasonal Variation ◽  
Drought Stress ◽  
Low Temperature ◽  
Water Deficit ◽  
Prunus Persica ◽  
Subtractive Hybridization ◽  
Bark Tissue ◽  
Different Seasons

Genes whose expression is regulated by exposure to low temperature (LT) in peach (Prunus persica L. Batsch.) bark were identified by PCR suppression subtractive hybridization. Among the genes identified by this technique were several that had previously been associated with LT responsiveness, as well as a few that have not been reported to be regulated by cold. Genes represented by the first group included Ppdhn1, previously characterized as a seasonally expressed gene predominantly seen in bark tissue collected in winter months. A novel dehydrin found in this study, Ppdhn3, was also observed to be up-regulated at LT and seasonally expressed. Two genes not previously associated with LT response were found to be up-regulated at 5 °C. These genes encode a polypeptide related to some unknown mitochondrial process (Pptar1p) or a transducin-like protein (Pptlp1) that may be associated with signal transduction. Expression of these genes with respect to seasonal variation and drought stress is compared to genes from peach bark (Ppdhn1 and Ppdhn2), whose patterns of expression in different seasons and under water deficit are well documented.

scholarly journals Cold Acclimation Increases Freeze Tolerance in Acacia koa, a Tropical Tree Species Occurring over a Wide Elevational Gradient

Forests ◽  
2021 ◽  
Vol 12 (8) ◽  
pp. 1089
Author(s):  
Lilian M. Ayala-Jacobo ◽  
Keith E. Woeste ◽  
Douglass F. Jacobs
Keyword(s):  
Cold Acclimation ◽  
Cold Tolerance ◽  
Tree Species ◽  
Forest Restoration ◽  
Freeze Tolerance ◽  
Elevational Gradient ◽  
Frost Damage ◽  
Forest Tree ◽  
High Elevation ◽  
Acacia Koa

Frost damage is among the major limitations to reforestation and forest restoration projects worldwide. Investigations of environmental and genetic effects on frost resistance have focused on boreal and temperate tree species rather than tropical trees. Koa (Acacia koa A. Gray) is a valuable tropical hardwood tree species endemic to the Hawaiian Islands, USA. Koa occurs across a wide elevational gradient, and newly planted trees are subject to winter frost at high elevations. We sought to determine whether different koa populations show variation in freeze hardiness as a cold-tolerance mechanism, and whether exposure to hardening conditions prior to frost exposure can modify koa cold-tolerance adaptation. Seeds from 13 populations of koa (Acacia koa A. Gray) were collected across an elevational range (603–2050 m) on the Island of Hawai’i. Four-month-old seedlings grown from the 13 population seed sources were divided into control (non-acclimated) and cold-acclimated treatments, maintained at 26 °C/22 °C (day/night) or exposed to gradually decreasing temperatures to 8 °C/4 °C (day/night), respectively. After six weeks, control and cold-acclimated seedlings from each population were tested for freeze tolerance by electrolyte leakage at five test temperatures ranging from 5 °C (control) to −20 °C. Treatment effects were mainly observed at the lowest test temperatures (−15 and −20 °C). A higher index of cold damage occurred in the non-acclimated seedlings for most of the populations. Several of our higher elevation populations showed greater cold tolerance than populations from lower elevations, particularly when cold-acclimated. Our results suggest that cold acclimation may increase frost hardiness in a tropical forest tree species, and that there is likely some adaptive variation in frost tolerance among populations from different elevations. Cold acclimation could be a useful tool to prepare koa seedlings to be planted in high-elevation sites prone to freezing winter temperatures.

scholarly journals Transcriptome Analysis of Genes Involved in Cold Hardiness of Peach Tree (Prunus persica) Shoots during Cold Acclimation and Deacclimation

Genes ◽  
2020 ◽  
Vol 11 (6) ◽  
pp. 611
Author(s):  
Duk Jun Yu ◽  
Sung Hoon Jun ◽  
Junhyung Park ◽  
Jung Hyun Kwon ◽  
Hee Jae Lee
Keyword(s):  
Cold Acclimation ◽  
Transcriptome Analysis ◽  
Cold Hardiness ◽  
Prunus Persica ◽  
Quantitative Polymerase Chain Reaction ◽  
Differentially Expressed ◽  
Peach Tree ◽  
Triacylglycerol Lipase ◽  
Cold Hardy ◽  
Peach Trees

We analyzed the transcriptomes in the shoots of five-year-old ‘Soomee’ peach trees (Prunus persica) during cold acclimation (CA), from early CA (end of October) to late CA (middle of January), and deacclimation (DA), from late CA to late DA (middle of March), to identify the genes involved in cold hardiness. Cold hardiness of the shoots increased from early to late CA, but decreased from late CA to late DA, as indicated by decreased and increased the median lethal temperature (LT50), respectively. Transcriptome analysis identified 17,208 assembled transcripts during all three stages. In total, 1891 and 3008 transcripts were differentially expressed with a |fold change| > 2 (p < 0.05) between early and late CA, and between late CA and late DA, respectively. Among them, 1522 and 2830, respectively, were functionally annotated with gene ontology (GO) terms having a greater proportion of differentially expressed genes (DEGs) associated with molecular function than biological process or cellular component categories. The biochemical pathways best represented both periods from early to late CA and from late CA to late DA were ‘metabolic pathway’ and ‘biosynthesis of secondary metabolites’. We validated these transcriptomic results by performing reverse transcription quantitative polymerase chain reaction on the selected DEGs showing significant fold changes. The relative expressions of the selected DEGs were closely related to the LT50 values of the peach tree shoots: ‘Soomee’ shoots exhibited higher relative expressions of the selected DEGs than shoots of the less cold-hardy ‘Odoroki’ peach trees. Irrespective of the cultivar, the relative expressions of the DEGs that were up- and then down-regulated during CA, from early to late CA, and DA, from late CA to late DA, were more closely correlated with cold hardiness than those of the DEGs that were down- and then up-regulated. Therefore, our results suggest that the significantly up- and then down-regulated DEGs are associated with cold hardiness in peach tree shoots. These DEGs, including early light-induced protein 1, chloroplastic, 14-kDa proline-rich protein DC2.15, glutamate dehydrogenase 2, and triacylglycerol lipase 2, could be candidate genes to determine cold hardiness.

Sign in / Sign up

Export Citation Format

Share Document